Invitrogen

TCR gamma/delta Monoclonal Antibody (eBioGL3 (GL-3, GL3)), PerCP-eFluor™ 710, eBioscience™

4 Published Figures

21 References

View all (45) TCR gamma/delta antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (4)

TCR gamma/delta Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 5

TCR gamma/delta Antibody (46-5711-82) in Flow

Staining of C57BL/6 lymph node cells with Anti-Mouse CD3e PE (Product # 12-0031-82) and 0.06 µg of Armenian Hamster IgG Isotype Control PerCP-eFluor® 710 (Product # 46-4888-82) (left) or 0.06 µg of Anti-Mouse gamma/delta TCR PerCP-eFluor® 710 (right). Total viable cells were used for analysis.

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Product Details

46-5711-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 21 publications 21 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), PerCP-eFluor™ 710, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioGL3 (GL-3, GL3)

Conjugate

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

Excitation/Emission Max

482/708 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2016707

Product Specific Information

Description: The GL3 monoclonal antibody reacts with the gamma delta T-cell Receptor complex (TCR) on all gamma delta TCR bearing T cells, but it does not react with alpha beta TCR. The gamma delta TCR is present on cells in the thymus, epidermis, epithelial lining of the intestine, peritoneal cavity, and lymphoid tissues.

Applications Reported: This GL3 (GL-3) antibody has been reported for use in flow cytometric analysis.

It is recommended to prestain cells with anti-mouse CD16/32 (Product # 14-0161-85) to prevent non-specific Fc-mediated binding of the GL3 monoclonal antibody.

Applications Tested: This eBioGL3 (GL-3, GL3) antibody has been tested by flow cytometric analysis of mouse lymph node cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.

Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TCR gamma/delta (T-cell receptor gamma/delta) are specialized T-cells in the immune system. The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory auto-destructive reactions and are believed to be a method by which the immune system compensates for disease. Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). However, a small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Environmental activation of a hypothalamic BDNF-adipocyte IL-15 axis regulates adipose-natural killer cells.

Brain, behavior, and immunity

Bergin SM,Xiao R,Huang W,Judd CRT,Liu X,Mansour AG,Queen N,Widstrom KJ,Caligiuri MA,Cao L

46-5711-82 was used in Flow cytometry/Cell sorting to show that hypothalamic brain-derived neurotrophic factor upregulates IL-15 production in adipocytes via sympathetic β-adrenergic signaling.

Thu Jul 01 00:00:00 EDT 2021

Gut epithelial IL-27 confers intestinal immunity through the induction of intraepithelial lymphocytes.

The Journal of experimental medicine

Lin CH,Chen MC,Lin LL,Christian DA,Min B,Hunter CA,Lu LF

46-5711-82 was used in Flow cytometry/Cell sorting to investigate the role of Gut epithelial IL-27 in mice.

Mon Nov 01 00:00:00 EDT 2021

Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition).

European journal of immunology

Cossarizza A,Chang HD,Radbruch A,Abrignani S,Addo R,Akdis M,Andrä I,Andreata F,Annunziato F,Arranz E,Bacher P,Bari S,Barnaba V,Barros-Martins J,Baumjohann D,Beccaria CG,Bernardo D,Boardman DA,Borger J,Böttcher C,Brockmann L,Burns M,Busch DH,Cameron G,Cammarata I,Cassotta A,Chang Y,Chirdo FG,Christakou E,Čičin-Šain L,Cook L,Corbett AJ,Cornelis R,Cosmi L,Davey MS,De Biasi S,De Simone G,Del Zotto G,Delacher M,Di Rosa F,Di Santo J,Diefenbach A,Dong J,Dörner T,Dress RJ,Dutertre CA,Eckle SBG,Eede P,Evrard M,Falk CS,Feuerer M,Fillatreau S,Fiz-Lopez A,Follo M,Foulds GA,Fröbel J,Gagliani N,Galletti G,Gangaev A,Garbi N,Garrote JA,Geginat J,Gherardin NA,Gibellini L,Ginhoux F,Godfrey DI,Gruarin P,Haftmann C,Hansmann L,Harpur CM,Hayday AC,Heine G,Hernández DC,Herrmann M,Hoelsken O,Huang Q,Huber S,Huber JE,Huehn J,Hundemer M,Hwang WYK,Iannacone M,Ivison SM,Jäck HM,Jani PK,Keller B,Kessler N,Ketelaars S,Knop L,Knopf J,Koay HF,Kobow K,Kriegsmann K,Kristyanto H,Krueger A,Kuehne JF,Kunze-Schumacher H,Kvistborg P,Kwok I,Latorre D,Lenz D,Levings MK,Lino AC,Liotta F,Long HM,Lugli E,MacDonald KN,Maggi L,Maini MK,Mair F,Manta C,Manz RA,Mashreghi MF,Mazzoni A,McCluskey J,Mei HE,Melchers F,Melzer S,Mielenz D,Monin L,Moretta L,Multhoff G,Muñoz LE,Muñoz-Ruiz M,Muscate F,Natalini A,Neumann K,Ng LG,Niedobitek A,Niemz J,Almeida LN,Notarbartolo S,Ostendorf L,Pallett LJ,Patel AA,Percin GI,Peruzzi G,Pinti M,Pockley AG,Pracht K,Prinz I,Pujol-Autonell I,Pulvirenti N,Quatrini L,Quinn KM,Radbruch H,Rhys H,Rodrigo MB,Romagnani C,Saggau C,Sakaguchi S,Sallusto F,Sanderink L,Sandrock I,Schauer C,Scheffold A,Scherer HU,Schiemann M,Schildberg FA,Schober K,Schoen J,Schuh W,Schüler T,Schulz AR,Schulz S,Schulze J,Simonetti S,Singh J,Sitnik KM,Stark R,Starossom S,Stehle C,Szelinski F,Tan L,Tarnok A,Tornack J,Tree TIM,van Beek JJP,van de Veen W,van Gisbergen K,Vasco C,Verheyden NA,von Borstel A,Ward-Hartstonge KA,Warnatz K,Waskow C,Wiedemann A,Wilharm A,Wing J,Wirz O,Wittner J,Yang JHM,Yang J

46-5711-82 was used in Flow cytometry/Cell sorting to provide the key aspects to consider when performing flow cytometry experiments.

Wed Dec 01 00:00:00 EST 2021

Nociceptive sensory neurons promote CD8 T cell responses to HSV-1 infection.

Nature communications

Filtjens J,Roger A,Quatrini L,Wieduwild E,Gouilly J,Hoeffel G,Rossignol R,Daher C,Debroas G,Henri S,Jones CM,Malissen B,Mackay LK,Moqrich A,Carbone FR,Ugolini S

Published figure using TCR gamma/delta monoclonal antibody (Product # 46-5711-82) in Flow Cytometry

Tue May 18 00:00:00 EDT 2021

Neutrophils Driving Unconventional T Cells Mediate Resistance against Murine Sarcomas and Selected Human Tumors.

Cell

Ponzetta A,Carriero R,Carnevale S,Barbagallo M,Molgora M,Perucchini C,Magrini E,Gianni F,Kunderfranco P,Polentarutti N,Pasqualini F,Di Marco S,Supino D,Peano C,Cananzi F,Colombo P,Pilotti S,Alomar SY,Bonavita E,Galdiero MR,Garlanda C,Mantovani A,Jaillon S

46-5711-82 was used in Flow Cytometry to conclude that neutrophils driving UTCαβ polarization and type 1 immunity are essential for resistance against murine sarcomas and selected human tumors.

Thu Jul 11 00:00:00 EDT 2019

Characterization of Transcriptional Regulatory Networks that Promote and Restrict Identities and Functions of Intestinal Innate Lymphoid Cells.

Immunity

Pokrovskii M,Hall JA,Ochayon DE,Yi R,Chaimowitz NS,Seelamneni H,Carriero N,Watters A,Waggoner SN,Littman DR,Bonneau R,Miraldi ER

46-5711 was used in Flow cytometry/Cell sorting to study regulatory interactions between transcription factors and genes within intestinal type 1, 2, and 3 innate lymphoid cells subsets.

Tue Jul 16 00:00:00 EDT 2019

ImmGen report: sexual dimorphism in the immune system transcriptome.

Nature communications

Gal-Oz ST,Maier B,Yoshida H,Seddu K,Elbaz N,Czysz C,Zuk O,Stranger BE,Ner-Gaon H,Shay T

46-5711 was used in Flow cytometry/Cell sorting to show transcriptional sexual dimorphism in macrophages.

Fri Sep 20 00:00:00 EDT 2019

Antibody-Mediated Delivery of VEGFC Ameliorates Experimental Chronic Colitis.

ACS pharmacology & translational science

Tacconi C,Schwager S,Cousin N,Bajic D,Sesartic M,Sundberg JP,Neri D,Detmar M

46-5711-82 was used in Flow Cytometry to investigate whether targeted delivery of the pro-lymphangiogenic vascular endothelial growth factor C to the site of inflammation may represent a new, clinically feasible strategy for treating inflammatory bowel disease.

Fri Oct 11 00:00:00 EDT 2019

Interleukin-17-Producing γδ T Cells Originate from SOX13⁺ Progenitors that Are Independent of γδTCR Signaling.

Immunity

Spidale NA,Sylvia K,Narayan K,Miu B,Frascoli M,Melichar HJ,Zhihao W,Kisielow J,Palin A,Serwold T,Love P,Kobayashi M,Yoshimoto M,Jain N,Kang J

46-5711-82 was used in Flow Cytometry to generate reporter mice for the Tγδ17 lineage-defining transcription factor SOX13 and identify fetal-origin, intrathymic Sox13+ progenitors.

Tue Nov 20 00:00:00 EST 2018

Protective role of γδ T cells in cigarette smoke and influenza infection.

Mucosal immunology

Hong MJ,Gu BH,Madison MC,Landers C,Tung HY,Kim M,Yuan X,You R,Machado AA,Gilbert BE,Soroosh P,Elloso M,Song L,Chen M,Corry DB,Diehl G,Kheradmand F

Published figure using TCR gamma/delta monoclonal antibody (Product # 46-5711-82) in Flow Cytometry

Tue May 01 00:00:00 EDT 2018

Stabilization of cytokine mRNAs in iNKT cells requires the serine-threonine kinase IRE1alpha.

Nature communications

Govindarajan S,Gaublomme D,Van der Cruyssen R,Verheugen E,Van Gassen S,Saeys Y,Tavernier S,Iwawaki T,Bloch Y,Savvides SN,Lambrecht BN,Janssens S,Elewaut D,Drennan MB

46-5711 was used in Flow cytometry/Cell sorting to examine the role of the IRE1α-dependent signalling cascade in coupling constitutive cytokine mRNA expression to the rapid induction of cytokine secretion and effector functions in activated iNKT cells.

Mon Dec 17 00:00:00 EST 2018

Intestinal Epithelial and Intraepithelial T Cell Crosstalk Mediates a Dynamic Response to Infection.

Cell

Hoytema van Konijnenburg DP,Reis BS,Pedicord VA,Farache J,Victora GD,Mucida D

46-5711 was used in Flow cytometry/Cell sorting to investigate the nature of intestinal intraepithelial lymphocyte responses to luminal microbes.

Thu Nov 02 00:00:00 EDT 2017

Guidelines for the use of flow cytometry and cell sorting in immunological studies.

European journal of immunology

Cossarizza A,Chang HD,Radbruch A,Akdis M,Andrä I,Annunziato F,Bacher P,Barnaba V,Battistini L,Bauer WM,Baumgart S,Becher B,Beisker W,Berek C,Blanco A,Borsellino G,Boulais PE,Brinkman RR,Büscher M,Busch DH,Bushnell TP,Cao X,Cavani A,Chattopadhyay PK,Cheng Q,Chow S,Clerici M,Cooke A,Cosma A,Cosmi L,Cumano A,Dang VD,Davies D,De Biasi S,Del Zotto G,Della Bella S,Dellabona P,Deniz G,Dessing M,Diefenbach A,Di Santo J,Dieli F,Dolf A,Donnenberg VS,Dörner T,Ehrhardt GRA,Endl E,Engel P,Engelhardt B,Esser C,Everts B,Dreher A,Falk CS,Fehniger TA,Filby A,Fillatreau S,Follo M,Förster I,Foster J,Foulds GA,Frenette PS,Galbraith D,Garbi N,García-Godoy MD,Geginat J,Ghoreschi K,Gibellini L,Goettlinger C,Goodyear CS,Gori A,Grogan J,Gross M,Grützkau A,Grummitt D,Hahn J,Hammer Q,Hauser AE,Haviland DL,Hedley D,Herrera G,Herrmann M,Hiepe F,Holland T,Hombrink P,Houston JP,Hoyer BF,Huang B,Hunter CA,Iannone A,Jäck HM,Jávega B,Jonjic S,Juelke K,Jung S,Kaiser T,Kalina T,Keller B,Khan S,Kienhöfer D,Kroneis T,Kunkel D,Kurts C,Kvistborg P,Lannigan J,Lantz O,Larbi A,LeibundGut-Landmann S,Leipold MD,Levings MK,Litwin V,Liu Y,Lohoff M,Lombardi G,Lopez L,Lovett-Racke A,Lubberts E,Ludewig B,Lugli E,Maecker HT,Martrus G,Matarese G,Maueröder C,McGrath M,McInnes I,Mei HE,Melchers F,Melzer S,Mielenz D,Mills K,Mirrer D,Mjösberg J,Moore J,Moran B,Moretta A,Moretta L,Mosmann TR,Müller S,Müller W,Münz C,Multhoff G,Munoz LE,Murphy KM,Nakayama T,Nasi M,Neudörfl C,Nolan J,Nourshargh S,O'Connor JE,Ouyang W,Oxenius A,Palankar R,Panse I,Peterson P,Peth C,Petriz J,Philips D,Pickl W,Piconese S,Pinti M,Pockley AG,Podolska MJ,Pucillo C,Quataert SA,Radstake TRDJ,Rajwa B,Rebhahn JA,Recktenwald D,Remmerswaal EBM,Rezvani K,Rico LG,Robinson JP,Romagnani C,Rubartelli A,Ruckert B,Ruland J,Sakaguchi S,Sala-de-Oyanguren F,Samstag Y,Sanderson S,Sawitzki B,Scheffold A,Schiemann M,Schildberg F,Schimisky E,Schmid SA,Schmitt S,Schober K,Schüler T,Schulz AR,Schumacher T,Scotta C,Shankey TV,Shemer A,Simon AK,Spidlen J,Stall AM,Stark R,Stehle C,Stein M,Steinmetz T,Stockinger H,Takahama Y,Tarnok A,Tian Z,Toldi G,Tornack J,Traggiai E,Trotter J,Ulrich H,van der Braber M,van Lier RAW,Veldhoen M,Vento-Asturias S,Vieira P,Voehringer D,Volk HD,von Volkmann K,Waisman A,Walker R,Ward MD,Warnatz K,Warth S,Watson JV,Watzl C,Wegener L,Wiedemann A,Wienands J,Willimsky G,Wing J,Wurst P,Yu L,Yue A,Zhang Q,Zhao Y,Ziegler S,Zimmermann J

46-5711 was used in Flow cytometry/Cell sorting to collect the currently accepted best methods for monitoring most of the variation of the major players of immune system.

Sun Oct 01 00:00:00 EDT 2017

Exploiting a host-commensal interaction to promote intestinal barrier function and enteric pathogen tolerance.

Science immunology

Pedicord VA,Lockhart AAK,Rangan KJ,Craig JW,Loschko J,Rogoz A,Hang HC,Mucida D

46-5711 was used in Flow cytometry/Cell sorting to demonstrate that specific factors from commensal bacteria can improve host barrier function and limit the pathogenesis of distinct enteric infections.

Thu Sep 01 00:00:00 EDT 2016

NKT sublineage specification and survival requires the ubiquitin-modifying enzyme TNFAIP3/A20.

The Journal of experimental medicine

Drennan MB,Govindarajan S,Verheugen E,Coquet JM,Staal J,McGuire C,Taghon T,Leclercq G,Beyaert R,van Loo G,Lambrecht BN,Elewaut D

46-5711 was used in Flow cytometry/Cell sorting to show that negative regulation of TCR signalling during NKT development controls the differentiation and survival of NKT1 and NKT2 cells.

Mon Sep 19 00:00:00 EDT 2016

Control of murine cytomegalovirus infection by γδ T cells.

PLoS pathogens

Sell S,Dietz M,Schneider A,Holtappels R,Mach M,Winkler TH

46-5711 was used in Flow cytometry/Cell sorting to show that γδ T cells can provide an effective control mechanism of acute cytomegalovirus infections.

Sun Feb 01 00:00:00 EST 2015

A genome-wide analysis identifies a notch-RBP-Jκ-IL-7Rα axis that controls IL-17-producing γδ T cell homeostasis in mice.

Journal of immunology (Baltimore, Md. : 1950)

Nakamura M,Shibata K,Hatano S,Sato T,Ohkawa Y,Yamada H,Ikuta K,Yoshikai Y

46-5711 was used in Flow cytometry/Cell sorting to investigate additional roles for the Notch axis in the homeostasis of γδ T cells.

Thu Jan 01 00:00:00 EST 2015

IL-1 receptor antagonist-deficient mice develop autoimmune arthritis due to intrinsic activation of IL-17-producing CCR2(+)Vγ6(+)γδ T cells.

Nature communications

Akitsu A,Ishigame H,Kakuta S,Chung SH,Ikeda S,Shimizu K,Kubo S,Liu Y,Umemura M,Matsuzaki G,Yoshikai Y,Saijo S,Iwakura Y

Published figure using TCR gamma/delta monoclonal antibody (Product # 46-5711-82) in Flow Cytometry

Thu Jun 25 00:00:00 EDT 2015

IFN-γ-producing and IL-17-producing γδ T cells differentiate at distinct developmental stages in murine fetal thymus.

Journal of immunology (Baltimore, Md. : 1950)

Shibata K,Yamada H,Nakamura M,Hatano S,Katsuragi Y,Kominami R,Yoshikai Y

46-5711 was used in Flow cytometry/Cell sorting to compare the functions of γδ T cells derived from double-negative 2 and double-negative 3 cells in the murine fetal thymus.

Sat Mar 01 00:00:00 EST 2014

Epidermal ablation of Dlx3 is linked to IL-17-associated skin inflammation.

Proceedings of the National Academy of Sciences of the United States of America

Hwang J,Kita R,Kwon HS,Choi EH,Lee SH,Udey MC,Morasso MI

46-5711 was used in Flow cytometry/Cell sorting to investigate the role of Distal-less 3 (Dlx3) in cutaneous biology and pathophysiology, showing that ablation is linked to IL-17-associated skin inflammation.

Tue Jul 12 00:00:00 EDT 2011

Expression of the chemokine binding protein M3 promotes marked changes in the accumulation of specific leukocytes subsets within the intestine.

Gastroenterology

Shang L,Thirunarayanan N,Viejo-Borbolla A,Martin AP,Bogunovic M,Marchesi F,Unkeless JC,Ho Y,Furtado GC,Alcami A,Merad M,Mayer L,Lira SA

Published figure using TCR gamma/delta monoclonal antibody (Product # 46-5711-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: T3/TCR complex; TCR delta gamma; TCR gamma/delta; TCR-CD3 complex

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TCR gamma/delta Monoclonal Antibody (eBioGL3 (GL-3, GL3)), PerCP-eFluor™ 710, eBioscience™

TCR gamma/delta Antibody (46-5711-82) in Flow

Product Details

46-5711-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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