Invitrogen

CD54 (ICAM-1) Monoclonal Antibody (YN1/1.7.4), Biotin, eBioscience™

CD54 (ICAM-1) Antibody in Flow Cytometry (Flow)

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CD54 (ICAM-1) Antibody (13-0541-82) in Flow

Staining of C57BL/6 splenocytes with 0.06 µg of Rat IgG2b kappa Isotype Control Biotin (Product # 13-4031-82) (open histogram) or 0.06 µg of Anti-Mouse CD54 (ICAM-1) Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis.

CD54 (ICAM-1) Antibody in Immunocytochemistry (ICC/IF)

CD54 (ICAM-1) Antibody in Immunohistochemistry (IHC)

CD54 (ICAM-1) Antibody in Neutralization (Neu)

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Product Details

13-0541-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Flow Cytometry (Flow)

0.125 µg/test

View 8 publications 8 publications

Neutralization (Neu)

View 1 publication 1 publication

Functional Assay (Functional)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

YN1/1.7.4

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466480

Product Specific Information

Description: The YN1/1.7.4 monoclonal antibody reacts with mouse CD54 (InterCellular Adhesion Molecule-1, ICAM-1), a 90-110 kDa transmembrane glycoprotein expressed by monocytes, lymphocytes, dendritic cells, and endothelial cells. Expression of CD54 is upregulated on activated lymphocytes. Interaction of CD54 with its ligands CD11a and CD11b is important in the inflammatory response. The YN1/1.7.4 monoclonal antibody recognizes a different epitope than the eBioKAT-1 monclonal antibody.

Applications Reported: The YN1/1.7.4 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The YN1/1.7.4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Assay Identifies Nilotinib as an Inhibitor of Inflammation in Acute Myeloid Leukemia.

Journal of medicinal chemistry

Marín-Rubio JL,Peltier-Heap RE,Dueñas ME,Heunis T,Dannoura A,Inns J,Scott J,Simpson AJ,Blair HJ,Heidenreich O,Allan JM,Watt JE,Martin MP,Saxty B,Trost M

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 13-0541-82) in Flow Cytometry

Thu Sep 22 00:00:00 EDT 2022

ICAM-1 and ICAM-2 Are Differentially Expressed and Up-Regulated on Inflamed Pulmonary Epithelium, but Neither ICAM-2 nor LFA-1: ICAM-1 Are Required for Neutrophil Migration Into the Airways In Vivo.

Frontiers in immunology

Chong DLW,Rebeyrol C,José RJ,Williams AE,Brown JS,Scotton CJ,Porter JC

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 13-0541-82) in Immunohistochemistry

Mon Oct 25 00:00:00 EDT 2021

Hemodynamic regulation of perivalvular endothelial gene expression prevents deep venous thrombosis.

The Journal of clinical investigation

Welsh JD,Hoofnagle MH,Bamezai S,Oxendine M,Lim L,Hall JD,Yang J,Schultz S,Engel JD,Kume T,Oliver G,Jimenez JM,Kahn ML

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 13-0541-82) in Immunohistochemistry

Mon Dec 02 00:00:00 EST 2019

Conserved regulation of RNA processing in somatic cell reprogramming.

BMC genomics

Kanitz A,Syed AP,Kaji K,Zavolan M

13-0541 was used in Flow cytometry/Cell sorting to provide a general resource for gene expression and splicing changes that take place during somatic cell reprogramming.

Thu Jan 31 00:00:00 EST 2019

PAI1 mediates fibroblast-mast cell interactions in skin fibrosis.

The Journal of clinical investigation

Pincha N,Hajam EY,Badarinath K,Batta SPR,Masudi T,Dey R,Andreasen P,Kawakami T,Samuel R,George R,Danda D,Jacob PM,Jamora C

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 13-0541-82) in Neutralization

Tue May 01 00:00:00 EDT 2018

Low-Dose Radiotherapy Has No Harmful Effects on Key Cells of Healthy Non-Inflamed Joints.

International journal of molecular sciences

Deloch L,Rückert M,Fietkau R,Frey B,Gaipl US

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 13-0541-82) in Flow Cytometry

Tue Oct 16 00:00:00 EDT 2018

Constitutively Active SMAD2/3 Are Broad-Scope Potentiators of Transcription-Factor-Mediated Cellular Reprogramming.

Cell stem cell

Ruetz T,Pfisterer U,Di Stefano B,Ashmore J,Beniazza M,Tian TV,Kaemena DF,Tosti L,Tan W,Manning JR,Chantzoura E,Ottosson DR,Collombet S,Johnsson A,Cohen E,Yusa K,Linnarsson S,Graf T,Parmar M,Kaji K

Thu Dec 07 00:00:00 EST 2017

Palmitoyl acyltransferase DHHC21 mediates endothelial dysfunction in systemic inflammatory response syndrome.

Nature communications

Beard RS,Yang X,Meegan JE,Overstreet JW,Yang CG,Elliott JA,Reynolds JJ,Cha BJ,Pivetti CD,Mitchell DA,Wu MH,Deschenes RJ,Yuan SY

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 13-0541-82) in Immunofluorescence

Thu Sep 22 00:00:00 EDT 2016

Reprogramming Roadblocks Are System Dependent.

Stem cell reports

Chantzoura E,Skylaki S,Menendez S,Kim SI,Johnsson A,Linnarsson S,Woltjen K,Chambers I,Kaji K

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 13-0541-82) in Flow Cytometry

Tue Sep 08 00:00:00 EDT 2015

Dietary gluten increases natural killer cell cytotoxicity and cytokine secretion.

European journal of immunology

Larsen J,Dall M,Antvorskov JC,Weile C,Engkilde K,Josefsen K,Buschard K

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 13-0541-82) in Flow Cytometry

Wed Oct 01 00:00:00 EDT 2014

High-resolution analysis with novel cell-surface markers identifies routes to iPS cells.

Nature

O'Malley J,Skylaki S,Iwabuchi KA,Chantzoura E,Ruetz T,Johnsson A,Tomlinson SR,Linnarsson S,Kaji K

13-0541 was used in Flow cytometry/Cell sorting to investigate the routes that fibroblasts take during the generation of induced pluripotent stem cells, showing that there are orderly changes in cell-surface markers.

Thu Jul 04 00:00:00 EDT 2013

A role for LFA-1 in delaying T-lymphocyte egress from lymph nodes.

The EMBO journal

Reichardt P,Patzak I,Jones K,Etemire E,Gunzer M,Hogg N

13-0541 was used in Immunofluorescence to study the role of the leukocyte function-associated antigen-1 (LFA-1) during T cell migration within and away from the lymph nodes.

Wed Mar 20 00:00:00 EDT 2013

In vivo measurement of the mouse pulmonary endothelial surface layer.

Journal of visualized experiments : JoVE

Yang Y,Yang G,Schmidt EP

13-0541 was used in Immunofluorescence to demonstrate how closed-chest intravital microscopy of a freely-moving mouse lung can be used to measure glycocalyx integrity via ESL exclusion of fluorescently-labeled high molecular weight dextrans from the endothelial surface.

Fri Feb 22 00:00:00 EST 2013

Defining the transcriptional and cellular landscape of type 1 diabetes in the NOD mouse.

PloS one

Carrero JA,Calderon B,Towfic F,Artyomov MN,Unanue ER

13-0541 was used in Immunofluorescence to examine autoimmune diabetes and its disease progression markers.

Mon Sep 30 00:00:00 EDT 2013

The pulmonary endothelial glycocalyx regulates neutrophil adhesion and lung injury during experimental sepsis.

Nature medicine

Schmidt EP,Yang Y,Janssen WJ,Gandjeva A,Perez MJ,Barthel L,Zemans RL,Bowman JC,Koyanagi DE,Yunt ZX,Smith LP,Cheng SS,Overdier KH,Thompson KR,Geraci MW,Douglas IS,Pearse DB,Tuder RM

13-0541 was used in Functional assays to elucidate the mechanisms by which glycocalyx loss occurs during sepsis, and how this allows for neutrophil adhesion within the pulmonary circulation.

Wed Aug 01 00:00:00 EDT 2012

Direct stimulation of T cells by membrane vesicles from antigen-presenting cells.

Proceedings of the National Academy of Sciences of the United States of America

Kovar M,Boyman O,Shen X,Hwang I,Kohler R,Sprent J

13-0541 was used in Flow cytometry/Cell sorting to study how dissociated cell membrane fragments from a DC line can be used as an effective substitute for viable DC.

Tue Aug 01 00:00:00 EDT 2006

Bioinformatics

Protein Aliases: CD54; Human rhinovirus receptor; ICAM-1; Intercellular adhesion molecule 1; Ly 47; MALA-2; MALA2; MyD10; sCD54; soluble CD54; Surface antigen of activated B cells

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Gene Aliases: CD54; Icam-1; Icam1; Ly-47; MALA-2

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UniProt ID: (Mouse) P13597

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Entrez Gene ID: (Mouse) 15894

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Function(s)

integrin binding protein complex binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD54 (ICAM-1) Monoclonal Antibody (YN1/1.7.4), Biotin, eBioscience™

CD54 (ICAM-1) Antibody (13-0541-82) in Flow

Product Details

13-0541-82

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Neutralization (Neu)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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