Invitrogen

CD8a Monoclonal Antibody (RPA-T8), PE-Cyanine5, eBioscience™

FIGURE: 1 / 41

CD8a Antibody (15-0088-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG1 isotype control PE-Cyanine5 (Product # 15-4714-81) (open histogram) or Anti-Human CD8a PE-Cyanine5 (filled histogram).Cells in the lymphocyte gate were used for analysis.

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Product Details

15-0088-42

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 31 publications 31 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Rat

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine5, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

RPA-T8

Conjugate

PE-Cyanine5 PE-Cyanine5 PE-Cyanine5

Excitation/Emission Max

568/666 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1633398

Product Specific Information

Description: The RPA-T8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells.

Applications Reported: The RPA-T8 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This RPA-T8 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Severe T cell hyporeactivity in ventilated COVID-19 patients correlates with prolonged virus persistence and poor outcomes.

Nature communications

Renner K,Schwittay T,Chaabane S,Gottschling J,Müller C,Tiefenböck C,Salewski JN,Winter F,Buchtler S,Balam S,Malfertheiner MV,Lubnow M,Lunz D,Graf B,Hitzenbichler F,Hanses F,Poeck H,Kreutz M,Orsó E,Burkhardt R,Niedermair T,Brochhausen C,Gessner A,Salzberger B,Mack M

Published figure using CD8a monoclonal antibody (Product # 15-0088-42) in Flow Cytometry

Fri May 21 00:00:00 EDT 2021

Multipeptide stimulated PBMCs generate T_EM/T_CM for adoptive cell therapy in multiple myeloma.

Oncotarget

Vardam-Kaur T,Pathangey LB,McCormick DJ,Bergsagel PL,Cohen PA,Gendler SJ

Published figure using CD8a monoclonal antibody (Product # 15-0088-42) in Flow Cytometry

Tue Sep 28 00:00:00 EDT 2021

Human endoglin-CD3 bispecific T cell engager antibody induces anti-tumor effect in vivo.

Theranostics

Zhong L,Shi W,Gan L,Liu X,Huo Y,Wu P,Zhang Z,Wu T,Peng H,Huang Y,Zhao Y,Yuan Y,Deng Z,Tang H

Published figure using CD8a monoclonal antibody (Product # 15-0088-42) in Flow Cytometry

Wed Jul 28 00:00:00 EDT 2021

Bispecific T-Cell Engaging Antibodies Against MUC16 Demonstrate Efficacy Against Ovarian Cancer in Monotherapy and in Combination With PD-1 and VEGF Inhibition.

Frontiers in immunology

Yeku OO,Rao TD,Laster I,Kononenko A,Purdon TJ,Wang P,Cui Z,Liu H,Brentjens RJ,Spriggs D

Published figure using CD8a monoclonal antibody (Product # 15-0088-42) in Flow Cytometry

Thu Oct 21 00:00:00 EDT 2021

Activation of Bone Marrow Adaptive Immunity in Type 2 Diabetes: Rescue by Co-stimulation Modulator Abatacept.

Frontiers in immunology

Santopaolo M,Sullivan N,Thomas AC,Alvino VV,Nicholson LB,Gu Y,Spinetti G,Kallikourdis M,Blom A,Madeddu P

15-0088-42 was used in Flow Cytometry to identify the role of Abatacept in reducing the demonstrated T cell activation in the bone marrow of type 2 diabetes patients and mice.

Fri Jul 02 00:00:00 EDT 2021

Preventing ATP Degradation by ASO-Mediated Knockdown of CD39 and CD73 Results in A2aR-Independent Rescue of T Cell Proliferation.

Molecular therapy. Nucleic acids

Festag J,Thelemann T,Schell M,Raith S,Michel S,Jaschinski F,Klar R

Published figure using CD8a monoclonal antibody (Product # 15-0088-42) in Flow Cytometry

Fri Sep 04 00:00:00 EDT 2020

A Cross-Reactive Small Protein Binding Domain Provides a Model to Study Off-Tumor CAR-T Cell Toxicity.

Molecular therapy oncolytics

Hammill JA,Kwiecien JM,Dvorkin-Gheva A,Lau VWC,Baker C,Wu Y,Bezverbnaya K,Aarts C,Heslen CW,Denisova GF,Derocher H,Milne K,Nelson BH,Bramson JL

Published figure using CD8a monoclonal antibody (Product # 15-0088-42) in Flow Cytometry

Fri Jun 26 00:00:00 EDT 2020

Immunological Properties of Neural Crest Cells Derived from Human Induced Pluripotent Stem Cells.

Stem cells and development

Fujii S,Yoshida S,Inagaki E,Hatou S,Tsubota K,Takahashi M,Shimmura S,Sugita S

Published figure using CD8a monoclonal antibody (Product # 15-0088-42) in Flow Cytometry

Tue Jan 01 00:00:00 EST 2019

Functionalization-dependent effects of cellulose nanofibrils on tolerogenic mechanisms of human dendritic cells.

International journal of nanomedicine

Tomić S,Ilić N,Kokol V,Gruden-Movsesijan A,Mihajlović D,Bekić M,Sofronić-Milosavljević L,Čolić M,Vučević D

15-0088 was used in Flow cytometry/Cell sorting to investigate how differentially functionalised cellulose nanofibrils affect dendritic cell properties, their viability, morphology, differentiation and maturation potential, and the capacity to regulate T cell-mediated immune response.

Mon Dec 31 00:00:00 EST 2018

The Synergistic Effects of the Glutathione Precursor, NAC and First-Line Antibiotics in the Granulomatous Response Against Mycobacterium tuberculosis.

Frontiers in immunology

Teskey G,Cao R,Islamoglu H,Medina A,Prasad C,Prasad R,Sathananthan A,Fraix M,Subbian S,Zhong L,Venketaraman V

15-0088 was used in Immunocytochemistry-immunoflourescence to examine if N-Acetyl Cysteine in combination with antibiotics would be sufficient to completely clear Mycobacterium tuberculosis infection within in vitro granulomas.

Mon Sep 30 00:00:00 EDT 2019

Blocking the recruitment of naive CD4⁺ T cells reverses immunosuppression in breast cancer.

Cell research

Su S,Liao J,Liu J,Huang D,He C,Chen F,Yang L,Wu W,Chen J,Lin L,Zeng Y,Ouyang N,Cui X,Yao H,Su F,Huang JD,Lieberman J,Liu Q,Song E

Published figure using CD8a monoclonal antibody (Product # 15-0088-42) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

Detection of Retinal Pigment Epithelium-Specific Antibody in iPSC-Derived Retinal Pigment Epithelium Transplantation Models.

Stem cell reports

Sugita S,Makabe K,Fujii S,Iwasaki Y,Kamao H,Shiina T,Ogasawara K,Takahashi M

Published figure using CD8a monoclonal antibody (Product # 15-0088-42) in Flow Cytometry

Tue Nov 14 00:00:00 EST 2017

Cytoplasmic Localization of HTLV-1 HBZ Protein: A Biomarker of HTLV-1-Associated Myelopathy/Tropical Spastic Paraparesis (HAM/TSP).

PLoS neglected tropical diseases

Baratella M,Forlani G,Raval GU,Tedeschi A,Gout O,Gessain A,Tosi G,Accolla RS

15-0088 was used in Flow cytometry/Cell sorting to identify the cytoplasmic localization of HTLV-1 HBZ protein as a possible biomarker for HTLV-1-associated myelopathy/tropical spastic paraparesis.

Sun Jan 01 00:00:00 EST 2017

Follicular Regulatory CD8 T Cells Impair the Germinal Center Response in SIV and Ex Vivo HIV Infection.

PLoS pathogens

Miles B,Miller SM,Folkvord JM,Levy DN,Rakasz EG,Skinner PJ,Connick E

Published figure using CD8a monoclonal antibody (Product # 15-0088-42) in Flow Cytometry

Sat Oct 01 00:00:00 EDT 2016

Human Head and Neck Squamous Cell Carcinoma-Associated Semaphorin 4D Induces Expansion of Myeloid-Derived Suppressor Cells.

Journal of immunology (Baltimore, Md. : 1950)

Younis RH,Han KL,Webb TJ

Published figure using CD8a monoclonal antibody (Product # 15-0088-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2016

Influenza Vaccination Generates Cytokine-Induced Memory-like NK Cells: Impact of Human Cytomegalovirus Infection.

Journal of immunology (Baltimore, Md. : 1950)

Goodier MR,Rodriguez-Galan A,Lusa C,Nielsen CM,Darboe A,Moldoveanu AL,White MJ,Behrens R,Riley EM

15-0088 was used in Flow cytometry/Cell sorting to examine the effect of seasonal influenza vaccination on natural killer cell function and phenotype in previously unvaccinated individuals.

Fri Jul 01 00:00:00 EDT 2016

Umbilical Cord-Derived Mesenchymal Stem Cells Suppress Autophagy of T Cells in Patients with Systemic Lupus Erythematosus via Transfer of Mitochondria.

Stem cells international

Chen J,Wang Q,Feng X,Zhang Z,Geng L,Xu T,Wang D,Sun L

Published figure using CD8a monoclonal antibody (Product # 15-0088-42) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

CD28 family of receptors on T cells in chronic HBV infection: Expression characteristics, clinical significance and correlations with PD-1 blockade.

Molecular medicine reports

Tang ZS,Hao YH,Zhang EJ,Xu CL,Zhou Y,Zheng X,Yang DL

Published figure using CD8a monoclonal antibody (Product # 15-0088-42) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

Regulatory T Cells in Patients with Idiopathic Thrombocytopenic Purpura.

Turkish journal of haematology : official journal of Turkish Society of Haematology

Akyol Erikçi A,Karagöz B,Bilgi O

15-0088 was used in Flow cytometry/Cell sorting to investigate the role of regulatory T Cells in patients with Idiopathic Thrombocytopenic Purpura.

Sun Jun 05 00:00:00 EDT 2016

Lack of T Cell Response to iPSC-Derived Retinal Pigment Epithelial Cells from HLA Homozygous Donors.

Stem cell reports

Sugita S,Iwasaki Y,Makabe K,Kimura T,Futagami T,Suegami S,Takahashi M

Published figure using CD8a monoclonal antibody (Product # 15-0088-42) in Flow Cytometry

Tue Oct 11 00:00:00 EDT 2016

Liposomal Glutathione Supplementation Restores TH1 Cytokine Response to Mycobacterium tuberculosis Infection in HIV-Infected Individuals.

Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research

Ly J,Lagman M,Saing T,Singh MK,Tudela EV,Morris D,Anderson J,Daliva J,Ochoa C,Patel N,Pearce D,Venketaraman V

15-0088 was used in Flow cytometry/Cell sorting to establish a correlation between low levels of glutathione and an increased susceptibility to M. tuberculosis infection.

Sun Nov 01 00:00:00 EDT 2015

Follicular regulatory T cells impair follicular T helper cells in HIV and SIV infection.

Nature communications

Miles B,Miller SM,Folkvord JM,Kimball A,Chamanian M,Meditz AL,Arends T,McCarter MD,Levy DN,Rakasz EG,Skinner PJ,Connick E

Published figure using CD8a monoclonal antibody (Product # 15-0088-42) in Flow Cytometry

Tue Oct 20 00:00:00 EDT 2015

RSV-specific airway resident memory CD8+ T cells and differential disease severity after experimental human infection.

Nature communications

Jozwik A,Habibi MS,Paras A,Zhu J,Guvenel A,Dhariwal J,Almond M,Wong EHC,Sykes A,Maybeno M,Del Rosario J,Trujillo-Torralbo MB,Mallia P,Sidney J,Peters B,Kon OM,Sette A,Johnston SL,Openshaw PJ,Chiu C

Published figure using CD8a monoclonal antibody (Product # 15-0088-42) in Flow Cytometry

Mon Dec 21 00:00:00 EST 2015

Investigating the causes for decreased levels of glutathione in individuals with type II diabetes.

PloS one

Lagman M,Ly J,Saing T,Kaur Singh M,Vera Tudela E,Morris D,Chi PT,Ochoa C,Sathananthan A,Venketaraman V

15-0088 was used in Flow cytometry/Cell sorting to study the mechanisms of reduced levels of glutathione in sufferers of type II diabetes and the effect of this on susceptibility to tuberculosis infection.

Wed Dec 30 00:00:00 EST 2015

Localization, quantification and interaction with host factors of endogenous HTLV-1 HBZ protein in infected cells and ATL.

Retrovirology

Raval GU,Bidoia C,Forlani G,Tosi G,Gessain A,Accolla RS

15-0088 was used in Flow cytometry/Cell sorting to investigate the role of HBZ in oncogenesis.

Sat Jul 04 00:00:00 EDT 2015

Immune response to JC virus T antigen in patients with and without colorectal neoplasia.

Gut microbes

Butcher LD,Garcia M,Arnold M,Ueno H,Goel A,Boland CR

Published figure using CD8a monoclonal antibody (Product # 15-0088-42) in Flow Cytometry

Tue Jul 01 00:00:00 EDT 2014

CD4+CD25(high), CD8+CD28- cells and thyroid autoantibodies in breast cancer patients.

Central-European journal of immunology

Biylgi O,Karagöz B,Türken O,Gültepe M,Özgün A,Tunçel T,Emirzeoğlu L,Çelik S,Müftüoğlu T,Gökhan Kandemir E

15-0088 was used in Flow cytometry/Cell sorting to investigate the percentage of CD4+CD25(high) cells (including Treg cells) and CD8+CD28- cells in breast cancer patients with and without high levels of autoimmune thyroid antibodies.

Thu Jul 09 00:00:00 EDT 2015

Ex vivo analysis of resident hepatic pro-inflammatory CD1d-reactive T cells and hepatocyte surface CD1d expression in hepatitis C.

Journal of viral hepatitis

Yanagisawa K,Yue S,van der Vliet HJ,Wang R,Alatrakchi N,Golden-Mason L,Schuppan D,Koziel MJ,Rosen HR,Exley MA

Published figure using CD8a monoclonal antibody (Product # 15-0088-42) in Flow Cytometry

Thu Aug 01 00:00:00 EDT 2013

Upregulation of programmed death-1 on T cells and programmed death ligand-1 on monocytes in septic shock patients.

Critical care (London, England)

Zhang Y,Li J,Lou J,Zhou Y,Bo L,Zhu J,Zhu K,Wan X,Cai Z,Deng X

Published figure using CD8a monoclonal antibody (Product # 15-0088-42) in Flow Cytometry

Wed Dec 07 00:00:00 EST 2011

Sepsis-induced human lymphocyte apoptosis and cytokine production in "humanized" mice.

Journal of leukocyte biology

Unsinger J,McDonough JS,Shultz LD,Ferguson TA,Hotchkiss RS

15-0088 was used in Flow cytometry/Cell sorting to study sepsis-induced human lymphocyte apoptosis and cytokine production in "humanized" mice.

Sat Aug 01 00:00:00 EDT 2009

B cells and monocytes from patients with active multiple sclerosis exhibit increased surface expression of both HERV-H Env and HERV-W Env, accompanied by increased seroreactivity.

Retrovirology

Brudek T,Christensen T,Aagaard L,Petersen T,Hansen HJ,Møller-Larsen A

Published figure using CD8a monoclonal antibody (Product # 15-0088-42) in Flow Cytometry

Mon Nov 16 00:00:00 EST 2009

Toll-like receptors in cellular subsets of human tonsil T cells: altered expression during recurrent tonsillitis.

Respiratory research

Mansson A,Adner M,Cardell LO

15-0088 was used in Flow cytometry/Cell sorting to investigate the role of toll-like receptors in human tonsil CD4+ and CD8+ T cells, and their altered expression in patients with recurrent tonsillitis.

Mon Feb 27 00:00:00 EST 2006

Bioinformatics

Protein Aliases: CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Leu2 T-lymphocyte antigen; OKT8 T-cell antigen; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen

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Gene Aliases: CD8; CD8A; CD8B; CD8B1; LEU2; LY3; LYT3; MAL; p32; P37

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UniProt ID: (Human) P01732, (Human) P10966

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Entrez Gene ID: (Human) 925, (Human) 926

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Function(s)

protein binding coreceptor activity protein kinase binding MHC class I protein binding protein homodimerization activity defense/immunity protein immunoglobulin receptor superfamily

Process(es)

T cell mediated immunity immune response cell surface receptor signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway antigen processing and presentation T cell activation cytotoxic T cell differentiation regulation of immune response positive regulation of calcium-mediated signaling defense response to virus adaptive immune response regulation of defense response to virus by virus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD8a Monoclonal Antibody (RPA-T8), PE-Cyanine5, eBioscience™

CD8a Antibody (15-0088-42) in Flow

Product Details

15-0088-42

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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