Invitrogen

CD8b Monoclonal Antibody (eBioH35-17.2 (H35-17.2)), PE, eBioscience™

8 Published Figures

14 References

View all (76) CD8b antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (8)

FIGURE: 1 / 9

CD8b Antibody (12-0083-82) in Flow

Staining of C57Bl/6 splenocytes with Anti-Mouse CD8a APC (Product # 17-0081-82) and 0.06 µg of Rat IgG2b K Isotype Control PE (Product # 12-4031-82) (left) or 0.06 µg of Anti-Mouse CD8b PE (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

12-0083-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 13 publications 13 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioH35-17.2 (H35-17.2)

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_657767

Product Specific Information

Description: The eBioH35-17.2 monoclonal antibody reacts with the mouse CD8 beta molecule. The CD8 beta chain associates with the CD8 alpha chain to form the CD8 alpha/beta heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic alpha beta TCR T cells. CD8 binds to MHC class I and plays a role in T cell development and activation of mature T cells.

Applications Reported: This eBioH35-17.2 (H35-17.2) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioH35-17.2 (H35-17.2) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD8B antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Heterogenous Populations of Tissue-Resident CD8⁺ T Cells Are Generated in Response to Infection and Malignancy.

Immunity

Milner JJ,Toma C,He Z,Kurd NS,Nguyen QP,McDonald B,Quezada L,Widjaja CE,Witherden DA,Crowl JT,Shaw LA,Yeo GW,Chang JT,Omilusik KD,Goldrath AW

12-0083 was used in Flow cytometry/Cell sorting to identify discrete lineages of intestinal antigen-specific CD8+ T cells, including a Blimp1hiId3lo tissue-resident effector cell population most prominent in the early phase of acute viral and bacterial infections and a molecularly distinct Blimp1loId3hi tissue-resident memory population that subsequently accumulated at later infection time points.

Tue May 19 00:00:00 EDT 2020

The Bone Marrow Protects and Optimizes Immunological Memory during Dietary Restriction.

Cell

Collins N,Han SJ,Enamorado M,Link VM,Huang B,Moseman EA,Kishton RJ,Shannon JP,Dixit D,Schwab SR,Hickman HD,Restifo NP,McGavern DB,Schwartzberg PL,Belkaid Y

12-0083-82 was used in Flow Cytometry to show that memory T cells collapsed in secondary lymphoid organs in the context of dietary restriction (DR) but dramatically accumulated within the bone marrow (BM), where they adopted a state associated with energy conservation.

Thu Aug 22 00:00:00 EDT 2019

Aryl hydrocarbon receptor activation modulates γδ intestinal intraepithelial lymphocytes and protects against ischemia/reperfusion injury in the murine small intestine.

Molecular medicine reports

Zhang Z,Pu A,Yu M,Xiao W,Sun L,Cai Y,Yang H

12-0083-82 was used in Flow cytometry/Cell sorting to suggest that the AhR plays an important role in protecting the small intestine from I/R and increasing the γδIEL population by decreasing apoptosis of γδIELs.

Fri Mar 01 00:00:00 EST 2019

Peripheral administration of the soluble TNF inhibitor XPro1595 modifies brain immune cell profiles, decreases beta-amyloid plaque load, and rescues impaired long-term potentiation in 5xFAD mice.

Neurobiology of disease

MacPherson KP,Sompol P,Kannarkat GT,Chang J,Sniffen L,Wildner ME,Norris CM,Tansey MG

Published figure using CD8b monoclonal antibody (Product # 12-0083-82) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

Intratumoral modulation of the inducible co-stimulator ICOS by recombinant oncolytic virus promotes systemic anti-tumour immunity.

Nature communications

Zamarin D,Holmgaard RB,Ricca J,Plitt T,Palese P,Sharma P,Merghoub T,Wolchok JD,Allison JP

Published figure using CD8b monoclonal antibody (Product # 12-0083-82) in Flow Cytometry

Mon Feb 13 00:00:00 EST 2017

Growth and metastasis of lung adenocarcinoma is potentiated by BMP4-mediated immunosuppression.

Oncoimmunology

Chen L,Yi X,Goswami S,Ahn YH,Roybal JD,Yang Y,Diao L,Peng D,Peng D,Fradette JJ,Wang J,Byers LA,Kurie JM,Ullrich SE,Qin FX,Gibbons DL

12-0083 was used in Flow cytometry/Cell sorting to show that bone morphogenetic protein-4 causes immunosuppression by augmenting PD-L1 expression in the mesenchymal subset of lung cancer cells.

Tue Jan 12 00:00:00 EST 2021

Tumor sialylation impedes T cell mediated anti-tumor responses while promoting tumor associated-regulatory T cells.

Oncotarget

Perdicchio M,Cornelissen LA,Streng-Ouwehand I,Engels S,Verstege MI,Boon L,Geerts D,van Kooyk Y,Unger WW

12-0083 was used in Flow cytometry/Cell sorting to study the influence of B16 melanoma hypersialylation on tumour growth.

Tue Feb 23 00:00:00 EST 2016

Myeloid Cell Arg1 Inhibits Control of Arthritogenic Alphavirus Infection by Suppressing Antiviral T Cells.

PLoS pathogens

Burrack KS,Tan JJ,McCarthy MK,Her Z,Berger JN,Ng LF,Morrison TE

12-0083 was used in Flow cytometry/Cell sorting to explore the relationship between viral load in CHIKV-infected patients and expression of Arg1.

Thu Oct 01 00:00:00 EDT 2015

CD8+ T cells control Ross River virus infection in musculoskeletal tissues of infected mice.

Journal of immunology (Baltimore, Md. : 1950)

Burrack KS,Montgomery SA,Homann D,Morrison TE

12-0083 was used in Flow cytometry/Cell sorting to demonstrate an important but limited and tissue-specific role for CD8+ T lymphocytes in the control of pathogenic RRV infection.

Thu Jan 15 00:00:00 EST 2015

The In Vivo Granulopoietic Response to Dexamethasone Injection Is Abolished in Perforin-Deficient Mutant Mice and Corrected by Lymphocyte Transfer from Nonsensitized Wild-Type Donors.

Mediators of inflammation

Xavier-Elsas P,da Silva CL,Vieira BM,Masid-de-Brito D,Queto T,de Luca B,Vieira TS,Gaspar-Elsas MI

12-0083-82 was used in Immunodepletion to demonstrate that the perforin-dependent, granulopoietic response to dexamethasone can be restored by transfer of innate lymphocyte subpopulations.

Fri Feb 12 00:00:00 EST 2016

Metastasis is regulated via microRNA-200/ZEB1 axis control of tumour cell PD-L1 expression and intratumoral immunosuppression.

Nature communications

Chen L,Gibbons DL,Goswami S,Cortez MA,Ahn YH,Byers LA,Zhang X,Yi X,Dwyer D,Lin W,Diao L,Wang J,Roybal J,Patel M,Ungewiss C,Peng D,Antonia S,Mediavilla-Varela M,Robertson G,Suraokar M,Welsh JW,Erez B,Wistuba II,Chen L,Peng D,Wang S,Ullrich SE,Heymach JV,Kurie JM,Qin FX

12-0083 was used in Flow cytometry/Cell sorting to reveal a link between EMT and T-cell dysfunction and show that ZEB1 promotes metastasis through a cell non-autonomous mechanism.

Tue Oct 28 00:00:00 EDT 2014

Human IL-2 mutein with higher antitumor efficacy than wild type IL-2.

Journal of immunology (Baltimore, Md. : 1950)

Carmenate T,Pacios A,Enamorado M,Moreno E,Garcia-Martínez K,Fuente D,León K

12-0083 was used in Flow cytometry/Cell sorting to characterise a human IL-2 mutant for used intreatment of melanoma and renal cell carcinoma, showing that mutant IL-2 has a higher antitumor efficacy and lower toxicity than wild type IL-2.

Sat Jun 15 00:00:00 EDT 2013

Deletion of Snai2 and Snai3 results in impaired physical development compounded by lymphocyte deficiency.

PloS one

Pioli PD,Dahlem TJ,Weis JJ,Weis JH

12-0083 was used in Flow cytometry/Cell sorting to investigate the function of Snai3, demonstrating its partial functional redundancy.

Wed Feb 19 00:00:00 EST 2014

Expression of the chemokine binding protein M3 promotes marked changes in the accumulation of specific leukocytes subsets within the intestine.

Gastroenterology

Shang L,Thirunarayanan N,Viejo-Borbolla A,Martin AP,Bogunovic M,Marchesi F,Unkeless JC,Ho Y,Furtado GC,Alcami A,Merad M,Mayer L,Lira SA

Published figure using CD8b monoclonal antibody (Product # 12-0083-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: CD8; CD8alpha; CD8b; CD8beta; Leu-2; Lymphocyte antigen 3; MAL; T-cell membrane glycoprotein Ly-3; T-cell surface glycoprotein CD8 beta chain; T-cell surface glycoprotein Lyt-3

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Gene Aliases: Cd8b; Cd8b1; Ly-3; Ly-C; Lyt-3; Lyt3

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UniProt ID: (Mouse) P10300

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Entrez Gene ID: (Mouse) 12526

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Function(s)

protein binding defense/immunity protein immunoglobulin receptor superfamily

Process(es)

immune system process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD8b Monoclonal Antibody (eBioH35-17.2 (H35-17.2)), PE, eBioscience™

CD8b Antibody (12-0083-82) in Flow

Product Details

12-0083-82

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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