Invitrogen

CD69 Monoclonal Antibody (H1.2F3), APC, eBioscience™

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Antibody Testing Data (1)
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CD69 Antibody (17-0691-82) in Flow

Staining of 1-day ConA-stimulated BALB/c splenocytes with 0.125 µg of Armenian Hamster IgG Isotype Control APC (Product # 17-4888-82) (blue histogram) or 0.125 µg of Anti-Mouse CD69 APC (purple histogram). Total viable cells were used for analysis.

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Product Details

17-0691-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 83 publications 83 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

H1.2F3

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1210795

Product Specific Information

Description: The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen (VEA). CD69 is approximately 35 kDa and is expressed on the surface as a disulfide-linked dimer. While a small subset of lymphocytes in the thymus, spleen and lymph nodes express this antigen, activation of both T and B cells rapidly upregulates the surface expression of CD69, suggesting a role for CD69 in lymphocyte development and activation.

Applications Reported: This H1.2F3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This H1.2F3 antibody has been tested by flow cytometric analysis of stimulated mouse splenic cell cultures. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide bonded homodimer with a molecular weight of 60 kDa under non-reducing conditions. CD69 contains one or two N linked oligosacaride and the molecule is present on activated platelets. In normal peripheral blood a variable percentage of cells express the CD69 antigen, and it is involved in lymphocyte signal transduction. Expression CD69 is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. Alternative splicing results in multiple transcript variants of CD69. Diseases associated with CD69 dysfunction include coccidiodomycosis and asthma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

HSF1 promotes CD69⁺ Treg differentiation to inhibit colitis progression.

Theranostics

Yu L,Zhou B,Zhu Y,Li L,Zhong Y,Zhu L,Wang H,Chen H,Xu J,Guo T,Feng L,Wang X,Cai Z,Wang J,Jin H

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Tue Apr 18 00:00:00 EDT 2023

Modulating CD40 and integrin signaling in the proinflammatory nexus using a 15-amino-acid peptide, KGYY₁₅.

The Journal of biological chemistry

Vaitaitis GM,Wagner DH

17-0691-82 was used in Flow cytometry/Cell sorting to demonstrate interactions between CD40 and integrin CD11a/CD18, which complicates the understanding of the inflammatory nexus and how to prevent autoinflammation.

Mon May 01 00:00:00 EDT 2023

Novel Bruton's tyrosine kinase inhibitor TAS5315 suppresses the progression of inflammation and joint destruction in rodent collagen-induced arthritis.

PloS one

Akasaka D,Iguchi S,Kaneko R,Yoshiga Y,Kajiwara D,Nakachi Y,Noma N,Tanaka K,Shimizu A,Hosoi F

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Mon Feb 27 00:00:00 EST 2023

TCR repertoire landscape reveals macrophage-mediated clone deletion in endotoxin tolerance.

Inflammation research : official journal of the European Histamine Research Society ... [et al.]

Zhao J,Jia L,Tao Y,Zhao X,Yang J,Lu Y,Yan Y,Mao L,Hu L,Lu J,Guo M,Chen C,Zhou Y,Wen Z,He Z,Xu L

17-0691-82 was used in Flow cytometry/Cell sorting to suggest that the clone selection in thymus in Endotoxin tolerance may confer protection against microbial sepsis.

Wed Mar 01 00:00:00 EST 2023

Mesenchymal stromal cells equipped by IFNα empower T cells with potent anti-tumor immunity.

Oncogene

Zhang T,Wang Y,Li Q,Lin L,Xu C,Xue Y,Hu M,Shi Y,Wang Y

17-0691-82 was used in Flow Cytometry to conclude that IFNα-MSCs represent a novel tumor immunotherapy strategy, especially when combined with PD-L1 blockade.

Tue Mar 01 00:00:00 EST 2022

Intranasal Immunization With a c-di-GMP-Adjuvanted Acellular Pertussis Vaccine Provides Superior Immunity Against Bordetella pertussis in a Mouse Model.

Frontiers in immunology

Jiang W,Wang X,Su Y,Cai L,Li J,Liang J,Gu Q,Sun M,Shi L

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Tue May 03 00:00:00 EDT 2022

Meningeal lymphatic drainage promotes T cell responses against Toxoplasma gondii but is dispensable for parasite control in the brain.

eLife

Kovacs MA,Cowan MN,Babcock IW,Sibley LA,Still K,Batista SJ,Labuzan SA,Sethi I,Harris TH

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Wed Dec 21 00:00:00 EST 2022

An autonomous TCR signal-sensing switch influences CD4/CD8 lineage choice in mice.

Communications biology

Basu J,Zha J,Nicolas E,Coulton M,Czyzewicz P,Hua X,Ge L,Kappes DJ

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Fri Jan 21 00:00:00 EST 2022

CP-25 exerts a protective effect against ConA-induced hepatitis via regulating inflammation and immune response.

Frontiers in pharmacology

Li N,Wu JJ,Qi M,Wang ZY,Zhang SN,Li XQ,Chen TT,Wang MF,Zhang LL,Wei W,Sun WY

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Sat Dec 03 00:00:00 EST 2022

A novel costimulatory molecule gene-modified leukemia cell-derived exosome-targeted CD4⁺ T cell vaccine efficiently enhances anti-leukemia immunity.

Frontiers in immunology

Li J,Huang F,Jiang Y,Zhao J,Wan J,Hao S

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Tue Dec 06 00:00:00 EST 2022

ADAM12 is a costimulatory molecule that determines Th1 cell fate and mediates tissue inflammation.

Cellular & molecular immunology

Liu Y,Bockermann R,Hadi M,Safari I,Carrion B,Kveiborg M,Issazadeh-Navikas S

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Sun Aug 01 00:00:00 EDT 2021

Uncovering a novel role of PLCβ4 in selectively mediating TCR signaling in CD8+ but not CD4+ T cells.

The Journal of experimental medicine

Sasai M,Ma JS,Okamoto M,Nishino K,Nagaoka H,Takashima E,Pradipta A,Lee Y,Kosako H,Suh PG,Yamamoto M

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Mon Jul 05 00:00:00 EDT 2021

Fasudil prevents liver fibrosis via activating natural killer cells and suppressing hepatic stellate cells.

World journal of gastroenterology

Han QJ,Mu YL,Zhao HJ,Zhao RR,Guo QJ,Su YH,Zhang J

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Mon Jun 28 00:00:00 EDT 2021

TRPM8 channel augments T-cell activation and proliferation.

Cell biology international

Acharya TK,Tiwari A,Majhi RK,Goswami C

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Cell-permeable transgelin-2 as a potent therapeutic for dendritic cell-based cancer immunotherapy.

Journal of hematology & oncology

Kim HR,Park JS,Park JH,Yasmin F,Kim CH,Oh SK,Chung IJ,Jun CD

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Wed Mar 17 00:00:00 EDT 2021

Suppression of mucosal Th17 memory responses by acellular pertussis vaccines enhances nasal Bordetella pertussis carriage.

NPJ vaccines

Dubois V,Chatagnon J,Thiriard A,Bauderlique-Le Roy H,Debrie AS,Coutte L,Locht C

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Fri Jan 08 00:00:00 EST 2021

STING Activated Tumor-Intrinsic Type I Interferon Signaling Promotes CXCR3 Dependent Antitumor Immunity in Pancreatic Cancer.

Cellular and molecular gastroenterology and hepatology

Vonderhaar EP,Barnekow NS,McAllister D,McOlash L,Eid MA,Riese MJ,Tarakanova VL,Johnson BD,Dwinell MB

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Mon Jan 24 00:00:00 EST 2022

GPR120 induces regulatory dendritic cells by inhibiting HK2-dependent glycolysis to alleviate fulminant hepatic failure.

Cell death & disease

Yu H,Yang W,Huang J,Miao X,Wang B,Ren X,Gu Y,Wang Q,Ding X,Guo X,Qian F,Zhang Y,Xu H,Zheng L,Jin M

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Thu Dec 16 00:00:00 EST 2021

Robust induction of T_RMs by combinatorial nanoshells confers cross-strain sterilizing immunity against lethal influenza viruses.

Molecular therapy. Methods & clinical development

Lin PH,Liang CY,Yao BY,Chen HW,Pan CF,Wu LL,Lin YH,Hsu YS,Liu YH,Chen PJ,Hu CJ,Yang HC

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Fri Jun 11 00:00:00 EDT 2021

SPARC regulation of PMN clearance protects from pristane-induced lupus and rheumatoid arthritis.

iScience

Sangaletti S,Botti L,Gulino A,Lecis D,Bassani B,Portararo P,Milani M,Cancila V,De Cecco L,Dugo M,Tripodo C,Colombo MP

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Fri Jun 25 00:00:00 EDT 2021

Hybrid Gene Origination Creates Human-Virus Chimeric Proteins during Infection.

Cell

Ho JSY,Angel M,Ma Y,Sloan E,Wang G,Martinez-Romero C,Alenquer M,Roudko V,Chung L,Zheng S,Chang M,Fstkchyan Y,Clohisey S,Dinan AM,Gibbs J,Gifford R,Shen R,Gu Q,Irigoyen N,Campisi L,Huang C,Zhao N,Jones JD,van Knippenberg I,Zhu Z,Moshkina N,Meyer L,Noel J,Peralta Z,Rezelj V,Kaake R,Rosenberg B,Wang B,Wei J,Paessler S,Wise HM,Johnson J,Vannini A,Amorim MJ,Baillie JK,Miraldi ER,Benner C,Brierley I,Digard P,Łuksza M,Firth AE,Krogan N,Greenbaum BD,MacLeod MK,van Bakel H,Garcìa-Sastre A,Yewdell JW,Hutchinson E,Marazzi I

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Thu Jun 25 00:00:00 EDT 2020

Microglia Require CD4 T Cells to Complete the Fetal-to-Adult Transition.

Cell

Pasciuto E,Burton OT,Roca CP,Lagou V,Rajan WD,Theys T,Mancuso R,Tito RY,Kouser L,Callaerts-Vegh Z,de la Fuente AG,Prezzemolo T,Mascali LG,Brajic A,Whyte CE,Yshii L,Martinez-Muriana A,Naughton M,Young A,Moudra A,Lemaitre P,Poovathingal S,Raes J,De Strooper B,Fitzgerald DC,Dooley J,Liston A

17-0691 was used in Flow cytometry/Cell sorting to investigate the function of brain-resident CD4 T cells.

Thu Aug 06 00:00:00 EDT 2020

A Hyper-IgM Syndrome Mutation in Activation-Induced Cytidine Deaminase Disrupts G-Quadruplex Binding and Genome-wide Chromatin Localization.

Immunity

Yewdell WT,Kim Y,Chowdhury P,Lau CM,Smolkin RM,Belcheva KT,Fernandez KC,Cols M,Yen WF,Vaidyanathan B,Angeletti D,McDermott AB,Yewdell JW,Sun JC,Chaudhuri J

17-0691-82 was used in Flow cytometry/Cell sorting to indicate a crucial role for G4 binding in AID targeting and suggest that AID activity may extend beyond Ig loci to regulate the expression of genes relevant to the physiology and pathology of activated B cells.

Tue Nov 17 00:00:00 EST 2020

Contact-dependent delivery of IL-2 by dendritic cells to CD4 T cells in the contraction phase promotes their long-term survival.

Protein & cell

Tong D,Zhang L,Ning F,Xu Y,Hu X,Shi Y

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Sat Feb 01 00:00:00 EST 2020

Podocytes present antigen to activate specific T cell immune responses in inflammatory renal disease.

The Journal of pathology

Li S,Liu Y,He Y,Rong W,Zhang M,Li L,Liu Z,Zen K

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

Increase in liver γδ T cells with concurrent augmentation of IFN-β production during the early stages of a mouse model of acute experimental hepatitis B virus infection.

Experimental and therapeutic medicine

Chang L,Wang L,Ling N,Peng H,Chen M

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2020

Effects of CD4⁺ T lymphocytes from ovariectomized mice on bone marrow mesenchymal stem cell proliferation and osteogenic differentiation.

Experimental and therapeutic medicine

Shao BY,Wang L,Yu Y,Chen L,Gan N,Huang WM

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2020

Potentiating the Antitumor Activity of Cytotoxic T Cells via the Transmembrane Domain of IGSF4 That Increases TCR Avidity.

Frontiers in immunology

Kim HR,Park JS,Fatima Y,Kausar M,Park JH,Jun CD

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Tue Jul 27 00:00:00 EDT 2021

Targeting the orphan nuclear receptor NR2F6 in T cells primes tumors for immune checkpoint therapy.

Cell communication and signaling : CCS

Klepsch V,Pommermayr M,Humer D,Brigo N,Hermann-Kleiter N,Baier G

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Tue Jan 14 00:00:00 EST 2020

SLAMF6 deficiency augments tumor killing and skews toward an effector phenotype revealing it as a novel T cell checkpoint.

eLife

Hajaj E,Eisenberg G,Klein S,Frankenburg S,Merims S,Ben David I,Eisenhaure T,Henrickson SE,Villani AC,Hacohen N,Abudi N,Abramovich R,Cohen JE,Peretz T,Veillette A,Lotem M

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Tue Mar 03 00:00:00 EST 2020

Loss of testosterone impairs anti-tumor neutrophil function.

Nature communications

Markman JL,Porritt RA,Wakita D,Lane ME,Martinon D,Noval Rivas M,Luu M,Posadas EM,Crother TR,Arditi M

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Tue Mar 31 00:00:00 EDT 2020

Identification of atypical mitogen-activated protein kinase MAPK4 as a novel regulator in acute lung injury.

Cell & bioscience

Mao L,Zhou Y,Chen L,Hu L,Liu S,Zheng W,Zhao J,Guo M,Chen C,He Z,Xu L

17-0691-82 was used in Flow Cytometry to reveal an unknown role of atypical MAPKs member MAPK4 in the pathology of acute lung injury.

Sun Apr 17 00:00:00 EDT 2022

MIR205HG Is a Long Noncoding RNA that Regulates Growth Hormone and Prolactin Production in the Anterior Pituitary.

Developmental cell

Du Q,Hoover AR,Dozmorov I,Raj P,Khan S,Molina E,Chang TC,de la Morena MT,Cleaver OB,Mendell JT,van Oers NSC

17-0691-80 was used in Flow Cytometry to show growth hormone and prolactin production in the anterior pituitary is regulated by the long non-coding RNA MIR205HG.

Mon May 20 00:00:00 EDT 2019

CSF1R antagonism limits local restimulation of antiviral CD8⁺ T cells during viral encephalitis.

Journal of neuroinflammation

Funk KE,Klein RS

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Thu Jan 31 00:00:00 EST 2019

TCR and Inflammatory Signals Tune Human MAIT Cells to Exert Specific Tissue Repair and Effector Functions.

Cell reports

Leng T,Akther HD,Hackstein CP,Powell K,King T,Friedrich M,Christoforidou Z,McCuaig S,Neyazi M,Arancibia-Cárcamo CV,Hagel J,Powrie F,Peres RS,Millar V,Ebner D,Lamichhane R,Ussher J,Hinks TSC,Marchi E,Willberg C,Klenerman P

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Tue Sep 17 00:00:00 EDT 2019

Enhanced germinal center reaction by targeting vaccine antigen to major histocompatibility complex class II molecules.

NPJ vaccines

Andersen TK,Huszthy PC,Gopalakrishnan RP,Jacobsen JT,Fauskanger M,Tveita AA,Grødeland G,Bogen B

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Thu Oct 05 00:00:00 EDT 2023

Noc4L-Mediated Ribosome Biogenesis Controls Activation of Regulatory and Conventional T Cells.

Cell reports

Zhu X,Zhang W,Guo J,Zhang X,Li L,Wang T,Yan J,Zhang F,Hou B,Gao N,Gao GF,Zhou X

17-0691 was used in Flow cytometry/Cell sorting to identify the control of Treg activation and immune tolerance maintenance is due to Noc4L mediated ribosome biogenesis.

Tue Apr 23 00:00:00 EDT 2019

Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells.

Cell

Gaya M,Barral P,Burbage M,Aggarwal S,Montaner B,Warren Navia A,Aid M,Tsui C,Maldonado P,Nair U,Ghneim K,Fallon PG,Sekaly RP,Barouch DH,Shalek AK,Bruckbauer A,Strid J,Batista FD

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Thu Jan 25 00:00:00 EST 2018

A Pck1-directed glycogen metabolic program regulates formation and maintenance of memory CD8⁺ T cells.

Nature cell biology

Ma R,Ji T,Zhang H,Dong W,Chen X,Xu P,Chen D,Liang X,Yin X,Liu Y,Ma J,Tang K,Zhang Y,Peng Y,Lu J,Zhang Y,Qin X,Cao X,Wan Y,Huang B

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Mon Jan 01 00:00:00 EST 2018

Intranasal delivery of adjuvant-free peptide nanofibers elicits resident CD8⁺ T cell responses.

Journal of controlled release : official journal of the Controlled Release Society

Si Y,Wen Y,Kelly SH,Chong AS,Collier JH

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Sat Jul 28 00:00:00 EDT 2018

MicroRNA-126 deficiency enhanced the activation and function of CD4⁺ T cells by elevating IRS-1 pathway.

Clinical and experimental immunology

Chu F,Hu Y,Zhou Y,Guo M,Lu J,Zheng W,Xu H,Zhao J,Xu L

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Thu Feb 01 00:00:00 EST 2018

Oncofetal gene SALL4 reactivation by hepatitis B virus counteracts miR-200c in PD-L1-induced T cell exhaustion.

Nature communications

Sun C,Lan P,Han Q,Huang M,Zhang Z,Xu G,Song J,Wang J,Wei H,Zhang J,Sun R,Zhang C,Tian Z

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Wed Mar 28 00:00:00 EDT 2018

MAIT cells contribute to protection against lethal influenza infection in vivo.

Nature communications

van Wilgenburg B,Loh L,Chen Z,Pediongco TJ,Wang H,Shi M,Zhao Z,Koutsakos M,Nüssing S,Sant S,Wang Z,D'Souza C,Jia X,Almeida CF,Kostenko L,Eckle SBG,Meehan BS,Kallies A,Godfrey DI,Reading PC,Corbett AJ,McCluskey J,Klenerman P,Kedzierska K,Hinks TSC

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Fri Nov 09 00:00:00 EST 2018

Homoharringtonine induced immune alteration for an Efficient Anti-tumor Response in Mouse Models of Non-small Cell Lung Adenocarcinoma Expressing Kras Mutation.

Scientific reports

Weng TY,Wu HF,Li CY,Hung YH,Chang YW,Chen YL,Hsu HP,Chen YH,Wang CY,Chang JY,Lai MD

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Tue May 29 00:00:00 EDT 2018

Development of a screening strategy for new modulators of T cell receptor signaling and T cell activation.

Scientific reports

Chen EW,Brzostek J,Gascoigne NRJ,Rybakin V

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Tue Jul 03 00:00:00 EDT 2018

A method for manual and automated multiplex RNAscope in situ hybridization and immunocytochemistry on cytospin samples.

PloS one

Chan S,Filézac de L'Etang A,Rangell L,Caplazi P,Lowe JB,Romeo V

17-0691 was used in Flow cytometry/Cell sorting to develop a method for manual and automated multiplex RNAscope in situ hybridisation and immunocytochemistry on cytospin samples.

Mon Apr 22 00:00:00 EDT 2019

The Cyclopeptide Astin C Specifically Inhibits the Innate Immune CDN Sensor STING.

Cell reports

Li S,Hong Z,Wang Z,Li F,Mei J,Huang L,Lou X,Zhao S,Song L,Chen W,Wang Q,Liu H,Cai Y,Yu H,Xu H,Zeng G,Wang Q,Zhu J,Liu X,Tan N,Wang C

17-0691 was used in Flow cytometry/Cell sorting to characterise a STING-specific small-molecular inhibitor that may be applied for potentially manipulating the STING-mediated clinical diseases.

Tue Dec 18 00:00:00 EST 2018

Adipose tissue B2 cells promote insulin resistance through leukotriene LTB4/LTB4R1 signaling.

The Journal of clinical investigation

Ying W,Wollam J,Ofrecio JM,Bandyopadhyay G,El Ouarrat D,Lee YS,Oh DY,Li P,Osborn O,Olefsky JM

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2017

A type 2 cytokine axis for thymus emigration.

The Journal of experimental medicine

White AJ,Baik S,Parnell SM,Holland AM,Brombacher F,Jenkinson WE,Anderson G

17-0691 was used in Flow cytometry/Cell sorting to show the thymic microenvironment expresses the type 2 IL-4R complex and is functionally responsive to its known ligands, IL-4 and IL-13.

Mon Aug 07 00:00:00 EDT 2017

IL-36 Signaling Facilitates Activation of the NLRP3 Inflammasome and IL-23/IL-17 Axis in Renal Inflammation and Fibrosis.

Journal of the American Society of Nephrology : JASN

Chi HH,Hua KF,Lin YC,Chu CL,Hsieh CY,Hsu YJ,Ka SM,Tsai YL,Liu FC,Chen A

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Sat Jul 01 00:00:00 EDT 2017

FHL2 Regulates Natural Killer Cell Development and Activation during Streptococcus pneumoniae Infection.

Frontiers in immunology

Baranek T,Morello E,Valayer A,Aimar RF,Bréa D,Henry C,Besnard AG,Dalloneau E,Guillon A,Dequin PF,Narni-Mancinelli E,Vivier E,Laurent F,Wei Y,Paget C,Si-Tahar M

17-0691 was used in Flow cytometry/Cell sorting to determine the expression and role of FHL2 in natural killer cell biology.

Wed Nov 20 00:00:00 EST 2019

Betulin from Hedyotis hedyotidea ameliorates concanavalin A-induced and T cell-mediated autoimmune hepatitis in mice.

Acta pharmacologica Sinica

Zhou YQ,Weng XF,Dou R,Tan XS,Zhang TT,Fang JB,Wu XW

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

14-3-3 Proteins regulate K_2P 5.1 surface expression on T lymphocytes.

Traffic (Copenhagen, Denmark)

Fernández-Orth J,Ehling P,Ruck T,Pankratz S,Hofmann MS,Landgraf P,Dieterich DC,Smalla KH,Kähne T,Seebohm G,Budde T,Wiendl H,Bittner S,Meuth SG

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Sun Jan 01 00:00:00 EST 2017

Selectivity of Human TLR9 for Double CpG Motifs and Implications for the Recognition of Genomic DNA.

Journal of immunology (Baltimore, Md. : 1950)

Pohar J,Yamamoto C,Fukui R,Cajnko MM,Miyake K,Jerala R,Benčina M

17-0691 was used in Flow cytometry/Cell sorting to study the selectivity of human TLR9 for double CpG motifs.

Wed Mar 01 00:00:00 EST 2017

IRF6 Regulates Alternative Activation by Suppressing PPARγ in Male Murine Macrophages.

Endocrinology

Li C,Ying W,Huang Z,Brehm T,Morin A,Vella AT,Zhou B

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2017

Tristetraprolin inhibits macrophage IL-27-induced activation of antitumour cytotoxic T cell responses.

Nature communications

Wang Q,Ning H,Peng H,Wei L,Hou R,Hoft DF,Liu J

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Wed Oct 11 00:00:00 EDT 2017

Serum Gp96 is a chaperone of complement-C3 during graft-versus-host disease.

JCI insight

Seignez A,Joly AL,Chaumonnot K,Hazoumé A,Sanka M,Marcion G,Boudesco C,Hammann A,Seigneuric R,Jégo G,Ducoroy P,Delarue P,Senet P,Castilla-Llorente C,Solary E,Durey MA,Rubio MT,Hermine O,Kohli E,Garrido C

17-0691 was used in Flow cytometry/Cell sorting to identify glycoprotein 96 and complement C3 as partner graft-versus-host disease-induced tissue damage-associated molecular pattern molecules.

Thu Mar 23 00:00:00 EDT 2017

The efficacy of oral and subcutaneous antigen-specific immunotherapy in murine cow's milk- and peanut allergy models.

Clinical and translational allergy

Vonk MM,Wagenaar L,Pieters RHH,Knippels LMJ,Willemsen LEM,Smit JJ,van Esch BCAM,Garssen J

17-0691 was used in Flow cytometry/Cell sorting to compare oral immunotherapy and subcutaneous immunotherapy models for cows milk and peanut allergy.

Fri Apr 08 00:00:00 EDT 2022

MyD88-dependent pro-interleukin-1β induction in dendritic cells exposed to food-grade synthetic amorphous silica.

Particle and fibre toxicology

Winkler HC,Kornprobst J,Wick P,von Moos LM,Trantakis I,Schraner EM,Bathke B,Hochrein H,Suter M,Naegeli H

17-0691 was used in Flow cytometry/Cell sorting to unexpectedly show that food-grade SAS particles are able to directly initiate the endosomal MyD88-dependent pathogen pattern recognition and signaling pathway in steady-state DCs.

Fri Jun 23 00:00:00 EDT 2017

Regulatory T cell frequencies and phenotypes following anti-viral vaccination.

PloS one

de Wolf ACMT,van Aalst S,Ludwig IS,Bodinham CL,Lewis DJ,van der Zee R,van Eden W,Broere F

17-0691 was used in Flow cytometry/Cell sorting to determine the impact of anti-viral vaccination on Treg homeostasis.

Fri Sep 22 00:00:00 EDT 2017

MicroRNA-7 Deficiency Ameliorates the Pathologies of Acute Lung Injury through Elevating KLF4.

Frontiers in immunology

Zhao J,Chen C,Guo M,Tao Y,Cui P,Zhou Y,Qin N,Zheng J,Zhang J,Xu L

17-0691 was used in Flow cytometry/Cell sorting to study the role of microRNA-7 in acute lung injury, allowing the development of new therapeutic strategies against inflammatory lung disease.

Wed Mar 16 00:00:00 EDT 2022

Resident T Cells Are Unable To Control Herpes Simplex Virus-1 Activity in the Brain Ependymal Region during Latency.

Journal of immunology (Baltimore, Md. : 1950)

Menendez CM,Jinkins JK,Carr DJ

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Mon Aug 15 00:00:00 EDT 2016

Affinity and dose of TCR engagement yield proportional enhancer and gene activity in CD4+ T cells.

eLife

Allison KA,Sajti E,Collier JG,Gosselin D,Troutman TD,Stone EL,Hedrick SM,Glass CK

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Mon Jul 04 00:00:00 EDT 2016

The HSP90 inhibitor, 17AAG, protects the intestinal stem cell niche and inhibits graft versus host disease development.

Oncogene

Joly AL,Deepti A,Seignez A,Goloudina A,Hebrard S,Schmitt E,Richaud S,Fourmaux E,Hammann A,Collura A,Svrcek M,Jego G,Robinet E,Solary E,Demidov O,Kohli E,Garrido C

17-0691-82 was used in Flow cytometry/Cell sorting to investigate HSP90 inhibitors in graft versus host disease development in intestinal stem cell niches.

Thu Jun 02 00:00:00 EDT 2016

Alternative splicing of MALT1 controls signalling and activation of CD4(+) T cells.

Nature communications

Meininger I,Griesbach RA,Hu D,Gehring T,Seeholzer T,Bertossi A,Kranich J,Oeckinghaus A,Eitelhuber AC,Greczmiel U,Gewies A,Schmidt-Supprian M,Ruland J,Brocker T,Heissmeyer V,Heyd F,Krappmann D

17-0691 was used in Flow cytometry/Cell sorting to study how TCR-induced alternative splicing augments MALT1 scaffolding to enhance downstream signalling and to promote optimal T-cell activation.

Tue Apr 12 00:00:00 EDT 2016

MicroRNA-223 is a crucial mediator of PPARγ-regulated alternative macrophage activation.

The Journal of clinical investigation

Ying W,Tseng A,Chang RC,Morin A,Brehm T,Triff K,Nair V,Zhuang G,Song H,Kanameni S,Wang H,Golding MC,Bazer FW,Chapkin RS,Safe S,Zhou B

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Mon Nov 02 00:00:00 EST 2015

Single-Dose CpG Immunization Protects Against a Heterosubtypic Challenge and Generates Antigen-Specific Memory T Cells.

Frontiers in immunology

Vogel AJ,Brown DM

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Fri Jul 10 00:00:00 EDT 2015

MFG-E8 Is Critical for Embryonic Stem Cell-Mediated T Cell Immunomodulation.

Stem cell reports

Tan Y,AlKhamees B,Jia D,Li L,Couture JF,Figeys D,Jinushi M,Wang L

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Tue Nov 10 00:00:00 EST 2015

Sunitinib depletes myeloid-derived suppressor cells and synergizes with a cancer vaccine to enhance antigen-specific immune responses and tumor eradication.

Oncoimmunology

Draghiciu O,Nijman HW,Hoogeboom BN,Meijerhof T,Daemen T

17-0691 was used in Flow cytometry/Cell sorting to demonstrate that sunitinib can create a favourable microenvironment depleted of MDSCs, and act synergistically with a cancer vaccine, resulting in enhanced levels of active tumor-antigen specific CTLs.

Sun Mar 01 00:00:00 EST 2015

Immunoglobulin E plays an immunoregulatory role in lupus.

The Journal of experimental medicine

Dema B,Charles N,Pellefigues C,Ricks TK,Suzuki R,Jiang C,Scheffel J,Hasni S,Hoffman V,Jablonski M,Sacré K,Gobert D,Papo T,Daugas E,Crampton S,Bolland S,Rivera J

17-0691 was used in Flow cytometry/Cell sorting to explore the effect of IgE deficiency on the inflammatory response in FcγRIIB-deficient mice, which hastens and results in a more aggressive lupuslike disease.

Mon Oct 20 00:00:00 EDT 2014

Peptidylarginine deiminase 4 contributes to tumor necrosis factor α-induced inflammatory arthritis.

Arthritis & rheumatology (Hoboken, N.J.)

Shelef MA,Sokolove J,Lahey LJ,Wagner CA,Sackmann EK,Warner TF,Wang Y,Beebe DJ,Robinson WH,Huttenlocher A

17-0691 was used in Flow cytometry/Cell sorting to investigate whether TNFα and PAD4 may synergistically exacerbate autoantibody production and inflammatory arthritis, showing that PAD4 contributes to TNFα-induced inflammatory arthritis.

Sun Jun 01 00:00:00 EDT 2014

Mechanisms of the immunosuppressive effects of mouse adipose tissue-derived mesenchymal stromal cells on mouse alloreactively stimulated spleen cells.

Experimental and therapeutic medicine

Nagaya R,Mizuno-Kamiya M,Takayama E,Kawaki H,Onoe I,Tanabe T,Nagahara K,Kondoh N

17-0691 was used in Flow cytometry/Cell sorting to demonstrate that adipose tissue-derived mesenchymal stromal cells have significant immune modulatory effects on alloreactively stimulated spleen cells.

Wed Jan 01 00:00:00 EST 2014

Tim-3 negatively mediates natural killer cell function in LPS-induced endotoxic shock.

PloS one

Hou H,Liu W,Wu S,Lu Y,Peng J,Zhu Y,Lu Y,Wang F,Sun Z

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Mon Jul 06 00:00:00 EDT 2015

Establishment of a murine graft-versus-myeloma model using allogeneic stem cell transplantation.

PloS one

Binsfeld M,Beguin Y,Belle L,Otjacques E,Hannon M,Briquet A,Heusschen R,Drion P,Zilberberg J,Bogen B,Baron F,Caers J

17-0691 was used in Flow cytometry/Cell sorting to investigate the anti-myeloma effects of allo-SCT from B10.D2 mice into MHC-matched myeloma-bearing Balb/cJ mice.

Tue Dec 15 00:00:00 EST 2015

The thymic medulla is required for Foxp3+ regulatory but not conventional CD4+ thymocyte development.

The Journal of experimental medicine

Cowan JE,Parnell SM,Nakamura K,Caamano JH,Lane PJ,Jenkinson EJ,Jenkinson WE,Anderson G

17-0691 was used in Flow cytometry/Cell sorting to demonstrate a differential requirement for the thymic medulla in relation to CD4 conventional and Foxp3(+) thymocyte lineages.

Mon Apr 08 00:00:00 EDT 2013

Alterations in regulatory T cells induced by specific oligosaccharides improve vaccine responsiveness in mice.

PloS one

Schijf MA,Kerperien J,Bastiaans J,Szklany K,Meerding J,Hofman G,Boon L,van Wijk F,Garssen J,Van't Land B

17-0691 was used in Flow cytometry/Cell sorting to identify factors affecting the quality of the immune response to vaccination.

Tue May 27 00:00:00 EDT 2014

Angiotensin II is a new component involved in splenic T lymphocyte responses during Plasmodium berghei ANKA infection.

PloS one

Silva-Filho JL,Souza MC,Ferreira-Dasilva CT,Silva LS,Costa MF,Padua TA,Henriques Md,Morrot A,Savino W,Caruso-Neves C,Pinheiro AA

17-0691 was used in Flow cytometry/Cell sorting to provide evidence for the potential role of Ang II in regulating the splenic immune response.

Tue Nov 26 00:00:00 EST 2013

Microparticles bearing encephalitogenic peptides induce T-cell tolerance and ameliorate experimental autoimmune encephalomyelitis.

Nature biotechnology

Getts DR,Martin AJ,McCarthy DP,Terry RL,Hunter ZN,Yap WT,Getts MT,Pleiss M,Luo X,King NJ,Shea LD,Miller SD

17-0691 was used in Flow cytometry/Cell sorting to highlight the potential for using microparticles to target natural apoptotic clearance pathways to inactivate pathogenic T cells in autoimmunity.

Sat Dec 01 00:00:00 EST 2012

Dynamic in situ cytometry uncovers T cell receptor signaling during immunological synapses and kinapses in vivo.

Immunity

Moreau HD,Lemaître F,Terriac E,Azar G,Piel M,Lennon-Dumenil AM,Bousso P

17-0691 was used in Flow cytometry/Cell sorting to examine how T cells sense TCR ligands of varying affinities in vivo.

Fri Aug 24 00:00:00 EDT 2012

Anti-HIV B Cell lines as candidate vaccine biosensors.

Journal of immunology (Baltimore, Md. : 1950)

Ota T,Doyle-Cooper C,Cooper AB,Huber M,Falkowska E,Doores KJ,Hangartner L,Le K,Sok D,Jardine J,Lifson J,Wu X,Mascola JR,Poignard P,Binley JM,Chakrabarti BK,Schief WR,Wyatt RT,Burton DR,Nemazee D

17-0691 was used in Flow cytometry/Cell sorting to suggest that HIV Env antigen-expressing, infection-competent virions are poorly recognized by high affinity bNAb-expressing cells.

Thu Nov 15 00:00:00 EST 2012

Commitment to the regulatory T cell lineage requires CARMA1 in the thymus but not in the periphery.

PLoS biology

Barnes MJ,Krebs P,Harris N,Eidenschenk C,Gonzalez-Quintial R,Arnold CN,Crozat K,Sovath S,Moresco EM,Theofilopoulos AN,Beutler B,Hoebe K

17-0691 was used in Flow cytometry/Cell sorting to investigate methods to distinguish between Tregs of thymic or peripheral origin, showing that commitment requires CARMA1 in the thymus but not in the periphery.

Tue Mar 03 00:00:00 EST 2009

Immunoregulatory role of Jalpha281 T cells in aged mice developing lupus-like nephritis.

European journal of immunology

Sireci G,Russo D,Dieli F,Porcelli SA,Taniguchi M,La Manna MP,Di Liberto D,Scarpa F,Salerno A

17-0691 was used in Flow cytometry/Cell sorting to study renal functions and autoantibody production in aged Ja281 KO mice lacking iNKT cells.

Thu Feb 01 00:00:00 EST 2007

Modulation of naive CD4+ T-cell responses to an airway antigen during pulmonary mycobacterial infection.

Infection and immunity

Anis MM,Fulton SA,Reba SM,Harding CV,Boom WH

Published figure using CD69 monoclonal antibody (Product # 17-0691-82) in Flow Cytometry

Tue May 01 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: CD69; Early activation antigen CD69; Very Early Activation Antigen

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Gene Aliases: 5830438K24Rik; AI452015; AIM; Cd69; VEA

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UniProt ID: (Mouse) P37217

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Entrez Gene ID: (Mouse) 12515

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Function(s)

calcium ion binding carbohydrate binding

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CD69 Monoclonal Antibody (H1.2F3), APC, eBioscience™

CD69 Antibody (17-0691-82) in Flow

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17-0691-82

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