Invitrogen

CD8b Monoclonal Antibody (eBioH35-17.2 (H35-17.2)), APC, eBioscience™

8 Published Figures

24 References

View all (76) CD8b antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (8)

FIGURE: 1 / 9

CD8b Antibody (17-0083-81) in Flow

Staining of C57Bl/6 splenocytes with Anti-Mouse CD8a FITC (Product # 11-0081-82) and 0.25 µg of Anti-Mouse CD8b APC. Total viable cells were used for analysis.

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Product Details

17-0083-81

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 24 publications 24 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioH35-17.2 (H35-17.2)

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_657760

Product Specific Information

Description: The eBioH35-17.2 monoclonal antibody reacts with the mouse CD8 beta molecule. The CD8 beta chain associates with the CD8 alpha chain to form the CD8 alpha/beta heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic alpha beta TCR T cells. CD8 binds to MHC class I and plays a role in T cell development and activation of mature T cells.

Applications Reported: This eBioH35-17.2 (H35-17.2) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioH35-17.2 (H35-17.2) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD8B antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

miR-4759 suppresses breast cancer through immune checkpoint blockade.

Computational and structural biotechnology journal

Lin YZ,Liu SH,Wu WR,Shen YC,Wang YL,Liao CC,Lin PL,Chang H,Liu LC,Cheng WC,Wang SC

17-0083-81 was used in Flow Cytometry to demonstrate miR-4759 as a novel non-coding RNA which promotes anti-tumor immunity of breast cancer.

Sat Jan 15 00:00:00 EST 2022

Protocol to isolate and enrich mouse splenic naive CD4⁺ T cells for in vitro CD4⁺CD8αα⁺ cell induction.

STAR protocols

Harada Y,Miyamoto K,Sujino T

17-0083-81 was used in Flow cytometry/Cell sorting to present an approach that can induce more CD4+CD8αα+ T cells by artificially creating a hypoxic environment in vitro.

Fri Dec 16 00:00:00 EST 2022

TCR-pMHC encounter differentially regulates transcriptomes of tissue-resident CD8 T cells.

European journal of immunology

Yoshizawa A,Bi K,Keskin DB,Zhang G,Reinhold B,Reinherz EL

17-0083-81 was used in Flow Cytometry to show TCR-pMHC interactions among TR and antigen presenting cells in a tissue milieu strongly impact CD8 T cell biology.

Mon Jan 01 00:00:00 EST 2018

Inhibiting Glycogen Synthase Kinase 3 Reverses Obesity-Induced White Adipose Tissue Inflammation by Regulating Apoptosis Inhibitor of Macrophage/CD5L-Mediated Macrophage Migration.

Arteriosclerosis, thrombosis, and vascular biology

Wang L,Wang Y,Zhang C,Li J,Meng Y,Dou M,Noguchi CT,Di L

17-0083-81 was used in Flow cytometry/Cell sorting to examine the potential mechanism by which GSK3 in modulating obesity-induced visceral adipose tissue (VAT) inflammation.

Sat Sep 01 00:00:00 EDT 2018

Peripheral administration of the soluble TNF inhibitor XPro1595 modifies brain immune cell profiles, decreases beta-amyloid plaque load, and rescues impaired long-term potentiation in 5xFAD mice.

Neurobiology of disease

MacPherson KP,Sompol P,Kannarkat GT,Chang J,Sniffen L,Wildner ME,Norris CM,Tansey MG

Published figure using CD8b monoclonal antibody (Product # 17-0083-81) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

Intratumoral modulation of the inducible co-stimulator ICOS by recombinant oncolytic virus promotes systemic anti-tumour immunity.

Nature communications

Zamarin D,Holmgaard RB,Ricca J,Plitt T,Palese P,Sharma P,Merghoub T,Wolchok JD,Allison JP

Published figure using CD8b monoclonal antibody (Product # 17-0083-81) in Flow Cytometry

Mon Feb 13 00:00:00 EST 2017

CD8⁺ T cells drive autoimmune hematopoietic stem cell dysfunction and bone marrow failure.

Journal of autoimmunity

Gravano DM,Al-Kuhlani M,Davini D,Sanders PD,Manilay JO,Hoyer KK

17-0083 was used in Flow cytometry/Cell sorting to investigate whether CD8+ T cells play a non-redundant role in driving bone marrow failure.

Thu Dec 01 00:00:00 EST 2016

Distinctive Expression of Bcl-2 Factors in Regulatory T Cells Determines a Pharmacological Target to Induce Immunological Tolerance.

Frontiers in immunology

Gabriel SS,Bon N,Chen J,Wekerle T,Bushell A,Fehr T,Cippà PE

17-0083 was used in Flow cytometry/Cell sorting to show that blocking the intrinsic apoptosis pathway with a Bcl-2/Bcl-xL inhibitor (ABT-737) caused a selective depletion of effector T cells and a relative enrichment of Tregs in vivo.

Mon Mar 14 00:00:00 EDT 2016

Growth and metastasis of lung adenocarcinoma is potentiated by BMP4-mediated immunosuppression.

Oncoimmunology

Chen L,Yi X,Goswami S,Ahn YH,Roybal JD,Yang Y,Diao L,Peng D,Peng D,Fradette JJ,Wang J,Byers LA,Kurie JM,Ullrich SE,Qin FX,Gibbons DL

17-0083 was used in Flow cytometry/Cell sorting to show that bone morphogenetic protein-4 causes immunosuppression by augmenting PD-L1 expression in the mesenchymal subset of lung cancer cells.

Tue Jan 12 00:00:00 EST 2021

Distinct Contributions of CD4+ and CD8+ T Cells to Pathogenesis of Trypanosoma brucei Infection in the Context of Gamma Interferon and Interleukin-10.

Infection and immunity

Liu G,Sun D,Wu H,Zhang M,Huan H,Xu J,Zhang X,Zhou H,Shi M

17-0083 was used in Flow cytometry/Cell sorting to study the distinct roles of CD4 and CD8 T cells in the context of IFN-γ and IL-10 during Trypanosoma brucei infections.

Wed Jul 01 00:00:00 EDT 2015

HGF Gene Modification in Mesenchymal Stem Cells Reduces Radiation-Induced Intestinal Injury by Modulating Immunity.

PloS one

Wang H,Sun RT,Li Y,Yang YF,Xiao FJ,Zhang YK,Wang SX,Sun HY,Zhang QW,Wu CT,Wang LS

17-0083 was used in Flow cytometry/Cell sorting to evaluate the therapeutic effect of hepatocyte growth factor-gene-modified MSCs on radiation-induced intestinal injury.

Tue Jan 26 00:00:00 EST 2016

Targeting the inhibitory receptor CTLA-4 on T cells increased abscopal effects in murine mesothelioma model.

Oncotarget

Wu L,Wu MO,De la Maza L,Yun Z,Yu J,Zhao Y,Cho J,de Perrot M

17-0083 was used in Flow cytometry/Cell sorting to investigate the local and abscopal effects of targeting the CTLA-4 T cell inhibitory receptor in a murine mesothelioma model.

Wed May 20 00:00:00 EDT 2015

The mammalian tRNA ligase complex mediates splicing of XBP1 mRNA and controls antibody secretion in plasma cells.

The EMBO journal

Jurkin J,Henkel T,Nielsen AF,Minnich M,Popow J,Kaufmann T,Heindl K,Hoffmann T,Busslinger M,Martinez J

17-0083 was used in Flow cytometry/Cell sorting to investigate the mammalian ligase required for cleavage of XBP1, showing that tRNA ligase complex mediates splicing of XBP1 mRNA.

Wed Dec 17 00:00:00 EST 2014

Metastasis is regulated via microRNA-200/ZEB1 axis control of tumour cell PD-L1 expression and intratumoral immunosuppression.

Nature communications

Chen L,Gibbons DL,Goswami S,Cortez MA,Ahn YH,Byers LA,Zhang X,Yi X,Dwyer D,Lin W,Diao L,Wang J,Roybal J,Patel M,Ungewiss C,Peng D,Antonia S,Mediavilla-Varela M,Robertson G,Suraokar M,Welsh JW,Erez B,Wistuba II,Chen L,Peng D,Wang S,Ullrich SE,Heymach JV,Kurie JM,Qin FX

Published figure using CD8b monoclonal antibody (Product # 17-0083-81) in Flow Cytometry

Tue Oct 28 00:00:00 EDT 2014

Inactivation of tumor-specific CD8⁺ CTLs by tumor-infiltrating tolerogenic dendritic cells.

Immunology and cell biology

Harimoto H,Shimizu M,Nakagawa Y,Nakatsuka K,Wakabayashi A,Sakamoto C,Takahashi H

17-0083 was used in Flow cytometry/Cell sorting to investigate cancer immunosurveillance failure caused by the inactivation of CD8+ cytotoxic T lymphocytes by tolerogenic dendritic cells.

Tue Oct 01 00:00:00 EDT 2013

Peptidoglycan recognition protein 1 enhances experimental asthma by promoting Th2 and Th17 and limiting regulatory T cell and plasmacytoid dendritic cell responses.

Journal of immunology (Baltimore, Md. : 1950)

Park SY,Jing X,Gupta D,Dziarski R

17-0083 was used in Flow cytometry/Cell sorting to demonstrate the proinflammatory effect of peptidoglycan recognition protein (Pglyrp)1 during the development of allergic asthma.

Mon Apr 01 00:00:00 EDT 2013

Deletion of Snai2 and Snai3 results in impaired physical development compounded by lymphocyte deficiency.

PloS one

Pioli PD,Dahlem TJ,Weis JJ,Weis JH

Published figure using CD8b monoclonal antibody (Product # 17-0083-81) in Flow Cytometry

Wed Feb 19 00:00:00 EST 2014

Defining the transcriptional and cellular landscape of type 1 diabetes in the NOD mouse.

PloS one

Carrero JA,Calderon B,Towfic F,Artyomov MN,Unanue ER

17-0083 was used in Flow cytometry/Cell sorting to examine autoimmune diabetes and its disease progression markers.

Mon Sep 30 00:00:00 EDT 2013

Up-regulation of a HOXA-PBX3 homeobox-gene signature following down-regulation of miR-181 is associated with adverse prognosis in patients with cytogenetically abnormal AML.

Blood

Li Z,Huang H,Li Y,Jiang X,Chen P,Arnovitz S,Radmacher MD,Maharry K,Elkahloun A,Yang X,He C,He M,Zhang Z,Dohner K,Neilly MB,Price C,Lussier YA,Zhang Y,Larson RA,Le Beau MM,Caligiuri MA,Bullinger L,Valk PJ,Delwel R,Lowenberg B,Liu PP,Marcucci G,Bloomfield CD,Rowley JD,Chen J

17-0083 was used in Flow cytometry/Cell sorting to study the prognostic impact of the four members of the miR-181 family in patients with CA-AML.

Thu Mar 08 00:00:00 EST 2012

The purinergic G protein-coupled receptor 6 inhibits effector T cell activation in allergic pulmonary inflammation.

Journal of immunology (Baltimore, Md. : 1950)

Giannattasio G,Ohta S,Boyce JR,Xing W,Balestrieri B,Boyce JA

17-0083 was used in Flow cytometry/Cell sorting to reveal the role of P2Y(6) receptors for protecting the lung against exuberant allergen-induced pulmonary inflammation.

Mon Aug 01 00:00:00 EDT 2011

TRAIL/DR5 plays a critical role in NK cell-mediated negative regulation of dendritic cell cross-priming of T cells.

Journal of immunology (Baltimore, Md. : 1950)

Iyori M,Zhang T,Pantel H,Gagne BA,Sentman CL

17-0083 was used in Flow cytometry/Cell sorting to indicate a novel alternative pathway for DC/NK cell interactions in antitumor immunity.

Thu Sep 15 00:00:00 EDT 2011

Immunization route dictates cross-priming efficiency and impacts the optimal timing of adjuvant delivery.

Frontiers in immunology

Bouvier I,Jusforgues-Saklani H,Lim A,Lemaître F,Lemercier B,Auriau C,Nicola MA,Leroy S,Law HK,Bandeira A,Moon JJ,Bousso P,Albert ML

17-0083 was used in Flow cytometry/Cell sorting to highlight the importance of considering the timing and persistence of antigen presentation for vaccination.

Thu Aug 23 00:00:00 EDT 2012

Replication allows inactivation of a knocked-in locus control region in inappropriate cell lineages.

Proceedings of the National Academy of Sciences of the United States of America

Menzel U,Ktistaki E,Tolaini M,Veiga-Fernandes H,Kioussis D

17-0083 was used in Flow cytometry/Cell sorting to explore the effect of a locus control region on a developmentally regulated locus in the mouse CD8 gene complex.

Tue Sep 28 00:00:00 EDT 2010

Expression of the chemokine binding protein M3 promotes marked changes in the accumulation of specific leukocytes subsets within the intestine.

Gastroenterology

Shang L,Thirunarayanan N,Viejo-Borbolla A,Martin AP,Bogunovic M,Marchesi F,Unkeless JC,Ho Y,Furtado GC,Alcami A,Merad M,Mayer L,Lira SA

Published figure using CD8b monoclonal antibody (Product # 17-0083-81) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: CD8; CD8alpha; CD8b; CD8beta; Leu-2; Lymphocyte antigen 3; MAL; T-cell membrane glycoprotein Ly-3; T-cell surface glycoprotein CD8 beta chain; T-cell surface glycoprotein Lyt-3

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Gene Aliases: Cd8b; Cd8b1; Ly-3; Ly-C; Lyt-3; Lyt3

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UniProt ID: (Mouse) P10300

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Entrez Gene ID: (Mouse) 12526

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Function(s)

protein binding defense/immunity protein immunoglobulin receptor superfamily

Process(es)

immune system process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD8b Monoclonal Antibody (eBioH35-17.2 (H35-17.2)), APC, eBioscience™

CD8b Antibody (17-0083-81) in Flow

Product Details

17-0083-81

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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