Invitrogen

CD54 (ICAM-1) Monoclonal Antibody (YN1/1.7.4), PE, eBioscience™

CD54 (ICAM-1) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 10

CD54 (ICAM-1) Antibody (12-0541-81) in Flow

Staining of C57BL/6 splenocytes with 0.06 µg of Rat IgG2b kappa Isotype Control PE (Product # 12-4031-82) (open histogram) or 0.06 µg of Anti-Mouse CD54 (ICAM-1) PE (filled histogram). Total viable cells were used for analysis.

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Product Details

12-0541-81

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

0.125 µg/test

View 26 publications 26 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

YN1/1.7.4

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465706

Product Specific Information

Description: The YN1/1.7.4 monoclonal antibody reacts with mouse CD54 (InterCellular Adhesion Molecule-1, ICAM-1), a 90-110 kDa transmembrane glycoprotein expressed by monocytes, lymphocytes, dendritic cells, and endothelial cells. Expression of CD54 is upregulated on activated lymphocytes. Interaction of CD54 with its ligands CD11a and CD11b is important in the inflammatory response. The YN1/1.7.4 monoclonal antibody recognizes a different epitope than the eBioKAT-1 monclonal antibody.

Applications Reported: The YN1/1.7.4 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The YN1/1.7.4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

IL6Myc mouse is an immunocompetent model for the development of aggressive multiple myeloma.

Haematologica

Pisano MD,Sun F,Cheng Y,Parashar D,Zhou V,Jing X,Sompallae R,Abrudan J,Zimmermann MT,Mathison A,Janz S,Pufall MA

12-0541-81 was used in Flow cytometry/Cell sorting to indicate that the IL6Myc model is useful for studying development of aggressive MM and for developing new treatments against such forms of the disease.

Fri Dec 01 00:00:00 EST 2023

A Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Assay Identifies Nilotinib as an Inhibitor of Inflammation in Acute Myeloid Leukemia.

Journal of medicinal chemistry

Marín-Rubio JL,Peltier-Heap RE,Dueñas ME,Heunis T,Dannoura A,Inns J,Scott J,Simpson AJ,Blair HJ,Heidenreich O,Allan JM,Watt JE,Martin MP,Saxty B,Trost M

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 12-0541-81) in Flow Cytometry

Thu Sep 22 00:00:00 EDT 2022

Innate Immune Dysfunction in Rosacea Promotes Photosensitivity and Vascular Adhesion Molecule Expression.

The Journal of investigative dermatology

Kulkarni NN,Takahashi T,Sanford JA,Tong Y,Gombart AF,Hinds B,Cheng JY,Gallo RL

12-0541 was used in Immunohistochemistry to show how the expression of LL-37 can lead to enhanced sensitivity to UVB radiation in rosacea.

Sun Mar 01 00:00:00 EST 2020

Regulatory T cells mediate specific suppression by depleting peptide-MHC class II from dendritic cells.

Nature immunology

Akkaya B,Oya Y,Akkaya M,Al Souz J,Holstein AH,Kamenyeva O,Kabat J,Matsumura R,Dorward DW,Glass DD,Shevach EM

12-0541-81 was used in Flow Cytometry to study whether pathways similar to the multiple suppressive mechansims triggered in vitro by T cell antigen receptor activated regulatory T cells exist in vivo.

Fri Feb 01 00:00:00 EST 2019

Conserved regulation of RNA processing in somatic cell reprogramming.

BMC genomics

Kanitz A,Syed AP,Kaji K,Zavolan M

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 12-0541-81) in Flow Cytometry

Thu Jan 31 00:00:00 EST 2019

Bovine κ-Casein Fragment Induces Hypo-Responsive M2-Like Macrophage Phenotype.

Nutrients

Lalor R,O'Neill S

12-0541 was used in Flow cytometry/Cell sorting to investigate the possible therapeutic effects bovine casein derived nutraceuticals exert on macrophage immunological function.

Tue Jul 23 00:00:00 EDT 2019

Persistence of Integrase-Deficient Lentiviral Vectors Correlates with the Induction of STING-Independent CD8⁺ T Cell Responses.

Cell reports

Cousin C,Oberkampf M,Felix T,Rosenbaum P,Weil R,Fabrega S,Morante V,Negri D,Cara A,Dadaglio G,Leclerc C

12-0541 was used in Flow cytometry/Cell sorting to demonstrate that integrase-deficient lentiviral vectors can trigger persistent adaptive response by the mediation of a weak and transient innate response alongside the persistence of the vector in tissues.

Tue Jan 29 00:00:00 EST 2019

Low-Dose Radiotherapy Has No Harmful Effects on Key Cells of Healthy Non-Inflamed Joints.

International journal of molecular sciences

Deloch L,Rückert M,Fietkau R,Frey B,Gaipl US

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 12-0541-81) in Flow Cytometry

Tue Oct 16 00:00:00 EDT 2018

Low-Dose Radiotherapy Ameliorates Advanced Arthritis in hTNF-α tg Mice by Particularly Positively Impacting on Bone Metabolism.

Frontiers in immunology

Deloch L,Derer A,Hueber AJ,Herrmann M,Schett GA,Wölfelschneider J,Hahn J,Rühle PF,Stillkrieg W,Fuchs J,Fietkau R,Frey B,Gaipl US

12-0541 was used in Flow cytometry/Cell sorting to study the underlying mechanisms by which low-dose radiotherapy mediates anti-inflammatory and pain-reducing effects in non-linear dose response relationships.

Mon Sep 30 00:00:00 EDT 2019

Constitutively Active SMAD2/3 Are Broad-Scope Potentiators of Transcription-Factor-Mediated Cellular Reprogramming.

Cell stem cell

Ruetz T,Pfisterer U,Di Stefano B,Ashmore J,Beniazza M,Tian TV,Kaemena DF,Tosti L,Tan W,Manning JR,Chantzoura E,Ottosson DR,Collombet S,Johnsson A,Cohen E,Yusa K,Linnarsson S,Graf T,Parmar M,Kaji K

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 12-0541-81) in Flow Cytometry

Thu Dec 07 00:00:00 EST 2017

Galectin-3: A Positive Regulator of Leukocyte Recruitment in the Inflamed Microcirculation.

Journal of immunology (Baltimore, Md. : 1950)

Gittens BR,Bodkin JV,Nourshargh S,Perretti M,Cooper D

12-0541 was used in Flow cytometry/Cell sorting to show that mechanisms mediating leukocyte slow rolling and emigration are impaired in Gal-3-/- mice, which could be because of impaired expression of cell adhesion molecules and an altered cell surface glycoproteome.

Thu Jun 01 00:00:00 EDT 2017

Neutrophil ageing is regulated by the microbiome.

Nature

Zhang D,Chen G,Manwani D,Mortha A,Xu C,Faith JJ,Burk RD,Kunisaki Y,Jang JE,Scheiermann C,Merad M,Frenette PS

12-0541 was used in Flow cytometry/Cell sorting to show that neutrophil pro-inflammatory activity correlates positively with their ageing whilst in circulation.

Thu Sep 24 00:00:00 EDT 2015

Neutrophil ageing is regulated by the microbiome.

Nature

Zhang D,Chen G,Manwani D,Mortha A,Xu C,Faith JJ,Burk RD,Kunisaki Y,Jang JE,Scheiermann C,Merad M,Frenette PS

12-0541 was used in Flow cytometry/Cell sorting to show that neutrophil pro-inflammatory activity correlates positively with their ageing whilst in circulation.

Thu Sep 24 00:00:00 EDT 2015

The olive oil-based lipid clinoleic blocks leukocyte recruitment and improves survival during systemic inflammation: a comparative in vivo study of different parenteral lipid emulsions.

Mediators of inflammation

Buschmann K,Poeschl J,Braach N,Hudalla H,Kuss N,Frommhold D

12-0541 was used in Flow cytometry/Cell sorting to demonstrate that the anti-inflammatory properties of Clinoleic are superior to those of Smoflipid and Lipofundin even during systemic inflammation.

Mon Jan 11 00:00:00 EST 2016

Reprogramming Roadblocks Are System Dependent.

Stem cell reports

Chantzoura E,Skylaki S,Menendez S,Kim SI,Johnsson A,Linnarsson S,Woltjen K,Chambers I,Kaji K

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 12-0541-81) in Flow Cytometry

Tue Sep 08 00:00:00 EDT 2015

CLEC-2 is required for development and maintenance of lymph nodes.

Blood

Bénézech C,Nayar S,Finney BA,Withers DR,Lowe K,Desanti GE,Marriott CL,Watson SP,Caamaño JH,Buckley CD,Barone F

12-0541 was used in Flow cytometry/Cell sorting to investigate the role that CLEC-2 plays in the development and maintenance of lymph nodes.

Thu May 15 00:00:00 EDT 2014

Dietary gluten increases natural killer cell cytotoxicity and cytokine secretion.

European journal of immunology

Larsen J,Dall M,Antvorskov JC,Weile C,Engkilde K,Josefsen K,Buschard K

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 12-0541-81) in Flow Cytometry

Wed Oct 01 00:00:00 EDT 2014

RAGE controls activation and anti-inflammatory signalling of protein C.

PloS one

Braach N,Frommhold D,Buschmann K,Pflaum J,Koch L,Hudalla H,Staudacher K,Wang H,Isermann B,Nawroth P,Poeschl J

12-0541 was used in Flow cytometry/Cell sorting to provide the first evidence of interplay between the receptor for advanced glycation endproducts (RAGE) and the protein C pathway in inflammation.

Mon Dec 22 00:00:00 EST 2014

High-resolution analysis with novel cell-surface markers identifies routes to iPS cells.

Nature

O'Malley J,Skylaki S,Iwabuchi KA,Chantzoura E,Ruetz T,Johnsson A,Tomlinson SR,Linnarsson S,Kaji K

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 12-0541-81) in Flow Cytometry

Thu Jul 04 00:00:00 EDT 2013

The sensing of environmental stimuli by follicular dendritic cells promotes immunoglobulin A generation in the gut.

Immunity

Suzuki K,Maruya M,Kawamoto S,Sitnik K,Kitamura H,Agace WW,Fagarasan S

12-0541 was used in Flow cytometry/Cell sorting to investigate whether follicular dendritic cells within the germinal centers directly contribute to the IgA production in Peyer's patches, showing that sensing of environmental stimuli promotes IgA production.

Fri Jul 23 00:00:00 EDT 2010

Stress-regulated targeting of the NKG2D ligand Mult1 by a membrane-associated RING-CH family E3 ligase.

Journal of immunology (Baltimore, Md. : 1950)

Nice TJ,Deng W,Coscoy L,Raulet DH

12-0541 was used in Flow cytometry/Cell sorting to investigate the regulation of cell surface expression of the NKG2D ligand, Mult1.

Mon Nov 01 00:00:00 EDT 2010

Ontogeny of stromal organizer cells during lymph node development.

Journal of immunology (Baltimore, Md. : 1950)

Bénézech C,White A,Mader E,Serre K,Parnell S,Pfeffer K,Ware CF,Anderson G,Caamaño JH

12-0541 was used in Immunofluorescence to highlight the importance of the signals and cellular interactions that induce the maturation of stromal cells.

Thu Apr 15 00:00:00 EDT 2010

Ontogeny of stromal organizer cells during lymph node development.

Journal of immunology (Baltimore, Md. : 1950)

Bénézech C,White A,Mader E,Serre K,Parnell S,Pfeffer K,Ware CF,Anderson G,Caamaño JH

12-0541 was used in Immunofluorescence to highlight the importance of the signals and cellular interactions that induce the maturation of stromal cells.

Thu Apr 15 00:00:00 EDT 2010

Absence of IFN-beta impairs antigen presentation capacity of splenic dendritic cells via down-regulation of heat shock protein 70.

Journal of immunology (Baltimore, Md. : 1950)

Zietara N,Łyszkiewicz M,Gekara N,Puchałka J,Dos Santos VA,Hunt CR,Pandita TK,Lienenklaus S,Weiss S

12-0541 was used in Flow cytometry/Cell sorting to investigate the role of low level production of IFN, showing that its absence impairs antigen presentation capacity of splenic dendritic cells.

Wed Jul 15 00:00:00 EDT 2009

A role for cFLIP in B cell proliferation and stress MAPK regulation.

Journal of immunology (Baltimore, Md. : 1950)

Zhang H,Rosenberg S,Coffey FJ,He YW,Manser T,Hardy RR,Zhang J

12-0541 was used in Flow cytometry/Cell sorting to reveal the novel functions of cFLIP in B cells.

Thu Jan 01 00:00:00 EST 2009

Interleukin-10 overexpression promotes Fas-ligand-dependent chronic macrophage-mediated demyelinating polyneuropathy.

PloS one

Dace DS,Khan AA,Stark JL,Kelly J,Cross AH,Apte RS

12-0541 was used in Immunohistochemistry on paraffin embedded tissues to investigate the conditions contributing to the development of CIDP and related conditions.

Tue Sep 22 00:00:00 EDT 2009

Endothelial CD47 interaction with SIRPgamma is required for human T-cell transendothelial migration under shear flow conditions in vitro.

Blood

Stefanidakis M,Newton G,Lee WY,Parkos CA,Luscinskas FW

12-0541 was used in Flow cytometry/Cell sorting to test whether CD47-SIRPgamma interactions affect T-cell transmigration using blocking mAb against CD47 or SIRPgamma.

Fri Aug 15 00:00:00 EDT 2008

Lymphotoxin a-dependent and -independent signals regulate stromal organizer cell homeostasis during lymph node organogenesis.

Blood

White A,Carragher D,Parnell S,Msaki A,Perkins N,Lane P,Jenkinson E,Anderson G,Caamaño JH

12-0541 was used in Flow cytometry/Cell sorting to further investigate the mechanisms that regulate the development of stromal organiser cells.

Sat Sep 15 00:00:00 EDT 2007

The Fas-associated death domain protein is required in apoptosis and TLR-induced proliferative responses in B cells.

Journal of immunology (Baltimore, Md. : 1950)

Imtiyaz HZ,Rosenberg S,Zhang Y,Rahman ZS,Hou YJ,Manser T,Zhang J

12-0541 was used in Flow cytometry/Cell sorting to show that the deletion of the Fas-associated death domain protein at the pro-B cell stage has effects on B cell apoptosis and development.

Thu Jun 01 00:00:00 EDT 2006

T/B lineage choice occurs prior to intrathymic Notch signaling.

Blood

Harman BC,Jenkinson WE,Parnell SM,Rossi SW,Jenkinson EJ,Anderson G

12-0541 was used in Flow cytometry/Cell sorting to analyse the T-cell/B-cell lineage potential of precursors that have been recruited to the foetal mouse thymus, showing that lineage choice occurs prior to intrathymic Notch signaling.

Mon Aug 01 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: CD54; Human rhinovirus receptor; ICAM-1; Intercellular adhesion molecule 1; Ly 47; MALA-2; MALA2; MyD10; sCD54; soluble CD54; Surface antigen of activated B cells

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Gene Aliases: CD54; Icam-1; Icam1; Ly-47; MALA-2

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UniProt ID: (Mouse) P13597

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Entrez Gene ID: (Mouse) 15894

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Function(s)

integrin binding protein complex binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD54 (ICAM-1) Monoclonal Antibody (YN1/1.7.4), PE, eBioscience™

CD54 (ICAM-1) Antibody (12-0541-81) in Flow

Product Details

12-0541-81

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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