Invitrogen

TLR4/MD-2 Complex Monoclonal Antibody (MTS510), Biotin, eBioscience™

23 Published Figures

22 References

View all (10) TLR4/MD-2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (23)

TLR4/MD-2 Complex Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 24

TLR4/MD-2 Complex Antibody (13-9924-81) in Flow

Staining of 3 day thioglycolate-induced peritoneal macrophages with 0.25 µg of Rat IgG2a Isotype Control Biotin (Product # 13-4321-82) (open histogram) or 0.25 µg of Anti-Mouse CD284 (TLR4) Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Cells were pre-incubated with Anti-Mouse CD16/32 Purified (Product # 14-0161-82) to block Fc binding. Total viable cells were used for analysi... View More

TLR4/MD-2 Complex Antibody in Western Blot (WB)

TLR4/MD-2 Complex Antibody in Immunocytochemistry (ICC/IF)

TLR4/MD-2 Complex Antibody in Immunohistochemistry (IHC)

TLR4/MD-2 Complex Antibody in Neutralization (Neu)

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Product Details

13-9924-81

Applications

Tested Dilution

Publications

Western Blot (WB)

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

0.5 µg/test

View 11 publications 11 publications

Neutralization (Neu)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MTS510

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466991

Product Specific Information

Description: The MTS510 monoclonal antibody reacts with the mouse Toll-like receptor 4 (TLR4)/MD-2 complex. At least ten members of the Toll family have been identified. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR4 physically associates with MD-2, and together with CD14, this complex is responsible for LPS recognition and signaling. In the mouse, TLR4 is expressed by thioglycolate-elicited peritoneal macrophages. Incubation of peritoneal macrophages with LPS results in down regulation of surface TLR4/MD-2. The TLR4 gene is defective in C3H/HeJ and C57BL/10ScCr mice, both of which have been well characterized as hyporesponders to LPS.

The MTS510 monoclonal antibody co-immunoprecipitates MD-2 (~30 kDa) and TLR4 (~100 kDa), and preferentially reacts with TLR4 that is associated with MD-2. In comparison, binding of the UT41 monoclonal antibody occurs with and without formation of the TLR4/MD-2 complex. Please contact eBioscience Technical Support for further information.

Applications Reported: MTS510 has been reported for use in flow cytometric analysis and immunohistochemical staining of frozen tissue sections.

Applications Tested: The MTS510 antibody has been tested by flow cytometric analysis of mouse thioglycolate-elicited peritoneal exudate cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

MD-2 is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses. TLR4, the major signaling receptor for lipopolysaccharide (LPS), requires the binding of MD-2 to its extracellular region for maximal response to LPS. The specificity of this response is determined by the species of MD-2; e.g., human MD-2 transfected into mouse cells can cause mouse TLR4 to react to LPS analogs that are normally antagonistic to human but not mouse TLR4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Hydroxychloroquine attenuates neuroinflammation following traumatic brain injury by regulating the TLR4/NF-κB signaling pathway.

Journal of neuroinflammation

Hu J,Wang X,Chen X,Fang Y,Chen K,Peng W,Wang Z,Guo K,Tan X,Liang F,Lin L,Xiong Y

Published figure using TLR4/MD-2 Complex monoclonal antibody (Product # 13-9924-81) in Western Blot

Mon Mar 28 00:00:00 EDT 2022

Lycopene Inhibits Toll-Like Receptor 4-Mediated Expression of Inflammatory Cytokines in House Dust Mite-Stimulated Respiratory Epithelial Cells.

Molecules (Basel, Switzerland)

Choi J,Lim JW,Kim H

Published figure using TLR4/MD-2 Complex monoclonal antibody (Product # 13-9924-81) in Immunocytochemistry

Mon May 24 00:00:00 EDT 2021

Inflammatory signaling in dengue-infected platelets requires translation and secretion of nonstructural protein 1.

Blood advances

Quirino-Teixeira AC,Rozini SV,Barbosa-Lima G,Coelho DR,Carneiro PH,Mohana-Borges R,Bozza PT,Hottz ED

Published figure using TLR4/MD-2 Complex monoclonal antibody (Product # 13-9924-81) in Neutralization

Tue May 12 00:00:00 EDT 2020

Chanzyme TRPM7 Mediates the Ca²⁺ Influx Essential for Lipopolysaccharide-Induced Toll-Like Receptor 4 Endocytosis and Macrophage Activation.

Immunity

Schappe MS,Szteyn K,Stremska ME,Mendu SK,Downs TK,Seegren PV,Mahoney MA,Dixit S,Krupa JK,Stipes EJ,Rogers JS,Adamson SE,Leitinger N,Desai BN

Published figure using TLR4/MD-2 Complex monoclonal antibody (Product # 13-9924-81) in Flow Cytometry

Tue Jan 16 00:00:00 EST 2018

Alarmin S100A8 Activates Alveolar Epithelial Cells in the Context of Acute Lung Injury in a TLR4-Dependent Manner.

Frontiers in immunology

Chakraborty D,Zenker S,Rossaint J,Hölscher A,Pohlen M,Zarbock A,Roth J,Vogl T

13-9924 was used in Immunocytochemistry-immunoflourescence to show that S100A8 promotes acute lung injury via Toll-like receptor 4-dependent activation of AECs.

Sun Jan 03 00:00:00 EST 2021

High mobility group box 1 induces calcification of aortic valve interstitial cells via toll-like receptor 4.

Molecular medicine reports

Shen W,Zhou J,Wang C,Xu G,Wu Y,Hu Z

Published figure using TLR4/MD-2 Complex monoclonal antibody (Product # 13-9924-81) in Neutralization

Mon May 01 00:00:00 EDT 2017

Green Tea Polyphenol Epigallocatechin-3-gallate Suppresses Toll-like Receptor 4 Expression via Up-regulation of E3 Ubiquitin-protein Ligase RNF216.

The Journal of biological chemistry

Kumazoe M,Nakamura Y,Yamashita M,Suzuki T,Takamatsu K,Huang Y,Bae J,Yamashita S,Murata M,Yamada S,Shinoda Y,Yamaguchi W,Toyoda Y,Tachibana H

Published figure using TLR4/MD-2 Complex monoclonal antibody (Product # 13-9924-81) in Flow Cytometry

Fri Mar 10 00:00:00 EST 2017

Identification of a novel mechanism of blood-brain communication during peripheral inflammation via choroid plexus-derived extracellular vesicles.

EMBO molecular medicine

Balusu S,Van Wonterghem E,De Rycke R,Raemdonck K,Stremersch S,Gevaert K,Brkic M,Demeestere D,Vanhooren V,Hendrix A,Libert C,Vandenbroucke RE

13992481 was used in immunohistochemistry - frozen section to investigate the role of extracellular vesicles by the choroid plexus epithelium in blood-brain communication

Sat Oct 01 00:00:00 EDT 2016

Exploring the Functional Complementation between Grp94 and Hsp90.

PloS one

Maharaj KA,Que NL,Hong F,Huck JD,Gill SK,Wu S,Li Z,Gewirth DT

13-9924 was used in Flow cytometry/Cell sorting to identify the interface between the Middle and C-terminal domains as an important structural unit within the Hsp90 family.

Tue Jun 27 00:00:00 EDT 2017

Chronic exposure to low dose bacterial lipopolysaccharide inhibits leptin signaling in vagal afferent neurons.

Physiology & behavior

de La Serre CB,de Lartigue G,Raybould HE

Published figure using TLR4/MD-2 Complex monoclonal antibody (Product # 13-9924-81) in Immunohistochemistry

Sun Feb 01 00:00:00 EST 2015

Chronic exposure to low dose bacterial lipopolysaccharide inhibits leptin signaling in vagal afferent neurons.

Physiology & behavior

de La Serre CB,de Lartigue G,Raybould HE

Published figure using TLR4/MD-2 Complex monoclonal antibody (Product # 13-9924-81) in Immunohistochemistry

Sun Feb 01 00:00:00 EST 2015

Toll-like receptor 4 mediates inflammatory cytokine secretion in smooth muscle cells induced by oxidized low-density lipoprotein.

PloS one

Yang K,Zhang XJ,Cao LJ,Liu XH,Liu ZH,Wang XQ,Chen QJ,Lu L,Shen WF,Liu Y

Published figure using TLR4/MD-2 Complex monoclonal antibody (Product # 13-9924-81) in Immunofluorescence

Tue Jan 20 00:00:00 EST 2015

Toll-like receptor 4 mediates inflammatory cytokine secretion in smooth muscle cells induced by oxidized low-density lipoprotein.

PloS one

Yang K,Zhang XJ,Cao LJ,Liu XH,Liu ZH,Wang XQ,Chen QJ,Lu L,Shen WF,Liu Y

Published figure using TLR4/MD-2 Complex monoclonal antibody (Product # 13-9924-81) in Immunofluorescence

Tue Jan 20 00:00:00 EST 2015

Serum amyloid A3 binds MD-2 to activate p38 and NF-κB pathways in a MyD88-dependent manner.

Journal of immunology (Baltimore, Md. : 1950)

Deguchi A,Tomita T,Omori T,Komatsu A,Ohto U,Takahashi S,Tanimura N,Akashi-Takamura S,Miyake K,Maru Y

13-9924 was used in Flow cytometry/Cell sorting to investigate how SAA3 directly binds MD-2 and activates the MyD88-dependent TLR4/MD-2 pathway.

Thu Aug 15 00:00:00 EDT 2013

Inefficient TLR4/MD-2 heterotetramerization by monophosphoryl lipid A.

PloS one

Casella CR,Mitchell TC

Published figure using TLR4/MD-2 Complex monoclonal antibody (Product # 13-9924-81) in Flow Cytometry

Mon Nov 11 00:00:00 EST 2013

α7 helix region of αI domain is crucial for integrin binding to endoplasmic reticulum chaperone gp96: a potential therapeutic target for cancer metastasis.

The Journal of biological chemistry

Hong F,Liu B,Chiosis G,Gewirth DT,Li Z

13-9924 was used in Flow cytometry/Cell sorting to demonstrate that the dimerisation of integrin αL and β2 is highly dependent on gp96.

Fri Jun 21 00:00:00 EDT 2013

Defective antiviral responses of induced pluripotent stem cells to baculoviral vector transduction.

Journal of virology

Chen GY,Hwang SM,Su HJ,Kuo CY,Luo WY,Lo KW,Huang CC,Chen CL,Yu SH,Hu YC

Published figure using TLR4/MD-2 Complex monoclonal antibody (Product # 13-9924-81) in Flow Cytometry

Wed Aug 01 00:00:00 EDT 2012

The molecular chaperone gp96/GRP94 interacts with Toll-like receptors and integrins via its C-terminal hydrophobic domain.

The Journal of biological chemistry

Wu S,Hong F,Gewirth D,Guo B,Liu B,Li Z

13-9924 was used in Flow cytometry/Cell sorting to determine how the client-binding domain (CBD) within the gp96/GRP94 molecular chaperone binds to Toll-like receptors (TLRs) and integrins.

Fri Feb 24 00:00:00 EST 2012

Plasmodium yoelii blood-stage primes macrophage-mediated innate immune response through modulation of toll-like receptor signalling.

Malaria journal

Fu Y,Ding Y,Zhou T,Fu X,Xu W

13-9924 was used in Flow cytometry/Cell sorting to study the immune response following infection of a lethal or non-lethal strain of rodent malaria parasites.

Sun Apr 01 00:00:00 EDT 2012

Vav proteins control MyD88-dependent oxidative burst.

Blood

Miletic AV,Graham DB,Montgrain V,Fujikawa K,Kloeppel T,Brim K,Weaver B,Schreiber R,Xavier R,Swat W

13-9924 was used in Flow cytometry/Cell sorting to display that in the context of reactive oxygen intermediates, the Vav family of Rho guanine nucleotide exchange factors (GEFs) mediate a subset a signals emanating from MyD88.

Sun Apr 15 00:00:00 EDT 2007

Toll-like receptors on hematopoietic progenitor cells stimulate innate immune system replenishment.

Immunity

Nagai Y,Garrett KP,Ohta S,Bahrun U,Kouro T,Akira S,Takatsu K,Kincade PW

13-9924 was used in Flow cytometry/Cell sorting to investigate the expression of toll-like receptors on different hematopoietic stem cells and the mechanism by which they stimulate innate immune system replenishment.

Thu Jun 01 00:00:00 EDT 2006

TLRs regulate the gatekeeping functions of the intestinal follicle-associated epithelium.

Journal of immunology (Baltimore, Md. : 1950)

Chabot S,Wagner JS,Farrant S,Neutra MR

Sat Apr 01 00:00:00 EST 2006

Bioinformatics

Protein Aliases: ARMD10; CD284; ESOP-1; lipopolysaccharide response; Ly-96; Lymphocyte antigen 96; myeloid differentiation factor-2; myeloid differentiation protein-2; Protein MD-2; TLR-4; TOLL; Toll-like receptor 4

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Gene Aliases: ESOP-1; Esop1; Lps; Ly87; Ly96; MD-2; MD2; Ran/M1; Rasl2-8; Tlr4

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UniProt ID: (Mouse) Q9JHF9, (Mouse) Q9QUK6

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Entrez Gene ID: (Mouse) 17087, (Mouse) 21898

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Function(s)

lipopolysaccharide receptor activity protein binding lipopolysaccharide binding receptor activity transmembrane signaling receptor activity phosphatidylinositol 3-kinase binding scaffold/adaptor protein

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TLR4/MD-2 Complex Monoclonal Antibody (MTS510), Biotin, eBioscience™

TLR4/MD-2 Complex Antibody (13-9924-81) in Flow

Product Details

13-9924-81

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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