Invitrogen

CD95 (APO-1/Fas) Monoclonal Antibody (15A7), PerCP-eFluor™ 710, eBioscience™

20 Published Figures

12 References

View all (107) FAS (CD95) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (20)

CD95 (APO-1/Fas) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 21

CD95 (APO-1/Fas) Antibody (46-0951-82) in Flow

Staining of C57Bl/6 thymocytes with 0.5 µg of Mouse IgG1 K Isotype Control PerCP-eFluor® 710 (Product # 46-4714-82) (blue histogram) or 0.5 µg of Anti-Mouse CD95 (APO-1/Fas) PerCP-eFluor® 710 (purple histogram). Total viable cells were used for analysis.

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Product Details

46-0951-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/test

View 12 publications 12 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-eFluor™ 710, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

15A7

Conjugate

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

Excitation/Emission Max

482/708 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573700

Product Specific Information

Description: The 15A7 monoclonal antibody reacts with mouse CD95 (Fas, Apo-1), a 40-50 kDa member of the TNFR superfamily. CD95 is expressed by a broad range of hematopoietic and non-hematopoietic cells including monocytes, neutrophils, activated lymphocytes and fibroblasts. Interaction of CD95 on mature lymphocytes with its ligand (FasL) induces apoptosis and is thought to be important in peripheral tolerance. 15A7 reacts with mouse CD95 in BALB/c strain and a bit more weakly in C57Bl/6 strain. 15A7 does not induce apoptosis of Fas-expressing cells.

Applications Reported: This 15A7 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 15A7 antibody has been tested by flow cytometric analysis of mouse thymocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

FAS (Fas, CD95, APO-1) is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor of the TNFR (tumor necrosis factor receptor) superfamily. FAS also exists in a soluble form that weight approximately 26kDa. Stimulation of FAS results in aggregation of its intracellular FAS-associated death domains (FADD), formation of the death-inducing signaling complex (DISC) and activation of caspases. The auto-proteolytic processing of the caspases in the complex trigger a downstream cascade that leads to apoptosis. FAS has been shown to activate NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and is involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described. The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full-length isoform. The Fas/Fas ligand system has been shown to play a role in a number of human diseases such as AIDS, hepatitis and cancer. It is also assumed that induction of apoptosis through FAS ligand pathway is predominantly involved in anti-viral immune responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

CD4⁺ CTLs Act as a Key Effector Population for Allograft Rejection of MSCs in a Donor MHC-II Dependent Manner in Injured Liver.

Aging and disease

Shen S,Li Y,Jin M,Fan D,Pan R,Lin A,Chen Y,Xiang L,Zhao RC,Shao J

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 46-0951-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2022

Elimination of receptor binding by influenza hemagglutinin improves vaccine-induced immunity.

NPJ vaccines

Hendin HE,Lavoie PO,Gravett JM,Pillet S,Saxena P,Landry N,D'Aoust MA,Ward BJ

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 46-0951-82) in Flow Cytometry

Mon Apr 11 00:00:00 EDT 2022

The ubiquitin ligase Peli1 inhibits ICOS and thereby Tfh-mediated immunity.

Cellular & molecular immunology

Huang X,Hao S,Liu J,Huang Y,Liu M,Xiao C,Wang Y,Pei S,Yu T,Xu J,Wang H,Dai D,Su X,Xiao Y

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 46-0951-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Berberine modulates hyper-inflammation in mouse macrophages stimulated with polyinosinic-polycytidylic acid via calcium-CHOP/STAT pathway.

Scientific reports

Kim HJ,Kim YJ,Park W

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 46-0951-82) in Flow Cytometry

Fri May 28 00:00:00 EDT 2021

Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells.

Cell

Gaya M,Barral P,Burbage M,Aggarwal S,Montaner B,Warren Navia A,Aid M,Tsui C,Maldonado P,Nair U,Ghneim K,Fallon PG,Sekaly RP,Barouch DH,Shalek AK,Bruckbauer A,Strid J,Batista FD

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 46-0951-82) in Flow Cytometry

Thu Jan 25 00:00:00 EST 2018

Peli1 negatively regulates noncanonical NF-κB signaling to restrain systemic lupus erythematosus.

Nature communications

Liu J,Huang X,Hao S,Wang Y,Liu M,Xu J,Zhang X,Yu T,Gan S,Dai D,Luo X,Lu Q,Mao C,Zhang Y,Shen N,Li B,Huang M,Zhu X,Jin J,Cheng X,Sun SC,Xiao Y

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 46-0951-82) in Flow Cytometry

Mon Mar 19 00:00:00 EDT 2018

Armored CAR T cells enhance antitumor efficacy and overcome the tumor microenvironment.

Scientific reports

Yeku OO,Purdon TJ,Koneru M,Spriggs D,Brentjens RJ

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 46-0951-82) in Flow Cytometry

Tue Sep 05 00:00:00 EDT 2017

Class-switched anti-insulin antibodies originate from unconventional antigen presentation in multiple lymphoid sites.

The Journal of experimental medicine

Wan X,Thomas JW,Unanue ER

46-0951 was used in Flow cytometry/Cell sorting to investigate the interactions of insulin-specific T and B lymphocytes using T cell and B cell receptor transgenic mice.

Mon May 30 00:00:00 EDT 2016

YY1 plays an essential role at all stages of B-cell differentiation.

Proceedings of the National Academy of Sciences of the United States of America

Kleiman E,Jia H,Loguercio S,Su AI,Feeney AJ

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 46-0951-82) in Flow Cytometry

Tue Jul 05 00:00:00 EDT 2016

The histone lysine methyltransferase KMT2D sustains a gene expression program that represses B cell lymphoma development.

Nature medicine

Ortega-Molina A,Boss IW,Canela A,Pan H,Jiang Y,Zhao C,Jiang M,Hu D,Agirre X,Niesvizky I,Lee JE,Chen HT,Ennishi D,Scott DW,Mottok A,Hother C,Liu S,Cao XJ,Tam W,Shaknovich R,Garcia BA,Gascoyne RD,Ge K,Shilatifard A,Elemento O,Nussenzweig A,Melnick AM,Wendel HG

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 46-0951-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2015

Substituting threonine 187 with alanine in p27Kip1 prevents pituitary tumorigenesis by two-hit loss of Rb1 and enhances humoral immunity in old age.

The Journal of biological chemistry

Zhao H,Bauzon F,Bi E,Yu JJ,Fu H,Lu Z,Cui J,Jeon H,Zang X,Ye BH,Zhu L

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 46-0951-82) in Flow Cytometry

Fri Feb 27 00:00:00 EST 2015

Up-regulation of Fas (CD95) expression in tumour cells in vivo.

Immunology

Peshes-Yaloz N,Rosen D,Sondel PM,Krammer PH,Berke G

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 46-0951-82) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: Apo-1 antigen; Apo-1 Fas; Apo1 antigen; Apoptosis-mediating surface antigen FAS; CD95; Fas (TNF receptor superfamily member); Fas antigen; FASLG receptor; lymphoproliferation; OTTHUMP00000020045; OTTHUMP00000020046; OTTHUMP00000020051; OTTHUMP00000059646; Tumor necrosis factor receptor superfamily member 6

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Gene Aliases: AI196731; APO1; APT1; CD95; Fas; lpr; TNFR6; Tnfrsf6

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UniProt ID: (Mouse) P25446

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Entrez Gene ID: (Mouse) 14102

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Function(s)

protease binding transmembrane signaling receptor activity tumor necrosis factor-activated receptor activity protein binding kinase binding protein complex binding identical protein binding transmembrane signal receptor

Process(es)

immunoglobulin production renal system process apoptotic process activation-induced cell death of T cells inflammatory cell apoptotic process obsolete transformed cell apoptotic process immune response signal transduction extrinsic apoptotic signaling pathway via death domain receptors response to toxic substance gene expression regulation of gene expression positive regulation of cell death B cell mediated immunity dendrite regeneration positive regulation of protein homooligomerization tumor necrosis factor-mediated signaling pathway regulation of cell proliferation regulation of apoptotic process T cell homeostasis positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of cysteine-type endopeptidase activity involved in apoptotic process negative thymic T cell selection regulation of lymphocyte differentiation regulation of myeloid cell differentiation spleen development negative regulation of B cell activation protein homooligomerization response to glucocorticoid neuron apoptotic process cellular response to lithium ion cellular response to hyperoxia positive regulation of release of cytochrome c from mitochondria motor neuron apoptotic process apoptotic signaling pathway extrinsic apoptotic signaling pathway extrinsic apoptotic signaling pathway in absence of ligand hepatocyte apoptotic process necroptotic signaling pathway positive regulation of extrinsic apoptotic signaling pathway in absence of ligand

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD95 (APO-1/Fas) Monoclonal Antibody (15A7), PerCP-eFluor™ 710, eBioscience™

CD95 (APO-1/Fas) Antibody (46-0951-82) in Flow

Product Details

46-0951-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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