Invitrogen

T-bet Monoclonal Antibody (eBio4B10 (4B10)), APC, eBioscience™

20 Published Figures

23 References

View all (31) T-bet antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (20)

FIGURE: 1 / 21

T-bet Antibody (17-5825-82) in Flow

Normal human peripheral blood cells were stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol, with CD8a Monoclonal Antibody, eFluor 450 (Product # 48-0087-42) and 0.125 µg of Mouse IgG1 kappa Isotype Control, APC (Product # 17-4714-82) (left) or 0.125 µg of T-bet Monoclonal Antibody, APC (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

17-5825-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 23 publications 23 publications

Product Specifications

Species Reactivity

Human, Mouse, Rhesus monkey

Published species

Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBio4B10 (4B10)

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2744712

Product Specific Information

Description: The eBio4B10 monoclonal antibody reacts with mouse and human T-bet. T-bet is a Th1-specific T-box transcription factor critical to the development of the Th1 CD4+ T cell lineage. This is known based on the observations that T-Bet deficient mice have impaired Th1 cell development, and that ectopic expression of T-Bet results in development skewed to the Th1 lineage. T-Bet expression is induced by the Th1 cytokine IFN gamma, and T-Bet also regulates the expression of IFN gamma, likely, at least in part, through the modification of DNA accessibility and histone remodeling. In addition to IFN gamma, T-Bet is also known to regulate the expression of IL-12R beta and IL-2. Moreover, T-Bet plays a role in class-switch recombination in B-cells.

Applications Reported: This eBio4B10 (4B10, 4-B10) antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This eBio4B10 (4B10, 4-B10) antibody has been tested by intracellular stainign and flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Buffer Set (Product # 00-5523-00) and protocol. Please see Best Protocol section; (refer to Protocol B: One step protocol for intracellular (nuclear) proteins). This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

T-bet, also known as TBX21, is a T-cell specific transcription factor containing a single T-box DNA-binding domain. It controls the expression of the TH1 cytokine, interferon-gamma. Both Th1 and Th17 cells are crucial in immune regulation and autoimmune disease development. T-bet initiates Th1 lineage development from naive Th precursor cells and directs T cell differentiation to Th1 versus Th17. T-bet is cooperating with Stat4 (signal tranducer and activator of transcription 4) in programming chromatin architecture for Th1 gene expression. Genetic variations in T-bet are associated with susceptibility to aspirin-induced asthma and nasal polyps.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Perinatal murine cytomegalovirus infection reshapes the transcriptional profile and functionality of NK cells.

Nature communications

Rožmanić C,Lisnić B,Pribanić Matešić M,Mihalić A,Hiršl L,Park E,Lesac Brizić A,Indenbirken D,Viduka I,Šantić M,Adler B,Yokoyama WM,Krmpotić A,Juranić Lisnić V,Jonjić S,Brizić I

Published figure using T-bet monoclonal antibody (Product # 17-5825-82) in Flow Cytometry

Thu Oct 12 00:00:00 EDT 2023

The Potential of Dendritic-Cell-Based Vaccines to Modulate Type 3 Innate Lymphoid Cell Populations.

International journal of molecular sciences

Chan L,Mehrani Y,Minott JA,Bridle BW,Karimi K

Published figure using T-bet monoclonal antibody (Product # 17-5825-82) in Flow Cytometry

Thu Jan 26 00:00:00 EST 2023

GM-CSF-activated human dendritic cells promote type 1 T follicular helper cell polarization in a CD40-dependent manner.

Journal of cell science

Korniotis S,Saichi M,Trichot C,Hoffmann C,Amblard E,Viguier A,Grondin S,Noel F,Mattoo H,Soumelis V

Published figure using T-bet monoclonal antibody (Product # 17-5825-82) in Flow Cytometry

Tue Nov 01 00:00:00 EDT 2022

Agr2-associated ER stress promotes adherent-invasive E. coli dysbiosis and triggers CD103⁺ dendritic cell IL-23-dependent ileocolitis.

Cell reports

Viladomiu M,Khounlotham M,Dogan B,Lima SF,Elsaadi A,Cardakli E,Castellanos JG,Ng C,Herzog J,Schoenborn AA,Ellermann M,Liu B,Zhang S,Gulati AS,Sartor RB,Simpson KW,Lipkin SM,Longman RS

Published figure using T-bet monoclonal antibody (Product # 17-5825-82) in Flow Cytometry

Tue Nov 15 00:00:00 EST 2022

MTHFD2 is a metabolic checkpoint controlling effector and regulatory T cell fate and function.

Immunity

Sugiura A,Andrejeva G,Voss K,Heintzman DR,Xu X,Madden MZ,Ye X,Beier KL,Chowdhury NU,Wolf MM,Young AC,Greenwood DL,Sewell AE,Shahi SK,Freedman SN,Cameron AM,Foerch P,Bourne T,Garcia-Canaveras JC,Karijolich J,Newcomb DC,Mangalam AK,Rabinowitz JD,Rathmell JC

17-5825-82 was used in Flow cytometry/Cell sorting to show that MTHFD2 is a metabolic checkpoint to integrate purine metabolism with pathogenic effector cell signaling and is a potential therapeutic target within 1C metabolism pathways.

Tue Jan 11 00:00:00 EST 2022

ADAM12 is a costimulatory molecule that determines Th1 cell fate and mediates tissue inflammation.

Cellular & molecular immunology

Liu Y,Bockermann R,Hadi M,Safari I,Carrion B,Kveiborg M,Issazadeh-Navikas S

Published figure using T-bet monoclonal antibody (Product # 17-5825-82) in Flow Cytometry

Sun Aug 01 00:00:00 EDT 2021

Imbalance between T helper 1 and regulatory T cells plays a detrimental role in experimental Parkinson's disease in mice.

The Journal of international medical research

Li W,Luo Y,Xu H,Ma Q,Yao Q

Published figure using T-bet monoclonal antibody (Product # 17-5825-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Francisella tularensis induces Th1 like MAIT cells conferring protection against systemic and local infection.

Nature communications

Zhao Z,Wang H,Shi M,Zhu T,Pediongco T,Lim XY,Meehan BS,Nelson AG,Fairlie DP,Mak JYW,Eckle SBG,de Lima Moreira M,Tumpach C,Bramhall M,Williams CG,Lee HJ,Haque A,Evrard M,Rossjohn J,McCluskey J,Corbett AJ,Chen Z

Published figure using T-bet monoclonal antibody (Product # 17-5825-82) in Flow Cytometry

Fri Jul 16 00:00:00 EDT 2021

Heterologous arenavirus vector prime-boost overrules self-tolerance for efficient tumor-specific CD8 T cell attack.

Cell reports. Medicine

Bonilla WV,Kirchhammer N,Marx AF,Kallert SM,Krzyzaniak MA,Lu M,Darbre S,Schmidt S,Raguz J,Berka U,Vincenti I,Pauzuolis M,Kerber R,Hoepner S,Günther S,Magnus C,Merkler D,Orlinger KK,Zippelius A,Pinschewer DD

Published figure using T-bet monoclonal antibody (Product # 17-5825-82) in Flow Cytometry

Tue Mar 16 00:00:00 EDT 2021

Targeting Phosphatidylserine Enhances the Anti-tumor Response to Tumor-Directed Radiation Therapy in a Preclinical Model of Melanoma.

Cell reports

Budhu S,Giese R,Gupta A,Fitzgerald K,Zappasodi R,Schad S,Hirschhorn D,Campesato LF,De Henau O,Gigoux M,Liu C,Mazo G,Deng L,Barker CA,Wolchok JD,Merghoub T

Published figure using T-bet monoclonal antibody (Product # 17-5825-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Culture, expansion, and flow-cytometry-based functional analysis of pteropid bat MR1-restricted unconventional T cells.

STAR protocols

Sia WR,Hey YY,Foo R,Wang LF,Leeansyah E

Published figure using T-bet monoclonal antibody (Product # 17-5825-82) in Flow Cytometry

Fri Jun 18 00:00:00 EDT 2021

Graded RhoA GTPase Expression in Treg Cells Distinguishes Tumor Immunity From Autoimmunity.

Frontiers in immunology

Kalim KW,Yang JQ,Modur V,Nguyen P,Li Y,Zheng Y,Guo F

Published figure using T-bet monoclonal antibody (Product # 17-5825-82) in Flow Cytometry

Tue Jan 11 00:00:00 EST 2022

Insulin-Like Growth Factors Are Key Regulators of T Helper 17 Regulatory T Cell Balance in Autoimmunity.

Immunity

DiToro D,Harbour SN,Bando JK,Benavides G,Witte S,Laufer VA,Moseley C,Singer JR,Frey B,Turner H,Bruning J,Darley-Usmar V,Gao M,Conover C,Hatton RD,Frank S,Colonna M,Weaver CT

17-5825 was used in Flow cytometry/Cell sorting to show that, among effector T cell subsets, Th17 and Treg cells selectively expressed multiple components of the IGF system.

Tue Apr 14 00:00:00 EDT 2020

Transcriptome dynamics of CD4⁺ T cells during malaria maps gradual transit from effector to memory.

Nature immunology

Soon MSF,Lee HJ,Engel JA,Straube J,Thomas BS,Pernold CPS,Clarke LS,Laohamonthonkul P,Haldar RN,Williams CG,Lansink LIM,Moreira ML,Bramhall M,Koufariotis LT,Wood S,Chen X,James KR,Lönnberg T,Lane SW,Belz GT,Engwerda CR,Khoury DS,Davenport MP,Svensson V,Teichmann SA,Haque A

17-5825 was used in Flow cytometry/Cell sorting to apply single-cell RNA sequencing and computational modelling to track memory development during Plasmodium infection and treatment.

Tue Dec 01 00:00:00 EST 2020

MiR-1165-3p Suppresses Th2 Differentiation via Targeting IL-13 and PPM1A in a Mouse Model of Allergic Airway Inflammation.

Allergy, asthma & immunology research

Wang Z,Ji N,Chen Z,Sun Z,Wu C,Yu W,Hu F,Huang M,Zhang M

Published figure using T-bet monoclonal antibody (Product # 17-5825-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2020

CD19-CAR-T Cells Bearing a KIR/PD-1-Based Inhibitory CAR Eradicate CD19⁺HLA-C1^- Malignant B Cells While Sparing CD19⁺HLA-C1⁺ Healthy B Cells.

Cancers

Tao L,Farooq MA,Gao Y,Zhang L,Niu C,Ajmal I,Zhou Y,He C,Zhao G,Yao J,Liu M,Jiang W

Published figure using T-bet monoclonal antibody (Product # 17-5825-82) in Flow Cytometry

Sun Sep 13 00:00:00 EDT 2020

Programming Multifaceted Pulmonary T Cell Immunity by Combination Adjuvants.

Cell reports. Medicine

Marinaik CB,Kingstad-Bakke B,Lee W,Hatta M,Sonsalla M,Larsen A,Neldner B,Gasper DJ,Kedl RM,Kawaoka Y,Suresh M

Published figure using T-bet monoclonal antibody (Product # 17-5825-82) in Flow Cytometry

Tue Sep 22 00:00:00 EDT 2020

Th2 Biased Immunity With Altered B Cell Profiles in Circulation of Patients With Sporotrichosis Caused by Sporothrix globosa.

Frontiers in immunology

Zu J,Yao L,Song Y,Cui Y,Guan M,Chen R,Zhen Y,Li S

Published figure using T-bet monoclonal antibody (Product # 17-5825-82) in Flow Cytometry

Mon May 31 00:00:00 EDT 2021

Adjuvant-free nanofiber vaccine induces in situ lung dendritic cell activation and T_H17 responses.

Science advances

Si Y,Tian Q,Zhao F,Kelly SH,Shores LS,Camacho DF,Sperling AI,Andrade MS,Collier JH,Chong AS

Published figure using T-bet monoclonal antibody (Product # 17-5825-82) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2020

FCRL5⁺ Memory B Cells Exhibit Robust Recall Responses.

Cell reports

Kim CC,Baccarella AM,Bayat A,Pepper M,Fontana MF

Published figure using T-bet monoclonal antibody (Product # 17-5825-82) in Flow Cytometry

Tue Apr 30 00:00:00 EDT 2019

Single-Cell Profiling Defines Transcriptomic Signatures Specific to Tumor-Reactive versus Virus-Responsive CD4⁺ T Cells.

Cell reports

Magen A,Nie J,Ciucci T,Tamoutounour S,Zhao Y,Mehta M,Tran B,McGavern DB,Hannenhalli S,Bosselut R

Published figure using T-bet monoclonal antibody (Product # 17-5825-82) in Flow Cytometry

Tue Dec 03 00:00:00 EST 2019

Neutrophil Cytosolic Factor 1 in Dendritic Cells Promotes Autoreactive CD8⁺ T Cell Activation via Cross-Presentation in Type 1 Diabetes.

Frontiers in immunology

Liu C,Whitener RL,Lin A,Xu Y,Chen J,Savinov A,Leiding JW,Wallet MA,Mathews CE

17-5825-82 was used in Flow Cytometry to demonstrate that Ncf1 (p47phox) is required for activation and effector function of CD8+ T cells by acting both intrinsically within the T cell as well as within professional antigen presenting cells.

Wed Sep 09 00:00:00 EDT 2020

Id2 regulates hyporesponsive invariant natural killer T cells.

Immunology and cell biology

Stradner MH,Cheung KP,Lasorella A,Goldrath AW,D'Cruz LM

17-5825 was used in Flow cytometry/Cell sorting to propose that downregulation of Id2 expression is essential to induction of the anti-inflammatory state in iNKT cells.

Mon Aug 01 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: HGNC:11599; T-box 21; T-box expressed in T cells; T-box protein 21; T-box transcription factor TBX21; T-cell-specific T-box transcription factor T-bet; Transcription factor TBLYM

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Gene Aliases: T-bet; T-PET; TBET; TBLYM; TBT1; TBX21

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UniProt ID: (Human) Q9UL17, (Mouse) Q9JKD8

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Entrez Gene ID: (Rhesus monkey) 694044, (Human) 30009, (Mouse) 57765

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Function(s)

transcription factor activity, sequence-specific DNA binding transcription regulatory region DNA binding DNA binding protein binding Rel homology transcription factor

Process(es)

transcription, DNA-templated regulation of transcription, DNA-templated multicellular organism development response to virus T cell differentiation positive regulation of transcription, DNA-templated positive regulation of isotype switching to IgG isotypes cellular response to organic substance lymphocyte migration regulation of immune response

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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T-bet Monoclonal Antibody (eBio4B10 (4B10)), APC, eBioscience™

T-bet Antibody (17-5825-82) in Flow

Product Details

17-5825-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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