Invitrogen

LYVE1 Monoclonal Antibody (ALY7), eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

31 Published Figures

50 References

View all (41) LYVE1 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

LYVE1 Antibody in Immunohistochemistry (Frozen) (IHC (F))

FIGURE: 1 / 33

LYVE1 Antibody (14-0443-82) in IHC (F)

Immunohistochemistry of frozen mouse intestine stained with 2.5 µg/mL Rat IgG1 K Isotype Control Purified (left) or 2.5 µg/mL Anti-Mouse Lyve-1 Purified (right) followed by 5 µg/mL Anti-Rat IgG1 eFluor® 615. Nuclei are stained with DAPI.

LYVE1 Antibody in Immunohistochemistry (IHC)

LYVE1 Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)

LYVE1 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

Product Image Gallery

LYVE1 Antibody — Figure 7. Long-term effect of topical dexamethasone treatment during the first 8 d of HSV-1 infection on corneal innervation and vasculature. Mouse corneas were infected with 10 3 PFU HSV-1 or left U) as controls. Starting at 2 h p.i., mice were topically treated with DEX or VEH onto their corneas for 8 d p.i. before tissue collection at 30 d p.i. (A) Representative confocal images show corneal nerves (green: beta-III tubulin staining) and blood and lymphatic vessels (red: CD31 staining; blue: LYVE-1 staining, top discontinuous white lines depict the limbal margins) at 30 d p.i. (top: merge of beta-III tubulin/CD3/LYVE-1 costaining, bottom: grayscale display of beta-III tubulin staining only). White arrows depict intact fine bundles of subbasal nerves in the UI group that are preserved in the infected group treated with DEX. (B) Analysis of corneal innervation (left) and vascularization (center and right) expressed as the percentage threshold area positive for beta-III tubulin signal per field of view means +- sem , percentage threshold area positive for CD31 signal per field of view means +- sem , and percentage threshold area positive for LYVE-1 signal per field of view means +- sem, respectively ( n = 6 for UI and n = 16-17 for infected groups for beta-III tubulin, n = 11 for UI and n = 16-23 for uninfected groups for CD31, and n = 9 for UI and n = 11-16 per infected groups for LYVE-1 from 3-4 independent experiments). (C) Bars show Cochet-Bonnet {TM}

Product Details

14-0443-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 27 publications 27 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 7 publications 7 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Immunocytochemistry (ICC/IF)

Assay-Dependent

View 2 publications 2 publications

Flow Cytometry (Flow)

0.125 µg/Test

View 14 publications 14 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

ALY7

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1633414

Product Specific Information

Description: The monoclonal antibody ALY7 recognizes mouse LYVE-1, a transmembrane glycoprotein with similarity to CD44. The extracellular domain contains a conserved hyaluronan binding domain also found in CD44. Expression is found on lymphatic and liver endothelial cells and some populations of macrophages. The lymphatic system is responsible for transporting proteins and cells (especially dendritic cells) to tissues throughout the body, thereby acting as immune surveyors. LYVE-1 is one characteristic protein, along with podoplanin, PROX-1, Tie-2 and VEGFR-3, that is expressed on lymphatic endothelial cells (LECS). The ligand for LYVE-1 is hyaluronan, a large mucopolysaccharide. Although LYVE-1 can bind hyaluronan in vitro, the site for ligand binding in vivo is masked by sialyated O-linked glycan chains. It is postulated that binding to ligand requires modification/unmasking to expose the binding site. The development and remodeling of the endothelium after injury is an area of extensive study. When transplanted, hematopoietic stem cells (HSCs) can give rise to LECs that integrate into the endothelium in normal and metastatic tissue.

Applications Reported: This ALY7 antibody has been reported for use in flow cytometric analysis and immunohistologic staining of frozen tissue sections.

Applications Tested: This ALY7 antibody has been tested by flow cytometric analysis of transfected cell line or immunofluorescent microscopy of cryosections of mouse intestine. This can be used at less than or equal to 0.125 µg per million cells in a 100 µL total staining volume for flow cytometry and 2.5 µg/mL for immunofluorescent micoscopy. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

LYVE1 has been identified as a major receptor for HA (extracellular matrix glycosaminoglycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE1 predicts a 322-residue type I integral membrane polypeptide 41% similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module, the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE1 molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves. LYVE1 is a type I integral membrane glycoprotein. LYVE-1 is expressed primarily on lymphatic vessel endothelium and is likely to be involved in regulating the traffic of leucocytes and tumor cells to lymph nodes. The lymphatic vasculature forms a second circulatory system that drains extracellular fluid from the tissues and provides an exclusive environment in which immune cells can encounter and respond to foreign antigen. A number of molecules have been identified as markers for lymphatic endothelium which include LYVE1, PALE, VEGFR3, and podoplanin. Diseases associated with LYVE1 dysfunction includes Complete Androgen Insensitivity Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CD206⁺ macrophages transventricularly infiltrate the early embryonic cerebral wall to differentiate into microglia.

Cell reports

Hattori Y,Kato D,Murayama F,Koike S,Asai H,Yamasaki A,Naito Y,Kawaguchi A,Konishi H,Prinz M,Masuda T,Wake H,Miyata T

14-0443-82 was used in Immunocytochemistry-immunofluorescence to demonstrate that the "roof plate?ventricle?pallium" route is an essential path for microglial colonization into the embryonic mouse brain.

Tue Feb 28 00:00:00 EST 2023

CD206⁺ macrophages transventricularly infiltrate the early embryonic cerebral wall to differentiate into microglia.

Cell reports

Hattori Y,Kato D,Murayama F,Koike S,Asai H,Yamasaki A,Naito Y,Kawaguchi A,Konishi H,Prinz M,Masuda T,Wake H,Miyata T

Tue Feb 28 00:00:00 EST 2023

Bacterial meningitis in the early postnatal mouse studied at single-cell resolution.

eLife

Wang J,Rattner A,Nathans J

14-0443-82 was used in Immunohistochemistry to imply that EC responses to infection are largely driven by the intrinsic EC response to LPS.

Thu Jun 15 00:00:00 EDT 2023

Activating SIRT3 in peritoneal mesothelial cells alleviates postsurgical peritoneal adhesion formation by decreasing oxidative stress and inhibiting the NLRP3 inflammasome.

Experimental & molecular medicine

Shen T,Wu Y,Wang X,Wang Z,Li E,Zhou C,Yue C,Jiang Z,Wei G,Lian J,Xu Q,Li X

14-0443-82 was used in Flow Cytometry to show that the rescue of SIRT3 by HKL may be a new therapeutic strategy to alleviate and block postsurgical PA formation.

Thu Sep 01 00:00:00 EDT 2022

Immuno-localization of definitive hematopoietic stem cells in the vascular niche of mouse fetal liver.

STAR protocols

Biswas A,Singh SK,Kartha GM,Khurana S

Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Immunohistochemistry

Fri Dec 16 00:00:00 EST 2022

Deciphering the heterogeneity of the Lyve1⁺ perivascular macrophages in the mouse brain.

Nature communications

Siret C,van Lessen M,Bavais J,Jeong HW,Reddy Samawar SK,Kapupara K,Wang S,Simic M,de Fabritus L,Tchoghandjian A,Fallet M,Huang H,Sarrazin S,Sieweke MH,Stumm R,Sorokin L,Adams RH,Schulte-Merker S,Kiefer F,van de Pavert SA

Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Flow Cytometry

Wed Nov 30 00:00:00 EST 2022

Absence of microglia promotes diverse pathologies and early lethality in Alzheimer's disease mice.

Cell reports

Kiani Shabestari S,Morabito S,Danhash EP,McQuade A,Sanchez JR,Miyoshi E,Chadarevian JP,Claes C,Coburn MA,Hasselmann J,Hidalgo J,Tran KN,Martini AC,Chang Rothermich W,Pascual J,Head E,Hume DA,Pridans C,Davtyan H,Swarup V,Blurton-Jones M

14-0443-82 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen) to indicate the protective functions of microglia in reducing CAA, blood-brain barrier dysfunction, and brain calcification.

Tue Jun 14 00:00:00 EDT 2022

The E3 ubiquitin ligase WWP2 regulates pro-fibrogenic monocyte infiltration and activity in heart fibrosis.

Nature communications

Chen H,Chew G,Devapragash N,Loh JZ,Huang KY,Guo J,Liu S,Tan ELS,Chen S,Tee NGZ,Mia MM,Singh MK,Zhang A,Behmoaras J,Petretto E

14-0443-82 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen) to identify a pro-fibrogenic macrophage subtype in non-ischemic cardiomyopathy, and demonstrate that WWP2 is a key regulator of IRF7-mediated Ccl5/Ly6chigh monocyte axis in heart fibrosis.

Wed Nov 30 00:00:00 EST 2022

Integrins mediate placental extracellular vesicle trafficking to lung and liver in vivo.

Scientific reports

Nguyen SL,Ahn SH,Greenberg JW,Collaer BW,Agnew DW,Arora R,Petroff MG

Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Immunohistochemistry

Thu Feb 18 00:00:00 EST 2021

Nasal administration of mitochondria reverses chemotherapy-induced cognitive deficits.

Theranostics

Alexander JF,Seua AV,Arroyo LD,Ray PR,Wangzhou A,Heiβ-Lückemann L,Schedlowski M,Price TJ,Kavelaars A,Heijnen CJ

14-0443-82 was used in Sample Preparation to whether nasal administration of mitochondria isolated from human mesenchymal stem cells to mice, can reverse cisplatin-induced cognitive deficits.

Tue Jul 27 00:00:00 EDT 2021

Nasal administration of mitochondria reverses chemotherapy-induced cognitive deficits.

Theranostics

Alexander JF,Seua AV,Arroyo LD,Ray PR,Wangzhou A,Heiβ-Lückemann L,Schedlowski M,Price TJ,Kavelaars A,Heijnen CJ

14-0443-82 was used in Sample Preparation to whether nasal administration of mitochondria isolated from human mesenchymal stem cells to mice, can reverse cisplatin-induced cognitive deficits.

Tue Jul 27 00:00:00 EDT 2021

Nasal administration of mitochondria reverses chemotherapy-induced cognitive deficits.

Theranostics

Alexander JF,Seua AV,Arroyo LD,Ray PR,Wangzhou A,Heiβ-Lückemann L,Schedlowski M,Price TJ,Kavelaars A,Heijnen CJ

14-0443-82 was used in Sample Preparation to whether nasal administration of mitochondria isolated from human mesenchymal stem cells to mice, can reverse cisplatin-induced cognitive deficits.

Tue Jul 27 00:00:00 EDT 2021

Interleukin-11-expressing fibroblasts have a unique gene signature correlated with poor prognosis of colorectal cancer.

Nature communications

Nishina T,Deguchi Y,Ohshima D,Takeda W,Ohtsuka M,Shichino S,Ueha S,Yamazaki S,Kawauchi M,Nakamura E,Nishiyama C,Kojima Y,Adachi-Akahane S,Hasegawa M,Nakayama M,Oshima M,Yagita H,Shibuya K,Mikami T,Inohara N,Matsushima K,Tada N,Nakano H

Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Flow Cytometry

Fri Apr 16 00:00:00 EDT 2021

Extracellular ATP Limits Homeostatic T Cell Migration Within Lymph Nodes.

Frontiers in immunology

Kobayashi D,Sugiura Y,Umemoto E,Takeda A,Ueta H,Hayasaka H,Matsuzaki S,Katakai T,Suematsu M,Hamachi I,Yegutkin GG,Salmi M,Jalkanen S,Miyasaka M

Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Immunohistochemistry

Mon Feb 14 00:00:00 EST 2022

Targeting local lymphatics to ameliorate heterotopic ossification via FGFR3-BMPR1a pathway.

Nature communications

Zhang D,Huang J,Sun X,Chen H,Huang S,Yang J,Du X,Tan Q,Luo F,Zhang R,Zhou S,Jiang W,Ni Z,Wang Z,Jin M,Xu M,Li F,Chen L,Liu M,Su N,Luo X,Yin L,Zhu Y,Feng JQ,Chen D,Qi H,Chen L,Xie Y

Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Immunocytochemistry

Mon Jul 19 00:00:00 EDT 2021

Lymphocyte access to lymphoma is impaired by high endothelial venule regression.

Cell reports

Menzel L,Zschummel M,Crowley T,Franke V,Grau M,Ulbricht C,Hauser A,Siffrin V,Bajénoff M,Acton SE,Akalin A,Lenz G,Willimsky G,Höpken UE,Rehm A

14-0443-82 was used in Immunohistochemistry to show that lymphoma causes severe high endothelial venule regression in mouse models that phenocopies aggressive human B cell lymphomas.

Tue Oct 26 00:00:00 EDT 2021

SPNS2 enables T cell egress from lymph nodes during an immune response.

Cell reports

Okuniewska M,Fang V,Baeyens A,Raghavan V,Lee JY,Littman DR,Schwab SR

14-0443-82 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen) to demonstrate that SPNS2 supplies the S1P that guides T cells out of LNs with an ongoing immune response.

Tue Jul 13 00:00:00 EDT 2021

Meningeal lymphatic vessels regulate brain tumor drainage and immunity.

Cell research

Hu X,Deng Q,Ma L,Li Q,Chen Y,Liao Y,Zhou F,Zhang C,Shao L,Feng J,He T,Ning W,Kong Y,Huo Y,He A,Liu B,Zhang J,Adams R,He Y,Tang F,Bian X,Luo J

Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Flow Cytometry

Sun Mar 01 00:00:00 EST 2020

Meningeal lymphatic vessels regulate brain tumor drainage and immunity.

Cell research

Hu X,Deng Q,Ma L,Li Q,Chen Y,Liao Y,Zhou F,Zhang C,Shao L,Feng J,He T,Ning W,Kong Y,Huo Y,He A,Liu B,Zhang J,Adams R,He Y,Tang F,Bian X,Luo J

Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Flow Cytometry

Sun Mar 01 00:00:00 EST 2020

An ocular glymphatic clearance system removes β-amyloid from the rodent eye.

Science translational medicine

Wang X,Lou N,Eberhardt A,Yang Y,Kusk P,Xu Q,Förstera B,Peng S,Shi M,Ladrón-de-Guevara A,Delle C,Sigurdsson B,Xavier ALR,Ertürk A,Libby RT,Chen L,Thrane AS,Nedergaard M

14-0443 was used in Immunocytochemistry to identify an ocular glymphatic clearance route for fluid and wastes via the proximal optic nerve in rodents.

Wed Mar 25 00:00:00 EDT 2020

Tissue-resident macrophages in omentum promote metastatic spread of ovarian cancer.

The Journal of experimental medicine

Etzerodt A,Moulin M,Doktor TK,Delfini M,Mossadegh-Keller N,Bajenoff M,Sieweke MH,Moestrup SK,Auphan-Anezin N,Lawrence T

Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Flow Cytometry

Mon Apr 06 00:00:00 EDT 2020

FK506 induces lung lymphatic endothelial cell senescence and downregulates LYVE-1 expression, with associated decreased hyaluronan uptake.

Molecular medicine (Cambridge, Mass.)

Shrestha S,Cho W,Stump B,Imani J,Lamattina AM,Louis PH,Pazzanese J,Rosas IO,Visner G,Perrella MA,El-Chemaly S

Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Flow Cytometry

Fri Jul 31 00:00:00 EDT 2020

Anatomic evidence shows that lymphatic drainage exists in the pituitary to loop the cerebral lymphatic circulation.

Medical hypotheses

Elham E,Wumaier R,Wang C,Luo X,Chen T,Zhong N

Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Immunohistochemistry

Thu Oct 01 00:00:00 EDT 2020

Meningeal lymphatics clear erythrocytes that arise from subarachnoid hemorrhage.

Nature communications

Chen J,Wang L,Xu H,Xing L,Zhuang Z,Zheng Y,Li X,Wang C,Chen S,Guo Z,Liang Q,Wang Y

Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Immunohistochemistry

Mon Jun 22 00:00:00 EDT 2020

Efficient Tissue Clearing and Multi-Organ Volumetric Imaging Enable Quantitative Visualization of Sparse Immune Cell Populations During Inflammation.

Frontiers in immunology

Hofmann J,Gadjalova I,Mishra R,Ruland J,Keppler SJ

Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Immunocytochemistry

Wed Jun 16 00:00:00 EDT 2021

Meningeal lymphatic dysfunction exacerbates traumatic brain injury pathogenesis.

Nature communications

Bolte AC,Dutta AB,Hurt ME,Smirnov I,Kovacs MA,McKee CA,Ennerfelt HE,Shapiro D,Nguyen BH,Frost EL,Lammert CR,Kipnis J,Lukens JR

Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Immunohistochemistry

Thu Sep 10 00:00:00 EDT 2020

Local sympathetic innervations modulate the lung innate immune responses.

Science advances

Liu T,Yang L,Han X,Ding X,Li J,Yang J

14-0443 was used in Flow cytometry/Cell sorting to elucidate the critical function of local sympathetic innervations in negatively modulating the lung innate immune responses.

Fri May 01 00:00:00 EDT 2020

Structural and functional conservation of non-lumenized lymphatic endothelial cells in the mammalian leptomeninges.

Acta neuropathologica

Shibata-Germanos S,Goodman JR,Grieg A,Trivedi CA,Benson BC,Foti SC,Faro A,Castellan RFP,Correra RM,Barber M,Ruhrberg C,Weller RO,Lashley T,Iliff JJ,Hawkins TA,Rihel J

14-0443-82 was used in Immunohistochemistry to identify a structurally and functionally similar cell type in the mammalian leptomeninges that we name Leptomeningeal Lymphatic Endothelial Cells.

Sat Feb 01 00:00:00 EST 2020

CCBE1 promotes tumor lymphangiogenesis and is negatively regulated by TGFβ signaling in colorectal cancer.

Theranostics

Song J,Chen W,Cui X,Huang Z,Wen D,Yang Y,Yu W,Cui L,Liu CY

14-0443-82 was used in Immunohistochemistry to demonstrate the protumorigenic role of CCBE1 in promoting lymphangiogenesis and lymphatic metastasis in CRC.

Mon Feb 22 00:00:00 EST 2021

Disseminated Melanoma Cells Transdifferentiate into Endothelial Cells in Intravascular Niches at Metastatic Sites.

Cell reports

Li X,Karras P,Torres R,Rambow F,van den Oord J,Marine JC,Kos L

14-0443-82 was used in Flow Cytometry to observe tumour cell plasticity in melanoma cells.

Tue Jun 16 00:00:00 EDT 2020

Transcriptional mechanism of IRF8 and PU.1 governs microglial activation in neurodegenerative condition.

Protein & cell

Zhou N,Liu K,Sun Y,Cao Y,Yang J

14-0443 was used in Immunohistochemistry-immunofluorescence to elucidate the central transcriptional mechanism of microglial activation in response to neurodegenerative condition.

Fri Feb 01 00:00:00 EST 2019

Gut microbiota regulates lacteal integrity by inducing VEGF-C in intestinal villus macrophages.

EMBO reports

Suh SH,Choe K,Hong SP,Jeong SH,Mäkinen T,Kim KS,Alitalo K,Surh CD,Koh GY,Song JH

14-0443-82 was used in Immunohistochemistry to explore whether and how the gut microbiota regulates the integrity of the lacteal, a blunt-ended, long, tube-like lymphatic vessel located in the center of each intestinal villus that provides a unique route for drainage of absorbed lipids from the small intestine.

Mon Apr 01 00:00:00 EDT 2019

Neuroinflammation-induced lymphangiogenesis near the cribriform plate contributes to drainage of CNS-derived antigens and immune cells.

Nature communications

Hsu M,Rayasam A,Kijak JA,Choi YH,Harding JS,Marcus SA,Karpus WJ,Sandor M,Fabry Z

Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Immunohistochemistry

Wed Jan 16 00:00:00 EST 2019

Complex Non-sinus-associated Pachymeningeal Lymphatic Structures: Interrelationship With Blood Microvasculature.

Frontiers in physiology

Glinskii OV,Huxley VH,Xie L,Bunyak F,Palaniappan K,Glinsky VV

Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Immunofluorescence

Tue Sep 29 00:00:00 EDT 2020

Distinct origins and molecular mechanisms contribute to lymphatic formation during cardiac growth and regeneration.

eLife

Gancz D,Raftrey BC,Perlmoter G,Marín-Juez R,Semo J,Matsuoka RL,Karra R,Raviv H,Moshe N,Addadi Y,Golani O,Poss KD,Red-Horse K,Stainier DY,Yaniv K

14-0443-82 was used in Immunohistochemistry (PFA fixed) to provide novel insight into the mechanisms underlying lymphatic formation during development and regeneration, opening new avenues for interventions targeting specific lymphatic populations.

Fri Nov 08 00:00:00 EST 2019

A sphingosine-1-phosphate receptor 1 agonist inhibits tertiary lymphoid tissue reactivation and hypersensitivity in the lung.

Mucosal immunology

Huppé CA,Blais Lecours P,Lechasseur A,Gendron DR,Lemay AM,Bissonnette EY,Blanchet MR,Duchaine C,Morissette MC,Rosen H,Marsolais D

Mon Jan 01 00:00:00 EST 2018

Lymphatic Endothelial Cells Produce M-CSF, Causing Massive Bone Loss in Mice.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Wang W,Wang H,Zhou X,Li X,Sun W,Dellinger M,Boyce BF,Xing L

Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Flow Cytometry

Mon May 01 00:00:00 EDT 2017

Lymphatic Endothelial Cells Produce M-CSF, Causing Massive Bone Loss in Mice.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Wang W,Wang H,Zhou X,Li X,Sun W,Dellinger M,Boyce BF,Xing L

Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Flow Cytometry

Mon May 01 00:00:00 EDT 2017

Long-term consequences of topical dexamethasone treatment during acute corneal HSV-1 infection on the immune system.

Journal of leukocyte biology

Chucair-Elliott AJ,Carr MM,Carr DJJ

Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Immunofluorescence

Mon May 01 00:00:00 EDT 2017

Defective lymphatic valve development and chylothorax in mice with a lymphatic-specific deletion of Connexin43.

Developmental biology

Munger SJ,Davis MJ,Simon AM

Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Immunohistochemistry

Sun Jan 15 00:00:00 EST 2017

Endogenous TNFα orchestrates the trafficking of neutrophils into and within lymphatic vessels during acute inflammation.

Scientific reports

Arokiasamy S,Zakian C,Dilliway J,Wang W,Nourshargh S,Voisin MB

14-0443 was used in Immunofluorescence to demonstrate a new role for TNFα as a key regulator of neutrophil trafficking into and within the lymphatic system.

Mon Mar 13 00:00:00 EDT 2017

Characterization of hepatic stellate cells, portal fibroblasts, and mesothelial cells in normal and fibrotic livers.

Journal of hepatology

Lua I,Li Y,Zagory JA,Wang KS,French SW,Sévigny J,Asahina K

Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Immunohistochemistry

Sun May 01 00:00:00 EDT 2016

Thy1+IL-7+ lymphatic endothelial cells in iBALT provide a survival niche for memory T-helper cells in allergic airway inflammation.

Proceedings of the National Academy of Sciences of the United States of America

Shinoda K,Hirahara K,Iinuma T,Ichikawa T,Suzuki AS,Sugaya K,Tumes DJ,Yamamoto H,Hara T,Tani-Ichi S,Ikuta K,Okamoto Y,Nakayama T

14-0443 was used in Flow cytometry/Cell sorting to investigate how pathogenic Th cells are maintained at sites of local inflammation, showing that Thy1+IL-7+ lymphatic endothelial cells in iBALT provide a survival niche.

Tue May 17 00:00:00 EDT 2016

Lymphatic endothelial cells efferent to inflamed joints produce iNOS and inhibit lymphatic vessel contraction and drainage in TNF-induced arthritis in mice.

Arthritis research & therapy

Liang Q,Ju Y,Chen Y,Wang W,Li J,Zhang L,Xu H,Wood RW,Schwarz EM,Boyce BF,Wang Y,Xing L

Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Flow Cytometry

Sat Mar 12 00:00:00 EST 2016

Segregated Foxc2, NFATc1 and Connexin expression at normal developing venous valves, and Connexin-specific differences in the valve phenotypes of Cx37, Cx43, and Cx47 knockout mice.

Developmental biology

Munger SJ,Geng X,Srinivasan RS,Witte MH,Paul DL,Simon AM

Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Immunofluorescence

Fri Apr 15 00:00:00 EDT 2016

Diphtheria toxin-mediated ablation of lymphatic endothelial cells results in progressive lymphedema.

JCI insight

Gardenier JC,Hespe GE,Kataru RP,Savetsky IL,Torrisi JS,Nores GDG,Dayan JJ,Chang D,Zampell J,Martínez-Corral I,Ortega S,Mehrara BJ

Published figure using LYVE1 monoclonal antibody (Product # 14-0443-82) in Flow Cytometry

Thu Sep 22 00:00:00 EDT 2016

Effector lymphocyte-induced lymph node-like vasculature enables naive T-cell entry into tumours and enhanced anti-tumour immunity.

Nature communications

Peske JD,Thompson ED,Gemta L,Baylis RA,Fu YX,Engelhard VH

14044382 was used in immunohistochemistry to find that lymph node-like vasculature in melanoma and lung carcinoma murine models is both a consequence of and key contributor to anti-tumor immunity

Wed May 13 00:00:00 EDT 2015

Nociceptive sensory neurons drive interleukin-23-mediated psoriasiform skin inflammation.

Nature

Riol-Blanco L,Ordovas-Montanes J,Perro M,Naval E,Thiriot A,Alvarez D,Paust S,Wood JN,von Andrian UH

14-0443 was used in Immunohistochemistry-immunofluorescence to indicate that TRPV1(+)Nav1.8(+) nociceptors, by interacting with DDCs, regulate the IL-23/IL-17 pathway and control cutaneous immune responses.

Thu Jun 05 00:00:00 EDT 2014

VEGF-C and aortic cardiomyocytes guide coronary artery stem development.

The Journal of clinical investigation

Chen HI,Poduri A,Numi H,Kivela R,Saharinen P,McKay AS,Raftrey B,Churko J,Tian X,Zhou B,Wu JC,Alitalo K,Red-Horse K

14-0443 was used in Immunohistochemistry to demonstrate that VEGF-C-deficient hearts have severely hypoplastic peritruncal vessels, resulting in delayed and abnormally positioned coronary artery stems.

Sat Nov 01 00:00:00 EDT 2014

Schlemm's canal is a unique vessel with a combination of blood vascular and lymphatic phenotypes that forms by a novel developmental process.

PLoS biology

Kizhatil K,Ryan M,Marchant JK,Henrich S,John SW

14-0443 was used in Immunofluorescence to study how SC development resembles a blend of vascular developmental programs.

Tue Jul 01 00:00:00 EDT 2014

Identification of targets of Prox1 during in vitro vascular differentiation from embryonic stem cells: functional roles of HoxD8 in lymphangiogenesis.

Journal of cell science

Harada K,Yamazaki T,Iwata C,Yoshimatsu Y,Sase H,Mishima K,Morishita Y,Hirashima M,Oike Y,Suda T,Miura N,Watabe T,Miyazono K

Sun Nov 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: cell surface retention sequence binding protein-1; Cell surface retention sequence-binding protein 1; CRSBP-1; extra cellular link domain-containing 1; Extracellular link domain-containing protein 1; lymphatic vessel endothelial HA receptor-1; lymphatic vessel endothelial HA recptor-1; Lymphatic vessel endothelial hyaluronic acid receptor 1; LYVE-1; sLyve 1; sLyve1; soluble LYVE 1; soluble LYVE1

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Gene Aliases: 1200012G08Rik; Crsbp-1; Crsbp1; Lyve-1; Lyve1; Xlkd1

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UniProt ID: (Mouse) Q8BHC0

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Entrez Gene ID: (Mouse) 114332

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Function(s)

transmembrane signaling receptor activity hyaluronic acid binding transmembrane signal receptor

Process(es)

glycosaminoglycan catabolic process transport cell adhesion signal transduction

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LYVE1 Monoclonal Antibody (ALY7), eBioscience™

LYVE1 Antibody (14-0443-82) in IHC (F)

Product Details

14-0443-82

Immunohistochemistry (IHC)

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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