Invitrogen

CD95 (APO-1/Fas) Monoclonal Antibody (15A7), eBioscience™

CD95 (APO-1/Fas) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 18

CD95 (APO-1/Fas) Antibody (14-0951-85) in Flow

Staining of BALB/c thymocytes with 0.5 µg of Mouse IgG1 kappa Isotype Control Purified (cat. 14-4714) (open histogram) or 0.5 µg of Anti-Mouse CD95 (APO-1/Fas) Purified (filled histogram) followed by Anti-Mouse IgG FITC (cat. 11-4011). Total viable cells were used for analysis.

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Product Details

14-0951-85

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/test

View 22 publications 22 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

15A7

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_467393

Product Specific Information

Description: The 15A7 monoclonal antibody reacts with mouse CD95 (Fas, Apo-1), a 40-50 kDa member of the TNFR superfamily. CD95 is expressed by a broad range of hematopoietic and non-hematopoietic cells including monocytes, neutrophils, activated lymphocytes and fibroblasts. Interaction of CD95 on mature lymphocytes with its ligand (FasL) induces apoptosis and is thought to be important in peripheral tolerance. 15A7 reacts with mouse CD95 in BALB/c strain and a bit more weakly in C57Bl/6 strain. 15A7 does not induce apoptosis of Fas-expressing cells.

Applications Reported: The 15A7 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 15A7 antibody has been tested by flow cytometric analysis of BALB/c splenocytes and thymocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

FAS (Fas, CD95, APO-1) is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor of the TNFR (tumor necrosis factor receptor) superfamily. FAS also exists in a soluble form that weight approximately 26kDa. Stimulation of FAS results in aggregation of its intracellular FAS-associated death domains (FADD), formation of the death-inducing signaling complex (DISC) and activation of caspases. The auto-proteolytic processing of the caspases in the complex trigger a downstream cascade that leads to apoptosis. FAS has been shown to activate NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and is involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described. The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full-length isoform. The Fas/Fas ligand system has been shown to play a role in a number of human diseases such as AIDS, hepatitis and cancer. It is also assumed that induction of apoptosis through FAS ligand pathway is predominantly involved in anti-viral immune responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CD4⁺ CTLs Act as a Key Effector Population for Allograft Rejection of MSCs in a Donor MHC-II Dependent Manner in Injured Liver.

Aging and disease

Shen S,Li Y,Jin M,Fan D,Pan R,Lin A,Chen Y,Xiang L,Zhao RC,Shao J

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 14-0951-81) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2022

Elimination of receptor binding by influenza hemagglutinin improves vaccine-induced immunity.

NPJ vaccines

Hendin HE,Lavoie PO,Gravett JM,Pillet S,Saxena P,Landry N,D'Aoust MA,Ward BJ

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 14-0951-81) in Flow Cytometry

Mon Apr 11 00:00:00 EDT 2022

Early innate and adaptive immune perturbations determine long-term severity of chronic virus and Mycobacterium tuberculosis coinfection.

Immunity

Xu W,Snell LM,Guo M,Boukhaled G,Macleod BL,Li M,Tullius MV,Guidos CJ,Tsao MS,Divangahi M,Horwitz MA,Liu J,Brooks DG

14-0951-85 was used in Miscellaneous to examine how chronic viral infections alter the pulmonary microenvironment to foster coinfection and worsen disease severity.

Tue Mar 09 00:00:00 EST 2021

The ubiquitin ligase Peli1 inhibits ICOS and thereby Tfh-mediated immunity.

Cellular & molecular immunology

Huang X,Hao S,Liu J,Huang Y,Liu M,Xiao C,Wang Y,Pei S,Yu T,Xu J,Wang H,Dai D,Su X,Xiao Y

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 14-0951-81) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Berberine modulates hyper-inflammation in mouse macrophages stimulated with polyinosinic-polycytidylic acid via calcium-CHOP/STAT pathway.

Scientific reports

Kim HJ,Kim YJ,Park W

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 14-0951-81) in Flow Cytometry

Fri May 28 00:00:00 EDT 2021

Antigen-presenting ILC3 regulate T cell-dependent IgA responses to colonic mucosal bacteria.

The Journal of experimental medicine

Melo-Gonzalez F,Kammoun H,Evren E,Dutton EE,Papadopoulou M,Bradford BM,Tanes C,Fardus-Reid F,Swann JR,Bittinger K,Mabbott NA,Vallance BA,Willinger T,Withers DR,Hepworth MR

14-0951-85 was used in Flow Cytometry to study the mechanisms that modulate the magnitude and quality of T cell-dependent IgA responses.

Mon Apr 01 00:00:00 EDT 2019

Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells.

Cell

Gaya M,Barral P,Burbage M,Aggarwal S,Montaner B,Warren Navia A,Aid M,Tsui C,Maldonado P,Nair U,Ghneim K,Fallon PG,Sekaly RP,Barouch DH,Shalek AK,Bruckbauer A,Strid J,Batista FD

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 14-0951-81) in Flow Cytometry

Thu Jan 25 00:00:00 EST 2018

Peli1 negatively regulates noncanonical NF-κB signaling to restrain systemic lupus erythematosus.

Nature communications

Liu J,Huang X,Hao S,Wang Y,Liu M,Xu J,Zhang X,Yu T,Gan S,Dai D,Luo X,Lu Q,Mao C,Zhang Y,Shen N,Li B,Huang M,Zhu X,Jin J,Cheng X,Sun SC,Xiao Y

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 14-0951-81) in Flow Cytometry

Mon Mar 19 00:00:00 EDT 2018

Caveolin-1-dependent nanoscale organization of the BCR regulates B cell tolerance.

Nature immunology

Minguet S,Kläsener K,Schaffer AM,Fiala GJ,Osteso-Ibánez T,Raute K,Navarro-Lérida I,Hartl FA,Seidl M,Reth M,Del Pozo MA

14-0951 was used in Flow cytometry/Cell sorting to study the role of Caveolin-1 in controlling the distribution of nanoclusters of isotype-specific B cell antigen receptors on the surface of B cells.

Sun Oct 01 00:00:00 EDT 2017

NCR1-deficiency diminishes the generation of protective murine cytomegalovirus antibodies by limiting follicular helper T-cell maturation.

European journal of immunology

Miletic A,Lenartic M,Popovic B,Brizic I,Trsan T,Miklic K,Mandelboim O,Krmpotic A,Jonjic S

14-0951 was used in Flow cytometry/Cell sorting to provide evidence for the role of NCR1 in antibody response to mouse cytomegalovirus infection.

Fri Sep 01 00:00:00 EDT 2017

Armored CAR T cells enhance antitumor efficacy and overcome the tumor microenvironment.

Scientific reports

Yeku OO,Purdon TJ,Koneru M,Spriggs D,Brentjens RJ

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 14-0951-81) in Flow Cytometry

Tue Sep 05 00:00:00 EDT 2017

YY1 plays an essential role at all stages of B-cell differentiation.

Proceedings of the National Academy of Sciences of the United States of America

Kleiman E,Jia H,Loguercio S,Su AI,Feeney AJ

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 14-0951-81) in Flow Cytometry

Tue Jul 05 00:00:00 EDT 2016

Liver sinusoidal endothelial cells induce tolerance of autoreactive CD4+ recent thymic emigrants.

Scientific reports

Xu X,Jin R,Li M,Wang K,Zhang S,Hao J,Sun X,Zhang Y,Wu H,Zhang J,Ge Q

14-0951 was used in Flow cytometry/Cell sorting to investigate the tolerance of liver-persisting CD4(+) recent thymic emigrants.

Fri Jan 22 00:00:00 EST 2016

CRISPR-Mediated Triple Knockout of SLAMF1, SLAMF5 and SLAMF6 Supports Positive Signaling Roles in NKT Cell Development.

PloS one

Huang B,Gomez-Rodriguez J,Preite S,Garrett LJ,Harper UL,Schwartzberg PL

14-0951 was used in Flow cytometry/Cell sorting to elucidate the contribution of the SLAM receptors in immunodysregulation observed in X-linked lymphoproliferative syndrome Type 1.

Mon Jul 24 00:00:00 EDT 2017

LEF-1 and TCF-1 orchestrate T(FH) differentiation by regulating differentiation circuits upstream of the transcriptional repressor Bcl6.

Nature immunology

Choi YS,Gullicksrud JA,Xing S,Zeng Z,Shan Q,Li F,Love PE,Peng W,Xue HH,Crotty S

14-0951 was used in Flow cytometry/Cell sorting to report that selective loss of Lef1 or Tcf7 results in T(FH) cell defects.

Tue Sep 01 00:00:00 EDT 2015

IDO1 suppresses inhibitor development in hemophilia A treated with factor VIII.

The Journal of clinical investigation

Matino D,Gargaro M,Santagostino E,Di Minno MN,Castaman G,Morfini M,Rocino A,Mancuso ME,Di Minno G,Coppola A,Talesa VN,Volpi C,Vacca C,Orabona C,Iannitti R,Mazzucconi MG,Santoro C,Tosti A,Chiappalupi S,Sorci G,Tagariello G,Belvini D,Radossi P,Landolfi R,Fuchs D,Boon L,Pirro M,Marchesini E,Grohmann U,Puccetti P,Iorio A,Fallarino F

14-0951 was used in Flow cytometry/Cell sorting to investigate the association between IDO1 competence and inhibitor status in haemophilia A patients.

Thu Oct 01 00:00:00 EDT 2015

STIM1 controls T cell-mediated immune regulation and inflammation in chronic infection.

The Journal of clinical investigation

Desvignes L,Weidinger C,Shaw P,Vaeth M,Ribierre T,Liu M,Fergus T,Kozhaya L,McVoy L,Unutmaz D,Ernst JD,Feske S

14-0951 was used in Flow cytometry/Cell sorting to study the role of the activator stromal interaction molecule 1 (STIM1) on T cell regulation during chronic Mycobacterium tuberculosis infection.

Mon Jun 01 00:00:00 EDT 2015

T follicular helper, but not Th1, cell differentiation in the absence of conventional dendritic cells.

Journal of immunology (Baltimore, Md. : 1950)

Dahlgren MW,Gustafsson-Hedberg T,Livingston M,Cucak H,Alsén S,Yrlid U,Johansson-Lindbom B

14-0951 was used in Flow cytometry/Cell sorting to investigate the accessory role of conventional DCs during the concurrent development of Tfh and effector Th1 cells.

Mon Jun 01 00:00:00 EDT 2015

GM-CSF in the lung protects against lethal influenza infection.

American journal of respiratory and critical care medicine

Huang FF,Barnes PF,Feng Y,Donis R,Chroneos ZC,Idell S,Allen T,Perez DR,Whitsett JA,Dunussi-Joannopoulos K,Shams H

14-0951 was used in Flow cytometry/Cell sorting to determine if increased expression of granulocyte/macrophage colony-stimulating factor in the lung increases resistance to influenza.

Fri Jul 15 00:00:00 EDT 2011

The mismatch repair pathway functions normally at a non-AID target in germinal center B cells.

Blood

Green B,Belcheva A,Nepal RM,Boulianne B,Martin A

14-0951 was used in Flow cytometry/Cell sorting to investigate whether mismatch repair corrects mutations in germinal center B cells at a gene that is not mutated by AID.

Thu Sep 15 00:00:00 EDT 2011

Rescue of tumor-infiltrating lymphocytes from activation-induced cell death enhances the antitumor CTL response in CD5-deficient mice.

Journal of immunology (Baltimore, Md. : 1950)

Tabbekh M,Franciszkiewicz K,Haouas H,Lécluse Y,Benihoud K,Raman C,Mami-Chouaib F

14-0951-85 was used in Flow Cytometry to compare the T cell response to the B16F10 melanoma engrafted into CD5-deficient and wild-type C57BL/6 mice.

Fri Jul 01 00:00:00 EDT 2011

Constitutive CD40L expression on B cells prematurely terminates germinal center response and leads to augmented plasma cell production in T cell areas.

Journal of immunology (Baltimore, Md. : 1950)

Bolduc A,Long E,Stapler D,Cascalho M,Tsubata T,Koni PA,Shimoda M

14-0951 was used in Flow cytometry/Cell sorting to elucidate the role of CD40 signalling through cognate T-B interaction in the generation of germinal centre and memory B cells.

Thu Jul 01 00:00:00 EDT 2010

AID constrains germinal center size by rendering B cells susceptible to apoptosis.

Blood

Zaheen A,Boulianne B,Parsa JY,Ramachandran S,Gommerman JL,Martin A

14-0951 was used in Flow cytometry/Cell sorting to report a link between AID-induced DNA damage and B-cell apoptosis that has implications for the development of B-cell disorders.

Thu Jul 16 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: Apo-1 antigen; Apo-1 Fas; Apo1 antigen; Apoptosis-mediating surface antigen FAS; CD95; Fas (TNF receptor superfamily member); Fas antigen; FASLG receptor; lymphoproliferation; OTTHUMP00000020045; OTTHUMP00000020046; OTTHUMP00000020051; OTTHUMP00000059646; Tumor necrosis factor receptor superfamily member 6

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Gene Aliases: AI196731; APO1; APT1; CD95; Fas; lpr; TNFR6; Tnfrsf6

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UniProt ID: (Mouse) P25446

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Entrez Gene ID: (Mouse) 14102

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Function(s)

protease binding transmembrane signaling receptor activity tumor necrosis factor-activated receptor activity protein binding kinase binding protein complex binding identical protein binding transmembrane signal receptor

Process(es)

immunoglobulin production renal system process apoptotic process activation-induced cell death of T cells inflammatory cell apoptotic process obsolete transformed cell apoptotic process immune response signal transduction extrinsic apoptotic signaling pathway via death domain receptors response to toxic substance gene expression regulation of gene expression positive regulation of cell death B cell mediated immunity dendrite regeneration positive regulation of protein homooligomerization tumor necrosis factor-mediated signaling pathway regulation of cell proliferation regulation of apoptotic process T cell homeostasis positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of cysteine-type endopeptidase activity involved in apoptotic process negative thymic T cell selection regulation of lymphocyte differentiation regulation of myeloid cell differentiation spleen development negative regulation of B cell activation protein homooligomerization response to glucocorticoid neuron apoptotic process cellular response to lithium ion cellular response to hyperoxia positive regulation of release of cytochrome c from mitochondria motor neuron apoptotic process apoptotic signaling pathway extrinsic apoptotic signaling pathway extrinsic apoptotic signaling pathway in absence of ligand hepatocyte apoptotic process necroptotic signaling pathway positive regulation of extrinsic apoptotic signaling pathway in absence of ligand

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CD95 (APO-1/Fas) Monoclonal Antibody (15A7), eBioscience™

CD95 (APO-1/Fas) Antibody (14-0951-85) in Flow

Product Details

14-0951-85

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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