Invitrogen

IL-12/IL-23 p40 Monoclonal Antibody (C17.8), Functional Grade, eBioscience™

5 Published Figures

32 References

View all (44) IL-12/IL-23 p40 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (5)

IL-12/IL-23 p40 Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 6

IL-12/IL-23 p40 Antibody (16-7123-81) in Flow

BALB/c mouse thioglycolate-elicited peritoneal exudate cells were stimulated overnight with LPS (Product # 00-4976-03) and IFN gamma (Product # BMS326) in presence of Brefeldin A (Product # 00-4506-51) and then stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with CD11b Monoclonal Antibody, APC (Product # 17-0112-82) and 0.125 µg of Rat IgG2a kappa Isotype Control (Product # 14-4321-82) (left) or 0.125 µg of IL-12/IL-23 p40 Monoclonal Antibody, Functional Grade (ri... View More

IL-12/IL-23 p40 Antibody in Neutralization (Neu)

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Product Details

16-7123-81

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

Flow Cytometry (Flow)

Assay-Dependent

View 12 publications 12 publications

ELISA (ELISA)

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-Dependent

Neutralization (Neu)

Assay-Dependent

View 8 publications 8 publications

Functional Assay (Functional)

Assay-Dependent

View 8 publications 8 publications

T-Cell Activation (TCA)

View 1 publication 1 publication

In vitro Assay (IV)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

C17.8

Conjugate

Functional Grade Functional Grade Functional Grade

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_469232

Product Specific Information

Description: The C17.8 antibody reacts with the p40 subunit of IL-12 and IL-23. The C17.8 antibody has been found to neutralize IL-12 and IL-23 bioactivity. For specific neutralization of IL-23 only, please see clone G23-8 (anti-mouse IL-23 p19). IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and is secreted by activated monocytes, macrophages, and dendritic cells. IL-23 is a heterodimer composed of the disulfide-linked p19 protein and p40 protein, expressed by activated dendritic cells.

Applications Reported: The C17.8 antibody has been reported for use in IP, Western, ELISA, neutralization, and intracellular staining for flow cytometric analysis.

Applications Tested: The Functional Grade Purified C17.8 antibody has been tested by LAL assay to verify low endotoxin levels and has been tested for ELISA detection and in bioassay for neutralization of IL-12 p70 and IL-23 bioactivity.

The C17.8 antibody at 62.0 ng/mL has been found to inhibit by 50% the biological effects of 1 ng/mL mouse IL-12 p70 (14-8121) or mouse IL-23 (14-8231), in corresponding bioassay. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols® section.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

IL-12 is an interleukin produced by dendritic cells, neutrophils, macrophages and human lymphoblastoid cells as a result of antigenic stimulation. IL-12 is a heterodimeric cytokine encoded by IL-12A (p35) and IL-12B (p40). IL-23 is a heterodimeric cytokine that shares a subunit with IL-12 (IL-12B) along with an IL-23A (IL-23p19) subunit. Functionally, IL-12 increases the expression of vascular endothelial adhesion molecule-1 on the endothelium, directs leukocytes towards tumors and facilitates the ischemic-hemorrhagic necrosis of the tumor tissue. In comparison, IL-23 plays an important role in stimulating memory T-cells, and is required for the induction, expansion, maintenance and downstream effector functions of Th17 cells, which play a vital role in upregulating neutrophil chemokines and various pro-inflammatory cytokines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Deletion of Ppard in CD11c⁺ cells attenuates atherosclerosis in ApoE knockout mice.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Tian D,Hong H,Shang W,Ho CC,Dong J,Tian XY

16-7123-81 was used in Flow Cytometry to study the involvement of PPARδ in CD11c+ cells in atherosclerosis.

Sat Feb 01 00:00:00 EST 2020

MiR-1165-3p Suppresses Th2 Differentiation via Targeting IL-13 and PPM1A in a Mouse Model of Allergic Airway Inflammation.

Allergy, asthma & immunology research

Wang Z,Ji N,Chen Z,Sun Z,Wu C,Yu W,Hu F,Huang M,Zhang M

16-7123-38 was used in T-Cell Activation to investigate the associations between Th2 differentiation and miR-1165b-3p in asthma as well as the possible mechanisms.

Tue Sep 01 00:00:00 EDT 2020

Requirements for the differentiation of innate T-bet^high memory-phenotype CD4⁺ T lymphocytes under steady state.

Nature communications

Kawabe T,Yi J,Kawajiri A,Hilligan K,Fang D,Ishii N,Yamane H,Zhu J,Jankovic D,Kim KS,Trinchieri G,Sher A

Published figure using IL-12/IL-23 p40 monoclonal antibody (Product # 16-7123-81) in Flow Cytometry

Mon Jul 06 00:00:00 EDT 2020

Gasdermin D in peripheral myeloid cells drives neuroinflammation in experimental autoimmune encephalomyelitis.

The Journal of experimental medicine

Li S,Wu Y,Yang D,Wu C,Ma C,Liu X,Moynagh PN,Wang B,Hu G,Yang S

16-7123-81 was used in Flow Cytometry to study how GSDMD deficiency in peripheral myeloid cells impaired the infiltration of immune cells into the CNS, leading to the suppression of neuroinflammation and demyelination.

Mon Nov 04 00:00:00 EST 2019

Chloroquine modulates antitumor immune response by resetting tumor-associated macrophages toward M1 phenotype.

Nature communications

Chen D,Xie J,Fiskesund R,Dong W,Liang X,Lv J,Jin X,Liu J,Mo S,Zhang T,Cheng F,Zhou Y,Zhang H,Tang K,Ma J,Liu Y,Huang B

Published figure using IL-12/IL-23 p40 monoclonal antibody (Product # 16-7123-81) in Flow Cytometry

Wed Feb 28 00:00:00 EST 2018

α-Galactosylceramide-activated murine NK1.1(+) invariant-NKT cells in the myometrium induce miscarriages in mice.

European journal of immunology

Ichikawa T,Negishi Y,Shimizu M,Takeshita T,Takahashi H

16-7123 was used in Flow cytometry/Cell sorting to study the mechanism by which administration of IL-12 to syngeneic-mated C57BL/6 mice on gestation day 7.5 drives significant miscarriages in pregnant females.

Mon Aug 01 00:00:00 EDT 2016

Blood Stem Cell Activity Is Arrested by Th1-Mediated Injury Preventing Engraftment following Nonmyeloablative Conditioning.

Journal of immunology (Baltimore, Md. : 1950)

Müller AM,Florek M,Kohrt HE,Küpper NJ,Filatenkov A,Linderman JA,Hadeiba H,Negrin RS,Shizuru JA

16-7123 was used in Flow cytometry/Cell sorting to shed new light on the effects of CD4+ T cells on HSC biology.

Tue Nov 15 00:00:00 EST 2016

CD1b-autoreactive T cells recognize phospholipid antigens and contribute to antitumor immunity against a CD1b⁺ T cell lymphoma.

Oncoimmunology

Bagchi S,Li S,Wang CR

Tue Jan 12 00:00:00 EST 2021

CD1b-autoreactive T cells recognize phospholipid antigens and contribute to antitumor immunity against a CD1b⁺ T cell lymphoma.

Oncoimmunology

Bagchi S,Li S,Wang CR

Tue Jan 12 00:00:00 EST 2021

Plasmacytoid dendritic cells orchestrate TLR7-mediated innate and adaptive immunity for the initiation of autoimmune inflammation.

Scientific reports

Takagi H,Arimura K,Uto T,Fukaya T,Nakamura T,Choijookhuu N,Hishikawa Y,Sato K

16-7123 was used in Functional assays to investigate the role of plasmacytoid dendritic cells in autoimmunity, showing that they orchestrate TLR7-mediated immune response and initiate autoimmune inflammation.

Thu Apr 14 00:00:00 EDT 2016

Ly6C(high) monocytes control cerebral toxoplasmosis.

Journal of immunology (Baltimore, Md. : 1950)

Biswas A,Bruder D,Wolf SA,Jeron A,Mack M,Heimesaat MM,Dunay IR

16-7123 was used in Flow cytometry/Cell sorting to indicate the critical importance of recruited Ly6C monocytes upon cerebral toxoplasmosis.

Wed Apr 01 00:00:00 EDT 2015

Phagosomal Acidification Prevents Macrophage Inflammatory Cytokine Production to Malaria, and Dendritic Cells Are the Major Source at the Early Stages of Infection: IMPLICATION FOR MALARIA PROTECTIVE IMMUNITY DEVELOPMENT.

The Journal of biological chemistry

Wu X,Gowda NM,Gowda DC

16-7123 was used in Flow cytometry/Cell sorting to demonstrate that monocytes and macrophages do not produce inflammatory cytokines to malaria parasites and that DCs are the primary source.

Fri Sep 18 00:00:00 EDT 2015

The AGC kinase SGK1 regulates TH1 and TH2 differentiation downstream of the mTORC2 complex.

Nature immunology

Heikamp EB,Patel CH,Collins S,Waickman A,Oh MH,Sun IH,Illei P,Sharma A,Naray-Fejes-Toth A,Fejes-Toth G,Misra-Sen J,Horton MR,Powell JD

16-7123 was used in Neutralization experiments to characterise the range of phenotypes generated for mice with selective deletion of SGK1 in T cells.

Thu May 01 00:00:00 EDT 2014

The transcription factor Foxp1 is a critical negative regulator of the differentiation of follicular helper T cells.

Nature immunology

Wang H,Geng J,Wen X,Bi E,Kossenkov AV,Wolf AI,Tas J,Choi YS,Takata H,Day TJ,Chang LY,Sprout SL,Becker EK,Willen J,Tian L,Wang X,Xiao C,Jiang P,Crotty S,Victora GD,Showe LC,Tucker HO,Erikson J,Hu H

16-7123 was used in Functional assays to investigate the role of Foxp1 in CD4(+) cells, showing that it is a critical negative regulator of the differentiation of follicular helper T cells.

Tue Jul 01 00:00:00 EDT 2014

mTOR kinase inhibitors promote antibody class switching via mTORC2 inhibition.

Proceedings of the National Academy of Sciences of the United States of America

Limon JJ,So L,Jellbauer S,Chiu H,Corado J,Sykes SM,Raffatellu M,Fruman DA

16-7123 was used in in vitro experiments to further elucidate the complexity of mTOR signalling in B cells.

Tue Nov 25 00:00:00 EST 2014

IL-18, but not IL-12, induces production of IFN-γ in the immunosuppressive environment of HPV16 E7 transgenic hyperplastic skin.

The Journal of investigative dermatology

Gosmann C,Frazer IH,Mattarollo SR,Blumenthal A

16-7123 was used in Neutralization experiments to show that IL-18 contributes to inducing IFN-γ in an immunosuppressive cutaneous environment caused by viral oncogene-driven hyperplasia.

Wed Oct 01 00:00:00 EDT 2014

CX(3)CR1(+) macrophages support IL-22 production by innate lymphoid cells during infection with Citrobacter rodentium.

Mucosal immunology

Manta C,Heupel E,Radulovic K,Rossini V,Garbi N,Riedel CU,Niess JH

Published figure using IL-12/IL-23 p40 monoclonal antibody (Product # 16-7123-81) in Flow Cytometry

Tue Jan 01 00:00:00 EST 2013

Galectin-3 modulates Th17 responses by regulating dendritic cell cytokines.

The American journal of pathology

Fermin Lee A,Chen HY,Wan L,Wu SY,Yu JS,Huang AC,Miaw SC,Hsu DK,Wu-Hsieh BA,Liu FT

16-7123 was used in Neutralization experiments to demonstrate that galectin-3 suppresses Th17 responses by regulating dendritic cell cytokine production.

Tue Oct 01 00:00:00 EDT 2013

Selective activation of antigen-experienced T cells by anti-CD3 constrained on nanoparticles.

Journal of immunology (Baltimore, Md. : 1950)

Lo YC,Edidin MA,Powell JD

16-7123 was used in Neutralization experiments to show that constraining anti-CD3 on the surface of a nanoparticle selectively enhances proliferation and cytokine production of Ag-experienced T cells but does not activate naive T cells.

Fri Nov 15 00:00:00 EST 2013

Th17 cells induce Th1-polarizing monocyte-derived dendritic cells.

Journal of immunology (Baltimore, Md. : 1950)

Davidson MG,Alonso MN,Yuan R,Axtell RC,Kenkel JA,Suhoski MM,González JC,Steinman L,Engleman EG

16-7123 was used in Functional assays to investigate how murine Th cells influence the phenotype and function of monocytes.

Thu Aug 01 00:00:00 EDT 2013

Prostaglandin E₂ promotes Th1 differentiation via synergistic amplification of IL-12 signalling by cAMP and PI3-kinase.

Nature communications

Yao C,Hirata T,Soontrapa K,Ma X,Takemori H,Narumiya S

16-7123 was used in Functional assays to investigate the role of cAMP in Th1 differentiation, showing that prostaglandin E2 promotes differentiation via synergistic amplification of IL-12 signalling by cAMP and PI3-kinase.

Tue Oct 22 00:00:00 EDT 2013

Nicotinic receptor alpha7 expression identifies a novel hematopoietic progenitor lineage.

PloS one

Gahring LC,Enioutina EY,Myers EJ,Spangrude GJ,Efimova OV,Kelley TW,Tvrdik P,Capecchi MR,Rogers SW

16-7123 was used in Flow cytometry/Cell sorting to investigate how inflammatory responses are mechanistically modulated by nicotinic acetylcholine receptors.

Tue Aug 27 00:00:00 EDT 2013

Tumor-infiltrating DCs suppress nucleic acid-mediated innate immune responses through interactions between the receptor TIM-3 and the alarmin HMGB1.

Nature immunology

Chiba S,Baghdadi M,Akiba H,Yoshiyama H,Kinoshita I,Dosaka-Akita H,Fujioka Y,Ohba Y,Gorman JV,Colgan JD,Hirashima M,Uede T,Takaoka A,Yagita H,Jinushi M

16-7123 was used in Functional assays to define a mechanism whereby tumour microenvironments suppress antitumour immunity mediated by nucleic acids.

Sat Sep 01 00:00:00 EDT 2012

TLR4 and NKT cell synergy in immunotherapy against visceral leishmaniasis.

PLoS pathogens

Karmakar S,Bhaumik SK,Paul J,De T

16-7123 was used in Neutralization experiments to develop a defined, immunotherapeutic strategy for the treatment of immune diseases using a mouse model of visceral leishmaniasis.

Mon Aug 20 00:00:00 EDT 2012

Retinal self-antigen induces a predominantly Th1 effector response in Axl and Mertk double-knockout mice.

Journal of immunology (Baltimore, Md. : 1950)

Ye F,Han L,Lu Q,Dong W,Chen Z,Shao H,Kaplan HJ,Li Q,Lu Q

16-7123 was used in Neutralization experiments to study the molecular mechanism underlying the TAM regulation of APC functions and subsequent effects on the induction of an autoimmune response against the eye.

Sat Oct 15 00:00:00 EDT 2011

IL-18, but not IL-15, contributes to the IL-12-dependent induction of NK-cell effector functions by Leishmania infantum in vivo.

European journal of immunology

Haeberlein S,Sebald H,Bogdan C,Schleicher U

16-7123 was used in Neutralization experiments to investigate whether IL-15 or IL-18 mediate the activity of IL-12, showing that IL-18 contributes to the IL-12-dependent induction of NK-cell effector functions by Leishmania infantum.

Tue Jun 01 00:00:00 EDT 2010

Lck mediates Th2 differentiation through effects on T-bet and GATA-3.

Journal of immunology (Baltimore, Md. : 1950)

Kemp KL,Levin SD,Bryce PJ,Stein PL

16-7123 was used in Magnetic cell separation to imply that Lck mediates Th2 differentiation through effects on T-bet and GATA-3.

Thu Apr 15 00:00:00 EDT 2010

Development of murine lupus involves the combined genetic contribution of the SLAM and FcgammaR intervals within the Nba2 autoimmune susceptibility locus.

Journal of immunology (Baltimore, Md. : 1950)

Jørgensen TN,Alfaro J,Enriquez HL,Jiang C,Loo WM,Atencio S,Bupp MR,Mailloux CM,Metzger T,Flannery S,Rozzo SJ,Kotzin BL,Rosemblatt M,Bono MR,Erickson LD

16-7123 was used in an ELISpot assay to identify gene intervals within Nba2 that control the development of autoantibody-producing B cells.

Fri Jan 15 00:00:00 EST 2010

Kidney dendritic cell activation is required for progression of renal disease in a mouse model of glomerular injury.

The Journal of clinical investigation

Heymann F,Meyer-Schwesinger C,Hamilton-Williams EE,Hammerich L,Panzer U,Kaden S,Quaggin SE,Floege J,Gröne HJ,Kurts C

16-7123 was used in Flow cytometry/Cell sorting to investigate the role of several immune cell types within tubulointerstitial mononuclear cell infiltrates in the progression of kidney disease.

Fri May 01 00:00:00 EDT 2009

The Runx3 transcription factor augments Th1 and down-modulates Th2 phenotypes by interacting with and attenuating GATA3.

Journal of immunology (Baltimore, Md. : 1950)

Kohu K,Ohmori H,Wong WF,Onda D,Wakoh T,Kon S,Yamashita M,Nakayama T,Kubo M,Satake M

16-7123 was used in Functional assays to investigate the role of Runx3 in Th1 and Th2 cells.

Tue Dec 15 00:00:00 EST 2009

APRIL (TNFSF13) regulates collagen-induced arthritis, IL-17 production and Th2 response.

European journal of immunology

Xiao Y,Motomura S,Podack ER

16-7123 was used in an ELISA assay to study the effects of a proliferation-inducing ligand (APRIL) deficiency on the mouse immune system in vitro.

Mon Dec 01 00:00:00 EST 2008

Effects of CXCL10 on dendritic cell and CD4+ T-cell functions during Leishmania amazonensis infection.

Infection and immunity

Vasquez RE,Xin L,Soong L

16-7123 was used in Flow cytometry/Cell sorting to investigate the mechanism underlying enhanced immunity against L. amazonensis infection following CXC10 treatment, showing that treatment has an effect on dendritic cell and CD4+ T-cell functions.

Tue Jan 01 00:00:00 EST 2008

Bioinformatics

Protein Aliases: CLMF p35; CLMF p40; Cytotoxic lymphocyte maturation factor 35 kDa subunit; Cytotoxic lymphocyte maturation factor 40 kDa subunit; il 12; IL 12/IL 23p40; il 23; IL 23 p40; IL-12 subunit p35; IL-12 subunit p40; IL-12A; IL-12B; IL-23 subunit alpha; IL-23-A; IL-23p19; IL12 p40; IL23; il23; IL23 p41; ILN; Interleukin; Interleukin 12; Interleukin-12 subunit alpha; Interleukin-12 subunit beta; Interleukin-23 subunit alpha; Interleukin-23 subunit p19; Interleukin12; RP23-388G23.1

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Gene Aliases: Il-12a; Il-12b; IL-12p35; Il-12p40; IL-23; Il12a; Il12b; Il12p40; Il23a; Ll12a; p19; p35; p40

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UniProt ID: (Mouse) P43431, (Mouse) P43432, (Mouse) Q9EQ14

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Entrez Gene ID: (Mouse) 16159, (Mouse) 16160, (Mouse) 83430

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Function(s)

cytokine activity protein binding interleukin-23 receptor binding cytokine interleukin superfamily intercellular signal molecule transmembrane signal receptor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-12/IL-23 p40 Monoclonal Antibody (C17.8), Functional Grade, eBioscience™

IL-12/IL-23 p40 Antibody (16-7123-81) in Flow

Product Details

16-7123-81

Western Blot (WB)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Neutralization (Neu)

Functional Assay (Functional)

T-Cell Activation (TCA)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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