Invitrogen

IL-12/IL-23 p40 Monoclonal Antibody (C17.8), eFluor™ 660, eBioscience™

5 Published Figures

7 References

View all (44) IL-12/IL-23 p40 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (5)

IL-12/IL-23 p40 Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 6

IL-12/IL-23 p40 Antibody (50-7123-82) in Flow

Mouse thioglycolate-elicited peritoneal exudate cells were stimulated overnight with LPS and IFN gamma in the presence of Brefeldin A. Cells were surface stained with Anti-Mouse F4/80 Antigen FITC (Product # 11-4801-82) followed by intracellular staining with 0.06 µg of Rat IgG2a K Isotype Control eFluor® 660 (Product # 50-4321-82) (left) or 0.06 µg of Anti-Mouse IL-12/IL-23 p40 eFluor® 660 (right) using the IC Fixation and Permeabilization Buffers (Product # 88-8823-88). Total cells were used for analysis.

IL-12/IL-23 p40 Antibody in Neutralization (Neu)

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Product Details

50-7123-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 6 publications 6 publications

Neutralization (Neu)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 660, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

C17.8

Conjugate

eFluor™ 660 eFluor™ 660 eFluor™ 660

Excitation/Emission Max

651/668 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11218493

Product Specific Information

Description: The C17.8 antibody reacts with the p40 subunit of IL-12 and IL-23. The C17.8 antibody has been found to neutralize IL-12 and IL-23 bioactivity. For specific neutralization of IL-23 only, please see clone G23-8 (anti-mouse IL-23 p19). IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and is secreted by activated monocytes, macrophages, and dendritic cells. IL-23 is a heterodimer composed of the disulfide-linked p19 protein and p40 protein, expressed by activated dendritic cells.

Applications Reported: This C17.8 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This C17.8 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-12 is an interleukin produced by dendritic cells, neutrophils, macrophages and human lymphoblastoid cells as a result of antigenic stimulation. IL-12 is a heterodimeric cytokine encoded by IL-12A (p35) and IL-12B (p40). IL-23 is a heterodimeric cytokine that shares a subunit with IL-12 (IL-12B) along with an IL-23A (IL-23p19) subunit. Functionally, IL-12 increases the expression of vascular endothelial adhesion molecule-1 on the endothelium, directs leukocytes towards tumors and facilitates the ischemic-hemorrhagic necrosis of the tumor tissue. In comparison, IL-23 plays an important role in stimulating memory T-cells, and is required for the induction, expansion, maintenance and downstream effector functions of Th17 cells, which play a vital role in upregulating neutrophil chemokines and various pro-inflammatory cytokines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

CD300a Receptor Blocking Enhances Early Clearance of Leishmania donovani From Its Mammalian Host Through Modulation of Effector Functions of Phagocytic and Antigen Experienced T Cells.

Frontiers in immunology

Singh R,Anand A,Rawat AK,Saini S,Mahapatra B,Singh NK,Mishra AK,Singh S,Singh N,Kishore D,Kumar V,Das P,Singh RK

50-7123-82 was used in Flow cytometry/Cell sorting to report that L. donovani, which causes visceral leishmaniasis in the Indian subcontinent, upregulates the expression of an immune inhibitory receptor i.e., CD300a on antigen presenting and phagocytic cells to dampen their effector functions.

Thu Feb 24 00:00:00 EST 2022

Requirements for the differentiation of innate T-bet^high memory-phenotype CD4⁺ T lymphocytes under steady state.

Nature communications

Kawabe T,Yi J,Kawajiri A,Hilligan K,Fang D,Ishii N,Yamane H,Zhu J,Jankovic D,Kim KS,Trinchieri G,Sher A

Published figure using IL-12/IL-23 p40 monoclonal antibody (Product # 50-7123-82) in Flow Cytometry

Mon Jul 06 00:00:00 EDT 2020

Influenza Vaccination Primes Human Myeloid Cell Cytokine Secretion and NK Cell Function.

Journal of immunology (Baltimore, Md. : 1950)

Wagstaffe HR,Pickering H,Houghton J,Mooney JP,Wolf AS,Prevatt N,Behrens RH,Holland MJ,Riley EM,Goodier MR

50-7123 was used in Flow cytometry/Cell sorting to study the effects of IL-15 stimulation on the active influenza vaccine and CIML NK cell functions using healthy human adults.

Sun Sep 15 00:00:00 EDT 2019

IL-15 Promotes Polyfunctional NK Cell Responses to Influenza by Boosting IL-12 Production.

Journal of immunology (Baltimore, Md. : 1950)

Wagstaffe HR,Nielsen CM,Riley EM,Goodier MR

50-7123 was used in Flow cytometry/Cell sorting to suggest that very low concentrations of IL-15 play an important role in boosting accessory cell function to support NK cell effector functions.

Sun Apr 15 00:00:00 EDT 2018

Chloroquine modulates antitumor immune response by resetting tumor-associated macrophages toward M1 phenotype.

Nature communications

Chen D,Xie J,Fiskesund R,Dong W,Liang X,Lv J,Jin X,Liu J,Mo S,Zhang T,Cheng F,Zhou Y,Zhang H,Tang K,Ma J,Liu Y,Huang B

Published figure using IL-12/IL-23 p40 monoclonal antibody (Product # 50-7123-82) in Flow Cytometry

Wed Feb 28 00:00:00 EST 2018

CD1b-autoreactive T cells recognize phospholipid antigens and contribute to antitumor immunity against a CD1b⁺ T cell lymphoma.

Oncoimmunology

Bagchi S,Li S,Wang CR

Published figure using IL-12/IL-23 p40 monoclonal antibody (Product # 50-7123-82) in Neutralization

Tue Jan 12 00:00:00 EST 2021

CX(3)CR1(+) macrophages support IL-22 production by innate lymphoid cells during infection with Citrobacter rodentium.

Mucosal immunology

Manta C,Heupel E,Radulovic K,Rossini V,Garbi N,Riedel CU,Niess JH

Published figure using IL-12/IL-23 p40 monoclonal antibody (Product # 50-7123-82) in Flow Cytometry

Tue Jan 01 00:00:00 EST 2013

Bioinformatics

Protein Aliases: CLMF p35; CLMF p40; Cytotoxic lymphocyte maturation factor 35 kDa subunit; Cytotoxic lymphocyte maturation factor 40 kDa subunit; il 12; IL 12/IL 23p40; il 23; IL 23 p40; IL-12 subunit p35; IL-12 subunit p40; IL-12A; IL-12B; IL-23 subunit alpha; IL-23-A; IL-23p19; IL12 p40; IL23; il23; IL23 p41; ILN; Interleukin; Interleukin 12; Interleukin-12 subunit alpha; Interleukin-12 subunit beta; Interleukin-23 subunit alpha; Interleukin-23 subunit p19; Interleukin12; RP23-388G23.1

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Gene Aliases: Il-12a; Il-12b; IL-12p35; Il-12p40; IL-23; Il12a; Il12b; Il12p40; Il23a; Ll12a; p19; p35; p40

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UniProt ID: (Mouse) P43431, (Mouse) P43432, (Mouse) Q9EQ14

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Entrez Gene ID: (Mouse) 16159, (Mouse) 16160, (Mouse) 83430

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Function(s)

cytokine activity protein binding interleukin-23 receptor binding cytokine interleukin superfamily intercellular signal molecule transmembrane signal receptor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-12/IL-23 p40 Monoclonal Antibody (C17.8), eFluor™ 660, eBioscience™

IL-12/IL-23 p40 Antibody (50-7123-82) in Flow

Product Details

50-7123-82

Flow Cytometry (Flow)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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