Invitrogen

MHC Class I (H-2Kb) Monoclonal Antibody (AF6-88.5.5.3), PE, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

11 Published Figures

28 References

View all (18) MHC Class I (H-2Kb) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

MHC Class I (H-2Kb) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 13

MHC Class I (H-2Kb) Antibody (12-5958-82) in Flow

C57BL/6 (left) or BALB/c (right) mouse splenocytes were stained with 0.125 µg of Mouse IgG2a kappa Isotype Control, PE (Product # 12-4724-82) (blue histogram) or 0.125 µg of MHC Class I (H-2Kb), PE (purple histogram). Total viable cells were used for analysis.

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Product Details

12-5958-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 28 publications 28 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

AF6-88.5.5.3

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10598797

Product Specific Information

Description: This AF6-88.5.5.3 monoclonal antibody reacts with the H-2Kb MHC class I alloantigen. H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8. Reactivity to other haplotypes (e.g. d, f, j, k, p, q, r, s, u, and v) has not been observed.

Applications Reported: This AF6-88.5.5.3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This AF6-88.5.5.3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

High-Dose Acetaminophen with N-acetylcysteine Rescue Inhibits M2 Polarization of Tumor-Associated Macrophages.

Cancers

Bryan A,Pingali P,Joslyn M,Li H,Bernas T,Koblinski J,Landry J,Lee WS,Patel B,Neuwelt A

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 12-5958-82) in Flow Cytometry

Thu Sep 28 00:00:00 EDT 2023

CD8α+ dendritic cells potentiate antitumor and immune activities against murine ovarian cancers.

Scientific reports

Lee SW,Lee H,Lee KW,Kim MJ,Kang SW,Lee YJ,Kim H,Kim YM

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 12-5958-82) in Flow Cytometry

Tue Jan 03 00:00:00 EST 2023

Senescence Rewires Microenvironment Sensing to Facilitate Antitumor Immunity.

Cancer discovery

Chen HA,Ho YJ,Mezzadra R,Adrover JM,Smolkin R,Zhu C,Woess K,Bernstein N,Schmitt G,Fong L,Luan W,Wuest A,Tian S,Li X,Broderick C,Hendrickson RC,Egeblad M,Chen Z,Alonso-Curbelo D,Lowe SW

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 12-5958-82) in Flow Cytometry

Mon Feb 06 00:00:00 EST 2023

CD4⁺ CTLs Act as a Key Effector Population for Allograft Rejection of MSCs in a Donor MHC-II Dependent Manner in Injured Liver.

Aging and disease

Shen S,Li Y,Jin M,Fan D,Pan R,Lin A,Chen Y,Xiang L,Zhao RC,Shao J

12-5958-82 was used in Flow cytometry/Cell sorting to reveal a novel mechanism of MSC allograft rejection mediated by CD4+ CTLs in injured liver, which provided new strategies for improving clinical performance of allo-MSCs in benefiting hepatic injury repair.

Thu Dec 01 00:00:00 EST 2022

Specific cannabinoids revive adaptive immunity by reversing immune evasion mechanisms in metastatic tumours.

Frontiers in immunology

Dada S,Ellis SLS,Wood C,Nohara LL,Dreier C,Garcia NH,Saranchova I,Munro L,Pfeifer CG,Eyford BA,Kari S,Garrovillas E,Caspani G,Al Haddad E,Gray PW,Morova T,Lack NA,Andersen RJ,Tjoelker L,Jefferies WA

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 12-5958-82) in Flow Cytometry

Mon Mar 20 00:00:00 EDT 2023

Chrysin enhances antitumour immunity response through the IL-12-STAT4 signal pathway in the B16F10 melanoma mouse model.

Scandinavian journal of immunology

Lu R,Wang S,Jiang S,Li C,Wang Y,Li L,Wang Y,Ma G,Qiao H,Leng Z,Niu J,Tian Z,Wang B

12-5958-82 was used in Flow cytometry/Cell sorting to indicate that CHR can be used as a potential adjuvant to enhance antigen immunogenicity, inhibit B16F10 tumour growth in mice and improve tumour immune response.

Mon Aug 01 00:00:00 EDT 2022

Mesenchymal stem cell exosome-derived miR-223 alleviates acute graft-versus-host disease via reducing the migration of donor T cells.

Stem cell research & therapy

Liu W,Zhou N,Liu Y,Zhang W,Li X,Wang Y,Zheng R,Zhang Y

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 12-5958-82) in Flow Cytometry

Fri Feb 26 00:00:00 EST 2021

Intravenous injection of the oncolytic virus M1 awakens antitumor T cells and overcomes resistance to checkpoint blockade.

Cell death & disease

Liu Y,Cai J,Liu W,Lin Y,Guo L,Liu X,Qin Z,Xu C,Zhang Y,Su X,Deng K,Yan G,Liang J

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 12-5958-82) in Flow Cytometry

Sat Dec 12 00:00:00 EST 2020

Bendamustine with total body irradiation conditioning yields tolerant T-cells while preserving T-cell-dependent graft-versus-leukemia.

Oncoimmunology

Stokes J,Hoffman EA,Molina MS,Kummet N,Simpson RJ,Zeng Y,Katsanis E

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 12-5958-82) in Flow Cytometry

Wed Jul 14 00:00:00 EDT 2021

Senolytics prevent mt-DNA-induced inflammation and promote the survival of aged organs following transplantation.

Nature communications

Iske J,Seyda M,Heinbokel T,Maenosono R,Minami K,Nian Y,Quante M,Falk CS,Azuma H,Martin F,Passos JF,Niemann CU,Tchkonia T,Kirkland JL,Elkhal A,Tullius SG

12-5958 was used in Flow cytometry/Cell sorting to show that cell-free mitochondrial DNA (cf-mt-DNA) released by senescent cells accumulates with aging and augments immunogenicity.

Thu Aug 27 00:00:00 EDT 2020

CXCL14 suppresses human papillomavirus-associated head and neck cancer through antigen-specific CD8⁺ T-cell responses by upregulating MHC-I expression.

Oncogene

Westrich JA,Vermeer DW,Silva A,Bonney S,Berger JN,Cicchini L,Greer RO,Song JI,Raben D,Slansky JE,Lee JH,Spanos WC,Pyeon D

12-5958-82 was used in Flow Cytometry to show that the CXCL14-mediated antitumor CD8+ T-cell responses require antigen specificity.

Fri Nov 01 00:00:00 EDT 2019

CXCL14 suppresses human papillomavirus-associated head and neck cancer through antigen-specific CD8⁺ T-cell responses by upregulating MHC-I expression.

Oncogene

Westrich JA,Vermeer DW,Silva A,Bonney S,Berger JN,Cicchini L,Greer RO,Song JI,Raben D,Slansky JE,Lee JH,Spanos WC,Pyeon D

12-5958-82 was used in Flow Cytometry to show that the CXCL14-mediated antitumor CD8+ T-cell responses require antigen specificity.

Fri Nov 01 00:00:00 EDT 2019

Platelet-derived β2m regulates age related monocyte/macrophage functions.

Aging

Hilt ZT,Ture SK,Mohan A,Arne A,Morrell CN

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 12-5958-82) in Flow Cytometry

Wed Dec 18 00:00:00 EST 2019

Persistence of Integrase-Deficient Lentiviral Vectors Correlates with the Induction of STING-Independent CD8⁺ T Cell Responses.

Cell reports

Cousin C,Oberkampf M,Felix T,Rosenbaum P,Weil R,Fabrega S,Morante V,Negri D,Cara A,Dadaglio G,Leclerc C

12-5958 was used in Flow cytometry/Cell sorting to demonstrate that integrase-deficient lentiviral vectors can trigger persistent adaptive response by the mediation of a weak and transient innate response alongside the persistence of the vector in tissues.

Tue Jan 29 00:00:00 EST 2019

Downregulation of Membrane Trafficking Proteins and Lactate Conditioning Determine Loss of Dendritic Cell Function in Lung Cancer.

Cancer research

Caronni N,Simoncello F,Stafetta F,Guarnaccia C,Ruiz-Moreno JS,Opitz B,Galli T,Proux-Gillardeaux V,Benvenuti F

12-5958 was used in Flow cytometry/Cell sorting to investigate the origin of dendritic cell dysfunction in a model of lung cancer and identify intrinsic mechanisms and extrinsic factors determining suppression.

Sun Apr 01 00:00:00 EDT 2018

Oncolysis with DTT-205 and DTT-304 generates immunological memory in cured animals.

Cell death & disease

Zhou H,Mondragón L,Xie W,Mauseth B,Leduc M,Sauvat A,Gomes-da-Silva LC,Forveille S,Iribarren K,Souquere S,Bezu L,Liu P,Zhao L,Zitvogel L,Sveinbjørnsson B,Eksteen JJ,Rekdal Ø,Kepp O,Kroemer G

12-5958 was used in Flow cytometry to show that DTT-205 and DTT-304 both have the capacity to induce immunotherapeutic oncolysis.

Tue Oct 23 00:00:00 EDT 2018

Proteomics Reveals Scope of Mycolactone-mediated Sec61 Blockade and Distinctive Stress Signature.

Molecular & cellular proteomics : MCP

Morel JD,Paatero AO,Wei J,Yewdell JW,Guenin-Macé L,Van Haver D,Impens F,Pietrosemoli N,Paavilainen VO,Demangel C

12-5958 was used in Flow cytometry/Cell sorting to use quantitative proteomics to delineate the direct and indirect effects of mycolactone-mediated Sec61 blockade in living cells.

Sat Sep 01 00:00:00 EDT 2018

Sec61 blockade by mycolactone inhibits antigen cross-presentation independently of endosome-to-cytosol export.

Proceedings of the National Academy of Sciences of the United States of America

Grotzke JE,Kozik P,Morel JD,Impens F,Pietrosemoli N,Cresswell P,Amigorena S,Demangel C

12-5958 was used in Flow cytometry/Cell sorting to analyse Sec61's contribution to antigen cross-presentation, ER-associated degradation, and transport of internalised antigens into the cytosol using a newly identified Sec61 blocker.

Tue Jul 18 00:00:00 EDT 2017

Deletion of Wiskott-Aldrich syndrome protein triggers Rac2 activity and increased cross-presentation by dendritic cells.

Nature communications

Baptista MA,Keszei M,Oliveira M,Sunahara KK,Andersson J,Dahlberg CI,Worth AJ,Liedén A,Kuo IC,Wallin RP,Snapper SB,Eidsmo L,Scheynius A,Karlsson MC,Bouma G,Burns SO,Forsell MN,Thrasher AJ,Nylén S,Westerberg LS

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 12-5958-82) in Flow Cytometry

Mon Jul 18 00:00:00 EDT 2016

CD4+ T Cells Are as Protective as CD8+ T Cells against Rickettsia typhi Infection by Activating Macrophage Bactericidal Activity.

PLoS neglected tropical diseases

Moderzynski K,Papp S,Rauch J,Heine L,Kuehl S,Richardt U,Fleischer B,Osterloh A

12-5958 was used in Flow cytometry/Cell sorting to demonstrate that CD4+ T cells are as protective as CD8+ T cells against R. typhi, provided that CD4+ TH1 effector cells are present in time to support bactericidal activity of phagocytes via the release of IFNγ and other factors.

Tue Nov 01 00:00:00 EDT 2016

Recurrence of melanoma following T cell treatment: continued antigen expression in a tumor that evades T cell recruitment.

Molecular therapy : the journal of the American Society of Gene Therapy

Straetemans T,Berrevoets C,Coccoris M,Treffers-Westerlaken E,Wijers R,Cole DK,Dardalhon V,Sewell AK,Taylor N,Verweij J,Debets R

12-5958 was used in Flow cytometry/Cell sorting to support the concept to target the tumor milieu to aid T cell therapy in limiting tumor relapse.

Sun Feb 01 00:00:00 EST 2015

Anti-angiogenic efficacy of 5'-triphosphate siRNA combining VEGF silencing and RIG-I activation in NSCLCs.

Oncotarget

Yuan D,Xia M,Meng G,Xu C,Song Y,Wei J

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 12-5958-82) in Flow Cytometry

Tue Oct 06 00:00:00 EDT 2015

Favorable alteration of tumor microenvironment by immunomodulatory cytokines for efficient T-cell therapy in solid tumors.

PloS one

Tähtinen S,Kaikkonen S,Merisalo-Soikkeli M,Grönberg-Vähä-Koskela S,Kanerva A,Parviainen S,Vähä-Koskela M,Hemminki A

12-5958 was used in Flow cytometry/Cell sorting to alter the tumor microenvironment using immunomodulatory cytokines for efficient T-cell therapy in solid tumors.

Thu Apr 28 00:00:00 EDT 2016

Microfluidic squeezing for intracellular antigen loading in polyclonal B-cells as cellular vaccines.

Scientific reports

Szeto GL,Van Egeren D,Worku H,Sharei A,Alejandro B,Park C,Frew K,Brefo M,Mao S,Heimann M,Langer R,Jensen K,Irvine DJ

12-5958 was used in Flow cytometry/Cell sorting to demonstrate proof-of-concept for mechano-poration as an enabling technology for loading B-cells with antigens to prime specific CD8(+)T-cells.

Fri May 22 00:00:00 EDT 2015

c-Rel is an essential transcription factor for the development of acute graft-versus-host disease in mice.

European journal of immunology

Yu Y,Wang D,Kaosaard K,Liu C,Fu J,Haarberg K,Anasetti C,Beg AA,Yu XZ

12-5958 was used in Flow cytometry/Cell sorting to investigate the role of c-Rel in transplant tolerance and graft-versus-host disease (GVHD), showing that it is an essential transcription factor for the development of acute GVHD.

Sun Sep 01 00:00:00 EDT 2013

Thymic damage, impaired negative selection, and development of chronic graft-versus-host disease caused by donor CD4+ and CD8+ T cells.

Journal of immunology (Baltimore, Md. : 1950)

Wu T,Young JS,Johnston H,Ni X,Deng R,Racine J,Wang M,Wang A,Todorov I,Wang J,Zeng D

12-5958 was used in Flow cytometry/Cell sorting to evaluate the contribution of T cells to the induction of chronic graft-versus-host disease.

Mon Jul 01 00:00:00 EDT 2013

The MSHA strain of Pseudomonas aeruginosa activated TLR pathway and enhanced HIV-1 DNA vaccine immunoreactivity.

PloS one

Hou J,Liu Y,Liu Y,Shao Y

12-5958 was used in Flow cytometry/Cell sorting to demonstrate the utility of mannose-sensitive hemagglutination pilus Pseudomonas aeruginosa as an adjuvant to a DNA vaccine.

Wed Apr 03 00:00:00 EDT 2013

Prevention of GVHD while sparing GVL effect by targeting Th1 and Th17 transcription factor T-bet and RORγt in mice.

Blood

Yu Y,Wang D,Liu C,Kaosaard K,Semple K,Anasetti C,Yu XZ

12-5958 was used in Flow cytometry/Cell sorting to indicate that GVHD can be prevented by targeting Th1 and Th17 transcription factors without offsetting GVL activity.

Thu Nov 03 00:00:00 EDT 2011

Stress-regulated targeting of the NKG2D ligand Mult1 by a membrane-associated RING-CH family E3 ligase.

Journal of immunology (Baltimore, Md. : 1950)

Nice TJ,Deng W,Coscoy L,Raulet DH

12-5958 was used in Flow cytometry/Cell sorting to investigate the regulation of cell surface expression of the NKG2D ligand, Mult1.

Mon Nov 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: class I major histocompatibility antigen H-2Kd; H-2 class I histocompatibility antigen, K-B alpha chain; H-2 class I histocompatibility antigen, K-D alpha chain; H-2 class I histocompatibility antigen, K-K alpha chain; H-2 class I histocompatibility antigen, K-Q alpha chain; H-2 class I histocompatibility antigen, K-W28 alpha chain; H-2K(B); H-2K(K); H-2K(Q); MHC class I antigen; MHC class I H2-K-b-alpha-2 cell surface glycoprotein; MHC class I heavy chain H2-K; MHC H2-K transplantation antigen

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Gene Aliases: H-2K; H-2K(d); H2-K; H2-K1; K-f

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UniProt ID: (Mouse) P01901

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Entrez Gene ID: (Mouse) 14972

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Function(s)

receptor binding protein binding beta-2-microglobulin binding peptide binding peptide antigen binding T cell receptor binding poly(A) RNA binding TAP binding protein heterodimerization activity major histocompatibility complex protein

Process(es)

positive regulation of T cell mediated cytotoxicity immune system process antigen processing and presentation of peptide antigen via MHC class I antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent immune response antigen processing and presentation antigen processing and presentation of exogenous peptide antigen via MHC class I defense response to bacterium inner ear development

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MHC Class I (H-2Kb) Monoclonal Antibody (AF6-88.5.5.3), PE, eBioscience™

MHC Class I (H-2Kb) Antibody (12-5958-82) in Flow

Product Details

12-5958-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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