Invitrogen

CD127 Monoclonal Antibody (eBioRDR5), APC, eBioscience™

FIGURE: 1 / 1

CD127 Antibody (17-1278-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD3 FITC (Product # 11-0038-42) and Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (left) or Anti-Human CD127 APC (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

Product Details

17-1278-42

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

View 10 publications 10 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioRDR5

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1659670

Product Specific Information

Description: The eBioRDR5 monoclonal antibody reacts with human CD127 (Interleukin-7 Receptor alpha). CD127 complexes with CD132, also known as the common gamma chain (gamma c), to form the multi-functional IL-7 receptor (IL-7R). CD127 is a type I glycoprotein with a molecular weight of 75-80 kDa and is expressed by immature B cells through the early pre-B stage, by thymocytes during several stages of their development, and on most mature T cells, with transient down-regulation upon activation. Binding of IL-7 results in signal transduction which occurs through several tyrosine kinase pathways including the Jak/STAT pathway. IL-7 is indispensible for the development of lymphocytes, and the control of homeostatic proliferation of T-cells in the periphery. In addition, IL-7R signaling is know to be involved in the regulation of T cell receptor (TCR) locus rearrangement in gamma delta T cells.

Interestingly, recently it has been demonstrated that CD127 expression is down-regulated on CD4+CD25+ regulatory T cells (T regs). While the co-expression of CD4 and CD25 has become widely used as an indicator of T regs, this method of identification may also include cells without suppressive activity. It has clearly been shown that CD4+CD25+ cells that have down-regulated the expression of CD127 are significantly more highly-enriched for the regulatory T population, as defined by expression of the T reg-specific transcription factor Foxp3 and the suppressive activity of these cells, in vitro.

Binding of the eBioRDR5 monoclonal antibody to PBMCs is blocked by pre-incubation of the cells with recombinant human IL-7 (Product # 14-1079-80).

Applications Reported: This eBioRDR5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioRDR5 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD127 (Interleukin-7, IL-7) is a glycoprotein involved in the regulation of lymphopoiesis. The response of cells to CD127 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer. CD127 consists of an alpha chain and a gamma chain. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules. CD127 promotes the proliferation of precursor B cells, thymocytes, T cell progenitors, and mature CD4+ and CD8+ T cells. The biological effects of IL7 are mediated by the binding of IL7 to the specific cell surface receptor. Diseases associated with CD127 dysfunction include severe combined immunodeficiency and T cell negative/B cell negative/NK positive severe combined immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Insufficient Iron Improves Pristane-Induced Lupus by Promoting Treg Cell Expansion.

Frontiers in immunology

Gao X,Song Y,Lu S,Hu L,Zheng M,Jia S,Zhao M

17-1278-42 was used in Flow cytometry/Cell sorting to study the role of iron deficiency in the promotion of Treg cell expansion, by reducing ROS accumulation, to improve the disease progression of pristane-induced lupus.

Mon May 02 00:00:00 EDT 2022

Murlentamab, a Low Fucosylated Anti-Müllerian Hormone Type II Receptor (AMHRII) Antibody, Exhibits Anti-Tumor Activity through Tumor-Associated Macrophage Reprogrammation and T Cell Activation.

Cancers

Prat M,Salon M,Allain T,Dubreuil O,Noël G,Preisser L,Jean B,Cassard L,Lemée F,Tabah-Fish I,Pipy B,Jeannin P,Prost JF,Barret JM,Coste A

17-1278 was used in Flow cytometry/Cell sorting to demonstrate here that the murlentamab opsonization of AMHRII-expressing ovarian tumor cells, in the presence of unstimulated- or tumor-associated macrophage (TAM)-like macrophages, significantly promotes macrophage-mediated ADCC and shifts the whole microenvironment towards a pro-inflammatory and anti-tumoral status, thus triggering anti-tumor activity.

Tue Apr 13 00:00:00 EDT 2021

Directed differentiation of regulatory T cells from naive T cells and prevention of their inflammation-mediated instability using small molecules.

Clinical and experimental immunology

Haddadi MH,Negahdari B,Hajizadeh-Saffar E,Khosravi-Maharlooei M,Basiri M,Dabiri H,Baharvand H

17-1278-42 was used in Flow Cytometry to suggest that the N-Ac/SR method has several advantages for Treg generation when compared with the other methods, including a higher purity of Tregs , easier procedure, superior suppressive activity during the inflammatory condition and decreased cost.

Sat Aug 01 00:00:00 EDT 2020

Th2 Biased Immunity With Altered B Cell Profiles in Circulation of Patients With Sporotrichosis Caused by Sporothrix globosa.

Frontiers in immunology

Zu J,Yao L,Song Y,Cui Y,Guan M,Chen R,Zhen Y,Li S

17-1278 was used in Flow cytometry/Cell sorting to shed light on the potential involvement of peripheral T and B cell immunity against this mycotic infection.

Mon May 31 00:00:00 EDT 2021

Hepatocyte-derived exosomes promote T follicular regulatory cell expansion during hepatitis C virus infection.

Hepatology (Baltimore, Md.)

Cobb DA,Kim OK,Golden-Mason L,Rosen HR,Hahn YS

17-1278 was used in Flow cytometry/Cell sorting to examine the accumulation of Tfr cells in the liver compartment during chronic HCV infection and defined the cellular and molecular mechanisms underlying their expansion.

Mon Jan 01 00:00:00 EST 2018

The Effects of High Mobility Group Box-1 Protein on Peripheral Treg/Th17 Balance in Patients with Atherosclerosis.

Acta Cardiologica Sinica

Ding JW,Zhou T,Zheng XX,Wang XA,Tong XH,Luo CY,Zhang ZQ,Yu B

17-1278 was used in Flow cytometry/Cell sorting to explore the mechanism of regulatory T cell/Th17 imbalance and the role of high mobility group box-1 protein on the balance in atherosclerosis.

Sat Sep 01 00:00:00 EDT 2018

Oncogenic IL7R is downregulated by histone deacetylase inhibitor in esophageal squamous cell carcinoma via modulation of acetylated FOXO1.

International journal of oncology

Kim MJ,Choi SK,Hong SH,Eun JW,Nam SW,Han JW,You JS

17-1278-42 was used in Flow cytometry/Cell sorting to demonstrate that targeting oncogenic IL7R in ESCC by HDAC inhibitors may be a valuable therapeutic approach.

Sun Jul 01 00:00:00 EDT 2018

Regulatory T cells produce profibrotic cytokines in the skin of patients with systemic sclerosis.

The Journal of allergy and clinical immunology

MacDonald KG,Dawson NAJ,Huang Q,Dunne JV,Levings MK,Broady R

17-1278 was used in Flow cytometry/Cell sorting to determine whether Treg cells in the blood and skin of patients with SSc acquired abnormal production of effector cytokines.

Wed Apr 01 00:00:00 EDT 2015

The APPEESFRS Peptide, Restricted by the HLA-B*35:01 Molecule, and the APPEESFRF Variant Derived from an Autologous HIV-1 Strain Induces Polyfunctional Responses in CD8+ T Cells.

BioResearch open access

Acevedo-Sáenz L,Carmona-Pérez L,Velilla-Hernández PA,Delgado JC,Rugeles L MT

17-1278 was used in Flow cytometry/Cell sorting to determine the effector profile and maturation phenotype of CD8+ T-cells targeting the consensus APPEESFRS (AS9) epitope and its variant APPEESFRF (AF9).

Fri Aug 28 00:00:00 EDT 2015

Loss of IL-7 receptor alpha on CD4+ T cells defines terminally differentiated B cell-helping effector T cells in a B cell-rich lymphoid tissue.

Journal of immunology (Baltimore, Md. : 1950)

Lim HW,Kim CH

Sat Dec 01 00:00:00 EST 2007

Loss of IL-7 receptor alpha on CD4+ T cells defines terminally differentiated B cell-helping effector T cells in a B cell-rich lymphoid tissue.

Journal of immunology (Baltimore, Md. : 1950)

Lim HW,Kim CH

Sat Dec 01 00:00:00 EST 2007

Bioinformatics

Protein Aliases: CD127; CD127 antigen; CDw127; IL 7 receptor; IL 7R a; IL 7R subunit alpha; IL 7R α; IL 7Ra; IL 7Rα; IL-7 receptor subunit alpha; IL-7R; IL-7R subunit alpha; IL7 receptor; IL7 receptor subunit alpha; IL7R a; IL7R alpha; IL7R subunit alpha; IL7R α; IL7Rα; interleukin 7 receptor alpha chain; interleukin 7 receptor isoform H5-6; Interleukin-7 receptor subunit alpha; Interleukin7 receptor subunit alpha; MGC107557; sCD127; soluble IL 7 receptor; soluble IL 7R

View more View less

Gene Aliases: CD127; CDW127; IL-7R-alpha; IL7R; IL7RA; ILRA

View more View less

UniProt ID: (Human) P16871

View more View less

Entrez Gene ID: (Human) 3575

View more View less

Function(s)

antigen binding interleukin-7 receptor activity protein binding transmembrane signal receptor

Process(es)

regulation of DNA recombination cell morphogenesis negative regulation of T cell mediated cytotoxicity immunoglobulin production immune response signal transduction cell surface receptor signaling pathway regulation of cell size positive regulation of gene expression cell growth T cell differentiation positive regulation of T cell differentiation in thymus interleukin-7-mediated signaling pathway B cell proliferation lymph node development homeostasis of number of cells

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD127 Monoclonal Antibody (eBioRDR5), APC, eBioscience™

CD127 Antibody (17-1278-42) in Flow

Product Details

17-1278-42

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!