Invitrogen

CD54 (ICAM-1) Monoclonal Antibody (YN1/1.7.4), eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

11 Published Figures

26 References

View all (173) ICAM-1 (CD54) antibodies

Datasheet

Protocols

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Datasheet

Protocols

Questions & Answers

CD54 (ICAM-1) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 13

CD54 (ICAM-1) Antibody (14-0541-82) in Flow

Staining of mouse splenocytes with Anti-Mouse CD54 (ICAM-1) FITC (left) and PE (right). Autofluorescence is shown via open histogram. Total viable cells were used for analysis.

CD54 (ICAM-1) Antibody in Immunocytochemistry (ICC/IF)

CD54 (ICAM-1) Antibody in Neutralization (Neu)

Product Image Gallery

Product Details

14-0541-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

Assay-Dependent

View 5 publications 5 publications

Immunocytochemistry (ICC/IF)

View 5 publications 5 publications

Flow Cytometry (Flow)

0.5 µg/test

View 11 publications 11 publications

Neutralization (Neu)

Assay-Dependent

View 4 publications 4 publications

Functional Assay (Functional)

Assay-Dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

YN1/1.7.4

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_467301

Product Specific Information

Description: The YN1/1.7.4 monoclonal antibody reacts with mouse CD54 (InterCellular Adhesion Molecule-1, ICAM-1), a 90-110 kDa transmembrane glycoprotein expressed by monocytes, lymphocytes, dendritic cells, and endothelial cells. Expression of CD54 is upregulated on activated lymphocytes. Interaction of CD54 with its ligands CD11a and CD11b is important in the inflammatory response. The YN1/1.7.4 monoclonal antibody recognizes a different epitope than the eBioKAT-1 monclonal antibody.

Applications Reported: The YN1/1.7.4 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining. YN1/1.7.4 has also been reported in blocking of CD54 in functional studies. (Please use Functional Grade purified YN1/1.7.4, Product # 16-0541, in functional assays).

Applications Tested: The YN1/1.7.4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Assay Identifies Nilotinib as an Inhibitor of Inflammation in Acute Myeloid Leukemia.

Journal of medicinal chemistry

Marín-Rubio JL,Peltier-Heap RE,Dueñas ME,Heunis T,Dannoura A,Inns J,Scott J,Simpson AJ,Blair HJ,Heidenreich O,Allan JM,Watt JE,Martin MP,Saxty B,Trost M

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 14-0541-82) in Flow Cytometry

Thu Sep 22 00:00:00 EDT 2022

ICAM-1 and ICAM-2 Are Differentially Expressed and Up-Regulated on Inflamed Pulmonary Epithelium, but Neither ICAM-2 nor LFA-1: ICAM-1 Are Required for Neutrophil Migration Into the Airways In Vivo.

Frontiers in immunology

Chong DLW,Rebeyrol C,José RJ,Williams AE,Brown JS,Scotton CJ,Porter JC

14-0541-82 was used in Immunohistochemistry to suggest that although β2 integrins interacting with ICAMs may regulate low levels of neutrophil traffic in healthy lung or early in inflammation when the epithelial barrier is intact; these interactions may be redundant later in inflammation when epithelial junctions are disrupted and no longer limit TEpM.

Mon Oct 25 00:00:00 EDT 2021

Frontiers in immunology

Chong DLW,Rebeyrol C,José RJ,Williams AE,Brown JS,Scotton CJ,Porter JC

Mon Oct 25 00:00:00 EDT 2021

Neutrophil-endothelial interactions of murine cells is not a good predictor of their interactions in human cells.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Soroush F,Tang Y,Mustafa O,Sun S,Yang Q,Kilpatrick LE,Kiani MF

14-0541-81 was used in Immunocytochemistry to determine whether the response of murine cells to inflammatory activation or anti-inflammatory treatment is predictive of the response in human cells.

Sat Feb 01 00:00:00 EST 2020

L-Selectin/CD62L is a Key Driver of Non-Alcoholic Steatohepatitis in Mice and Men.

Cells

Drescher HK,Schippers A,Rosenhain S,Gremse F,Bongiovanni L,Bruin A,Eswaran S,Gallage SU,Pfister D,Szydlowska M,Heikenwalder M,Weiskirchen S,Wagner N,Trautwein C,Weiskirchen R,Kroy DC

14-0541 was used in Immunohistochemistry-immunofluorescence to investigate the functional role of CD62L in NASH in humans as well as in two mouse models of steatohepatitis.

Wed Apr 29 00:00:00 EDT 2020

Conserved regulation of RNA processing in somatic cell reprogramming.

BMC genomics

Kanitz A,Syed AP,Kaji K,Zavolan M

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 14-0541-82) in Flow Cytometry

Thu Jan 31 00:00:00 EST 2019

PAI1 mediates fibroblast-mast cell interactions in skin fibrosis.

The Journal of clinical investigation

Pincha N,Hajam EY,Badarinath K,Batta SPR,Masudi T,Dey R,Andreasen P,Kawakami T,Samuel R,George R,Danda D,Jacob PM,Jamora C

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 14-0541-82) in Neutralization

Tue May 01 00:00:00 EDT 2018

PAI1 mediates fibroblast-mast cell interactions in skin fibrosis.

The Journal of clinical investigation

Pincha N,Hajam EY,Badarinath K,Batta SPR,Masudi T,Dey R,Andreasen P,Kawakami T,Samuel R,George R,Danda D,Jacob PM,Jamora C

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 14-0541-82) in Neutralization

Tue May 01 00:00:00 EDT 2018

Low-Dose Radiotherapy Has No Harmful Effects on Key Cells of Healthy Non-Inflamed Joints.

International journal of molecular sciences

Deloch L,Rückert M,Fietkau R,Frey B,Gaipl US

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 14-0541-82) in Flow Cytometry

Tue Oct 16 00:00:00 EDT 2018

Constitutively Active SMAD2/3 Are Broad-Scope Potentiators of Transcription-Factor-Mediated Cellular Reprogramming.

Cell stem cell

Ruetz T,Pfisterer U,Di Stefano B,Ashmore J,Beniazza M,Tian TV,Kaemena DF,Tosti L,Tan W,Manning JR,Chantzoura E,Ottosson DR,Collombet S,Johnsson A,Cohen E,Yusa K,Linnarsson S,Graf T,Parmar M,Kaji K

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 14-0541-82) in Flow Cytometry

Thu Dec 07 00:00:00 EST 2017

Endogenous TNFα orchestrates the trafficking of neutrophils into and within lymphatic vessels during acute inflammation.

Scientific reports

Arokiasamy S,Zakian C,Dilliway J,Wang W,Nourshargh S,Voisin MB

14-0541 was used in Immunofluorescence to demonstrate a new role for TNFα as a key regulator of neutrophil trafficking into and within the lymphatic system.

Mon Mar 13 00:00:00 EDT 2017

A robust in vitro model for trans-lymphatic endothelial migration.

Scientific reports

Xiong Y,Brinkman CC,Famulski KS,Mongodin EF,Lord CJ,Hippen KL,Blazar BR,Bromberg JS

14-0541 was used in Flow cytometry/Cell sorting to establish an in vitro system to evaluate lymphatic Trans-endothelial migration for various cell types across primary mouse and human lymphatic endothelial cells.

Tue May 09 00:00:00 EDT 2017

Palmitoyl acyltransferase DHHC21 mediates endothelial dysfunction in systemic inflammatory response syndrome.

Nature communications

Beard RS,Yang X,Meegan JE,Overstreet JW,Yang CG,Elliott JA,Reynolds JJ,Cha BJ,Pivetti CD,Mitchell DA,Wu MH,Deschenes RJ,Yuan SY

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 14-0541-82) in Immunofluorescence

Thu Sep 22 00:00:00 EDT 2016

Inhibition of the TNF Family Cytokine RANKL Prevents Autoimmune Inflammation in the Central Nervous System.

Immunity

Guerrini MM,Okamoto K,Komatsu N,Sawa S,Danks L,Penninger JM,Nakashima T,Takayanagi H

14-0541-82 was used in Flow Cytometry to show that receptor activator of nuclear factor-κB (NF-κB) ligand (RANKL) on T cells regulates C-C type chemokine ligand 20 (CCL20) production by astrocytes and T cell localization in the CNS.

Tue Dec 15 00:00:00 EST 2015

Reprogramming Roadblocks Are System Dependent.

Stem cell reports

Chantzoura E,Skylaki S,Menendez S,Kim SI,Johnsson A,Linnarsson S,Woltjen K,Chambers I,Kaji K

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 14-0541-82) in Flow Cytometry

Tue Sep 08 00:00:00 EDT 2015

Neutrophil crawling in capillaries; a novel immune response to Staphylococcus aureus.

PLoS pathogens

Harding MG,Zhang K,Conly J,Kubes P

14-0541 was used in Blocking experiments to elucidate the importance of neutrophils in protection against MRSA-induced soft tissue infections.

Wed Oct 01 00:00:00 EDT 2014

Dietary gluten increases natural killer cell cytotoxicity and cytokine secretion.

European journal of immunology

Larsen J,Dall M,Antvorskov JC,Weile C,Engkilde K,Josefsen K,Buschard K

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 14-0541-82) in Flow Cytometry

Wed Oct 01 00:00:00 EDT 2014

High-resolution analysis with novel cell-surface markers identifies routes to iPS cells.

Nature

O'Malley J,Skylaki S,Iwabuchi KA,Chantzoura E,Ruetz T,Johnsson A,Tomlinson SR,Linnarsson S,Kaji K

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 14-0541-82) in Flow Cytometry

Thu Jul 04 00:00:00 EDT 2013

ICAM-1-mediated leukocyte adhesion is critical for the activation of endothelial LSP1.

American journal of physiology. Cell physiology

Hossain M,Qadri SM,Su Y,Liu L

14-0541 was used in Functional assays to study the role of endothelial leukocyte-specific protein 1 in leukocyte adhesion through ICAM-1.

Wed May 01 00:00:00 EDT 2013

Pericytes support neutrophil subendothelial cell crawling and breaching of venular walls in vivo.

The Journal of experimental medicine

Proebstl D,Voisin MB,Woodfin A,Whiteford J,D'Acquisto F,Jones GE,Rowe D,Nourshargh S

14-0541 was used in Immunofluorescence to identify previously unknown roles for pericytes in neutrophil transmigration in vivo and leukocyte trafficking into sites of inflammation.

Mon Jun 04 00:00:00 EDT 2012

Cutting edge: Humanized nano-proresolving medicines mimic inflammation-resolution and enhance wound healing.

Journal of immunology (Baltimore, Md. : 1950)

Norling LV,Spite M,Yang R,Flower RJ,Perretti M,Serhan CN

14-0541 was used in Flow cytometry/Cell sorting to study the temporal generation and properties of endogenous microparticles produced in evolving self-limited inflammatory exudates in vivo.

Sun May 15 00:00:00 EDT 2011

Requirement of interaction between mast cells and skin dendritic cells to establish contact hypersensitivity.

PloS one

Otsuka A,Kubo M,Honda T,Egawa G,Nakajima S,Tanizaki H,Kim B,Matsuoka S,Watanabe T,Nakae S,Miyachi Y,Kabashima K

14-0541 was used in Neutralization experiments to investigate the role of mast cells in contact hypersensitivity, showing that interaction between mast cells and skin dendritic cells is required.

Thu Jan 26 00:00:00 EST 2012

Steroid-resistant lymphatic remodeling in chronically inflamed mouse airways.

The American journal of pathology

Yao LC,Baluk P,Feng J,McDonald DM

14-0541 was used in Immunohistochemistry to suggest that lymphatic remodelling after the initial inflammatory response may influence later inflammatory episodes.

Mon Mar 01 00:00:00 EST 2010

Annexin-1-deficient dendritic cells acquire a mature phenotype during differentiation.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Huggins A,Paschalidis N,Flower RJ,Perretti M,D'Acquisto F

14-0541 was used in Flow cytometry/Cell sorting to suggest that the inhibition of Anx-A1 expression in dendritic cells may be useful to modulate the adaptive immune response in pathogen-induced immune diseases.

Wed Apr 01 00:00:00 EDT 2009

Microvascular endothelial cells exhibit optimal aspect ratio for minimizing flow resistance.

Annals of biomedical engineering

Sumagin R,Brown CW,Sarelius IH,King MR

14-0541 was used in Immunofluorescence to reveal that the spacing of endethelial nuclei in venules is similar to a predicted optimal configuration to minimise flow resistance.

Tue Apr 01 00:00:00 EDT 2008

Murine cerebral malaria development is independent of toll-like receptor signaling.

The American journal of pathology

Togbe D,Schofield L,Grau GE,Schnyder B,Boissay V,Charron S,Rose S,Beutler B,Quesniaux VF,Ryffel B

14-0541 was used in Immunohistochemistry to investigate innate immune activation by malarial molecular motifs and the potential underlying signalling mechanisms.

Tue May 01 00:00:00 EDT 2007

Prevention of lipopolysaccharide-induced microangiopathy by gp49B1: evidence for an important role for gp49B1 expression on neutrophils.

The Journal of experimental medicine

Zhou JS,Friend DS,Feldweg AM,Daheshia M,Li L,Austen KF,Katz HR

14-0541 was used in Blocking experiments to investigate the role of gp49B1 in neutrophils, showing that gp49B1 expression on neutrophils suppresses LPS-induced microangiopathy.

Mon Oct 20 00:00:00 EDT 2003

Bioinformatics

Protein Aliases: CD54; Human rhinovirus receptor; ICAM-1; Intercellular adhesion molecule 1; Ly 47; MALA-2; MALA2; MyD10; sCD54; soluble CD54; Surface antigen of activated B cells

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Gene Aliases: CD54; Icam-1; Icam1; Ly-47; MALA-2

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UniProt ID: (Mouse) P13597

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Entrez Gene ID: (Mouse) 15894

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Function(s)

integrin binding protein complex binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD54 (ICAM-1) Monoclonal Antibody (YN1/1.7.4), eBioscience™

CD54 (ICAM-1) Antibody (14-0541-82) in Flow

Product Details

14-0541-82

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Neutralization (Neu)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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