Product References
CD206+ macrophages transventricularly infiltrate the early embryonic cerebral wall to differentiate into microglia.
Cell reports
Hattori Y,Kato D,Murayama F,Koike S,Asai H,Yamasaki A,Naito Y,Kawaguchi A,Konishi H,Prinz M,Masuda T,Wake H,Miyata T
14-0443-82 was used in Immunocytochemistry-immunofluorescence to demonstrate that the "roof plate?ventricle?pallium" route is an essential path for microglial colonization into the embryonic mouse brain.
Tue Feb 28 00:00:00 EST 2023
CD206+ macrophages transventricularly infiltrate the early embryonic cerebral wall to differentiate into microglia.
Cell reports
Hattori Y,Kato D,Murayama F,Koike S,Asai H,Yamasaki A,Naito Y,Kawaguchi A,Konishi H,Prinz M,Masuda T,Wake H,Miyata T
14-0443-82 was used in Immunocytochemistry-immunofluorescence to demonstrate that the "roof plate?ventricle?pallium" route is an essential path for microglial colonization into the embryonic mouse brain.
Tue Feb 28 00:00:00 EST 2023
Bacterial meningitis in the early postnatal mouse studied at single-cell resolution.
eLife
Wang J,Rattner A,Nathans J
14-0443-82 was used in Immunohistochemistry to imply that EC responses to infection are largely driven by the intrinsic EC response to LPS.
Thu Jun 15 00:00:00 EDT 2023
Activating SIRT3 in peritoneal mesothelial cells alleviates postsurgical peritoneal adhesion formation by decreasing oxidative stress and inhibiting the NLRP3 inflammasome.
Experimental & molecular medicine
Shen T,Wu Y,Wang X,Wang Z,Li E,Zhou C,Yue C,Jiang Z,Wei G,Lian J,Xu Q,Li X
14-0443-82 was used in Flow Cytometry to show that the rescue of SIRT3 by HKL may be a new therapeutic strategy to alleviate and block postsurgical PA formation.
Thu Sep 01 00:00:00 EDT 2022
Immuno-localization of definitive hematopoietic stem cells in the vascular niche of mouse fetal liver.
STAR protocols
Biswas A,Singh SK,Kartha GM,Khurana S
Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Immunohistochemistry
Fri Dec 16 00:00:00 EST 2022
Deciphering the heterogeneity of the Lyve1+ perivascular macrophages in the mouse brain.
Nature communications
Siret C,van Lessen M,Bavais J,Jeong HW,Reddy Samawar SK,Kapupara K,Wang S,Simic M,de Fabritus L,Tchoghandjian A,Fallet M,Huang H,Sarrazin S,Sieweke MH,Stumm R,Sorokin L,Adams RH,Schulte-Merker S,Kiefer F,van de Pavert SA
Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Flow Cytometry
Wed Nov 30 00:00:00 EST 2022
Absence of microglia promotes diverse pathologies and early lethality in Alzheimer's disease mice.
Cell reports
Kiani Shabestari S,Morabito S,Danhash EP,McQuade A,Sanchez JR,Miyoshi E,Chadarevian JP,Claes C,Coburn MA,Hasselmann J,Hidalgo J,Tran KN,Martini AC,Chang Rothermich W,Pascual J,Head E,Hume DA,Pridans C,Davtyan H,Swarup V,Blurton-Jones M
14-0443-82 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen) to indicate the protective functions of microglia in reducing CAA, blood-brain barrier dysfunction, and brain calcification.
Tue Jun 14 00:00:00 EDT 2022
The E3 ubiquitin ligase WWP2 regulates pro-fibrogenic monocyte infiltration and activity in heart fibrosis.
Nature communications
Chen H,Chew G,Devapragash N,Loh JZ,Huang KY,Guo J,Liu S,Tan ELS,Chen S,Tee NGZ,Mia MM,Singh MK,Zhang A,Behmoaras J,Petretto E
14-0443-82 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen) to identify a pro-fibrogenic macrophage subtype in non-ischemic cardiomyopathy, and demonstrate that WWP2 is a key regulator of IRF7-mediated Ccl5/Ly6chigh monocyte axis in heart fibrosis.
Wed Nov 30 00:00:00 EST 2022
Integrins mediate placental extracellular vesicle trafficking to lung and liver in vivo.
Scientific reports
Nguyen SL,Ahn SH,Greenberg JW,Collaer BW,Agnew DW,Arora R,Petroff MG
Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Immunohistochemistry
Thu Feb 18 00:00:00 EST 2021
Nasal administration of mitochondria reverses chemotherapy-induced cognitive deficits.
Theranostics
Alexander JF,Seua AV,Arroyo LD,Ray PR,Wangzhou A,Heiβ-Lückemann L,Schedlowski M,Price TJ,Kavelaars A,Heijnen CJ
14-0443-82 was used in Sample Preparation to whether nasal administration of mitochondria isolated from human mesenchymal stem cells to mice, can reverse cisplatin-induced cognitive deficits.
Tue Jul 27 00:00:00 EDT 2021
Nasal administration of mitochondria reverses chemotherapy-induced cognitive deficits.
Theranostics
Alexander JF,Seua AV,Arroyo LD,Ray PR,Wangzhou A,Heiβ-Lückemann L,Schedlowski M,Price TJ,Kavelaars A,Heijnen CJ
14-0443-82 was used in Sample Preparation to whether nasal administration of mitochondria isolated from human mesenchymal stem cells to mice, can reverse cisplatin-induced cognitive deficits.
Tue Jul 27 00:00:00 EDT 2021
Nasal administration of mitochondria reverses chemotherapy-induced cognitive deficits.
Theranostics
Alexander JF,Seua AV,Arroyo LD,Ray PR,Wangzhou A,Heiβ-Lückemann L,Schedlowski M,Price TJ,Kavelaars A,Heijnen CJ
14-0443-82 was used in Sample Preparation to whether nasal administration of mitochondria isolated from human mesenchymal stem cells to mice, can reverse cisplatin-induced cognitive deficits.
Tue Jul 27 00:00:00 EDT 2021
Interleukin-11-expressing fibroblasts have a unique gene signature correlated with poor prognosis of colorectal cancer.
Nature communications
Nishina T,Deguchi Y,Ohshima D,Takeda W,Ohtsuka M,Shichino S,Ueha S,Yamazaki S,Kawauchi M,Nakamura E,Nishiyama C,Kojima Y,Adachi-Akahane S,Hasegawa M,Nakayama M,Oshima M,Yagita H,Shibuya K,Mikami T,Inohara N,Matsushima K,Tada N,Nakano H
Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Flow Cytometry
Fri Apr 16 00:00:00 EDT 2021
Extracellular ATP Limits Homeostatic T Cell Migration Within Lymph Nodes.
Frontiers in immunology
Kobayashi D,Sugiura Y,Umemoto E,Takeda A,Ueta H,Hayasaka H,Matsuzaki S,Katakai T,Suematsu M,Hamachi I,Yegutkin GG,Salmi M,Jalkanen S,Miyasaka M
Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Immunohistochemistry
Mon Feb 14 00:00:00 EST 2022
Targeting local lymphatics to ameliorate heterotopic ossification via FGFR3-BMPR1a pathway.
Nature communications
Zhang D,Huang J,Sun X,Chen H,Huang S,Yang J,Du X,Tan Q,Luo F,Zhang R,Zhou S,Jiang W,Ni Z,Wang Z,Jin M,Xu M,Li F,Chen L,Liu M,Su N,Luo X,Yin L,Zhu Y,Feng JQ,Chen D,Qi H,Chen L,Xie Y
Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Immunocytochemistry
Mon Jul 19 00:00:00 EDT 2021
Lymphocyte access to lymphoma is impaired by high endothelial venule regression.
Cell reports
Menzel L,Zschummel M,Crowley T,Franke V,Grau M,Ulbricht C,Hauser A,Siffrin V,Bajénoff M,Acton SE,Akalin A,Lenz G,Willimsky G,Höpken UE,Rehm A
14-0443-82 was used in Immunohistochemistry to show that lymphoma causes severe high endothelial venule regression in mouse models that phenocopies aggressive human B cell lymphomas.
Tue Oct 26 00:00:00 EDT 2021
SPNS2 enables T cell egress from lymph nodes during an immune response.
Cell reports
Okuniewska M,Fang V,Baeyens A,Raghavan V,Lee JY,Littman DR,Schwab SR
14-0443-82 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen) to demonstrate that SPNS2 supplies the S1P that guides T cells out of LNs with an ongoing immune response.
Tue Jul 13 00:00:00 EDT 2021
Meningeal lymphatic vessels regulate brain tumor drainage and immunity.
Cell research
Hu X,Deng Q,Ma L,Li Q,Chen Y,Liao Y,Zhou F,Zhang C,Shao L,Feng J,He T,Ning W,Kong Y,Huo Y,He A,Liu B,Zhang J,Adams R,He Y,Tang F,Bian X,Luo J
Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Flow Cytometry
Sun Mar 01 00:00:00 EST 2020
Meningeal lymphatic vessels regulate brain tumor drainage and immunity.
Cell research
Hu X,Deng Q,Ma L,Li Q,Chen Y,Liao Y,Zhou F,Zhang C,Shao L,Feng J,He T,Ning W,Kong Y,Huo Y,He A,Liu B,Zhang J,Adams R,He Y,Tang F,Bian X,Luo J
Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Flow Cytometry
Sun Mar 01 00:00:00 EST 2020
An ocular glymphatic clearance system removes β-amyloid from the rodent eye.
Science translational medicine
Wang X,Lou N,Eberhardt A,Yang Y,Kusk P,Xu Q,Förstera B,Peng S,Shi M,Ladrón-de-Guevara A,Delle C,Sigurdsson B,Xavier ALR,Ertürk A,Libby RT,Chen L,Thrane AS,Nedergaard M
14-0443 was used in Immunocytochemistry to identify an ocular glymphatic clearance route for fluid and wastes via the proximal optic nerve in rodents.
Wed Mar 25 00:00:00 EDT 2020
Tissue-resident macrophages in omentum promote metastatic spread of ovarian cancer.
The Journal of experimental medicine
Etzerodt A,Moulin M,Doktor TK,Delfini M,Mossadegh-Keller N,Bajenoff M,Sieweke MH,Moestrup SK,Auphan-Anezin N,Lawrence T
Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Flow Cytometry
Mon Apr 06 00:00:00 EDT 2020
FK506 induces lung lymphatic endothelial cell senescence and downregulates LYVE-1 expression, with associated decreased hyaluronan uptake.
Molecular medicine (Cambridge, Mass.)
Shrestha S,Cho W,Stump B,Imani J,Lamattina AM,Louis PH,Pazzanese J,Rosas IO,Visner G,Perrella MA,El-Chemaly S
Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Flow Cytometry
Fri Jul 31 00:00:00 EDT 2020
Anatomic evidence shows that lymphatic drainage exists in the pituitary to loop the cerebral lymphatic circulation.
Medical hypotheses
Elham E,Wumaier R,Wang C,Luo X,Chen T,Zhong N
Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Immunohistochemistry
Thu Oct 01 00:00:00 EDT 2020
Meningeal lymphatics clear erythrocytes that arise from subarachnoid hemorrhage.
Nature communications
Chen J,Wang L,Xu H,Xing L,Zhuang Z,Zheng Y,Li X,Wang C,Chen S,Guo Z,Liang Q,Wang Y
Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Immunohistochemistry
Mon Jun 22 00:00:00 EDT 2020
Efficient Tissue Clearing and Multi-Organ Volumetric Imaging Enable Quantitative Visualization of Sparse Immune Cell Populations During Inflammation.
Frontiers in immunology
Hofmann J,Gadjalova I,Mishra R,Ruland J,Keppler SJ
Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Immunocytochemistry
Wed Jun 16 00:00:00 EDT 2021
Meningeal lymphatic dysfunction exacerbates traumatic brain injury pathogenesis.
Nature communications
Bolte AC,Dutta AB,Hurt ME,Smirnov I,Kovacs MA,McKee CA,Ennerfelt HE,Shapiro D,Nguyen BH,Frost EL,Lammert CR,Kipnis J,Lukens JR
Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Immunohistochemistry
Thu Sep 10 00:00:00 EDT 2020
Local sympathetic innervations modulate the lung innate immune responses.
Science advances
Liu T,Yang L,Han X,Ding X,Li J,Yang J
14-0443 was used in Flow cytometry/Cell sorting to elucidate the critical function of local sympathetic innervations in negatively modulating the lung innate immune responses.
Fri May 01 00:00:00 EDT 2020
Structural and functional conservation of non-lumenized lymphatic endothelial cells in the mammalian leptomeninges.
Acta neuropathologica
Shibata-Germanos S,Goodman JR,Grieg A,Trivedi CA,Benson BC,Foti SC,Faro A,Castellan RFP,Correra RM,Barber M,Ruhrberg C,Weller RO,Lashley T,Iliff JJ,Hawkins TA,Rihel J
14-0443-82 was used in Immunohistochemistry to identify a structurally and functionally similar cell type in the mammalian leptomeninges that we name Leptomeningeal Lymphatic Endothelial Cells.
Sat Feb 01 00:00:00 EST 2020
CCBE1 promotes tumor lymphangiogenesis and is negatively regulated by TGFβ signaling in colorectal cancer.
Theranostics
Song J,Chen W,Cui X,Huang Z,Wen D,Yang Y,Yu W,Cui L,Liu CY
14-0443-82 was used in Immunohistochemistry to demonstrate the protumorigenic role of CCBE1 in promoting lymphangiogenesis and lymphatic metastasis in CRC.
Mon Feb 22 00:00:00 EST 2021
Disseminated Melanoma Cells Transdifferentiate into Endothelial Cells in Intravascular Niches at Metastatic Sites.
Cell reports
Li X,Karras P,Torres R,Rambow F,van den Oord J,Marine JC,Kos L
14-0443-82 was used in Flow Cytometry to observe tumour cell plasticity in melanoma cells.
Tue Jun 16 00:00:00 EDT 2020
Transcriptional mechanism of IRF8 and PU.1 governs microglial activation in neurodegenerative condition.
Protein & cell
Zhou N,Liu K,Sun Y,Cao Y,Yang J
14-0443 was used in Immunohistochemistry-immunofluorescence to elucidate the central transcriptional mechanism of microglial activation in response to neurodegenerative condition.
Fri Feb 01 00:00:00 EST 2019
Gut microbiota regulates lacteal integrity by inducing VEGF-C in intestinal villus macrophages.
EMBO reports
Suh SH,Choe K,Hong SP,Jeong SH,Mäkinen T,Kim KS,Alitalo K,Surh CD,Koh GY,Song JH
14-0443-82 was used in Immunohistochemistry to explore whether and how the gut microbiota regulates the integrity of the lacteal, a blunt-ended, long, tube-like lymphatic vessel located in the center of each intestinal villus that provides a unique route for drainage of absorbed lipids from the small intestine.
Mon Apr 01 00:00:00 EDT 2019
Neuroinflammation-induced lymphangiogenesis near the cribriform plate contributes to drainage of CNS-derived antigens and immune cells.
Nature communications
Hsu M,Rayasam A,Kijak JA,Choi YH,Harding JS,Marcus SA,Karpus WJ,Sandor M,Fabry Z
Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Immunohistochemistry
Wed Jan 16 00:00:00 EST 2019
Complex Non-sinus-associated Pachymeningeal Lymphatic Structures: Interrelationship With Blood Microvasculature.
Frontiers in physiology
Glinskii OV,Huxley VH,Xie L,Bunyak F,Palaniappan K,Glinsky VV
Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Immunofluorescence
Tue Sep 29 00:00:00 EDT 2020
Distinct origins and molecular mechanisms contribute to lymphatic formation during cardiac growth and regeneration.
eLife
Gancz D,Raftrey BC,Perlmoter G,Marín-Juez R,Semo J,Matsuoka RL,Karra R,Raviv H,Moshe N,Addadi Y,Golani O,Poss KD,Red-Horse K,Stainier DY,Yaniv K
14-0443-82 was used in Immunohistochemistry (PFA fixed) to provide novel insight into the mechanisms underlying lymphatic formation during development and regeneration, opening new avenues for interventions targeting specific lymphatic populations.
Fri Nov 08 00:00:00 EST 2019
A sphingosine-1-phosphate receptor 1 agonist inhibits tertiary lymphoid tissue reactivation and hypersensitivity in the lung.
Mucosal immunology
Huppé CA,Blais Lecours P,Lechasseur A,Gendron DR,Lemay AM,Bissonnette EY,Blanchet MR,Duchaine C,Morissette MC,Rosen H,Marsolais D
14-0443 was used in Immunohistochemistry-immunofluorescence to determine the effect of S1P1 on lymphocyte retention within tertiary lymphoid tissues in hypersensitivity pneumonitis.
Mon Jan 01 00:00:00 EST 2018
Lymphatic Endothelial Cells Produce M-CSF, Causing Massive Bone Loss in Mice.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
Wang W,Wang H,Zhou X,Li X,Sun W,Dellinger M,Boyce BF,Xing L
Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Immunohistochemistry
Mon May 01 00:00:00 EDT 2017
Lymphatic Endothelial Cells Produce M-CSF, Causing Massive Bone Loss in Mice.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
Wang W,Wang H,Zhou X,Li X,Sun W,Dellinger M,Boyce BF,Xing L
Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Immunohistochemistry
Mon May 01 00:00:00 EDT 2017
Long-term consequences of topical dexamethasone treatment during acute corneal HSV-1 infection on the immune system.
Journal of leukocyte biology
Chucair-Elliott AJ,Carr MM,Carr DJJ
Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Immunofluorescence
Mon May 01 00:00:00 EDT 2017
Defective lymphatic valve development and chylothorax in mice with a lymphatic-specific deletion of Connexin43.
Developmental biology
Munger SJ,Davis MJ,Simon AM
Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Immunohistochemistry
Sun Jan 15 00:00:00 EST 2017
Endogenous TNFα orchestrates the trafficking of neutrophils into and within lymphatic vessels during acute inflammation.
Scientific reports
Arokiasamy S,Zakian C,Dilliway J,Wang W,Nourshargh S,Voisin MB
14-0443 was used in Immunofluorescence to demonstrate a new role for TNFα as a key regulator of neutrophil trafficking into and within the lymphatic system.
Mon Mar 13 00:00:00 EDT 2017
Characterization of hepatic stellate cells, portal fibroblasts, and mesothelial cells in normal and fibrotic livers.
Journal of hepatology
Lua I,Li Y,Zagory JA,Wang KS,French SW,Sévigny J,Asahina K
Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Immunofluorescence
Sun May 01 00:00:00 EDT 2016
Thy1+IL-7+ lymphatic endothelial cells in iBALT provide a survival niche for memory T-helper cells in allergic airway inflammation.
Proceedings of the National Academy of Sciences of the United States of America
Shinoda K,Hirahara K,Iinuma T,Ichikawa T,Suzuki AS,Sugaya K,Tumes DJ,Yamamoto H,Hara T,Tani-Ichi S,Ikuta K,Okamoto Y,Nakayama T
14-0443 was used in Flow cytometry/Cell sorting to investigate how pathogenic Th cells are maintained at sites of local inflammation, showing that Thy1+IL-7+ lymphatic endothelial cells in iBALT provide a survival niche.
Tue May 17 00:00:00 EDT 2016
Lymphatic endothelial cells efferent to inflamed joints produce iNOS and inhibit lymphatic vessel contraction and drainage in TNF-induced arthritis in mice.
Arthritis research & therapy
Liang Q,Ju Y,Chen Y,Wang W,Li J,Zhang L,Xu H,Wood RW,Schwarz EM,Boyce BF,Wang Y,Xing L
Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Flow Cytometry
Sat Mar 12 00:00:00 EST 2016
Segregated Foxc2, NFATc1 and Connexin expression at normal developing venous valves, and Connexin-specific differences in the valve phenotypes of Cx37, Cx43, and Cx47 knockout mice.
Developmental biology
Munger SJ,Geng X,Srinivasan RS,Witte MH,Paul DL,Simon AM
Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Immunofluorescence
Fri Apr 15 00:00:00 EDT 2016
Diphtheria toxin-mediated ablation of lymphatic endothelial cells results in progressive lymphedema.
JCI insight
Gardenier JC,Hespe GE,Kataru RP,Savetsky IL,Torrisi JS,Nores GDG,Dayan JJ,Chang D,Zampell J,Martínez-Corral I,Ortega S,Mehrara BJ
Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Flow Cytometry
Thu Sep 22 00:00:00 EDT 2016
Effector lymphocyte-induced lymph node-like vasculature enables naive T-cell entry into tumours and enhanced anti-tumour immunity.
Nature communications
Peske JD,Thompson ED,Gemta L,Baylis RA,Fu YX,Engelhard VH
14044382 was used in immunohistochemistry to find that lymph node-like vasculature in melanoma and lung carcinoma murine models is both a consequence of and key contributor to anti-tumor immunity
Wed May 13 00:00:00 EDT 2015
Nociceptive sensory neurons drive interleukin-23-mediated psoriasiform skin inflammation.
Nature
Riol-Blanco L,Ordovas-Montanes J,Perro M,Naval E,Thiriot A,Alvarez D,Paust S,Wood JN,von Andrian UH
14-0443 was used in Immunohistochemistry-immunofluorescence to indicate that TRPV1(+)Nav1.8(+) nociceptors, by interacting with DDCs, regulate the IL-23/IL-17 pathway and control cutaneous immune responses.
Thu Jun 05 00:00:00 EDT 2014
VEGF-C and aortic cardiomyocytes guide coronary artery stem development.
The Journal of clinical investigation
Chen HI,Poduri A,Numi H,Kivela R,Saharinen P,McKay AS,Raftrey B,Churko J,Tian X,Zhou B,Wu JC,Alitalo K,Red-Horse K
14-0443 was used in Immunohistochemistry to demonstrate that VEGF-C-deficient hearts have severely hypoplastic peritruncal vessels, resulting in delayed and abnormally positioned coronary artery stems.
Sat Nov 01 00:00:00 EDT 2014
Schlemm's canal is a unique vessel with a combination of blood vascular and lymphatic phenotypes that forms by a novel developmental process.
PLoS biology
Kizhatil K,Ryan M,Marchant JK,Henrich S,John SW
14-0443 was used in Immunofluorescence to study how SC development resembles a blend of vascular developmental programs.
Tue Jul 01 00:00:00 EDT 2014
Identification of targets of Prox1 during in vitro vascular differentiation from embryonic stem cells: functional roles of HoxD8 in lymphangiogenesis.
Journal of cell science
Harada K,Yamazaki T,Iwata C,Yoshimatsu Y,Sase H,Mishima K,Morishita Y,Hirashima M,Oike Y,Suda T,Miura N,Watabe T,Miyazono K
Sun Nov 01 00:00:00 EDT 2009