Invitrogen

TCR gamma/delta Monoclonal Antibody (eBioGL3 (GL-3, GL3)), Biotin, eBioscience™

TCR gamma/delta Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 5

TCR gamma/delta Antibody (13-5711-82) in ICC/IF

Frontiers in immunology 2020 - Figure 3 Hepatic gammadeltaT cells induce apoptosis of activated HSCs and attenuate liver fibrosis. (A) The primary HSCs were isolated from WT mice and seeded on 24-well plates for 5 days, and then co-cultured with isolated hepatic gammadeltaT cells from CCl 4 -treated WT mice. HSCs were stained with anti-alpha-SMA antibodies (green) and TUNEL (red) to identify apoptotic cells. Nuclei were counterstained wi... View More

TCR gamma/delta Antibody in Flow Cytometry (Flow)

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Product Details

13-5711-82

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.25 µg/test

View 20 publications 20 publications

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioGL3 (GL-3, GL3)

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466668

Product Specific Information

Description: The GL3 monoclonal antibody reacts with the gamma delta T-cell Receptor complex (TCR) on all gamma delta TCR bearing T cells, but it does not react with alpha beta TCR. The gamma delta TCR is present on cells in the thymus, epidermis, epithelial lining of the intestine, peritoneal cavity, and lymphoid tissues.

Applications Reported: This GL3 (GL-3) antibody has been reported for use in flow cytometric analysis.

It is recommended to prestain cells with anti-mouse CD16/32 (Product # 14-0161-85) to prevent non-specific Fc-mediated binding of the GL3 monoclonal antibody.

Applications Tested: This GL3 (GL-3) antibody has been tested by flow cytometric analysis of mouse lymph node cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TCR gamma/delta (T-cell receptor gamma/delta) are specialized T-cells in the immune system. The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory auto-destructive reactions and are believed to be a method by which the immune system compensates for disease. Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). However, a small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Nociceptive sensory neurons promote CD8 T cell responses to HSV-1 infection.

Nature communications

Filtjens J,Roger A,Quatrini L,Wieduwild E,Gouilly J,Hoeffel G,Rossignol R,Daher C,Debroas G,Henri S,Jones CM,Malissen B,Mackay LK,Moqrich A,Carbone FR,Ugolini S

Published figure using TCR gamma/delta monoclonal antibody (Product # 13-5711-82) in Flow Cytometry

Tue May 18 00:00:00 EDT 2021

The mitochondrial iron transporter ABCB7 is required for B cell development, proliferation, and class switch recombination in mice.

eLife

Lehrke MJ,Shapiro MJ,Rajcula MJ,Kennedy MM,McCue SA,Medina KL,Shapiro VS

13-5711-82 was used in Functional assays to demonstrate that ABCB7 is required for bone marrow B cell development, proliferation, and class switch recombination.

Thu Nov 11 00:00:00 EST 2021

Notch2 complements Notch1 to mediate inductive signaling that initiates early T cell development.

The Journal of cell biology

Romero-Wolf M,Shin B,Zhou W,Koizumi M,Rothenberg EV,Hosokawa H

13-5711 was used in Flow cytometry/Cell sorting to define a distinct mechanism of Notch signal response that distinguishes the initial stages of murine T cell development.

Mon Oct 05 00:00:00 EDT 2020

γδT Cells Suppress Liver Fibrosis via Strong Cytolysis and Enhanced NK Cell-Mediated Cytotoxicity Against Hepatic Stellate Cells.

Frontiers in immunology

Liu M,Hu Y,Yuan Y,Tian Z,Zhang C

Published figure using TCR gamma/delta monoclonal antibody (Product # 13-5711-82) in Immunocytochemistry

Wed Sep 02 00:00:00 EDT 2020

TCF1 and LEF1 Control Treg Competitive Survival and Tfr Development to Prevent Autoimmune Diseases.

Cell reports

Yang BH,Wang K,Wan S,Liang Y,Yuan X,Dong Y,Cho S,Xu W,Jepsen K,Feng GS,Lu LF,Xue HH,Fu W

13-5711-82 was used in Flow Cytometry to study how CD4+ Foxp3+ T regulatory (Treg) cell differentiation and functional specification is regulated.

Tue Jun 18 00:00:00 EDT 2019

Costimulation of type-2 innate lymphoid cells by GITR promotes effector function and ameliorates type 2 diabetes.

Nature communications

Galle-Treger L,Sankaranarayanan I,Hurrell BP,Howard E,Lo R,Maazi H,Lewis G,Banie H,Epstein AL,Hu P,Rehan VK,Gilliland FD,Allayee H,Soroosh P,Sharpe AH,Akbari O

13-5711-82 was used in Flow Cytometry to show that GITR, a member of the TNF superfamily, is expressed on both murine and human Type-2 innate lymphoid cells.

Tue Feb 12 00:00:00 EST 2019

TNFR2 Signaling Enhances ILC2 Survival, Function, and Induction of Airway Hyperreactivity.

Cell reports

Hurrell BP,Galle-Treger L,Jahani PS,Howard E,Helou DG,Banie H,Soroosh P,Akbari O

13-5711 was used in Flow cytometry/Cell sorting to investigate the role of TNF/TNFR2 axis in pulmonary ILC2s for possible use in treating ILC2-dependent asthma, in murine and human subjects.

Tue Dec 24 00:00:00 EST 2019

Modulation of autoimmune pathogenesis by T cell-triggered inflammatory cell death.

Nature communications

Sasaki K,Himeno A,Nakagawa T,Sasaki Y,Kiyonari H,Iwai K

13-5711-82 was used in Flow Cytometry to show that T cell-mediated killing and sequential autoinflammation are common and crucial for pathogenic diversity during T cell-mediated autoimmune responses.

Wed Aug 28 00:00:00 EDT 2019

Interleukin-17-Producing γδ T Cells Originate from SOX13⁺ Progenitors that Are Independent of γδTCR Signaling.

Immunity

Spidale NA,Sylvia K,Narayan K,Miu B,Frascoli M,Melichar HJ,Zhihao W,Kisielow J,Palin A,Serwold T,Love P,Kobayashi M,Yoshimoto M,Jain N,Kang J

13-5711-82 was used in Flow Cytometry to generate reporter mice for the Tγδ17 lineage-defining transcription factor SOX13 and identify fetal-origin, intrathymic Sox13+ progenitors.

Tue Nov 20 00:00:00 EST 2018

Transformation of Accessible Chromatin and 3D Nucleome Underlies Lineage Commitment of Early T Cells.

Immunity

Hu G,Cui K,Fang D,Hirose S,Wang X,Wangsa D,Jin W,Ried T,Liu P,Zhu J,Rothenberg EV,Zhao K

13-5711 was used in Flow cytometry/Cell sorting to find that abrupt genome-wide changes at all three levels of chromatin organization occur during the transition from double-negative stage 2 (DN2) to DN3, accompanying the T lineage commitment.

Tue Feb 20 00:00:00 EST 2018

Protective role of γδ T cells in cigarette smoke and influenza infection.

Mucosal immunology

Hong MJ,Gu BH,Madison MC,Landers C,Tung HY,Kim M,Yuan X,You R,Machado AA,Gilbert BE,Soroosh P,Elloso M,Song L,Chen M,Corry DB,Diehl G,Kheradmand F

Published figure using TCR gamma/delta monoclonal antibody (Product # 13-5711-82) in Flow Cytometry

Tue May 01 00:00:00 EDT 2018

A stochastic epigenetic switch controls the dynamics of T-cell lineage commitment.

eLife

Ng KK,Yui MA,Mehta A,Siu S,Irwin B,Pease S,Hirose S,Elowitz MB,Rothenberg EV,Kueh HY

13-5711-82 was used in Flow Cytometry to study the activation of Bcl11b, which controls T-cell fate commitment.

Tue Nov 20 00:00:00 EST 2018

Acidic chitinase primes the protective immune response to gastrointestinal nematodes.

Nature immunology

Vannella KM,Ramalingam TR,Hart KM,de Queiroz Prado R,Sciurba J,Barron L,Borthwick LA,Smith AD,Mentink-Kane M,White S,Thompson RW,Cheever AW,Bock K,Moore I,Fitz LJ,Urban JF,Wynn TA

13-5711 was used in Flow cytometry/Cell sorting to use AMCase-deficient mice as a means to dissect the role of the enzyme in several models of helminth infection and type 2 cytokine-driven airway inflammation.

Sun May 01 00:00:00 EDT 2016

Premature thymic involution is independent of structural plasticity of the thymic stroma.

European journal of immunology

Franckaert D,Schlenner SM,Heirman N,Gill J,Skogberg G,Ekwall O,Put K,Linterman MA,Dooley J,Liston A

13-5711 was used in Flow cytometry/Cell sorting to investigate thymic involution in FVB/N mice, showing that disruption of the epithelial-endothelial relationship and a progressive loss of pro-T cells precedes involution, and loss of pro-T cells is sufficient to drive premature involution, suggesting that pro-T cells are the main driver of involution.

Fri May 01 00:00:00 EDT 2015

IL-1 receptor antagonist-deficient mice develop autoimmune arthritis due to intrinsic activation of IL-17-producing CCR2(+)Vγ6(+)γδ T cells.

Nature communications

Akitsu A,Ishigame H,Kakuta S,Chung SH,Ikeda S,Shimizu K,Kubo S,Liu Y,Umemura M,Matsuzaki G,Yoshikai Y,Saijo S,Iwakura Y

13-5711 was used in Flow cytometry/Cell sorting to investigate the role of IL-17-producing γδ T cells in the development of arthritis in IL-1Ra-deficient mice.

Thu Jun 25 00:00:00 EDT 2015

Expression of a Chimeric Antigen Receptor in Multiple Leukocyte Lineages in Transgenic Mice.

PloS one

Yong CS,Westwood JA,Schröder J,Papenfuss AT,von Scheidt B,Moeller M,Devaud C,Darcy PK,Kershaw MH

13-5711 was used in Flow cytometry/Cell sorting to generate a mouse model expressing chimeric antigen receptors (CARs) specific for a tumour antigen under a pan-hematopoietic promoter.

Wed Jun 08 00:00:00 EDT 2016

CCR10 regulates balanced maintenance and function of resident regulatory and effector T cells to promote immune homeostasis in the skin.

The Journal of allergy and clinical immunology

Xia M,Hu S,Fu Y,Jin W,Yi Q,Matsui Y,Yang J,McDowell MA,Sarkar S,Kalia V,Xiong N

13-5711 was used in Flow cytometry/Cell sorting to investigate the functional importance of CCR10/CCL27 in vivo, showing that CCR10 regulates maintenance and function of regulatory and effector T cells to promote immune homeostasis.

Mon Sep 01 00:00:00 EDT 2014

The alarmin Mrp8/14 as regulator of the adaptive immune response during allergic contact dermatitis.

The EMBO journal

Petersen B,Wolf M,Austermann J,van Lent P,Foell D,Ahlmann M,Kupas V,Loser K,Sorg C,Roth J,Vogl T

13-5711 was used in Magnetic cell separation to study the role of TLR-4 ligand Mrp8/14 in inflammation and adaptive immunity.

Wed Jan 09 00:00:00 EST 2013

CD4 T cells play important roles in maintaining IL-17-producing γδ T-cell subsets in naive animals.

Immunology and cell biology

Do JS,Visperas A,O'Brien RL,Min B

13-5711 was used in Flow cytometry/Cell sorting to investigate the mechanism underlying the regulation of αβ and γδ T-cell homeostasis, showing that CD4 T cells play important roles in maintaining IL-17-producing subsets.

Sun Apr 01 00:00:00 EDT 2012

Dual functions of prostaglandin D2 in murine contact hypersensitivity via DP and CRTH2.

The American journal of pathology

Yamamoto Y,Otani S,Hirai H,Nagata K,Aritake K,Urade Y,Narumiya S,Yokozeki H,Nakamura M,Satoh T

13-5711 was used in Magnetic cell separation to demonstrate that prostaglandin D2 signals through CRTH2 and DP to mediate contact hypersensitivity.

Fri Jul 01 00:00:00 EDT 2011

Wnt4 enhances murine hematopoietic progenitor cell expansion through a planar cell polarity-like pathway.

PloS one

Heinonen KM,Vanegas JR,Lew D,Krosl J,Perreault C

13-5711 was used in Flow cytometry/Cell sorting to establish a functional role for non-canonical Wnt signalling in hematopoiesis through a pathway involving Wnt4, Fz6, Rac1 and Jnk kinases.

Tue Apr 26 00:00:00 EDT 2011

Cutting edge: spontaneous development of IL-17-producing gamma delta T cells in the thymus occurs via a TGF-beta 1-dependent mechanism.

Journal of immunology (Baltimore, Md. : 1950)

Do JS,Fink PJ,Li L,Spolski R,Robinson J,Leonard WJ,Letterio JJ,Min B

13-5711 was used in Flow cytometry/Cell sorting to investigate the mechanisms underlying the generation of gammadelta T cells, showing that it occurs via a TGF-beta 1-dependent mechanism.

Mon Feb 15 00:00:00 EST 2010

Expression of the chemokine binding protein M3 promotes marked changes in the accumulation of specific leukocytes subsets within the intestine.

Gastroenterology

Shang L,Thirunarayanan N,Viejo-Borbolla A,Martin AP,Bogunovic M,Marchesi F,Unkeless JC,Ho Y,Furtado GC,Alcami A,Merad M,Mayer L,Lira SA

Published figure using TCR gamma/delta monoclonal antibody (Product # 13-5711-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2009

In vivo equilibrium of proinflammatory IL-17+ and regulatory IL-10+ Foxp3+ RORgamma t+ T cells.

The Journal of experimental medicine

Lochner M,Peduto L,Cherrier M,Sawa S,Langa F,Varona R,Riethmacher D,Si-Tahar M,Di Santo JP,Eberl G

13-5711 was used in Flow cytometry/Cell sorting to study how potentially antagonistic pro-inflammatory IL-17-producing and regulatory Foxp3(+) RORgamma t(+) T cells coexist and are tightly controlled.

Mon Jun 09 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: T3/TCR complex; TCR delta gamma; TCR gamma/delta; TCR-CD3 complex

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TCR gamma/delta Monoclonal Antibody (eBioGL3 (GL-3, GL3)), Biotin, eBioscience™

TCR gamma/delta Antibody (13-5711-82) in ICC/IF

Product Details

13-5711-82

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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