Invitrogen

IL-12/IL-23 p40 Monoclonal Antibody (C17.8), PE, eBioscience™

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Antibody Testing Data (1)
Published Figures (4)

IL-12/IL-23 p40 Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 5

IL-12/IL-23 p40 Antibody (12-7123-82) in Flow

Mouse thioglycolate-elicited peritoneal exudate cells were stimulated overnight with LPS and IFN gamma in the presence of Brefeldin A. Cells were then fixed, permeabilized and stained with 0.125 µg of Rat IgG2a K Isotype Control PE (left) or 0.125 µg of Anti-Mouse IL-12/IL-23 p40 PE (right). Total viable cells, as determined by Fixable Viability Dye eFluor® 780 (Product # 65-0865-14), were used for analysis.

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Product Details

12-7123-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 23 publications 23 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

C17.8

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466185

Product Specific Information

Description: The C17.8 antibody reacts with the p40 subunit of IL-12 and IL-23. The C17.8 antibody has been found to neutralize IL-12 and IL-23 bioactivity. For specific neutralization of IL-23 only, please see clone G23-8 (anti-mouse IL-23 p19). IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and is secreted by activated monocytes, macrophages, and dendritic cells. IL-23 is a heterodimer composed of the disulfide-linked p19 protein and p40 protein, expressed by activated dendritic cells.

Applications Reported:The C17.8 antibody has been reported for use in IP, Western, ELISA, neutralization, and intracellular staining for flow cytometric analysis.

Applications Tested: Has been tested by intracellular flow cytometric analysis of stimulated mouse cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-12 is an interleukin produced by dendritic cells, neutrophils, macrophages and human lymphoblastoid cells as a result of antigenic stimulation. IL-12 is a heterodimeric cytokine encoded by IL-12A (p35) and IL-12B (p40). IL-23 is a heterodimeric cytokine that shares a subunit with IL-12 (IL-12B) along with an IL-23A (IL-23p19) subunit. Functionally, IL-12 increases the expression of vascular endothelial adhesion molecule-1 on the endothelium, directs leukocytes towards tumors and facilitates the ischemic-hemorrhagic necrosis of the tumor tissue. In comparison, IL-23 plays an important role in stimulating memory T-cells, and is required for the induction, expansion, maintenance and downstream effector functions of Th17 cells, which play a vital role in upregulating neutrophil chemokines and various pro-inflammatory cytokines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

An optical system for noninvasive microscopy of psoriatic mice in vivo.

Journal of biophotonics

Lu Y,Zhu Y,Zhao X,Pan M,He H

12-7123-82 was used in In vivo experimentsto suggest IL-23 recruits CD4+ cells to release IL-17 in blood that further leaks out in the psoriatic skin area.

Sat Apr 01 00:00:00 EDT 2023

Dendritic cell Piezo1 directs the differentiation of T_H1 and T_reg cells in cancer.

eLife

Wang Y,Yang H,Jia A,Wang Y,Yang Q,Dong Y,Hou Y,Cao Y,Dong L,Bi Y,Liu G

12-7123-82 was used in Flow cytometry/Cell sorting to demonstrate the role of the dendritic cells-based mechanical regulation of immunopathology in directing T cell lineage commitment in tumor microenvironments.

Mon Aug 22 00:00:00 EDT 2022

Tet2 deficiency drives liver microbiome dysbiosis triggering Tc1 cell autoimmune hepatitis.

Cell host & microbe

Pandey SP,Bender MJ,McPherson AC,Phelps CM,Sanchez LM,Rana M,Hedden L,Sangani KA,Chen L,Shapira JH,Siller M,Goel C,Verdú EF,Jabri B,Chang A,Chandran UR,Mullett SJ,Wendell SG,Singhi AD,Tilstra JS,Pierre JF,Arteel GE,Hinterleitner R,Meisel M

12-7123-82 was used in Flow cytometry/Cell sorting to demonstrate that I3A is required for L. reuteri-induced Tc1 cell differentiation in vitro and AIH-like pathology in vivo, both of which are restrained by Tet2 within CD8 T cells.

Wed Jul 13 00:00:00 EDT 2022

The deubiquitinase OTUB1 augments NF-κB-dependent immune responses in dendritic cells in infection and inflammation by stabilizing UBC13.

Cellular & molecular immunology

Mulas F,Wang X,Song S,Nishanth G,Yi W,Brunn A,Larsen PK,Isermann B,Kalinke U,Barragan A,Naumann M,Deckert M,Schlüter D

12-7123 was used in Flow cytometry/Cell sorting to reveal that the deubiquitinase OTU domain, ubiquitin aldehyde binding 1 (OTUB1) is upregulated in DCs upon murine Toxoplasma gondii infection and lipopolysaccharide challenge.

Tue Jun 01 00:00:00 EDT 2021

Intratumoral expression of interleukin 23 variants using oncolytic vaccinia virus elicit potent antitumor effects on multiple tumor models via tumor microenvironment modulation.

Theranostics

Chen L,Chen H,Ye J,Ge Y,Wang H,Dai E,Ren J,Liu W,Ma C,Ju S,Guo ZS,Liu Z,Bartlett DL

12-7123-82 was used in Flow cytometry/Cell sorting to explore the use of virally-delivered interleukin 23 as an anti-cancer treatment.

Wed Aug 04 00:00:00 EDT 2021

Oncolytic vaccinia virus delivering tethered IL-12 enhances antitumor effects with improved safety.

Journal for immunotherapy of cancer

Ge Y,Wang H,Ren J,Liu W,Chen L,Chen H,Ye J,Dai E,Ma C,Ju S,Guo ZS,Liu Z,Bartlett DL

12-7123-82 was used in Flow Cytometry to test whether oncolytic vaccinia virus delivering tethered interleukin 12 could turn a "cold" tumor into a "hot" tumor while avoiding IL-12's systemic toxicity.

Sun Mar 01 00:00:00 EST 2020

Requirements for the differentiation of innate T-bet^high memory-phenotype CD4⁺ T lymphocytes under steady state.

Nature communications

Kawabe T,Yi J,Kawajiri A,Hilligan K,Fang D,Ishii N,Yamane H,Zhu J,Jankovic D,Kim KS,Trinchieri G,Sher A

Published figure using IL-12/IL-23 p40 monoclonal antibody (Product # 12-7123-82) in Flow Cytometry

Mon Jul 06 00:00:00 EDT 2020

Murine CMV induces type 1 IFN that impairs differentiation of MDSCs critical for transplantation tolerance.

Blood advances

Dangi A,Zhang L,Zhang X,Luo X

12-7123 was used in Flow cytometry/Cell sorting to provide a solid foundation for seeking effective therapies for promoting transplantation tolerance in settings of CMV infection.

Tue Mar 27 00:00:00 EDT 2018

Chloroquine modulates antitumor immune response by resetting tumor-associated macrophages toward M1 phenotype.

Nature communications

Chen D,Xie J,Fiskesund R,Dong W,Liang X,Lv J,Jin X,Liu J,Mo S,Zhang T,Cheng F,Zhou Y,Zhang H,Tang K,Ma J,Liu Y,Huang B

Wed Feb 28 00:00:00 EST 2018

Murine Norovirus Infection Induces T_H1 Inflammatory Responses to Dietary Antigens.

Cell host & microbe

Bouziat R,Biering SB,Kouame E,Sangani KA,Kang S,Ernest JD,Varma M,Brown JJ,Urbanek K,Dermody TS,Ng A,Hinterleitner R,Hwang S,Jabri B

12-7123-82 was used in Flow cytometry/Cell sorting to show that the acute CW3 strain of murine norovirus, but not the persistent CR6 strain, induces TH1 immunity to dietary antigen.

Wed Nov 14 00:00:00 EST 2018

1,25-Dihydroxyvitamin D suppresses M1 macrophages and promotes M2 differentiation at bone injury sites.

JCI insight

Wasnik S,Rundle CH,Baylink DJ,Yazdi MS,Carreon EE,Xu Y,Qin X,Lau KW,Tang X

12-7123 was used in Flow cytometry/Cell sorting to define the role of M1 macrophages at bone injury sites via the function of 1,25-Dihydroxyvitamin D in suppressing M1 but promoting M2 differentiation.

Thu Sep 06 00:00:00 EDT 2018

G-CSF-Induced Suppressor IL-10+ Neutrophils Promote Regulatory T Cells That Inhibit Graft-Versus-Host Disease in a Long-Lasting and Specific Way.

Journal of immunology (Baltimore, Md. : 1950)

Perobelli SM,Mercadante AC,Galvani RG,Gonçalves-Silva T,Alves AP,Pereira-Neves A,Benchimol M,Nóbrega A,Bonomo A

12-7123 was used in Flow cytometry/Cell sorting to study the role of incoming IL-10+ neutrophils from G-CSF-treated donor spleen in inhibiting acute graft-versus-host disease.

Tue Nov 01 00:00:00 EDT 2016

Batf3-dependent CD103+ dendritic cells are major producers of IL-12 that drive local Th1 immunity against Leishmania major infection in mice.

European journal of immunology

Martínez-López M,Iborra S,Conde-Garrosa R,Sancho D

12-7123 was used in Flow cytometry/Cell sorting to suggest that IL-12 production by Batf3-dependent dendritic cell determination is crucial for maintenance of local T helper 1 cell immunity against Leishmania major infection.

Thu Jan 01 00:00:00 EST 2015

Plant virus particles carrying tumour antigen activate TLR7 and Induce high levels of protective antibody.

PloS one

Jobsri J,Allen A,Rajagopal D,Shipton M,Kanyuka K,Lomonossoff GP,Ottensmeier C,Diebold SS,Stevenson FK,Savelyeva N

12-7123 was used in Flow cytometry/Cell sorting to assess the effectiveness of a plant viral particle conjugate vaccine against a murine B-cell malignancy.

Mon Jan 25 00:00:00 EST 2016

IL-23 from Langerhans cells is required for the development of imiquimod-induced psoriasis-like dermatitis by induction of IL-17A-producing γδ T cells.

The Journal of investigative dermatology

Yoshiki R,Kabashima K,Honda T,Nakamizo S,Sawada Y,Sugita K,Yoshioka H,Ohmori S,Malissen B,Tokura Y,Nakamura M

12-7123 was used in Flow cytometry/Cell sorting to clarify the role of Langerhans cells in psoriasis.

Tue Jul 01 00:00:00 EDT 2014

Notch2-dependent classical dendritic cells orchestrate intestinal immunity to attaching-and-effacing bacterial pathogens.

Nature immunology

Satpathy AT,Briseño CG,Lee JS,Ng D,Manieri NA,Kc W,Wu X,Thomas SR,Lee WL,Turkoz M,McDonald KG,Meredith MM,Song C,Guidos CJ,Newberry RD,Ouyang W,Murphy TL,Stappenbeck TS,Gommerman JL,Nussenzweig MC,Colonna M,Kopan R,Murphy KM

12-7123 was used in Flow cytometry/Cell sorting to elucidate the cellular source of essential IL-23 production required for the mucosal response to attaching-and-effacing bacterial infection.

Sun Sep 01 00:00:00 EDT 2013

CX(3)CR1(+) macrophages support IL-22 production by innate lymphoid cells during infection with Citrobacter rodentium.

Mucosal immunology

Manta C,Heupel E,Radulovic K,Rossini V,Garbi N,Riedel CU,Niess JH

12-7123 was used in Flow cytometry/Cell sorting to show that macrophages support IL-22 production by innate lymphoid cells during infection with Citrobacter rodentum.

Tue Jan 01 00:00:00 EST 2013

Hematopoietic miR155 deficiency enhances atherosclerosis and decreases plaque stability in hyperlipidemic mice.

PloS one

Donners MM,Wolfs IM,Stöger LJ,van der Vorst EP,Pöttgens CC,Heymans S,Schroen B,Gijbels MJ,de Winther MP

12-7123 was used in Flow cytometry/Cell sorting to investigate the role of miR155 in atherosclerosis in vivo, showing that miR155 deficiency enhances atherosclerosis and decreases plaque stability.

Thu Sep 13 00:00:00 EDT 2012

Polysaccharide krestin is a novel TLR2 agonist that mediates inhibition of tumor growth via stimulation of CD8 T cells and NK cells.

Clinical cancer research : an official journal of the American Association for Cancer Research

Lu H,Yang Y,Gad E,Wenner CA,Chang A,Larson ER,Dang Y,Martzen M,Standish LJ,Disis ML

12-7123 was used in Flow cytometry/Cell sorting to demonstrate that polysaccharide krestin is a specific TLR2 agonist and has potent antitumor effects.

Sat Jan 01 00:00:00 EST 2011

Mechanisms by which chronic ethanol feeding limits the ability of dendritic cells to stimulate T-cell proliferation.

Alcoholism, clinical and experimental research

Fan J,Edsen-Moore MR,Turner LE,Cook RT,Legge KL,Waldschmidt TJ,Schlueter AJ

12-7123 was used in Flow cytometry/Cell sorting to assess the functional capability of ethanol-exposed dendritic cells, using a murine model of chronic alcohol abuse.

Sat Jan 01 00:00:00 EST 2011

Enhancement of immunogenicity of a therapeutic cervical cancer DNA-based vaccine by co-application of sequence-optimized genetic adjuvants.

International journal of cancer

Ohlschläger P,Quetting M,Alvarez G,Dürst M,Gissmann L,Kaufmann AM

12-7123 was used in Flow cytometry/Cell sorting to suggest that adding adjuvant genes may be enhance synergistic immune responses by DNA immunization for the treatment of cervical cancer.

Wed Jul 01 00:00:00 EDT 2009

Distinct roles for MyD88 and Toll-like receptor 2 during Leishmania braziliensis infection in mice.

Infection and immunity

Vargas-Inchaustegui DA,Tai W,Xin L,Hogg AE,Corry DB,Soong L

12-7123 was used in Flow cytometry/Cell sorting to investigate whether TLRs are involved in L. braziliensis-mediated dendritic cell activation, showing distinct roles for MyD88 and TLR2 in infection.

Wed Jul 01 00:00:00 EDT 2009

Leishmania braziliensis infection induces dendritic cell activation, ISG15 transcription, and the generation of protective immune responses.

Journal of immunology (Baltimore, Md. : 1950)

Vargas-Inchaustegui DA,Xin L,Soong L

12-7123 was used in Flow cytometry/Cell sorting to investigate whether Leishmania braziliensis infection efficiently triggers innate responses in dendritic cells and the subsequent adaptive immune responses for parasite clearance.

Sun Jun 01 00:00:00 EDT 2008

Role of interleukin-1beta in activating the CD11c(high) CD45RB- dendritic cell subset and priming Leishmania amazonensis-specific CD4+ T cells in vitro and in vivo.

Infection and immunity

Xin L,Li Y,Soong L

12-7123 was used in Flow cytometry/Cell sorting to investigate the mechanism by which the Leishmania amazonensis parasite modulates antigen-presenting cells and disrupts the immune system.

Mon Oct 01 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: CLMF p35; CLMF p40; Cytotoxic lymphocyte maturation factor 35 kDa subunit; Cytotoxic lymphocyte maturation factor 40 kDa subunit; il 12; IL 12/IL 23p40; il 23; IL 23 p40; IL-12 subunit p35; IL-12 subunit p40; IL-12A; IL-12B; IL-23 subunit alpha; IL-23-A; IL-23p19; IL12 p40; IL23; il23; IL23 p41; ILN; Interleukin; Interleukin 12; Interleukin-12 subunit alpha; Interleukin-12 subunit beta; Interleukin-23 subunit alpha; Interleukin-23 subunit p19; Interleukin12; RP23-388G23.1

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Gene Aliases: Il-12a; Il-12b; IL-12p35; Il-12p40; IL-23; Il12a; Il12b; Il12p40; Il23a; Ll12a; p19; p35; p40

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UniProt ID: (Mouse) P43431, (Mouse) P43432, (Mouse) Q9EQ14

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Entrez Gene ID: (Mouse) 16159, (Mouse) 16160, (Mouse) 83430

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Function(s)

cytokine activity protein binding interleukin-23 receptor binding cytokine interleukin superfamily intercellular signal molecule transmembrane signal receptor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-12/IL-23 p40 Monoclonal Antibody (C17.8), PE, eBioscience™

IL-12/IL-23 p40 Antibody (12-7123-82) in Flow

Product Details

12-7123-82

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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