Invitrogen

IgM Monoclonal Antibody (eB121-15F9), eBioscience™

7 Published Figures

14 References

View all (30) Mouse IgM antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Published Figures (7)

FIGURE: 1 / 7

IgM Antibody (14-5890-82) in Flow

Nature communications 2021 - Fig. 1 RIG-I is recruited to DNA DSB sites and suppresses non-homologous end-joining. a A549 cells were treated with irradiation (IR, 10 Gy, 2 h). RIG-I protein levels in the cytosolic (C) and nuclear (N) fractions were detected by Western blot. b A549 cells were treated with IR (10 Gy) for the indicated times. RIG-I protein levels in the soluble and chromatin fractions were examined by Western blot. c ER-A... View More

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Product Details

14-5890-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

View 13 publications 13 publications

ELISA (ELISA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

eB121-15F9

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_467686

Product Specific Information

Description: The eB121-15F9 monoclonal antibody reacts with the mu heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated eB121-15F9 can be used as a detection secondary for mouse IgM.

Applications Reported: eB121-15F9 has been reported for use in flow cytometric analysis to detect the surface IgM on mouse B cells and as a second step to detect primary mouse IgMs. It can also be used in ELISA.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Mouse IgM is an immunoglobulin M antibody that is produced by B cells in response to an antigen. It is the first antibody produced during an immune response and is the largest immunoglobulin in the serum. Mouse IgM is composed of five heavy chains and five light chains, and it is involved in complement activation and agglutination of antigens. It plays a crucial role in the innate immune response and is important for neutralizing pathogens before the adaptive immune response is fully activated. Mouse IgM is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Reciprocal regulation of RIG-I and XRCC4 connects DNA repair with RIG-I immune signaling.

Nature communications

Guo G,Gao M,Gao X,Zhu B,Huang J,Tu X,Kim W,Zhao F,Zhou Q,Zhu S,Wu Z,Yan Y,Zhang Y,Zeng X,Zhu Q,Yin P,Luo K,Sun J,Deng M,Lou Z

Published figure using IgM monoclonal antibody (Product # 14-5890-82) in Flow Cytometry

Mon Apr 12 00:00:00 EDT 2021

Indoleamine 2,3-Dioxygenase 1, Increased in Human Gastric Pre-Neoplasia, Promotes Inflammation and Metaplasia in Mice and Is Associated With Type II Hypersensitivity/Autoimmunity.

Gastroenterology

El-Zaatari M,Bass AJ,Bowlby R,Zhang M,Syu LJ,Yang Y,Grasberger H,Shreiner A,Tan B,Bishu S,Leung WK,Todisco A,Kamada N,Cascalho M,Dlugosz AA,Kao JY

Published figure using IgM monoclonal antibody (Product # 14-5890-82) in Flow Cytometry

Mon Jan 01 00:00:00 EST 2018

Cleavage of TL1A Differentially Regulates Its Effects on Innate and Adaptive Immune Cells.

Journal of immunology (Baltimore, Md. : 1950)

Ferdinand JR,Richard AC,Meylan F,Al-Shamkhani A,Siegel RM

14-5890 was used in Flow cytometry/Cell sorting to suggest differential roles for membrane and soluble TL1A on adaptive and innate immune cells and have implications for the consequences of blocking these two forms of TL1A.

Thu Feb 15 00:00:00 EST 2018

Defective germinal center B-cell response and reduced arthritic pathology in microRNA-29a-deficient mice.

Cellular and molecular life sciences : CMLS

van Nieuwenhuijze A,Dooley J,Humblet-Baron S,Sreenivasan J,Koenders M,Schlenner SM,Linterman M,Liston A

14589082 was used in flow cytometry to evaluate miR-29a in B cells as a potential therapeutic target in arthritis

Thu Jun 01 00:00:00 EDT 2017

The miR-15 family reinforces the transition from proliferation to differentiation in pre-B cells.

EMBO reports

Lindner SE,Lohmüller M,Kotkamp B,Schuler F,Knust Z,Villunger A,Herzog S

14-5890 was used in Flow cytometry/Cell sorting to identify the miR-15 family as a novel element required to promote the switch from pre-B cell proliferation to differentiation.

Fri Sep 01 00:00:00 EDT 2017

T_fr cells lack IL-2Rα but express decoy IL-1R2 and IL-1Ra and suppress the IL-1-dependent activation of T_fh cells.

Science immunology

Ritvo PG,Churlaud G,Quiniou V,Florez L,Brimaud F,Fourcade G,Mariotti-Ferrandiz E,Klatzmann D

14-5890-82 was used in ELISA to reveal an IL-1 axis in the Tfh cell control of B cell responses and an IL-2/IL-1 dichotomy for Treg cell control of effector T cells versus Tfr cell control of Tfh cells.

Fri Sep 08 00:00:00 EDT 2017

MAP3K11 is a tumor suppressor targeted by the oncomiR miR-125b in early B cells.

Cell death and differentiation

Knackmuss U,Lindner SE,Aneichyk T,Kotkamp B,Knust Z,Villunger A,Herzog S

14589082 was used in flow cytometry to find and elucidate a role for miR-125b in B-cell leukemia

Mon Feb 01 00:00:00 EST 2016

NFATc1 supports imiquimod-induced skin inflammation by suppressing IL-10 synthesis in B cells.

Nature communications

Alrefai H,Muhammad K,Rudolf R,Pham DA,Klein-Hessling S,Patra AK,Avots A,Bukur V,Sahin U,Tenzer S,Goebeler M,Kerstan A,Serfling E

Published figure using IgM monoclonal antibody (Product # 14-5890-82) in Flow Cytometry

Wed May 25 00:00:00 EDT 2016

Ly49 family receptors are required for cancer immunosurveillance mediated by natural killer cells.

Cancer research

Tu MM,Mahmoud AB,Wight A,Mottashed A,Bélanger S,Rahim MM,Abou-Samra E,Makrigiannis AP

14-5890 was used in Flow cytometry/Cell sorting to evaluate the requirement of NK cells in innate immune surveillance of cancer cells, showing that Ly49 family receptors are required.

Tue Jul 15 00:00:00 EDT 2014

mTOR kinase inhibitors promote antibody class switching via mTORC2 inhibition.

Proceedings of the National Academy of Sciences of the United States of America

Limon JJ,So L,Jellbauer S,Chiu H,Corado J,Sykes SM,Raffatellu M,Fruman DA

14-5890 was used in Flow cytometry/Cell sorting to further elucidate the complexity of mTOR signalling in B cells.

Tue Nov 25 00:00:00 EST 2014

Innate and adaptive type 2 immune cell responses in genetically controlled resistance to intestinal helminth infection.

Immunology and cell biology

Filbey KJ,Grainger JR,Smith KA,Boon L,van Rooijen N,Harcus Y,Jenkins S,Hewitson JP,Maizels RM

14-5890 was used in Flow cytometry/Cell sorting to study the differential levels of resistance to the nematode, H. polygyrus, that are observable between mouse models.

Wed Jan 07 00:00:00 EST 2015

The histone methyltransferase MMSET regulates class switch recombination.

Journal of immunology (Baltimore, Md. : 1950)

Pei H,Wu X,Liu T,Yu K,Jelinek DF,Lou Z

Published figure using IgM monoclonal antibody (Product # 14-5890-82) in Flow Cytometry

Tue Jan 15 00:00:00 EST 2013

Critical role of SAP in progression and reactivation but not maintenance of T cell-dependent humoral immunity.

Molecular and cellular biology

Zhong MC,Veillette A

14-5890 was used in Flow cytometry/Cell sorting to explore the therapeutic potential of blocking the SLAM-associated protein (SAP) for human autoimmune diseases.

Fri Mar 01 00:00:00 EST 2013

Role of mast cells and basophils in IgE responses and in allergic airway hyperresponsiveness.

Journal of immunology (Baltimore, Md. : 1950)

Sawaguchi M,Tanaka S,Nakatani Y,Harada Y,Mukai K,Matsunaga Y,Ishiwata K,Oboki K,Kambayashi T,Watanabe N,Karasuyama H,Nakae S,Inoue H,Kubo M

14-5890 was used in Flow cytometry/Cell sorting to demonstrate an indispensable role for MCs as effector cells in AHR.

Wed Feb 15 00:00:00 EST 2012

Bioinformatics

Gene Aliases: AI326478; Igh-6; Igh-M; Igh6; Igm; muH

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Entrez Gene ID: (Mouse) 16019

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Function(s)

immunoglobulin

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IgM Monoclonal Antibody (eB121-15F9), eBioscience™

IgM Antibody (14-5890-82) in Flow

Product Details

14-5890-82

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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