Invitrogen

IgM Monoclonal Antibody (eB121-15F9), PE, eBioscience™

7 Published Figures

21 References

View all (30) Mouse IgM antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (7)

FIGURE: 1 / 8

IgM Antibody (12-5890-82) in Flow

Staining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) APC (Product # 17-0452-82) and 0.03 µg of Rat IgG2a kappa Isotype Control PE (Product # 12-4321-80) (left) or 0.03 µg of Anti-Mouse IgM PE (right). Total viable cells were used for analysis.

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Product Details

12-5890-82

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.125 µg/test

View 20 publications 20 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eB121-15F9

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466012

Product Specific Information

Description: The eB121-15F9 monoclonal antibody reacts with the mu heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated eB121-15F9 can be used as a detection secondary for mouse IgM.

Applications Reported: eB121-15F9 has been reported for use in flow cytometric analysis to detect the surface IgM on mouse B cells and as a second step to detect primary mouse IgMs.

Applications Tested: The eB121-15F9 antibody has been tested by flow cytometric analysis to detect the surface IgM on mouse B cells and can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Mouse IgM is an immunoglobulin M antibody that is produced by B cells in response to an antigen. It is the first antibody produced during an immune response and is the largest immunoglobulin in the serum. Mouse IgM is composed of five heavy chains and five light chains, and it is involved in complement activation and agglutination of antigens. It plays a crucial role in the innate immune response and is important for neutralizing pathogens before the adaptive immune response is fully activated. Mouse IgM is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Genetic alterations of the SUMO isopeptidase SENP6 drive lymphomagenesis and genetic instability in diffuse large B-cell lymphoma.

Nature communications

Schick M,Zhang L,Maurer S,Maurer HC,Isaakaidis K,Schneider L,Patra U,Schunck K,Rohleder E,Hofstetter J,Baluapuri A,Scherger AK,Slotta-Huspenina J,Hettler F,Weber J,Engleitner T,Maresch R,Slawska J,Lewis R,Istvanffy R,Habringer S,Steiger K,Baiker A,Oostendorp RAJ,Miething C,Lenhof HP,Bassermann F,Chapuy B,Wirth M,Wolf E,Rad R,Müller S,Keller U

12-5890-82 was used in Flow Cytometry to identify the SUMO isopeptidase SENP6 as a tumor suppressor that links unrestricted SUMOylation to tumor development and progression.

Wed Jan 12 00:00:00 EST 2022

Protective role of host complement system in Aspergillus fumigatus infection.

Frontiers in immunology

Shende R,Wong SSW,Meitei HT,Lal G,Madan T,Aimanianda V,Pal JK,Sahu A

12-5890-82 was used in flow cytometry to show that C3 knockout (C3-/-) mice are highly susceptible to systemic infection of A. fumigatus.

Tue Oct 11 00:00:00 EDT 2022

Image-seq: spatially resolved single-cell sequencing guided by in situ and in vivo imaging.

Nature methods

Haase C,Gustafsson K,Mei S,Yeh SC,Richter D,Milosevic J,Turcotte R,Kharchenko PV,Sykes DB,Scadden DT,Lin CP

12-5890-82 was used in Flow cytometry/Cell sorting to present Image-seq, a technology that provides single-cell transcriptional data on cells that are isolated from specific spatial locations under image guidance, thus preserving the spatial information of the target cells.

Thu Dec 01 00:00:00 EST 2022

Reciprocal regulation of RIG-I and XRCC4 connects DNA repair with RIG-I immune signaling.

Nature communications

Guo G,Gao M,Gao X,Zhu B,Huang J,Tu X,Kim W,Zhao F,Zhou Q,Zhu S,Wu Z,Yan Y,Zhang Y,Zeng X,Zhu Q,Yin P,Luo K,Sun J,Deng M,Lou Z

Published figure using IgM monoclonal antibody (Product # 12-5890-82) in Flow Cytometry

Mon Apr 12 00:00:00 EDT 2021

Low-density lipoprotein receptor-deficient hepatocytes differentiated from induced pluripotent stem cells allow familial hypercholesterolemia modeling, CRISPR/Cas-mediated genetic correction, and productive hepatitis C virus infection.

Stem cell research & therapy

Caron J,Pène V,Tolosa L,Villaret M,Luce E,Fourrier A,Heslan JM,Saheb S,Bruckert E,Gómez-Lechón MJ,Nguyen TH,Rosenberg AR,Weber A,Dubart-Kupperschmitt A

12-5890-82 was used in Flow Cytometry to provides the first LDLR-null FH cell model and its corrected counterpart to study the regulation of cholesterol metabolism and host determinants of HCV life cycle, and a platform to screen drugs for treating dyslipidemia and HCV infection.

Mon Jul 29 00:00:00 EDT 2019

Variation in zygotic CRISPR/Cas9 gene editing outcomes generates novel reporter and deletion alleles at the Gdf11 locus.

Scientific reports

Goldstein JM,Valido A,Lewandowski JP,Walker RG,Mills MJ,Messemer KA,Besseling P,Lee KH,Wattrus SJ,Cho M,Lee RT,Wagers AJ

12-5890 was used in Flow cytometry/Cell sorting to confirm that one-step zygotic injections of CRISPR/Cas gene editing complexes provide a quick and powerful tool to generate gene-modified mouse models.

Mon Dec 09 00:00:00 EST 2019

Indoleamine 2,3-Dioxygenase 1, Increased in Human Gastric Pre-Neoplasia, Promotes Inflammation and Metaplasia in Mice and Is Associated With Type II Hypersensitivity/Autoimmunity.

Gastroenterology

El-Zaatari M,Bass AJ,Bowlby R,Zhang M,Syu LJ,Yang Y,Grasberger H,Shreiner A,Tan B,Bishu S,Leung WK,Todisco A,Kamada N,Cascalho M,Dlugosz AA,Kao JY

Published figure using IgM monoclonal antibody (Product # 12-5890-82) in Flow Cytometry

Mon Jan 01 00:00:00 EST 2018

Human herpesvirus-encoded kinase induces B cell lymphomas in vivo.

The Journal of clinical investigation

Anders PM,Montgomery ND,Montgomery SA,Bhatt AP,Dittmer DP,Damania B

12-5890 was used in Flow cytometry/Cell sorting to analyse the function of viral protein kinase in vivo.

Fri Jun 01 00:00:00 EDT 2018

miR-128 inhibits telomerase activity by targeting TERT mRNA.

Oncotarget

Guzman H,Sanders K,Idica A,Bochnakian A,Jury D,Daugaard I,Zisoulis DG,Pedersen IM

12-5890 was used in Immunocytochemistry-immunoflourescence to study the mechanisms by which miR-128 inhibits telomerase activity to inhibit tumourigenesis.

Fri Mar 02 00:00:00 EST 2018

Genome organization and chromatin analysis identify transcriptional downregulation of insulin-like growth factor signaling as a hallmark of aging in developing B cells.

Genome biology

Koohy H,Bolland DJ,Matheson LS,Schoenfelder S,Stellato C,Dimond A,Várnai C,Chovanec P,Chessa T,Denizot J,Manzano Garcia R,Wingett SW,Freire-Pritchett P,Nagano T,Hawkins P,Stephens L,Elderkin S,Spivakov M,Fraser P,Corcoran AE,Varga-Weisz PD

12-5890 was used in Flow cytometry/Cell sorting to profile gene expression and chromatin features genome-wide, in bone marrow pro-B and pre-B cells from young and aged mice.

Wed Sep 05 00:00:00 EDT 2018

NFATc1 supports imiquimod-induced skin inflammation by suppressing IL-10 synthesis in B cells.

Nature communications

Alrefai H,Muhammad K,Rudolf R,Pham DA,Klein-Hessling S,Patra AK,Avots A,Bukur V,Sahin U,Tenzer S,Goebeler M,Kerstan A,Serfling E

Published figure using IgM monoclonal antibody (Product # 12-5890-82) in Flow Cytometry

Wed May 25 00:00:00 EDT 2016

The GIY-YIG Type Endonuclease Ankyrin Repeat and LEM Domain-Containing Protein 1 (ANKLE1) Is Dispensable for Mouse Hematopoiesis.

PloS one

Braun J,Meixner A,Brachner A,Foisner R

12-5890 was used in Flow cytometry/Cell sorting to test whether ANKLE1 may contribute to Holliday junction resolution during DNA repair in mice.

Fri Aug 12 00:00:00 EDT 2016

Mutations in G protein β subunits promote transformation and kinase inhibitor resistance.

Nature medicine

Yoda A,Adelmant G,Tamburini J,Chapuy B,Shindoh N,Yoda Y,Weigert O,Kopp N,Wu SC,Kim SS,Liu H,Tivey T,Christie AL,Elpek KG,Card J,Gritsman K,Gotlib J,Deininger MW,Makishima H,Turley SJ,Javidi-Sharifi N,Maciejewski JP,Jaiswal S,Ebert BL,Rodig SJ,Tyner JW,Marto JA,Weinstock DM,Lane AA

12589082 was used in flow cytometry to investigate the role of GNB1 and GNB2 in cancer cells.

Thu Jan 01 00:00:00 EST 2015

B cell-intrinsic CD84 and Ly108 maintain germinal center B cell tolerance.

Journal of immunology (Baltimore, Md. : 1950)

Wong EB,Soni C,Chan AY,Domeier PP,Abraham T,Limaye N,Khan TN,Elias MJ,Chodisetti SB,Wakeland EK,Rahman ZS

12-5890 was used in Flow cytometry/Cell sorting to investigate the effect of B cell-intrinsic polymorphic SLAM receptors on germinal center B cell tolerance.

Fri May 01 00:00:00 EDT 2015

Essential role for autophagy in the maintenance of immunological memory against influenza infection.

Nature medicine

Chen M,Hong MJ,Sun H,Wang L,Shi X,Gilbert BE,Corry DB,Kheradmand F,Wang J

12-5890 was used in Flow cytometry/Cell sorting to demonstrate autophagy is essential for the survival of virus-specific memory B cells and maintenance of protective antibody responses.

Thu May 01 00:00:00 EDT 2014

Murine complement receptor 1 is required for germinal center B cell maintenance but not initiation.

Immunobiology

Donius LR,Weis JJ,Weis JH

12-5890 was used in Flow cytometry/Cell sorting to describe unique functions of Cr1 on the surface of both B cells and follicular dendritic cells.

Sun Jun 01 00:00:00 EDT 2014

Generation of a novel Cr2 gene allele by homologous recombination that abrogates production of Cr2 but is sufficient for expression of Cr1.

Immunobiology

Donius LR,Orlando CM,Weis JJ,Weis JH

12-5890 was used in Flow cytometry/Cell sorting to study how the Cr2KO mouse provides a new model system for the analysis of Cr1 and Cr2 functions in the immune response of the mouse.

Wed Jan 01 00:00:00 EST 2014

The histone methyltransferase MMSET regulates class switch recombination.

Journal of immunology (Baltimore, Md. : 1950)

Pei H,Wu X,Liu T,Yu K,Jelinek DF,Lou Z

Published figure using IgM monoclonal antibody (Product # 12-5890-82) in Flow Cytometry

Tue Jan 15 00:00:00 EST 2013

Y chromosome-linked B and NK cell deficiency in mice.

Journal of immunology (Baltimore, Md. : 1950)

Sun SL,Horino S,Itoh-Nakadai A,Kawabe T,Asao A,Takahashi T,So T,Funayama R,Kondo M,Saitsu H,Matsumoto N,Nakayama K,Ishii N

12-5890 was used in Flow cytometry/Cell sorting to establish a novel mouse strain in which all males lack B and natural killer cells and have Peyer's patch defects.

Sat Jun 15 00:00:00 EDT 2013

The inhibiting Fc receptor for IgG, FcγRIIB, is a modifier of autoimmune susceptibility.

Journal of immunology (Baltimore, Md. : 1950)

Boross P,Arandhara VL,Martin-Ramirez J,Santiago-Raber ML,Carlucci F,Flierman R,van der Kaa J,Breukel C,Claassens JW,Camps M,Lubberts E,Salvatori D,Rastaldi MP,Ossendorp F,Daha MR,Cook HT,Izui S,Botto M,Verbeek JS

12-5890 was used in Flow cytometry/Cell sorting to determine the specific contribution of FcγRIIB deficiency to the development of lupus in FcγRIIB(129)(-/-) mice.

Mon Aug 01 00:00:00 EDT 2011

A possible contribution of retinoids to regulation of fetal B lymphopoiesis.

European journal of immunology

Chen X,Welner RS,Kincade PW

12-5890 was used in Flow cytometry/Cell sorting to determine that retinoids may partially account for rapid differentiation in foetal life, but not for all unique features of foetal progenitors.

Tue Sep 01 00:00:00 EDT 2009

Bioinformatics

Gene Aliases: AI326478; Igh-6; Igh-M; Igh6; Igm; muH

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Entrez Gene ID: (Mouse) 16019

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Function(s)

immunoglobulin

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IgM Monoclonal Antibody (eB121-15F9), PE, eBioscience™

IgM Antibody (12-5890-82) in Flow

Product Details

12-5890-82

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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