Invitrogen

IgM Monoclonal Antibody (eB121-15F9), FITC, eBioscience™

8 Published Figures

19 References

View all (30) Mouse IgM antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (8)

FIGURE: 1 / 9

IgM Antibody (11-5890-82) in Flow

Staining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) APC (Product # 17-0452-82) and 0.25 µg of Rat IgG2a kappa Isotype Control FITC (Product # 11-4321-42) (left) or 0.25 µg of Anti-Mouse IgM FITC (right). Total viable cells were used for analysis.

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Product Details

11-5890-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 19 publications 19 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eB121-15F9

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465292

Product Specific Information

Description: The eB121-15F9 monoclonal antibody reacts with the mu heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated eB121-15F9 can be used as a detection secondary for mouse IgM.

Applications Reported: eB121-15F9 has been reported for use in flow cytometric analysis to detect the surface IgM on mouse B cells and as a second step to detect primary mouse IgMs.

Applications Tested: The eB121-15F9 antibody has been tested by flow cytometric analysis to detect the surface IgM on mouse B cells and can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Mouse IgM is an immunoglobulin M antibody that is produced by B cells in response to an antigen. It is the first antibody produced during an immune response and is the largest immunoglobulin in the serum. Mouse IgM is composed of five heavy chains and five light chains, and it is involved in complement activation and agglutination of antigens. It plays a crucial role in the innate immune response and is important for neutralizing pathogens before the adaptive immune response is fully activated. Mouse IgM is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

The Wdr1-LIMK-Cofilin Axis Controls B Cell Antigen Receptor-Induced Actin Remodeling and Signaling at the Immune Synapse.

Frontiers in cell and developmental biology

Bolger-Munro M,Choi K,Cheung F,Liu YT,Dang-Lawson M,Deretic N,Keane C,Gold MR

11-5890 was used in Flow cytometry/Cell sorting to show that Wdr1 and LIMK have distinct roles in BCR-induced assembly of the peripheral actin structures that drive B cell spreading, and that cofilin, Wdr1, and LIMK all contribute to the actin-dependent amplification of BCR signaling at the immune synapse.

Sun May 02 00:00:00 EDT 2021

Reciprocal regulation of RIG-I and XRCC4 connects DNA repair with RIG-I immune signaling.

Nature communications

Guo G,Gao M,Gao X,Zhu B,Huang J,Tu X,Kim W,Zhao F,Zhou Q,Zhu S,Wu Z,Yan Y,Zhang Y,Zeng X,Zhu Q,Yin P,Luo K,Sun J,Deng M,Lou Z

Published figure using IgM monoclonal antibody (Product # 11-5890-82) in Flow Cytometry

Mon Apr 12 00:00:00 EDT 2021

The cis-Regulatory Atlas of the Mouse Immune System.

Cell

Yoshida H,Lareau CA,Ramirez RN,Rose SA,Maier B,Wroblewska A,Desland F,Chudnovskiy A,Mortha A,Dominguez C,Tellier J,Kim E,Dwyer D,Shinton S,Nabekura T,Qi Y,Yu B,Robinette M,Kim KW,Wagers A,Rhoads A,Nutt SL,Brown BD,Mostafavi S,Buenrostro JD,Benoist C

11-5890-82 was used in Flow Cytometry to generate matched epigenome and transcriptome measurements in 86 primary cell types that span the mouse immune system and its differentiation cascade.

Thu Feb 07 00:00:00 EST 2019

Single-nucleotide human disease mutation inactivates a blood-regenerative GATA2 enhancer.

The Journal of clinical investigation

Soukup AA,Zheng Y,Mehta C,Wu J,Liu P,Cao M,Hofmann I,Zhou Y,Zhang J,Johnson KD,Choi K,Keles S,Bresnick EH

11-5890 was used in Flow cytometry/Cell sorting to study the mechanisms underlying how GATA2 enhancer disease mutations impact hematopoiesis and pathology.

Fri Mar 01 00:00:00 EST 2019

ImmGen report: sexual dimorphism in the immune system transcriptome.

Nature communications

Gal-Oz ST,Maier B,Yoshida H,Seddu K,Elbaz N,Czysz C,Zuk O,Stranger BE,Ner-Gaon H,Shay T

11-5890 was used in Flow cytometry/Cell sorting to show transcriptional sexual dimorphism in macrophages.

Fri Sep 20 00:00:00 EDT 2019

Indoleamine 2,3-Dioxygenase 1, Increased in Human Gastric Pre-Neoplasia, Promotes Inflammation and Metaplasia in Mice and Is Associated With Type II Hypersensitivity/Autoimmunity.

Gastroenterology

El-Zaatari M,Bass AJ,Bowlby R,Zhang M,Syu LJ,Yang Y,Grasberger H,Shreiner A,Tan B,Bishu S,Leung WK,Todisco A,Kamada N,Cascalho M,Dlugosz AA,Kao JY

Published figure using IgM monoclonal antibody (Product # 11-5890-82) in Flow Cytometry

Mon Jan 01 00:00:00 EST 2018

L3MBTL2 orchestrates ubiquitin signalling by dictating the sequential recruitment of RNF8 and RNF168 after DNA damage.

Nature cell biology

Nowsheen S,Aziz K,Aziz A,Deng M,Qin B,Luo K,Jeganathan KB,Zhang H,Liu T,Yu J,Deng Y,Yuan J,Ding W,van Deursen JM,Lou Z

11-5890-82 was used in Flow Cytometry to investigate what dictates the sequential recruitment of E3 ubiquitin ligases RNF8 and RNF168 following DNA damage.

Sun Apr 01 00:00:00 EDT 2018

RNA Helicase DDX1 Converts RNA G-Quadruplex Structures into R-Loops to Promote IgH Class Switch Recombination.

Molecular cell

Ribeiro de Almeida C,Dhir S,Dhir A,Moghaddam AE,Sattentau Q,Meinhart A,Proudfoot NJ

11-5890 was used in Flow cytometry/Cell sorting to provide evidence for how S-region transcripts interconvert between G4 and R-loop structures to promote IgH class switch recombination.

Thu May 17 00:00:00 EDT 2018

Human leukemia mutations corrupt but do not abrogate GATA-2 function.

Proceedings of the National Academy of Sciences of the United States of America

Katsumura KR,Mehta C,Hewitt KJ,Soukup AA,Fraga de Andrade I,Ranheim EA,Johnson KD,Bresnick EH

11-5890 was used in Flow cytometry/Cell sorting to determine whether distinct mutations differentially impact GATA 2 mechanisms.

Tue Oct 23 00:00:00 EDT 2018

ZNF506-dependent positive feedback loop regulates H2AX signaling after DNA damage.

Nature communications

Nowsheen S,Aziz K,Luo K,Deng M,Qin B,Yuan J,Jeganathan KB,Yu J,Zhang H,Ding W,van Deursen JM,Lou Z

11-5890 was used in Flow cytometry/Cell sorting to elucidate how the DNA damage response pathway is orchestrated by ZNF506 to maintain genomic integrity.

Mon Jul 16 00:00:00 EDT 2018

The IgH locus 3' cis-regulatory super-enhancer co-opts AID for allelic transvection.

Oncotarget

Le Noir S,Laffleur B,Carrion C,Garot A,Lecardeur S,Pinaud E,Denizot Y,Skok J,Cogné M

11-5890 was used in Flow cytometry/Cell sorting to show that under physiological conditions, the 3'RR super-enhancer supports not only legitimate cis- , but also trans-recruitment of AID, contributing to IgH inter-allelic proximity and enabling the super-enhancer on one allele to stimulate biallelic SHM and CSR.

Tue Feb 21 00:00:00 EST 2017

NFATc1 supports imiquimod-induced skin inflammation by suppressing IL-10 synthesis in B cells.

Nature communications

Alrefai H,Muhammad K,Rudolf R,Pham DA,Klein-Hessling S,Patra AK,Avots A,Bukur V,Sahin U,Tenzer S,Goebeler M,Kerstan A,Serfling E

11-5890 was used in Flow cytometry/Cell sorting to indicate a close link between NFATc1 and IL-10 expression in B cells, suggesting that NFATc1 may be a potential target to treat human psoriasis.

Wed May 25 00:00:00 EDT 2016

V(D)J recombination process and the Pre-B to immature B-cells transition are altered in Fanca^-/- mice.

Scientific reports

Nguyen TV,Pawlikowska P,Firlej V,Rosselli F,Aoufouchi S

11-5890 was used in Flow cytometry/Cell sorting to determine the impact of the FANC pathway on the immunoglobulin diversification process.

Thu Nov 24 00:00:00 EST 2016

Cis-regulatory mechanisms governing stem and progenitor cell transitions.

Science advances

Johnson KD,Kong G,Gao X,Chang YI,Hewitt KJ,Sanalkumar R,Prathibha R,Ranheim EA,Dewey CN,Zhang J,Bresnick EH

11-5890 was used in Flow cytometry/Cell sorting to illustrate a paradigm in which cis-elements in a locus differentially control stem and progenitor cell transitions.

Tue Sep 01 00:00:00 EDT 2015

Oral combined therapy with probiotics and alloantigen induces B cell-dependent long-lasting specific tolerance.

Journal of immunology (Baltimore, Md. : 1950)

Mercadante AC,Perobelli SM,Alves AP,Gonçalves-Silva T,Mello W,Gomes-Santos AC,Miyoshi A,Azevedo V,Faria AM,Bonomo A

11-5890 was used in Flow cytometry/Cell sorting to suggest that combined therapy is a promising strategy for prevention of GVHD with preservation of graft-versus-leukemia in allogenic HSCT.

Sat Feb 15 00:00:00 EST 2014

Interaction between HIV-1 Tat and DNA-PKcs modulates HIV transcription and class switch recombination.

International journal of biological sciences

Zhang SM,Zhang H,Yang TY,Ying TY,Yang PX,Liu XD,Tang SJ,Zhou PK

Published figure using IgM monoclonal antibody (Product # 11-5890-82) in Flow Cytometry

Fri Jun 26 00:00:00 EDT 2015

Control of Toll-like receptor-mediated T cell-independent type 1 antibody responses by the inducible nuclear protein IκB-ζ.

The Journal of biological chemistry

Hanihara-Tatsuzawa F,Miura H,Kobayashi S,Isagawa T,Okuma A,Manabe I,MaruYama T

11-5890 was used in Flow cytometry/Cell sorting to indicate that IκB-ζ regulates TLR-mediated class switch recombination by inducing activation-induced cytidine deaminase.

Fri Nov 07 00:00:00 EST 2014

The histone methyltransferase MMSET regulates class switch recombination.

Journal of immunology (Baltimore, Md. : 1950)

Pei H,Wu X,Liu T,Yu K,Jelinek DF,Lou Z

11-5890 was used in Flow cytometry/Cell sorting to suggest that defective CSR caused by MMSET deficiency could be a cause of Ab deficiency in WHS patients.

Tue Jan 15 00:00:00 EST 2013

The histone methyltransferase MMSET regulates class switch recombination.

Journal of immunology (Baltimore, Md. : 1950)

Pei H,Wu X,Liu T,Yu K,Jelinek DF,Lou Z

11-5890 was used in Flow cytometry/Cell sorting to suggest that defective CSR caused by MMSET deficiency could be a cause of Ab deficiency in WHS patients.

Tue Jan 15 00:00:00 EST 2013

Thymic stromal lymphopoietin (TSLP)-induced polyclonal B-cell activation and autoimmunity are mediated by CD4+ T cells and IL-4.

International immunology

Iseki M,Omori-Miyake M,Xu W,Sun X,Takaki S,Rawlings DJ,Ziegler SF

11-5890 was used in Flow cytometry/Cell sorting to demonstrate that thymic stromal lymphopoietin levels are an important factor in B-cell homeostasis and humoral autoimmunity.

Thu Mar 01 00:00:00 EST 2012

Bioinformatics

Gene Aliases: AI326478; Igh-6; Igh-M; Igh6; Igm; muH

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Entrez Gene ID: (Mouse) 16019

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Function(s)

immunoglobulin

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IgM Monoclonal Antibody (eB121-15F9), FITC, eBioscience™

IgM Antibody (11-5890-82) in Flow

Product Details

11-5890-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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