Invitrogen

MHC Class I (H-2Kb) Monoclonal Antibody (AF6-88.5.5.3), Biotin, eBioscience™

MHC Class I (H-2Kb) Antibody in Flow Cytometry (Flow)

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MHC Class I (H-2Kb) Antibody (13-5958-82) in Flow

Staining of C57Bl/6 splenocytes with 0.06 µg of Mouse IgG2a K Isotype Control Biotin (Product # 13-4724-85) (left) or 0.06 µg of Anti-Mouse MHC Class I (H-2Kb) Biotin (right) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

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Product Details

13-5958-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.06 µg/test

View 16 publications 16 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

AF6-88.5.5.3

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1518760

Product Specific Information

Description: This AF6-88.5.5.3 monoclonal antibody reacts with the H-2Kb MHC class I alloantigen. H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8. Reactivity to other haplotypes (e.g. d, f, j, k, p, q, r, s, u, and v) has not been observed.

Applications Reported: This AF6-88.5.5.3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This AF6-88.5.5.3 antibody has been tested by flow cytometric analysis on C57Bl/6 splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

High-Dose Acetaminophen with N-acetylcysteine Rescue Inhibits M2 Polarization of Tumor-Associated Macrophages.

Cancers

Bryan A,Pingali P,Joslyn M,Li H,Bernas T,Koblinski J,Landry J,Lee WS,Patel B,Neuwelt A

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 13-5958-82) in Flow Cytometry

Thu Sep 28 00:00:00 EDT 2023

CD8α+ dendritic cells potentiate antitumor and immune activities against murine ovarian cancers.

Scientific reports

Lee SW,Lee H,Lee KW,Kim MJ,Kang SW,Lee YJ,Kim H,Kim YM

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 13-5958-82) in Flow Cytometry

Tue Jan 03 00:00:00 EST 2023

Senescence Rewires Microenvironment Sensing to Facilitate Antitumor Immunity.

Cancer discovery

Chen HA,Ho YJ,Mezzadra R,Adrover JM,Smolkin R,Zhu C,Woess K,Bernstein N,Schmitt G,Fong L,Luan W,Wuest A,Tian S,Li X,Broderick C,Hendrickson RC,Egeblad M,Chen Z,Alonso-Curbelo D,Lowe SW

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 13-5958-82) in Flow Cytometry

Mon Feb 06 00:00:00 EST 2023

Specific cannabinoids revive adaptive immunity by reversing immune evasion mechanisms in metastatic tumours.

Frontiers in immunology

Dada S,Ellis SLS,Wood C,Nohara LL,Dreier C,Garcia NH,Saranchova I,Munro L,Pfeifer CG,Eyford BA,Kari S,Garrovillas E,Caspani G,Al Haddad E,Gray PW,Morova T,Lack NA,Andersen RJ,Tjoelker L,Jefferies WA

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 13-5958-82) in Flow Cytometry

Mon Mar 20 00:00:00 EDT 2023

Mesenchymal stem cell exosome-derived miR-223 alleviates acute graft-versus-host disease via reducing the migration of donor T cells.

Stem cell research & therapy

Liu W,Zhou N,Liu Y,Zhang W,Li X,Wang Y,Zheng R,Zhang Y

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 13-5958-82) in Flow Cytometry

Fri Feb 26 00:00:00 EST 2021

Intravenous injection of the oncolytic virus M1 awakens antitumor T cells and overcomes resistance to checkpoint blockade.

Cell death & disease

Liu Y,Cai J,Liu W,Lin Y,Guo L,Liu X,Qin Z,Xu C,Zhang Y,Su X,Deng K,Yan G,Liang J

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 13-5958-82) in Flow Cytometry

Sat Dec 12 00:00:00 EST 2020

Bendamustine with total body irradiation conditioning yields tolerant T-cells while preserving T-cell-dependent graft-versus-leukemia.

Oncoimmunology

Stokes J,Hoffman EA,Molina MS,Kummet N,Simpson RJ,Zeng Y,Katsanis E

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 13-5958-82) in Flow Cytometry

Wed Jul 14 00:00:00 EDT 2021

CXCL14 suppresses human papillomavirus-associated head and neck cancer through antigen-specific CD8⁺ T-cell responses by upregulating MHC-I expression.

Oncogene

Westrich JA,Vermeer DW,Silva A,Bonney S,Berger JN,Cicchini L,Greer RO,Song JI,Raben D,Slansky JE,Lee JH,Spanos WC,Pyeon D

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 13-5958-82) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2019

Platelet-derived β2m regulates age related monocyte/macrophage functions.

Aging

Hilt ZT,Ture SK,Mohan A,Arne A,Morrell CN

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 13-5958-82) in Flow Cytometry

Wed Dec 18 00:00:00 EST 2019

Sec61 blockade by mycolactone inhibits antigen cross-presentation independently of endosome-to-cytosol export.

Proceedings of the National Academy of Sciences of the United States of America

Grotzke JE,Kozik P,Morel JD,Impens F,Pietrosemoli N,Cresswell P,Amigorena S,Demangel C

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 13-5958-82) in Flow Cytometry

Tue Jul 18 00:00:00 EDT 2017

G-CSF-Induced Suppressor IL-10+ Neutrophils Promote Regulatory T Cells That Inhibit Graft-Versus-Host Disease in a Long-Lasting and Specific Way.

Journal of immunology (Baltimore, Md. : 1950)

Perobelli SM,Mercadante AC,Galvani RG,Gonçalves-Silva T,Alves AP,Pereira-Neves A,Benchimol M,Nóbrega A,Bonomo A

13-5958 was used in Flow cytometry/Cell sorting to study the role of incoming IL-10+ neutrophils from G-CSF-treated donor spleen in inhibiting acute graft-versus-host disease.

Tue Nov 01 00:00:00 EDT 2016

Deletion of Wiskott-Aldrich syndrome protein triggers Rac2 activity and increased cross-presentation by dendritic cells.

Nature communications

Baptista MA,Keszei M,Oliveira M,Sunahara KK,Andersson J,Dahlberg CI,Worth AJ,Liedén A,Kuo IC,Wallin RP,Snapper SB,Eidsmo L,Scheynius A,Karlsson MC,Bouma G,Burns SO,Forsell MN,Thrasher AJ,Nylén S,Westerberg LS

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 13-5958-82) in Flow Cytometry

Mon Jul 18 00:00:00 EDT 2016

Anti-angiogenic efficacy of 5'-triphosphate siRNA combining VEGF silencing and RIG-I activation in NSCLCs.

Oncotarget

Yuan D,Xia M,Meng G,Xu C,Song Y,Wei J

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 13-5958-82) in Flow Cytometry

Tue Oct 06 00:00:00 EDT 2015

c-Rel is an essential transcription factor for the development of acute graft-versus-host disease in mice.

European journal of immunology

Yu Y,Wang D,Kaosaard K,Liu C,Fu J,Haarberg K,Anasetti C,Beg AA,Yu XZ

13-5958 was used in Flow cytometry/Cell sorting to investigate the role of c-Rel in transplant tolerance and graft-versus-host disease (GVHD), showing that it is an essential transcription factor for the development of acute GVHD.

Sun Sep 01 00:00:00 EDT 2013

Prevention of GVHD while sparing GVL effect by targeting Th1 and Th17 transcription factor T-bet and RORγt in mice.

Blood

Yu Y,Wang D,Liu C,Kaosaard K,Semple K,Anasetti C,Yu XZ

13-5958 was used in Flow cytometry/Cell sorting to indicate that GVHD can be prevented by targeting Th1 and Th17 transcription factors without offsetting GVL activity.

Thu Nov 03 00:00:00 EDT 2011

Tumor-derived exosomes confer antigen-specific immunosuppression in a murine delayed-type hypersensitivity model.

PloS one

Yang C,Kim SH,Bianco NR,Robbins PD

13-5958 was used in Flow cytometry/Cell sorting to study the role of tumour-derived exosomes in inducing tumour antigen-specific immunosuppression.

Tue Dec 27 00:00:00 EST 2011

Bioinformatics

Protein Aliases: class I major histocompatibility antigen H-2Kd; H-2 class I histocompatibility antigen, K-B alpha chain; H-2 class I histocompatibility antigen, K-D alpha chain; H-2 class I histocompatibility antigen, K-K alpha chain; H-2 class I histocompatibility antigen, K-Q alpha chain; H-2 class I histocompatibility antigen, K-W28 alpha chain; H-2K(B); H-2K(K); H-2K(Q); MHC class I antigen; MHC class I H2-K-b-alpha-2 cell surface glycoprotein; MHC class I heavy chain H2-K; MHC H2-K transplantation antigen

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Gene Aliases: H-2K; H-2K(d); H2-K; H2-K1; K-f

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UniProt ID: (Mouse) P01901

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Entrez Gene ID: (Mouse) 14972

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Function(s)

receptor binding protein binding beta-2-microglobulin binding peptide binding peptide antigen binding T cell receptor binding poly(A) RNA binding TAP binding protein heterodimerization activity major histocompatibility complex protein

Process(es)

positive regulation of T cell mediated cytotoxicity immune system process antigen processing and presentation of peptide antigen via MHC class I antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent immune response antigen processing and presentation antigen processing and presentation of exogenous peptide antigen via MHC class I defense response to bacterium inner ear development

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

MHC Class I (H-2Kb) Monoclonal Antibody (AF6-88.5.5.3), Biotin, eBioscience™

MHC Class I (H-2Kb) Antibody (13-5958-82) in Flow

Product Details

13-5958-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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