Invitrogen

T-bet Monoclonal Antibody (eBio4B10 (4B10)), PE-Cyanine5, eBioscience™

20 Published Figures

20 References

View all (31) T-bet antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (20)

FIGURE: 1 / 21

T-bet Antibody (15-5825-82) in Flow

Normal human peripheral blood cells were stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol, with CD8a Monoclonal Antibody, eFluor 450 (Product # 48-0087-42) and 0.125 µg of Mouse IgG1 kappa Isotype Control, PE-Cyanine5 (Product # 15-4714-81) (left) or 0.125 µg of T-bet Monoclonal Antibody, PE-Cyanine5 (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

15-5825-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 20 publications 20 publications

Product Specifications

Species Reactivity

Human, Mouse, Rhesus monkey

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine5, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBio4B10 (4B10)

Conjugate

PE-Cyanine5 PE-Cyanine5 PE-Cyanine5

Excitation/Emission Max

568/666 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2815071

Product Specific Information

Description: The eBio4B10 monoclonal antibody reacts with mouse and human T-bet. T-bet is a Th1-specific T-box transcription factor critical to the development of the Th1 CD4+ T cell lineage. This is known based on the observations that T-Bet deficient mice have impaired Th1 cell development, and that ectopic expression of T-Bet results in development skewed to the Th1 lineage. T-Bet expression is induced by the Th1 cytokine IFN gamma, and T-Bet also regulates the expression of IFN gamma, likely, at least in part, through the modification of DNA accessibility and histone remodeling. In addition to IFN gamma, T-Bet is also known to regulate the expression of IL-12R beta and IL-2. Moreover, T-Bet plays a role in class-switch recombination in B-cells.

Applications Reported: This eBio4B10 (4B10) antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This eBio4B10 (4B10) antibody has been tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracellular (nuclear) proteins" located at www.thermofisher.com/flowprotocols . This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer ((Product # 00-8222-49)) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution ((Product # 00-5333-57)) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Target Information

T-bet, also known as TBX21, is a T-cell specific transcription factor containing a single T-box DNA-binding domain. It controls the expression of the TH1 cytokine, interferon-gamma. Both Th1 and Th17 cells are crucial in immune regulation and autoimmune disease development. T-bet initiates Th1 lineage development from naive Th precursor cells and directs T cell differentiation to Th1 versus Th17. T-bet is cooperating with Stat4 (signal tranducer and activator of transcription 4) in programming chromatin architecture for Th1 gene expression. Genetic variations in T-bet are associated with susceptibility to aspirin-induced asthma and nasal polyps.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Perinatal murine cytomegalovirus infection reshapes the transcriptional profile and functionality of NK cells.

Nature communications

Rožmanić C,Lisnić B,Pribanić Matešić M,Mihalić A,Hiršl L,Park E,Lesac Brizić A,Indenbirken D,Viduka I,Šantić M,Adler B,Yokoyama WM,Krmpotić A,Juranić Lisnić V,Jonjić S,Brizić I

Published figure using T-bet monoclonal antibody (Product # 15-5825-82) in Flow Cytometry

Thu Oct 12 00:00:00 EDT 2023

The Potential of Dendritic-Cell-Based Vaccines to Modulate Type 3 Innate Lymphoid Cell Populations.

International journal of molecular sciences

Chan L,Mehrani Y,Minott JA,Bridle BW,Karimi K

Published figure using T-bet monoclonal antibody (Product # 15-5825-82) in Flow Cytometry

Thu Jan 26 00:00:00 EST 2023

GM-CSF-activated human dendritic cells promote type 1 T follicular helper cell polarization in a CD40-dependent manner.

Journal of cell science

Korniotis S,Saichi M,Trichot C,Hoffmann C,Amblard E,Viguier A,Grondin S,Noel F,Mattoo H,Soumelis V

Published figure using T-bet monoclonal antibody (Product # 15-5825-82) in Flow Cytometry

Tue Nov 01 00:00:00 EDT 2022

Agr2-associated ER stress promotes adherent-invasive E. coli dysbiosis and triggers CD103⁺ dendritic cell IL-23-dependent ileocolitis.

Cell reports

Viladomiu M,Khounlotham M,Dogan B,Lima SF,Elsaadi A,Cardakli E,Castellanos JG,Ng C,Herzog J,Schoenborn AA,Ellermann M,Liu B,Zhang S,Gulati AS,Sartor RB,Simpson KW,Lipkin SM,Longman RS

Published figure using T-bet monoclonal antibody (Product # 15-5825-82) in Flow Cytometry

Tue Nov 15 00:00:00 EST 2022

Impact of secondary TCR engagement on the heterogeneity of pathogen-specific CD8+ T cell response during acute and chronic toxoplasmosis.

PLoS pathogens

Shallberg LA,Phan AT,Christian DA,Perry JA,Haskins BE,Beiting DP,Harris TH,Koshy AA,Hunter CA

15-5825-82 was used in flow cytometry to highlight that recent TCR activation contributes to the phenotypic heterogeneity of the CD8+ T cell response but suggest that this process has a limited impact on memory populations at acute and chronic stages of infection.

Wed Jun 01 00:00:00 EDT 2022

Engineered human cytokine/antibody fusion proteins expand regulatory T cells and confer autoimmune disease protection.

Cell reports

VanDyke D,Iglesias M,Tomala J,Young A,Smith J,Perry JA,Gebara E,Cross AR,Cheung LS,Dykema AG,Orcutt-Jahns BT,Henclová T,Golias J,Balolong J,Tomasovic LM,Funda D,Meyer AS,Pardoll DM,Hester J,Issa F,Hunter CA,Anderson MS,Bluestone JA,Raimondi G,Spangler JB

15-5825-82 was used in Flow cytometry/Cell sorting to provide a roadmap for IC design and establish a Treg-biased immunotherapy that could be clinically translated for autoimmune disease treatment.

Tue Oct 18 00:00:00 EDT 2022

ADAM12 is a costimulatory molecule that determines Th1 cell fate and mediates tissue inflammation.

Cellular & molecular immunology

Liu Y,Bockermann R,Hadi M,Safari I,Carrion B,Kveiborg M,Issazadeh-Navikas S

Published figure using T-bet monoclonal antibody (Product # 15-5825-82) in Flow Cytometry

Sun Aug 01 00:00:00 EDT 2021

Imbalance between T helper 1 and regulatory T cells plays a detrimental role in experimental Parkinson's disease in mice.

The Journal of international medical research

Li W,Luo Y,Xu H,Ma Q,Yao Q

Published figure using T-bet monoclonal antibody (Product # 15-5825-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Francisella tularensis induces Th1 like MAIT cells conferring protection against systemic and local infection.

Nature communications

Zhao Z,Wang H,Shi M,Zhu T,Pediongco T,Lim XY,Meehan BS,Nelson AG,Fairlie DP,Mak JYW,Eckle SBG,de Lima Moreira M,Tumpach C,Bramhall M,Williams CG,Lee HJ,Haque A,Evrard M,Rossjohn J,McCluskey J,Corbett AJ,Chen Z

Published figure using T-bet monoclonal antibody (Product # 15-5825-82) in Flow Cytometry

Fri Jul 16 00:00:00 EDT 2021

Heterologous arenavirus vector prime-boost overrules self-tolerance for efficient tumor-specific CD8 T cell attack.

Cell reports. Medicine

Bonilla WV,Kirchhammer N,Marx AF,Kallert SM,Krzyzaniak MA,Lu M,Darbre S,Schmidt S,Raguz J,Berka U,Vincenti I,Pauzuolis M,Kerber R,Hoepner S,Günther S,Magnus C,Merkler D,Orlinger KK,Zippelius A,Pinschewer DD

Published figure using T-bet monoclonal antibody (Product # 15-5825-82) in Flow Cytometry

Tue Mar 16 00:00:00 EDT 2021

Targeting Phosphatidylserine Enhances the Anti-tumor Response to Tumor-Directed Radiation Therapy in a Preclinical Model of Melanoma.

Cell reports

Budhu S,Giese R,Gupta A,Fitzgerald K,Zappasodi R,Schad S,Hirschhorn D,Campesato LF,De Henau O,Gigoux M,Liu C,Mazo G,Deng L,Barker CA,Wolchok JD,Merghoub T

Published figure using T-bet monoclonal antibody (Product # 15-5825-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Culture, expansion, and flow-cytometry-based functional analysis of pteropid bat MR1-restricted unconventional T cells.

STAR protocols

Sia WR,Hey YY,Foo R,Wang LF,Leeansyah E

Published figure using T-bet monoclonal antibody (Product # 15-5825-82) in Flow Cytometry

Fri Jun 18 00:00:00 EDT 2021

Graded RhoA GTPase Expression in Treg Cells Distinguishes Tumor Immunity From Autoimmunity.

Frontiers in immunology

Kalim KW,Yang JQ,Modur V,Nguyen P,Li Y,Zheng Y,Guo F

Published figure using T-bet monoclonal antibody (Product # 15-5825-82) in Flow Cytometry

Tue Jan 11 00:00:00 EST 2022

MiR-1165-3p Suppresses Th2 Differentiation via Targeting IL-13 and PPM1A in a Mouse Model of Allergic Airway Inflammation.

Allergy, asthma & immunology research

Wang Z,Ji N,Chen Z,Sun Z,Wu C,Yu W,Hu F,Huang M,Zhang M

Published figure using T-bet monoclonal antibody (Product # 15-5825-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2020

CD19-CAR-T Cells Bearing a KIR/PD-1-Based Inhibitory CAR Eradicate CD19⁺HLA-C1^- Malignant B Cells While Sparing CD19⁺HLA-C1⁺ Healthy B Cells.

Cancers

Tao L,Farooq MA,Gao Y,Zhang L,Niu C,Ajmal I,Zhou Y,He C,Zhao G,Yao J,Liu M,Jiang W

Published figure using T-bet monoclonal antibody (Product # 15-5825-82) in Flow Cytometry

Sun Sep 13 00:00:00 EDT 2020

Programming Multifaceted Pulmonary T Cell Immunity by Combination Adjuvants.

Cell reports. Medicine

Marinaik CB,Kingstad-Bakke B,Lee W,Hatta M,Sonsalla M,Larsen A,Neldner B,Gasper DJ,Kedl RM,Kawaoka Y,Suresh M

Published figure using T-bet monoclonal antibody (Product # 15-5825-82) in Flow Cytometry

Tue Sep 22 00:00:00 EDT 2020

Th2 Biased Immunity With Altered B Cell Profiles in Circulation of Patients With Sporotrichosis Caused by Sporothrix globosa.

Frontiers in immunology

Zu J,Yao L,Song Y,Cui Y,Guan M,Chen R,Zhen Y,Li S

Published figure using T-bet monoclonal antibody (Product # 15-5825-82) in Flow Cytometry

Mon May 31 00:00:00 EDT 2021

Adjuvant-free nanofiber vaccine induces in situ lung dendritic cell activation and T_H17 responses.

Science advances

Si Y,Tian Q,Zhao F,Kelly SH,Shores LS,Camacho DF,Sperling AI,Andrade MS,Collier JH,Chong AS

Published figure using T-bet monoclonal antibody (Product # 15-5825-82) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2020

FCRL5⁺ Memory B Cells Exhibit Robust Recall Responses.

Cell reports

Kim CC,Baccarella AM,Bayat A,Pepper M,Fontana MF

Published figure using T-bet monoclonal antibody (Product # 15-5825-82) in Flow Cytometry

Tue Apr 30 00:00:00 EDT 2019

Single-Cell Profiling Defines Transcriptomic Signatures Specific to Tumor-Reactive versus Virus-Responsive CD4⁺ T Cells.

Cell reports

Magen A,Nie J,Ciucci T,Tamoutounour S,Zhao Y,Mehta M,Tran B,McGavern DB,Hannenhalli S,Bosselut R

Published figure using T-bet monoclonal antibody (Product # 15-5825-82) in Flow Cytometry

Tue Dec 03 00:00:00 EST 2019

Bioinformatics

Protein Aliases: HGNC:11599; T-box 21; T-box expressed in T cells; T-box protein 21; T-box transcription factor TBX21; T-cell-specific T-box transcription factor T-bet; Transcription factor TBLYM

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Gene Aliases: T-bet; T-PET; TBET; TBLYM; TBT1; TBX21

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UniProt ID: (Human) Q9UL17, (Mouse) Q9JKD8

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Entrez Gene ID: (Rhesus monkey) 694044, (Human) 30009, (Mouse) 57765

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Function(s)

transcription factor activity, sequence-specific DNA binding transcription regulatory region DNA binding DNA binding protein binding Rel homology transcription factor

Process(es)

transcription, DNA-templated regulation of transcription, DNA-templated multicellular organism development response to virus T cell differentiation positive regulation of transcription, DNA-templated positive regulation of isotype switching to IgG isotypes cellular response to organic substance lymphocyte migration regulation of immune response

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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T-bet Monoclonal Antibody (eBio4B10 (4B10)), PE-Cyanine5, eBioscience™

T-bet Antibody (15-5825-82) in Flow

Product Details

15-5825-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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