Invitrogen

CD45.1 Monoclonal Antibody (A20), PE-Cyanine5, eBioscience™

CD45.1 Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 26

CD45.1 Antibody (15-0453-82) in Flow

Staining of SJL splenocytes with staining buffer (autofluorescence) (open histogram) or 0.25 µg Anti-Mouse CD45-1 PE-Cyanine5 (filled histogram).Total cells were used for analysis.

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Product Details

15-0453-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 32 publications 32 publications

Product Specifications

Species Reactivity

Mouse

Published species

Fish, Mouse

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), PE-Cyanine5, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

A20

Conjugate

PE-Cyanine5 PE-Cyanine5 PE-Cyanine5

Excitation/Emission Max

568/666 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468759

Product Specific Information

Description: The A20 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.1-expressing mouse strains. The strains that express CD45.1 include SJL/J, DA, STS/A and RIII. CD45.1 is expressed by all leukocytes in these strains.

Applications Reported: The A20 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The A20 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Dlk1 maintains adult mice long-term HSCs by activating Notch signaling to restrict mitochondrial metabolism.

Experimental hematology & oncology

Huang D,Han Y,Tang T,Yang L,Jiang P,Qian W,Zhang Z,Qian X,Zeng X,Qian P

15-0453-82 was used in Flow cytometry/Cell sorting to demonstrate that Dlk1 is essential for maintaining HSC homeostasis, which is realized by governing Notch signalling and restricting mitochondrial metabolic activity.

Wed Jan 18 00:00:00 EST 2023

Tet-mediated DNA demethylation regulates specification of hematopoietic stem and progenitor cells during mammalian embryogenesis.

Science advances

Ma L,Tang Q,Gao X,Lee J,Lei R,Suzuki M,Zheng D,Ito K,Frenette PS,Dawlaty MM

Published figure using CD45.1 monoclonal antibody (Product # 15-0453-82) in Flow Cytometry

Fri Mar 04 00:00:00 EST 2022

Smc3 dosage regulates B cell transit through germinal centers and restricts their malignant transformation.

Nature immunology

Rivas MA,Meydan C,Chin CR,Challman MF,Kim D,Bhinder B,Kloetgen A,Viny AD,Teater MR,McNally DR,Doane AS,Béguelin W,Fernández MTC,Shen H,Wang X,Levine RL,Chen Z,Tsirigos A,Elemento O,Mason CE,Melnick AM

Published figure using CD45.1 monoclonal antibody (Product # 15-0453-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Aged hematopoietic stem cells are refractory to bloodborne systemic rejuvenation interventions.

The Journal of experimental medicine

Ho TT,Dellorusso PV,Verovskaya EV,Bakker ST,Flach J,Smith LK,Ventura PB,Lansinger OM,Hérault A,Zhang SY,Kang YA,Mitchell CA,Villeda SA,Passegué E

Published figure using CD45.1 monoclonal antibody (Product # 15-0453-82) in Flow Cytometry

Mon Jul 05 00:00:00 EDT 2021

Differentiation of fetal hematopoietic stem cells requires ARID4B to restrict autocrine KITLG/KIT-Src signaling.

Cell reports

Young IC,Wu B,Andricovich J,Chuang ST,Li R,Tzatsos A,Wu RC,Wu MY

Published figure using CD45.1 monoclonal antibody (Product # 15-0453-82) in Flow Cytometry

Tue Nov 23 00:00:00 EST 2021

Development of a VRC01-class germline targeting immunogen derived from anti-idiotypic antibodies.

Cell reports

Seydoux E,Wan YH,Feng J,Wall A,Aljedani S,Homad LJ,MacCamy AJ,Weidle C,Gray MD,Brumage L,Taylor JJ,Pancera M,Stamatatos L,McGuire AT

Published figure using CD45.1 monoclonal antibody (Product # 15-0453-82) in Flow Cytometry

Tue May 04 00:00:00 EDT 2021

Ddb1 Is Essential for the Expansion of CD4⁺ Helper T Cells by Regulating Cell Cycle Progression and Cell Death.

Frontiers in immunology

Yang L,Chen W,Li L,Xiao Y,Fan S,Zhang Q,Xia T,Li M,Hong Y,Zhao T,Li Q,Liu WH,Xiao N

Published figure using CD45.1 monoclonal antibody (Product # 15-0453-82) in Flow Cytometry

Mon Dec 20 00:00:00 EST 2021

Deregulated Notch and Wnt signaling activates early-stage myeloid regeneration pathways in leukemia.

The Journal of experimental medicine

Kang YA,Pietras EM,Passegué E

Published figure using CD45.1 monoclonal antibody (Product # 15-0453-82) in Flow Cytometry

Mon Mar 02 00:00:00 EST 2020

RIPK3 and Caspase-1/11 Are Necessary for Optimal Antigen-Specific CD8 T Cell Response Elicited by Genetically Modified Listeria monocytogenes.

Frontiers in immunology

Rana A,de Almeida FC,Paico Montero HA,Gonzales Carazas MM,Bortoluci KR,Sad S,Amarante-Mendes GP

Published figure using CD45.1 monoclonal antibody (Product # 15-0453-82) in Flow Cytometry

Fri Mar 26 00:00:00 EDT 2021

Engineering monocyte/macrophage-specific glucocerebrosidase expression in human hematopoietic stem cells using genome editing.

Nature communications

Scharenberg SG,Poletto E,Lucot KL,Colella P,Sheikali A,Montine TJ,Porteus MH,Gomez-Ospina N

Published figure using CD45.1 monoclonal antibody (Product # 15-0453-82) in Flow Cytometry

Fri Jul 03 00:00:00 EDT 2020

Neurokinin-1 Receptor Signaling Is Required for Efficient Ca²⁺ Flux in T-Cell-Receptor-Activated T Cells.

Cell reports

Morelli AE,Sumpter TL,Rojas-Canales DM,Bandyopadhyay M,Chen Z,Tkacheva O,Shufesky WJ,Wallace CT,Watkins SC,Berger A,Paige CJ,Falo LD,Larregina AT

15-0453 was used in Flow cytometry/Cell sorting to demonstrate an effect of the NK1R in T cells that is relevant for immunotherapies based on pro-inflammatory neuropeptides and its receptors.

Tue Mar 10 00:00:00 EDT 2020

N-Cadherin-Expressing Bone and Marrow Stromal Progenitor Cells Maintain Reserve Hematopoietic Stem Cells.

Cell reports

Zhao M,Tao F,Venkatraman A,Li Z,Smith SE,Unruh J,Chen S,Ward C,Qian P,Perry JM,Marshall H,Wang J,He XC,Li L

15-0453 was used in Flow cytometry/Cell sorting to report that N-cad+ cells were functional bone and marrow stromal progenitor cells, giving rise to osteoblasts, adipocytes, and chondrocytes both in vitro and in vivo.

Tue Jan 15 00:00:00 EST 2019

Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells.

Cell

Gaya M,Barral P,Burbage M,Aggarwal S,Montaner B,Warren Navia A,Aid M,Tsui C,Maldonado P,Nair U,Ghneim K,Fallon PG,Sekaly RP,Barouch DH,Shalek AK,Bruckbauer A,Strid J,Batista FD

Published figure using CD45.1 monoclonal antibody (Product # 15-0453-82) in Flow Cytometry

Thu Jan 25 00:00:00 EST 2018

CRISPR/Cas9 genome editing in human hematopoietic stem cells.

Nature protocols

Bak RO,Dever DP,Porteus MH

15-0453 was used in Flow cytometry/Cell sorting to contribute to a protocol for the production, enrichment, and in vitro and in vivo analyses of HR-targeted hematopoietic stem cells.

Thu Feb 01 00:00:00 EST 2018

The H3K4 methyltransferase Setd1b is essential for hematopoietic stem and progenitor cell homeostasis in mice.

eLife

Schmidt K,Zhang Q,Tasdogan A,Petzold A,Dahl A,Arneth BM,Slany R,Fehling HJ,Kranz A,Stewart AF,Anastassiadis K

Published figure using CD45.1 monoclonal antibody (Product # 15-0453-82) in Flow Cytometry

Tue Jun 19 00:00:00 EDT 2018

An immune cell spray (ICS) formulation allows for the delivery of functional monocyte/macrophages.

Scientific reports

Beneke V,Küster F,Neehus AL,Hesse C,Lopez-Rodriguez E,Haake K,Rafiei Hashtchin A,Schott JW,Walter D,Braun A,Wolkers WF,Ackermann M,Lachmann N

Published figure using CD45.1 monoclonal antibody (Product # 15-0453-82) in Flow Cytometry

Fri Nov 02 00:00:00 EDT 2018

Retinoid-Sensitive Epigenetic Regulation of the Hoxb Cluster Maintains Normal Hematopoiesis and Inhibits Leukemogenesis.

Cell stem cell

Qian P,De Kumar B,He XC,Nolte C,Gogol M,Ahn Y,Chen S,Li Z,Xu H,Perry JM,Hu D,Tao F,Zhao M,Han Y,Hall K,Peak A,Paulson A,Zhao C,Venkatraman A,Box A,Perera A,Haug JS,Parmely T,Li H,Krumlauf R,Li L

15-0453 was used in Flow cytometry/Cell sorting to demonstrate roles for retinoid signaling and the DERARE in maintaining HSCs and preventing leukemogenesis by coordinate regulation of Hoxb genes.

Thu May 03 00:00:00 EDT 2018

Egr2 and 3 control adaptive immune responses by temporally uncoupling expansion from T cell differentiation.

The Journal of experimental medicine

Miao T,Symonds ALJ,Singh R,Symonds JD,Ogbe A,Omodho B,Zhu B,Li S,Wang P

Published figure using CD45.1 monoclonal antibody (Product # 15-0453-82) in Flow Cytometry

Mon Jun 05 00:00:00 EDT 2017

The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity.

Cell

Damgaard RB,Walker JA,Marco-Casanova P,Morgan NV,Titheradge HL,Elliott PR,McHale D,Maher ER,McKenzie ANJ,Komander D

Published figure using CD45.1 monoclonal antibody (Product # 15-0453-82) in Flow Cytometry

Thu Aug 25 00:00:00 EDT 2016

Murine iPSC-Derived Macrophages as a Tool for Disease Modeling of Hereditary Pulmonary Alveolar Proteinosis due to Csf2rb Deficiency.

Stem cell reports

Mucci A,Kunkiel J,Suzuki T,Brennig S,Glage S,Kühnel MP,Ackermann M,Happle C,Kuhn A,Schambach A,Trapnell BC,Hansen G,Moritz T,Lachmann N

Published figure using CD45.1 monoclonal antibody (Product # 15-0453-82) in Flow Cytometry

Tue Aug 09 00:00:00 EDT 2016

MHC-mismatched mixed chimerism augments thymic regulatory T-cell production and prevents relapse of EAE in mice.

Proceedings of the National Academy of Sciences of the United States of America

Wu L,Li N,Zhang M,Xue SL,Cassady K,Lin Q,Riggs AD,Zeng D

Published figure using CD45.1 monoclonal antibody (Product # 15-0453-82) in Flow Cytometry

Tue Dec 29 00:00:00 EST 2015

Alternatively activated macrophages promote pancreatic fibrosis in chronic pancreatitis.

Nature communications

Xue J,Sharma V,Hsieh MH,Chawla A,Murali R,Pandol SJ,Habtezion A

Published figure using CD45.1 monoclonal antibody (Product # 15-0453-82) in Flow Cytometry

Mon May 18 00:00:00 EDT 2015

Heme-mediated SPI-C induction promotes monocyte differentiation into iron-recycling macrophages.

Cell

Haldar M,Kohyama M,So AY,Kc W,Wu X,Briseño CG,Satpathy AT,Kretzer NM,Arase H,Rajasekaran NS,Wang L,Egawa T,Igarashi K,Baltimore D,Murphy TL,Murphy KM

Published figure using CD45.1 monoclonal antibody (Product # 15-0453-82) in Flow Cytometry

Thu Mar 13 00:00:00 EDT 2014

WASH is required for the differentiation commitment of hematopoietic stem cells in a c-Myc-dependent manner.

The Journal of experimental medicine

Xia P,Wang S,Huang G,Zhu P,Li M,Ye B,Du Y,Fan Z

Published figure using CD45.1 monoclonal antibody (Product # 15-0453-82) in Flow Cytometry

Mon Sep 22 00:00:00 EDT 2014

Mutual expression of the transcription factors Runx3 and ThPOK regulates intestinal CD4⁺ T cell immunity.

Nature immunology

Reis BS,Rogoz A,Costa-Pinto FA,Taniuchi I,Mucida D

Published figure using CD45.1 monoclonal antibody (Product # 15-0453-82) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2013

Arrayed lentiviral barcoding for quantification analysis of hematopoietic dynamics.

Stem cells (Dayton, Ohio)

Grosselin J,Sii-Felice K,Payen E,Chretien S,Tronik-Le Roux D,Leboulch P

Published figure using CD45.1 monoclonal antibody (Product # 15-0453-82) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2013

In vitro Ag Cross-presentation and in vivo Ag Cross-presentation by Dendritic Cells in the Mouse.

Bio-protocol

Ghosh M,Shapiro LH

Published figure using CD45.1 monoclonal antibody (Product # 15-0453-82) in Flow Cytometry

Thu Dec 20 00:00:00 EST 2012

Regulatory T cells modulate postischemic neovascularization.

Circulation

Zouggari Y,Ait-Oufella H,Waeckel L,Vilar J,Loinard C,Cochain C,Récalde A,Duriez M,Levy BI,Lutgens E,Mallat Z,Silvestre JS

15-0453 was used in Flow cytometry/Cell sorting to study the role of Treg cell activation in postischemic response and vessel growth.

Tue Oct 06 00:00:00 EDT 2009

Viral sequestration of antigen subverts cross presentation to CD8(+) T cells.

PLoS pathogens

Tewalt EF,Grant JM,Granger EL,Palmer DC,Heuss ND,Gregerson DS,Restifo NP,Norbury CC

15-0453 was used in Flow cytometry/Cell sorting to describe an evasion mechanism employed by pathogens to prevent entry into the cross-presentation pathway.

Fri May 01 00:00:00 EDT 2009

Allogeneic stromal cell implantation in brain tissue leads to robust microglial activation.

Immunology and cell biology

Tambuyzer BR,Bergwerf I,De Vocht N,Reekmans K,Daans J,Jorens PG,Goossens H,Ysebaert DK,Chatterjee S,Van Marck E,Berneman ZN,Ponsaerts P

15-0453 was used in Flow cytometry/Cell sorting to describe the immunological mechanism leading to rejection of allogeneic BM-SCs after implantation in murine CNS.

Fri Jun 19 00:00:00 EDT 2009

Dynamic imaging of T cell-parasite interactions in the brains of mice chronically infected with Toxoplasma gondii.

Journal of immunology (Baltimore, Md. : 1950)

Schaeffer M,Han SJ,Chtanova T,van Dooren GG,Herzmark P,Chen Y,Roysam B,Striepen B,Robey EA

15-0453 was used in Flow cytometry/Cell sorting to directly observe the dynamics of T cell-parasite interactions within living tissue and further understand immune responses to persistent pathogens in the brain.

Fri May 15 00:00:00 EDT 2009

Maintenance of the hematopoietic stem cell pool by CXCL12-CXCR4 chemokine signaling in bone marrow stromal cell niches.

Immunity

Sugiyama T,Kohara H,Noda M,Nagasawa T

15-0453 was used in Flow cytometry/Cell sorting to show that induced deletion of CXCR4 in adult mice leads to severe reduction of haematopoietic stem cells and increased sensitivity to myelotoxic injury.

Fri Dec 01 00:00:00 EST 2006

Bioinformatics

Protein Aliases: CD45; CD45 antigen; GP180; L-CA; Leukocyte common antigen; Ly-5; LY5; Lymphocyte antigen 5; lymphocyte common antigen; Receptor-type tyrosine-protein phosphatase C; RT7; T200; T200 glycoprotein

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Gene Aliases: B220; Cd45; CD45R; L-CA; loc; Ly-5; Lyt-4; Ptprc; T200

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UniProt ID: (Mouse) P06800

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Entrez Gene ID: (Mouse) 19264

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Function(s)

phosphoprotein phosphatase activity protein tyrosine phosphatase activity protein binding heparin binding hydrolase activity phosphatase activity protein kinase binding heparan sulfate proteoglycan binding protein phosphatase protein modifying enzyme

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD45.1 Monoclonal Antibody (A20), PE-Cyanine5, eBioscience™

CD45.1 Antibody (15-0453-82) in Flow

Product Details

15-0453-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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