Invitrogen

LYVE1 Monoclonal Antibody (ALY7), Alexa Fluor™ 488, eBioscience™

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Antibody Testing Data (1)
Published Figures (33)

LYVE1 Antibody in Immunohistochemistry (Frozen) (IHC (F))

FIGURE: 1 / 34

LYVE1 Antibody (53-0443-82) in IHC (F)

Immunohistochemistry of cryosections of mouse intestine at 1 µg/mL of Anti-Mouse Lyve-1 Alexa Fluor® 488 (right). Phase image of same field (left).

LYVE1 Antibody in Immunohistochemistry (IHC)

LYVE1 Antibody in Immunocytochemistry (ICC/IF)

LYVE1 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

LYVE1 Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)

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Product Details

53-0443-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 23 publications 23 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

Assay-Dependent

View 8 publications 8 publications

Flow Cytometry (Flow)

Assay-Dependent

View 14 publications 14 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

ALY7

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1633415

Product Specific Information

Description: The monoclonal antibody ALY7 recognizes mouse LYVE-1, a transmembrane glycoprotein with similarity to CD44. The extracellular domain contains a conserved hyaluronan binding domain also found in CD44. Expression is found on lymphatic and liver endothelial cells and some populations of macrophages. The lymphatic system is responsible for transporting proteins and cells (especially dendritic cells) to tissues throughout the body, thereby acting as immune surveyors. LYVE-1 is one characteristic protein, along with podoplanin, PROX-1, Tie-2 and VEGFR-3, that is expressed on lymphatic endothelial cells (LECS). The ligand for LYVE-1 is hyaluronan, a large mucopolysaccharide. Although LYVE-1 can bind hyaluronan in vitro, the site for ligand binding in vivo is masked by sialyated O-linked glycan chains. It is postulated that binding to ligand requires modification/unmasking to expose the binding site. The development and remodeling of the endothelium after injury is an area of extensive study. When transplanted, hematopoietic stem cells (HSCs) can give rise to LECs that integrate into the endothelium in normal and metastatic tissue.

Applications Reported: This ALY7 antibody has been reported for use in flow cytometric analysis and immunohistologic staining of frozen tissue sections.

Applications Tested: This ALY7 antibody has been tested by immunofluorescence microscopy on mouse intestine. This can be used at less than or equal to 2 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

LYVE1 has been identified as a major receptor for HA (extracellular matrix glycosaminoglycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE1 predicts a 322-residue type I integral membrane polypeptide 41% similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module, the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE1 molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves. LYVE1 is a type I integral membrane glycoprotein. LYVE-1 is expressed primarily on lymphatic vessel endothelium and is likely to be involved in regulating the traffic of leucocytes and tumor cells to lymph nodes. The lymphatic vasculature forms a second circulatory system that drains extracellular fluid from the tissues and provides an exclusive environment in which immune cells can encounter and respond to foreign antigen. A number of molecules have been identified as markers for lymphatic endothelium which include LYVE1, PALE, VEGFR3, and podoplanin. Diseases associated with LYVE1 dysfunction includes Complete Androgen Insensitivity Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Low protease activity in B cell follicles promotes retention of intact antigens after immunization.

Science (New York, N.Y.)

Aung A,Cui A,Maiorino L,Amini AP,Gregory JR,Bukenya M,Zhang Y,Lee H,Cottrell CA,Morgan DM,Silva M,Suh H,Kirkpatrick JD,Amlashi P,Remba T,Froehle LM,Xiao S,Abraham W,Adams J,Love JC,Huyett P,Kwon DS,Hacohen N,Schief WR,Bhatia SN,Irvine DJ

53-0443-82 was used in Immunohistochemistry-immunofluorescence to characterize protease activity in lymph nodes and found that antigens were rapidly degraded in the subcapsular sinus, paracortex, and interfollicular regions, whereas low protease activity and antigen degradation rates were detected in the vicinity of follicular dendritic cells (FDCs).

Fri Jan 27 00:00:00 EST 2023

Primary tumor-derived systemic nANGPTL4 inhibits metastasis.

The Journal of experimental medicine

Hübers C,Abdul Pari AA,Grieshober D,Petkov M,Schmidt A,Messmer T,Heyer CM,Schölch S,Kapel SS,Gengenbacher N,Singhal M,Schieb B,Fricke C,Will R,Remans K,Utikal JS,Reissfelder C,Schlesner M,Hodivala-Dilke KM,Kersten S,Goerdt S,Augustin HG,Felcht M

53-0443-82 was used in Flow cytometry/Cell sorting to highlight the spatial context of the proteolytic cleavage-dependent pro- and antitumorigenic functions of ANGPTL4 and identifies and validates nANGPTL4 as a novel biomarker of tumor progression and antimetastatic therapeutic agent.

Mon Jan 02 00:00:00 EST 2023

Immuno-localization of definitive hematopoietic stem cells in the vascular niche of mouse fetal liver.

STAR protocols

Biswas A,Singh SK,Kartha GM,Khurana S

Published figure using LYVE1 monoclonal antibody (Product # 53-0443-82) in Immunohistochemistry

Fri Dec 16 00:00:00 EST 2022

Deciphering the heterogeneity of the Lyve1⁺ perivascular macrophages in the mouse brain.

Nature communications

Siret C,van Lessen M,Bavais J,Jeong HW,Reddy Samawar SK,Kapupara K,Wang S,Simic M,de Fabritus L,Tchoghandjian A,Fallet M,Huang H,Sarrazin S,Sieweke MH,Stumm R,Sorokin L,Adams RH,Schulte-Merker S,Kiefer F,van de Pavert SA

Published figure using LYVE1 monoclonal antibody (Product # 53-0443-82) in Flow Cytometry

Wed Nov 30 00:00:00 EST 2022

A single-cell atlas reveals the heterogeneity of meningeal immunity in a mouse model of Methyl CpG binding protein 2 deficiency.

Frontiers in immunology

Li H,Hu M,Huang Z,Wang Y,Xu Y,Deng J,Zhu M,Feng W,Xu X

53-0443-82 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen) to provide novel insights into meningeal immunity, which is a less studied aspect of neuroimmune interactions in Mecp2-mutated diseases, and offer an essential resource for comparative analyses and data exploration to better understand the functional role of meningeal immunity in RTT.

Tue Jan 31 00:00:00 EST 2023

A Binary Cre Transgenic Approach Dissects Microglia and CNS Border-Associated Macrophages.

Immunity

Kim JS,Kolesnikov M,Peled-Hajaj S,Scheyltjens I,Xia Y,Trzebanski S,Haimon Z,Shemer A,Lubart A,Van Hove H,Chappell-Maor L,Boura-Halfon S,Movahedi K,Blinder P,Jung S

53-0443-82 was used in Flow Cytometry to establish the value of binary transgenesis to dissect tissue macrophage compartments and their functions.

Tue Jan 12 00:00:00 EST 2021

Lymph node formation and B cell homeostasis require IKK-α in distinct endothelial cell-derived compartments.

Proceedings of the National Academy of Sciences of the United States of America

McCorkell KA,Jayachandran N,Cully MD,Freund-Brown J,Weinkopff T,Monslow J,Hu Y,Puré E,Freedman BD,Alvarez JI,Cancro MP,May MJ

53-0443-82 was used in Immunohistochemistry-immunofluorescence to reveal that IKK-α in distinct EC-derived compartments is uniquely required to promote B cell homeostasis and LN development, and establish that LEC-intrinsic IKK-α is absolutely essential for LN formation.

Tue Nov 30 00:00:00 EST 2021

Integrins mediate placental extracellular vesicle trafficking to lung and liver in vivo.

Scientific reports

Nguyen SL,Ahn SH,Greenberg JW,Collaer BW,Agnew DW,Arora R,Petroff MG

Published figure using LYVE1 monoclonal antibody (Product # 53-0443-82) in Immunohistochemistry

Thu Feb 18 00:00:00 EST 2021

Nasal administration of mitochondria reverses chemotherapy-induced cognitive deficits.

Theranostics

Alexander JF,Seua AV,Arroyo LD,Ray PR,Wangzhou A,Heiβ-Lückemann L,Schedlowski M,Price TJ,Kavelaars A,Heijnen CJ

Published figure using LYVE1 monoclonal antibody (Product # 53-0443-82) in Immunocytochemistry

Tue Jul 27 00:00:00 EDT 2021

Interleukin-11-expressing fibroblasts have a unique gene signature correlated with poor prognosis of colorectal cancer.

Nature communications

Nishina T,Deguchi Y,Ohshima D,Takeda W,Ohtsuka M,Shichino S,Ueha S,Yamazaki S,Kawauchi M,Nakamura E,Nishiyama C,Kojima Y,Adachi-Akahane S,Hasegawa M,Nakayama M,Oshima M,Yagita H,Shibuya K,Mikami T,Inohara N,Matsushima K,Tada N,Nakano H

Published figure using LYVE1 monoclonal antibody (Product # 53-0443-82) in Flow Cytometry

Fri Apr 16 00:00:00 EDT 2021

Extracellular ATP Limits Homeostatic T Cell Migration Within Lymph Nodes.

Frontiers in immunology

Kobayashi D,Sugiura Y,Umemoto E,Takeda A,Ueta H,Hayasaka H,Matsuzaki S,Katakai T,Suematsu M,Hamachi I,Yegutkin GG,Salmi M,Jalkanen S,Miyasaka M

Published figure using LYVE1 monoclonal antibody (Product # 53-0443-82) in Immunohistochemistry

Mon Feb 14 00:00:00 EST 2022

Targeting local lymphatics to ameliorate heterotopic ossification via FGFR3-BMPR1a pathway.

Nature communications

Zhang D,Huang J,Sun X,Chen H,Huang S,Yang J,Du X,Tan Q,Luo F,Zhang R,Zhou S,Jiang W,Ni Z,Wang Z,Jin M,Xu M,Li F,Chen L,Liu M,Su N,Luo X,Yin L,Zhu Y,Feng JQ,Chen D,Qi H,Chen L,Xie Y

Published figure using LYVE1 monoclonal antibody (Product # 53-0443-82) in Immunocytochemistry

Mon Jul 19 00:00:00 EDT 2021

3-hydroxy-L-kynurenamine is an immunomodulatory biogenic amine.

Nature communications

Clement CC,D'Alessandro A,Thangaswamy S,Chalmers S,Furtado R,Spada S,Mondanelli G,Ianni F,Gehrke S,Gargaro M,Manni G,Cara LCL,Runge P,Tsai WL,Karaman S,Arasa J,Fernandez-Rodriguez R,Beck A,Macchiarulo A,Gadina M,Halin C,Fallarino F,Skobe M,Veldhoen M,Moretti S,Formenti S,Demaria S,Soni RK,Galarini R,Sardella R,Lauvau G,Putterman C,Alitalo K,Grohmann U,Santambrogio L

53-0443-82 was used in Flow Cytometry to show tryptophan-mediated immune tolerance is dependent on the amine 3-hydroxy-L-kynurenamine.

Wed Jul 21 00:00:00 EDT 2021

Meningeal lymphatic vessels regulate brain tumor drainage and immunity.

Cell research

Hu X,Deng Q,Ma L,Li Q,Chen Y,Liao Y,Zhou F,Zhang C,Shao L,Feng J,He T,Ning W,Kong Y,Huo Y,He A,Liu B,Zhang J,Adams R,He Y,Tang F,Bian X,Luo J

Published figure using LYVE1 monoclonal antibody (Product # 53-0443-82) in Flow Cytometry

Sun Mar 01 00:00:00 EST 2020

Meningeal lymphatic vessels regulate brain tumor drainage and immunity.

Cell research

Hu X,Deng Q,Ma L,Li Q,Chen Y,Liao Y,Zhou F,Zhang C,Shao L,Feng J,He T,Ning W,Kong Y,Huo Y,He A,Liu B,Zhang J,Adams R,He Y,Tang F,Bian X,Luo J

Published figure using LYVE1 monoclonal antibody (Product # 53-0443-82) in Flow Cytometry

Sun Mar 01 00:00:00 EST 2020

Tissue-resident macrophages in omentum promote metastatic spread of ovarian cancer.

The Journal of experimental medicine

Etzerodt A,Moulin M,Doktor TK,Delfini M,Mossadegh-Keller N,Bajenoff M,Sieweke MH,Moestrup SK,Auphan-Anezin N,Lawrence T

53-0443 was used in Flow cytometry/Cell sorting to describe a specific role for tissue-resident macrophages in the invasive progression of metastatic ovarian cancer.

Mon Apr 06 00:00:00 EDT 2020

Tissue-resident macrophages in omentum promote metastatic spread of ovarian cancer.

The Journal of experimental medicine

Etzerodt A,Moulin M,Doktor TK,Delfini M,Mossadegh-Keller N,Bajenoff M,Sieweke MH,Moestrup SK,Auphan-Anezin N,Lawrence T

53-0443 was used in Flow cytometry/Cell sorting to describe a specific role for tissue-resident macrophages in the invasive progression of metastatic ovarian cancer.

Mon Apr 06 00:00:00 EDT 2020

FK506 induces lung lymphatic endothelial cell senescence and downregulates LYVE-1 expression, with associated decreased hyaluronan uptake.

Molecular medicine (Cambridge, Mass.)

Shrestha S,Cho W,Stump B,Imani J,Lamattina AM,Louis PH,Pazzanese J,Rosas IO,Visner G,Perrella MA,El-Chemaly S

Published figure using LYVE1 monoclonal antibody (Product # 53-0443-82) in Flow Cytometry

Fri Jul 31 00:00:00 EDT 2020

Anatomic evidence shows that lymphatic drainage exists in the pituitary to loop the cerebral lymphatic circulation.

Medical hypotheses

Elham E,Wumaier R,Wang C,Luo X,Chen T,Zhong N

53-0443 was used in Immunohistochemistry to investigate the drainage differences between the perinasal and intracerebral lymphatic systems.

Thu Oct 01 00:00:00 EDT 2020

Anatomic evidence shows that lymphatic drainage exists in the pituitary to loop the cerebral lymphatic circulation.

Medical hypotheses

Elham E,Wumaier R,Wang C,Luo X,Chen T,Zhong N

53-0443 was used in Immunohistochemistry to investigate the drainage differences between the perinasal and intracerebral lymphatic systems.

Thu Oct 01 00:00:00 EDT 2020

Meningeal lymphatics clear erythrocytes that arise from subarachnoid hemorrhage.

Nature communications

Chen J,Wang L,Xu H,Xing L,Zhuang Z,Zheng Y,Li X,Wang C,Chen S,Guo Z,Liang Q,Wang Y

53-0443-82 was used in Immunocytochemistry to demonstrate that the meningeal lymphatics drain extravasated erythrocytes from CSF into CLNs after SAH, while suggesting that modulating this draining may offer therapeutic approaches to alleviate SAH severity.

Mon Jun 22 00:00:00 EDT 2020

Meningeal lymphatics clear erythrocytes that arise from subarachnoid hemorrhage.

Nature communications

Chen J,Wang L,Xu H,Xing L,Zhuang Z,Zheng Y,Li X,Wang C,Chen S,Guo Z,Liang Q,Wang Y

Mon Jun 22 00:00:00 EDT 2020

Epigenetic priming by Dot1l in lymphatic endothelial progenitors ensures normal lymphatic development and function.

Cell death & disease

Yoo H,Lee YJ,Park C,Son D,Choi DY,Park JH,Choi HJ,La HW,Choi YJ,Moon EH,Saur D,Chung HM,Song H,Do JT,Jang H,Lee DR,Park C,Lee OH,Cho SG,Hong SH,Kong G,Kim JH,Choi Y,Hong K

Published figure using LYVE1 monoclonal antibody (Product # 53-0443-82) in Immunocytochemistry

Mon Jan 06 00:00:00 EST 2020

Epigenetic priming by Dot1l in lymphatic endothelial progenitors ensures normal lymphatic development and function.

Cell death & disease

Yoo H,Lee YJ,Park C,Son D,Choi DY,Park JH,Choi HJ,La HW,Choi YJ,Moon EH,Saur D,Chung HM,Song H,Do JT,Jang H,Lee DR,Park C,Lee OH,Cho SG,Hong SH,Kong G,Kim JH,Choi Y,Hong K

Published figure using LYVE1 monoclonal antibody (Product # 53-0443-82) in Immunocytochemistry

Mon Jan 06 00:00:00 EST 2020

Efficient Tissue Clearing and Multi-Organ Volumetric Imaging Enable Quantitative Visualization of Sparse Immune Cell Populations During Inflammation.

Frontiers in immunology

Hofmann J,Gadjalova I,Mishra R,Ruland J,Keppler SJ

Published figure using LYVE1 monoclonal antibody (Product # 53-0443-82) in Immunocytochemistry

Wed Jun 16 00:00:00 EDT 2021

Meningeal lymphatic dysfunction exacerbates traumatic brain injury pathogenesis.

Nature communications

Bolte AC,Dutta AB,Hurt ME,Smirnov I,Kovacs MA,McKee CA,Ennerfelt HE,Shapiro D,Nguyen BH,Frost EL,Lammert CR,Kipnis J,Lukens JR

53-0443-82 was used in Immunohistochemistry to provide insights into both the causes and consequences of meningeal lymphatic dysfunction in TBI and suggest that therapeutics targeting the meningeal lymphatic system may offer strategies to treat TBI.

Thu Sep 10 00:00:00 EDT 2020

Lymph Node Mesenchymal and Endothelial Stromal Cells Cooperate via the RANK-RANKL Cytokine Axis to Shape the Sinusoidal Macrophage Niche.

Immunity

Camara A,Cordeiro OG,Alloush F,Sponsel J,Chypre M,Onder L,Asano K,Tanaka M,Yagita H,Ludewig B,Flacher V,Mueller CG

53-0443 was used in Immunohistochemistry-immunofluorescence to demonstrate that mesenchymal stromal cells created a niche environment with LECs for specialized macrophages to take up residence, complete their differentiation program, and fulfill their immune function.

Tue Jun 18 00:00:00 EDT 2019

Complex Non-sinus-associated Pachymeningeal Lymphatic Structures: Interrelationship With Blood Microvasculature.

Frontiers in physiology

Glinskii OV,Huxley VH,Xie L,Bunyak F,Palaniappan K,Glinsky VV

Published figure using LYVE1 monoclonal antibody (Product # 53-0443-82) in Immunofluorescence

Tue Sep 29 00:00:00 EDT 2020

CNS lymphatic drainage and neuroinflammation are regulated by meningeal lymphatic vasculature.

Nature neuroscience

Louveau A,Herz J,Alme MN,Salvador AF,Dong MQ,Viar KE,Herod SG,Knopp J,Setliff JC,Lupi AL,Da Mesquita S,Frost EL,Gaultier A,Harris TH,Cao R,Hu S,Lukens JR,Smirnov I,Overall CC,Oliver G,Kipnis J

53-0443 was used in Immunohistochemistry to demonstrate that meningeal lymphatics fulfill two critical criteria: they assist in the drainage of cerebrospinal fluid components and enable immune cells to enter draining lymph nodes in a CCR7-dependent manner.

Mon Oct 01 00:00:00 EDT 2018

The transcriptomic and epigenetic map of vascular quiescence in the continuous lung endothelium.

eLife

Schlereth K,Weichenhan D,Bauer T,Heumann T,Giannakouri E,Lipka D,Jaeger S,Schlesner M,Aloy P,Eils R,Plass C,Augustin HG

53-0443 was used in Flow cytometry/Cell sorting to establish the transcriptional and epigenetic landscape of vascular quiescence in the continuous lung endothelium.

Fri May 11 00:00:00 EDT 2018

Lymphatic Endothelial Cells Produce M-CSF, Causing Massive Bone Loss in Mice.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Wang W,Wang H,Zhou X,Li X,Sun W,Dellinger M,Boyce BF,Xing L

Published figure using LYVE1 monoclonal antibody (Product # 53-0443-82) in Flow Cytometry

Mon May 01 00:00:00 EDT 2017

Lymphatic Endothelial Cells Produce M-CSF, Causing Massive Bone Loss in Mice.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Wang W,Wang H,Zhou X,Li X,Sun W,Dellinger M,Boyce BF,Xing L

Published figure using LYVE1 monoclonal antibody (Product # 53-0443-82) in Flow Cytometry

Mon May 01 00:00:00 EDT 2017

Long-term consequences of topical dexamethasone treatment during acute corneal HSV-1 infection on the immune system.

Journal of leukocyte biology

Chucair-Elliott AJ,Carr MM,Carr DJJ

Published figure using LYVE1 monoclonal antibody (Product # 53-0443-82) in Immunofluorescence

Mon May 01 00:00:00 EDT 2017

Long-term consequences of topical dexamethasone treatment during acute corneal HSV-1 infection on the immune system.

Journal of leukocyte biology

Chucair-Elliott AJ,Carr MM,Carr DJJ

Published figure using LYVE1 monoclonal antibody (Product # 53-0443-82) in Immunofluorescence

Mon May 01 00:00:00 EDT 2017

Defective lymphatic valve development and chylothorax in mice with a lymphatic-specific deletion of Connexin43.

Developmental biology

Munger SJ,Davis MJ,Simon AM

Published figure using LYVE1 monoclonal antibody (Product # 53-0443-82) in Immunohistochemistry

Sun Jan 15 00:00:00 EST 2017

Induction of Immune Surveillance of the Dysmorphogenic Lens.

Scientific reports

Logan CM,Bowen CJ,Menko AS

53-0443 was used in Immunohistochemistry to demonstrate that lens degeneration induces an immune response that can contribute to the fibrosis that often accompanies lens dysgenesis in mice.

Fri Nov 24 00:00:00 EST 2017

Differential DARC/ACKR1 expression distinguishes venular from non-venular endothelial cells in murine tissues.

BMC biology

Thiriot A,Perdomo C,Cheng G,Novitzky-Basso I,McArdle S,Kishimoto JK,Barreiro O,Mazo I,Triboulet R,Ley K,Rot A,von Andrian UH

53-0443 was used in Immunohistochemistry-immunofluorescence to characterise DARC (Duffy Antigen Receptor for Chemokines) expression and distribution in the microvasculature of murine tissues.

Fri May 19 00:00:00 EDT 2017

Lymphatic endothelial cells efferent to inflamed joints produce iNOS and inhibit lymphatic vessel contraction and drainage in TNF-induced arthritis in mice.

Arthritis research & therapy

Liang Q,Ju Y,Chen Y,Wang W,Li J,Zhang L,Xu H,Wood RW,Schwarz EM,Boyce BF,Wang Y,Xing L

Published figure using LYVE1 monoclonal antibody (Product # 53-0443-82) in Flow Cytometry

Sat Mar 12 00:00:00 EST 2016

Segregated Foxc2, NFATc1 and Connexin expression at normal developing venous valves, and Connexin-specific differences in the valve phenotypes of Cx37, Cx43, and Cx47 knockout mice.

Developmental biology

Munger SJ,Geng X,Srinivasan RS,Witte MH,Paul DL,Simon AM

Published figure using LYVE1 monoclonal antibody (Product # 53-0443-82) in Immunofluorescence

Fri Apr 15 00:00:00 EDT 2016

Diphtheria toxin-mediated ablation of lymphatic endothelial cells results in progressive lymphedema.

JCI insight

Gardenier JC,Hespe GE,Kataru RP,Savetsky IL,Torrisi JS,Nores GDG,Dayan JJ,Chang D,Zampell J,Martínez-Corral I,Ortega S,Mehrara BJ

Published figure using LYVE1 monoclonal antibody (Product # 53-0443-82) in Flow Cytometry

Thu Sep 22 00:00:00 EDT 2016

Treg engage lymphotoxin beta receptor for afferent lymphatic transendothelial migration.

Nature communications

Brinkman CC,Iwami D,Hritzo MK,Xiong Y,Ahmad S,Simon T,Hippen KL,Blazar BR,Bromberg JS

53-0443 was used in Immunohistochemistry-wholemount to demonstrate a form of T-cell migration used only by Treg in tissues that serves an important role in their suppressive function.

Tue Jun 21 00:00:00 EDT 2016

Integrin-Alpha IIb Identifies Murine Lymph Node Lymphatic Endothelial Cells Responsive to RANKL.

PloS one

Cordeiro OG,Chypre M,Brouard N,Rauber S,Alloush F,Romera-Hernandez M,Bénézech C,Li Z,Eckly A,Coles MC,Rot A,Yagita H,Léon C,Ludewig B,Cupedo T,Lanza F,Mueller CG

53-0443 was used in Immunofluorescence to demonstrate that stromal reticular cells activate lymphatic endothelial cells via RANKL and support the action of hematopoietic cell-derived lymphotoxin.

Thu Aug 11 00:00:00 EDT 2016

The Lymphatic Endothelial mCLCA1 Antibody Induces Proliferation and Growth of Lymph Node Lymphatic Sinuses.

PloS one

Jordan-Williams KL,Ramanujam N,Farr AG,Ruddell A

53-0443 was used in Immunofluorescence to investigate the induction of proliferation and growth of lymph node lymphatic sinuses.

Mon Jul 10 00:00:00 EDT 2017

Structural and functional features of central nervous system lymphatic vessels.

Nature

Louveau A,Smirnov I,Keyes TJ,Eccles JD,Rouhani SJ,Peske JD,Derecki NC,Castle D,Mandell JW,Lee KS,Harris TH,Kipnis J

53-0443 was used in Immunohistochemistry to investigate the mechanisms governing the entrance and exit of immune cells from the CNS.

Thu Jul 16 00:00:00 EDT 2015

Lymph node stromal cells constrain immunity via MHC class II self-antigen presentation.

eLife

Baptista AP,Roozendaal R,Reijmers RM,Koning JJ,Unger WW,Greuter M,Keuning ED,Molenaar R,Goverse G,Sneeboer MM,den Haan JM,Boes M,Mebius RE

53-0443 was used in Immunofluorescence on frozen tissues to investigate the role of MHC-II in lymph node stromal cells, showing that stromal cells constrain immunity through MHC-II expression.

Wed Nov 19 00:00:00 EST 2014

Hepatic stellate cells promote tumor progression by enhancement of immunosuppressive cells in an orthotopic liver tumor mouse model.

Laboratory investigation; a journal of technical methods and pathology

Zhao W,Zhang L,Xu Y,Zhang Z,Ren G,Tang K,Kuang P,Zhao B,Yin Z,Wang X

53-0443 was used in Immunofluorescence to study the complex tumor-host interactions in hepatocellular carcinoma, specifically how the immunosuppressive properties of hepatic stellate cells may contribute to hepatocellular carcinoma occurrence and development..

Sat Feb 01 00:00:00 EST 2014

Transcriptional profiling of stroma from inflamed and resting lymph nodes defines immunological hallmarks.

Nature immunology

Malhotra D,Fletcher AL,Astarita J,Lukacs-Kornek V,Tayalia P,Gonzalez SF,Elpek KG,Chang SK,Knoblich K,Hemler ME,Brenner MB,Carroll MC,Mooney DJ,Turley SJ

53-0443 was used in Flow cytometry/Cell sorting to compare transcriptional characteristics of mouse lymph node stromal cell subsets.

Sun Apr 01 00:00:00 EDT 2012

Connexin37 and Connexin43 deficiencies in mice disrupt lymphatic valve development and result in lymphatic disorders including lymphedema and chylothorax.

Developmental biology

Kanady JD,Dellinger MT,Munger SJ,Witte MH,Simon AM

53-0443 was used in Immunohistochemistry to show that at least three Cxs are expressed in the developing lymphatic vasculature and, when defective, are associated with clinically manifest lymphatic disorders in mice and man.

Wed Jun 15 00:00:00 EDT 2011

Identification of targets of Prox1 during in vitro vascular differentiation from embryonic stem cells: functional roles of HoxD8 in lymphangiogenesis.

Journal of cell science

Harada K,Yamazaki T,Iwata C,Yoshimatsu Y,Sase H,Mishima K,Morishita Y,Hirashima M,Oike Y,Suda T,Miura N,Watabe T,Miyazono K

Sun Nov 01 00:00:00 EDT 2009

Hematopoietic stem cells contribute to lymphatic endothelium.

PloS one

Jiang S,Bailey AS,Goldman DC,Swain JR,Wong MH,Streeter PR,Fleming WH

Published figure using LYVE1 monoclonal antibody (Product # 53-0443-82) in Immunofluorescence

Wed Feb 04 00:00:00 EST 2009

Bioinformatics

Protein Aliases: cell surface retention sequence binding protein-1; Cell surface retention sequence-binding protein 1; CRSBP-1; extra cellular link domain-containing 1; Extracellular link domain-containing protein 1; lymphatic vessel endothelial HA receptor-1; lymphatic vessel endothelial HA recptor-1; Lymphatic vessel endothelial hyaluronic acid receptor 1; LYVE-1; sLyve 1; sLyve1; soluble LYVE 1; soluble LYVE1

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Gene Aliases: 1200012G08Rik; Crsbp-1; Crsbp1; Lyve-1; Lyve1; Xlkd1

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UniProt ID: (Mouse) Q8BHC0

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Entrez Gene ID: (Mouse) 114332

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Function(s)

transmembrane signaling receptor activity hyaluronic acid binding transmembrane signal receptor

Process(es)

glycosaminoglycan catabolic process transport cell adhesion signal transduction

Performance Guarantee

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LYVE1 Monoclonal Antibody (ALY7), Alexa Fluor™ 488, eBioscience™

LYVE1 Antibody (53-0443-82) in IHC (F)

Product Details

53-0443-82

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

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RRID

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