Invitrogen

CD127 Monoclonal Antibody (eBioRDR5), eBioscience™

CD127 Antibody in Immunohistochemistry (IHC)

FIGURE: 1 / 16

CD127 Antibody (14-1278-82) in IHC

Frontiers in immunology 2019 - Figure 6 Greater numbers of interleukin (IL)-7Ralpha high effector memory (EM) CD8 + T cells than IL-7Ralpha low EM CD8 + T cells in the skin. (A) Immunofluorescence staining (40x) of CD8 + T cells (green) from non-lesional (healthy, HC) or lesional atopic dermatitis skin. IL-7Ralpha + CD8 + T cells (upper panel) representing IL-7Ralpha high EM CD8 + T cells were stained with antibodies (Abs) to ... View More

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Product Details

14-1278-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

View 2 publications 2 publications

Flow Cytometry (Flow)

0.5 µg/test

View 23 publications 23 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Non-human primate, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

eBioRDR5

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_657591

Product Specific Information

Description: The eBioRDR5 monoclonal antibody reacts with human CD127 (Interleukin-7 Receptor alpha). CD127 complexes with CD132, also known as the common gamma chain (gamma c), to form the multi-functional IL-7 receptor (IL-7R). CD127 is a type I glycoprotein with a molecular weight of 75-80 kDa and is expressed by immature B cells through the early pre-B stage, by thymocytes during several stages of their development, and on most mature T cells, with transient down-regulation upon activation. Binding of IL-7 results in signal transduction which occurs through several tyrosine kinase pathways including the Jak/STAT pathway. IL-7 is indispensible for the development of lymphocytes, and the control of homeostatic proliferation of T-cells in the periphery. In addition, IL-7R signaling is know to be involved in the regulation of T cell receptor (TCR) locus rearrangement in gamma delta T cells.

Interestingly, recently it has been demonstrated that CD127 expression is down-regulated on CD4+CD25+ regulatory T cells (T regs). While the co-expression of CD4 and CD25 has become widely used as an indicator of T regs, this method of identification may also include cells without suppressive activity. It has clearly been shown that CD4+CD25+ cells that have down-regulated the expression of CD127 are significantly more highly-enriched for the regulatory T population, as defined by expression of the T reg-specific transcription factor Foxp3 and the suppressive activity of these cells, in vitro.

Binding of the eBioRDR5 monoclonal antibody to PBMCs is blocked by pre-incubation of the cells with recombinant human IL-7 (Product # 14-1079-80).

Applications Reported: This eBioRDR5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioRDR5 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD127 (Interleukin-7, IL-7) is a glycoprotein involved in the regulation of lymphopoiesis. The response of cells to CD127 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer. CD127 consists of an alpha chain and a gamma chain. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules. CD127 promotes the proliferation of precursor B cells, thymocytes, T cell progenitors, and mature CD4+ and CD8+ T cells. The biological effects of IL7 are mediated by the binding of IL7 to the specific cell surface receptor. Diseases associated with CD127 dysfunction include severe combined immunodeficiency and T cell negative/B cell negative/NK positive severe combined immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Epinephrine Production in Th17 Cells and Experimental Autoimmune Encephalitis.

Frontiers in immunology

Yang P,Tian H,Zou YR,Chambon P,Ichinose H,Honig G,Diamond B,Kim SJ

14-1278-82 was used in Flow Cytometry to show that phenylethanol N-methyltransferase (PNMT), a rate-limiting enzyme of epinephrine synthesis, is specifically expressed in vitro in differentiated TH17 cells and in tissue-resident TH17 cells.

Mon Jul 05 00:00:00 EDT 2021

Epinephrine Production in Th17 Cells and Experimental Autoimmune Encephalitis.

Frontiers in immunology

Yang P,Tian H,Zou YR,Chambon P,Ichinose H,Honig G,Diamond B,Kim SJ

Mon Jul 05 00:00:00 EDT 2021

Immunophenotype of Gastric Tumors Unveils a Pleiotropic Role of Regulatory T Cells in Tumor Development.

Cancers

Rocha S,Basto AP,Ijsselsteijn ME,Teles SP,Azevedo MM,Gonçalves G,Gullo I,Almeida GM,Maqueda JJ,Oliveira MI,Carneiro F,Barata JT,Graça L,de Miranda NFCC,Carvalho J,Oliveira C

14-1278-82 was used in Flow Cytometry to explore the impact of regulatory T cell (Tregs) in gastric cancers (GCs) with distinct histology, and whether Tregs can directly influence tumor cell behavior and GC development.

Sat Jan 23 00:00:00 EST 2021

Transdifferentiation of tumor infiltrating innate lymphoid cells during progression of colorectal cancer.

Cell research

Wang S,Qu Y,Xia P,Chen Y,Zhu X,Zhang J,Wang G,Tian Y,Ying J,Fan Z

14-1278-82 was used in Flow Cytometry to profile tumor infiltrating innate lymphoid cells in colorectal cancer at early and late stages.

Wed Jul 01 00:00:00 EDT 2020

A probiotic modulates the microbiome and immunity in multiple sclerosis.

Annals of neurology

Tankou SK,Regev K,Healy BC,Tjon E,Laghi L,Cox LM,Kivisäkk P,Pierre IV,Hrishikesh L,Gandhi R,Cook S,Glanz B,Stankiewicz J,Weiner HL

Published figure using CD127 monoclonal antibody (Product # 14-1278-82) in Flow Cytometry

Fri Jun 01 00:00:00 EDT 2018

Characterization of CD127^- CD25⁺⁺ Treg from human colostrum.

American journal of reproductive immunology (New York, N.Y. : 1989)

Cérbulo-Vázquez A,Hernández-Peláez G,Arriaga-Pizano LA,Bautista-Pérez P,Romero-Venado J,Flores-González JC,Figueroa-Damian R,Soriano-Becerril D,Mancilla-Herrera I

Published figure using CD127 monoclonal antibody (Product # 14-1278-82) in Flow Cytometry

Thu Feb 01 00:00:00 EST 2018

The Effects of High Mobility Group Box-1 Protein on Peripheral Treg/Th17 Balance in Patients with Atherosclerosis.

Acta Cardiologica Sinica

Ding JW,Zhou T,Zheng XX,Wang XA,Tong XH,Luo CY,Zhang ZQ,Yu B

Published figure using CD127 monoclonal antibody (Product # 14-1278-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

Activation of miR-21-Regulated Pathways in Immune Aging Selects against Signatures Characteristic of Memory T Cells.

Cell reports

Kim C,Hu B,Jadhav RR,Jin J,Zhang H,Cavanagh MM,Akondy RS,Ahmed R,Weyand CM,Goronzy JJ

Published figure using CD127 monoclonal antibody (Product # 14-1278-82) in Flow Cytometry

Tue Nov 20 00:00:00 EST 2018

Blocking the recruitment of naive CD4⁺ T cells reverses immunosuppression in breast cancer.

Cell research

Su S,Liao J,Liu J,Huang D,He C,Chen F,Yang L,Wu W,Chen J,Lin L,Zeng Y,Ouyang N,Cui X,Yao H,Su F,Huang JD,Lieberman J,Liu Q,Song E

Published figure using CD127 monoclonal antibody (Product # 14-1278-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

Differentially Expressed Potassium Channels Are Associated with Function of Human Effector Memory CD8⁺ T Cells.

Frontiers in immunology

Sim JH,Kim KS,Park H,Kim KJ,Lin H,Kim TJ,Shin HM,Kim G,Lee DS,Park CW,Lee DH,Kang I,Kim SJ,Cho CH,Doh J,Kim HR

Published figure using CD127 monoclonal antibody (Product # 14-1278-82) in Immunohistochemistry

Wed Nov 20 00:00:00 EST 2019

HDAC inhibition potentiates immunotherapy in triple negative breast cancer.

Oncotarget

Terranova-Barberio M,Thomas S,Ali N,Pawlowska N,Park J,Krings G,Rosenblum MD,Budillon A,Munster PN

Published figure using CD127 monoclonal antibody (Product # 14-1278-82) in Flow Cytometry

Tue Dec 26 00:00:00 EST 2017

Serial immunomonitoring of cancer patients receiving combined antagonistic anti-CD40 and chemotherapy reveals consistent and cyclical modulation of T cell and dendritic cell parameters.

BMC cancer

McDonnell AM,Cook A,Robinson BWS,Lake RA,Nowak AK

Published figure using CD127 monoclonal antibody (Product # 14-1278-82) in Flow Cytometry

Thu Jun 15 00:00:00 EDT 2017

Analysis of the cross-talk of Epstein-Barr virus-infected B cells with T cells in the marmoset.

Clinical & translational immunology

Dunham J,van Driel N,Eggen BJ,Paul C,'t Hart BA,Laman JD,Kap YS

14-1278-82 was used in Flow Cytometry to examine the ex vivo cross-talk between lymph node mononuclear cells from EAE marmosets and (semi-) autologous EBV-infected B-lymphoblastoid cell lines.

Wed Feb 01 00:00:00 EST 2017

Blockade of CD127 Exerts a Dichotomous Clinical Effect in Marmoset Experimental Autoimmune Encephalomyelitis.

Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology

Dunham J,Lee LF,van Driel N,Laman JD,Ni I,Zhai W,Tu GH,Lin JC,Bauer J,'t Hart BA,Kap YS

14127882 was used in flow cytometry to test a therapeutic antibody against CD127 in a mouse model of experimental autoimmune encephalomyelitis

Tue Mar 01 00:00:00 EST 2016

The Distribution of Human Stem Cell-like Memory T Cell in Lung Cancer.

Journal of immunotherapy (Hagerstown, Md. : 1997)

Hong H,Gu Y,Sheng SY,Lu CG,Zou JY,Wu CY

Published figure using CD127 monoclonal antibody (Product # 14-1278-82) in Flow Cytometry

Thu Jan 04 00:00:00 EST 2018

T-cell activation is an immune correlate of risk in BCG vaccinated infants.

Nature communications

Fletcher HA,Snowden MA,Landry B,Rida W,Satti I,Harris SA,Matsumiya M,Tanner R,O'Shea MK,Dheenadhayalan V,Bogardus L,Stockdale L,Marsay L,Chomka A,Harrington-Kandt R,Manjaly-Thomas ZR,Naranbhai V,Stylianou E,Darboe F,Penn-Nicholson A,Nemes E,Hatherill M,Hussey G,Mahomed H,Tameris M,McClain JB,Evans TG,Hanekom WA,Scriba TJ,McShane H

14-1278-82 was used in Flow Cytometry to identify immune characteristics that correlate with an increased risk of tuberculosis contraction using trial and study data.

Tue Apr 12 00:00:00 EDT 2016

TLR7 induces anergy in human CD4(+) T cells.

Nature immunology

Dominguez-Villar M,Gautron AS,de Marcken M,Keller MJ,Hafler DA

14-1278 was used in Flow cytometry/Cell sorting to elucidate a previously unknown function for microbial pattern recognition receptors to down-regulate immune responses.

Thu Jan 01 00:00:00 EST 2015

Superiority of rapamycin over tacrolimus in preserving nonhuman primate Treg half-life and phenotype after adoptive transfer.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Singh K,Stempora L,Harvey RD,Kirk AD,Larsen CP,Blazar BR,Kean LS

14-1278 was used in Flow cytometry/Cell sorting to suggest that rapamycin may be a necessary addition to Treg immunotherapy, and that tacrolimus may be deleterious to Treg integrity posttransfer.

Mon Dec 01 00:00:00 EST 2014

Enhanced suppressor function of TIM-3+ FoxP3+ regulatory T cells.

European journal of immunology

Gautron AS,Dominguez-Villar M,de Marcken M,Hafler DA

Published figure using CD127 monoclonal antibody (Product # 14-1278-82) in Flow Cytometry

Mon Sep 01 00:00:00 EDT 2014

CD20+ B cell depletion alters T cell homing.

Journal of immunology (Baltimore, Md. : 1950)

Kap YS,van Driel N,Laman JD,Tak PP,'t Hart BA

14-1278 was used in Flow cytometry/Cell sorting to investigate the underlying mechanisms of CD20 depletion in the treatment of autoimmune inflammatory disorders, showing that CD20+ B cell depletion alters T cell homing.

Thu May 01 00:00:00 EDT 2014

Regulatory T cells exhibit decreased proliferation but enhanced suppression after pulsing with sirolimus.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Singh K,Kozyr N,Stempora L,Kirk AD,Larsen CP,Blazar BR,Kean LS

14-1278 was used in Flow cytometry/Cell sorting to investigate methods to use Tregs to suppress allo-immunity.

Fri Jun 01 00:00:00 EDT 2012

Significant mobilization of both conventional and regulatory T cells with AMD3100.

Blood

Kean LS,Sen S,Onabajo O,Singh K,Robertson J,Stempora L,Bonifacino AC,Metzger ME,Promislow DE,Mattapallil JJ,Donahue RE

14-1278 was used in Flow cytometry/Cell sorting to indicate that AMD3100 may mobilize a GVHD-protective T-cell repertoire.

Thu Dec 15 00:00:00 EST 2011

CD28 costimulation Impairs the efficacy of a redirected t-cell antitumor attack in the presence of regulatory t cells which can be overcome by preventing Lck activation.

Molecular therapy : the journal of the American Society of Gene Therapy

Kofler DM,Chmielewski M,Rappl G,Hombach A,Riet T,Schmidt A,Hombach AA,Wendtner CM,Abken H

14-1278 was used in Immunohistochemistry on frozen tissues to investigate a novel method which may expedite the implementation of adoptive T-cell therapy in cancer patients.

Fri Apr 01 00:00:00 EDT 2011

Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Nagata S,Ise T,Pastan I

Published figure using CD127 monoclonal antibody (Product # 14-1278-82) in Flow Cytometry

Mon Jun 15 00:00:00 EDT 2009

IL-7 enhances thymic human T cell development in "human immune system" Rag2-/-IL-2Rgammac-/- mice without affecting peripheral T cell homeostasis.

Journal of immunology (Baltimore, Md. : 1950)

van Lent AU,Dontje W,Nagasawa M,Siamari R,Bakker AQ,Pouw SM,Maijoor KA,Weijer K,Cornelissen JJ,Blom B,Di Santo JP,Spits H,Legrand N

14-1278 was used in Flow cytometry/Cell sorting to investigate the role of human IL-7 in newborn BALB/c Rag2(-/-) γc(-/-) mice transplanted with human hematopoietic stem cells as an in vivo model of human hematopoiesis.

Tue Dec 15 00:00:00 EST 2009

Loss of IL-7 receptor alpha on CD4+ T cells defines terminally differentiated B cell-helping effector T cells in a B cell-rich lymphoid tissue.

Journal of immunology (Baltimore, Md. : 1950)

Lim HW,Kim CH

Sat Dec 01 00:00:00 EST 2007

Loss of IL-7 receptor alpha on CD4+ T cells defines terminally differentiated B cell-helping effector T cells in a B cell-rich lymphoid tissue.

Journal of immunology (Baltimore, Md. : 1950)

Lim HW,Kim CH

Sat Dec 01 00:00:00 EST 2007

Bioinformatics

Protein Aliases: CD127; CD127 antigen; CDw127; IL 7 receptor; IL 7R a; IL 7R subunit alpha; IL 7R α; IL 7Ra; IL 7Rα; IL-7 receptor subunit alpha; IL-7R; IL-7R subunit alpha; IL7 receptor; IL7 receptor subunit alpha; IL7R a; IL7R alpha; IL7R subunit alpha; IL7R α; IL7Rα; interleukin 7 receptor alpha chain; interleukin 7 receptor isoform H5-6; Interleukin-7 receptor subunit alpha; Interleukin7 receptor subunit alpha; MGC107557; sCD127; soluble IL 7 receptor; soluble IL 7R

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Gene Aliases: CD127; CDW127; IL-7R-alpha; IL7R; IL7RA; ILRA

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UniProt ID: (Human) P16871

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Entrez Gene ID: (Human) 3575

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Function(s)

antigen binding interleukin-7 receptor activity protein binding transmembrane signal receptor

Process(es)

regulation of DNA recombination cell morphogenesis negative regulation of T cell mediated cytotoxicity immunoglobulin production immune response signal transduction cell surface receptor signaling pathway regulation of cell size positive regulation of gene expression cell growth T cell differentiation positive regulation of T cell differentiation in thymus interleukin-7-mediated signaling pathway B cell proliferation lymph node development homeostasis of number of cells

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CD127 Monoclonal Antibody (eBioRDR5), eBioscience™

CD127 Antibody (14-1278-82) in IHC

Product Details

14-1278-82

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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