Invitrogen

CD69 Monoclonal Antibody (H1.2F3), PerCP-Cyanine5.5, eBioscience™

FIGURE: 1 / 68

CD69 Antibody (45-0691-82) in Flow

Staining of 1-day ConA-stimulated BALB/c splenocytes with 0.25 µg of Armenian Hamster IgG Isotype Control PerCP-Cyanine5-5 (Product # 45-4888-80) (open histogram) or 0.25 µg of Anti-Mouse CD69 PerCP-Cyanine5-5 (filled histogram). Total viable cells were used for analysis.

Product Image Gallery

Product Details

45-0691-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 67 publications 67 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), PerCP-Cyanine5.5, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

H1.2F3

Conjugate

PerCP-Cyanine5.5 PerCP-Cyanine5.5 PerCP-Cyanine5.5

Excitation/Emission Max

489/679 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1210703

Product Specific Information

Description: The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen (VEA). CD69 is approximately 35 kDa and is expressed on the surface as a disulfide-linked dimer. While a small subset of lymphocytes in the thymus, spleen and lymph nodes express this antigen, activation of both T and B cells rapidly upregulates the surface expression of CD69, suggesting a role for CD69 in lymphocyte development and activation.

Applications Reported: This H1.2F3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This H1.2F3 antibody has been tested by flow cytometric analysis of activated splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide bonded homodimer with a molecular weight of 60 kDa under non-reducing conditions. CD69 contains one or two N linked oligosacaride and the molecule is present on activated platelets. In normal peripheral blood a variable percentage of cells express the CD69 antigen, and it is involved in lymphocyte signal transduction. Expression CD69 is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. Alternative splicing results in multiple transcript variants of CD69. Diseases associated with CD69 dysfunction include coccidiodomycosis and asthma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

HSF1 promotes CD69⁺ Treg differentiation to inhibit colitis progression.

Theranostics

Yu L,Zhou B,Zhu Y,Li L,Zhong Y,Zhu L,Wang H,Chen H,Xu J,Guo T,Feng L,Wang X,Cai Z,Wang J,Jin H

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Tue Apr 18 00:00:00 EDT 2023

Novel Bruton's tyrosine kinase inhibitor TAS5315 suppresses the progression of inflammation and joint destruction in rodent collagen-induced arthritis.

PloS one

Akasaka D,Iguchi S,Kaneko R,Yoshiga Y,Kajiwara D,Nakachi Y,Noma N,Tanaka K,Shimizu A,Hosoi F

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Mon Feb 27 00:00:00 EST 2023

Intranasal Immunization With a c-di-GMP-Adjuvanted Acellular Pertussis Vaccine Provides Superior Immunity Against Bordetella pertussis in a Mouse Model.

Frontiers in immunology

Jiang W,Wang X,Su Y,Cai L,Li J,Liang J,Gu Q,Sun M,Shi L

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Tue May 03 00:00:00 EDT 2022

Meningeal lymphatic drainage promotes T cell responses against Toxoplasma gondii but is dispensable for parasite control in the brain.

eLife

Kovacs MA,Cowan MN,Babcock IW,Sibley LA,Still K,Batista SJ,Labuzan SA,Sethi I,Harris TH

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Wed Dec 21 00:00:00 EST 2022

An autonomous TCR signal-sensing switch influences CD4/CD8 lineage choice in mice.

Communications biology

Basu J,Zha J,Nicolas E,Coulton M,Czyzewicz P,Hua X,Ge L,Kappes DJ

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Fri Jan 21 00:00:00 EST 2022

CP-25 exerts a protective effect against ConA-induced hepatitis via regulating inflammation and immune response.

Frontiers in pharmacology

Li N,Wu JJ,Qi M,Wang ZY,Zhang SN,Li XQ,Chen TT,Wang MF,Zhang LL,Wei W,Sun WY

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Sat Dec 03 00:00:00 EST 2022

A novel costimulatory molecule gene-modified leukemia cell-derived exosome-targeted CD4⁺ T cell vaccine efficiently enhances anti-leukemia immunity.

Frontiers in immunology

Li J,Huang F,Jiang Y,Zhao J,Wan J,Hao S

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Tue Dec 06 00:00:00 EST 2022

cDC1 coordinate innate and adaptive responses in the omentum required for T cell priming and memory.

Science immunology

Christian DA,Adams TA,Shallberg LA,Phan AT,Smith TE,Abraha M,Perry J,Ruthel G,Clark JT,Pritchard GH,Aronson LR,Gossa S,McGavern DB,Kedl RM,Hunter CA

45-0691-82 was used in Flow Cytometry to investigate how conventional type 1 dendritic cells (cDC1s) in the peritoneal cavity's omentum coordinate innate responses to microbial challenge and provide secondary signals required for CD8+ T cell expansion and memory formation, particularly after intraperitoneal immunization with Toxoplasma gondii.

Fri Sep 30 00:00:00 EDT 2022

ADAM12 is a costimulatory molecule that determines Th1 cell fate and mediates tissue inflammation.

Cellular & molecular immunology

Liu Y,Bockermann R,Hadi M,Safari I,Carrion B,Kveiborg M,Issazadeh-Navikas S

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Sun Aug 01 00:00:00 EDT 2021

Uncovering a novel role of PLCβ4 in selectively mediating TCR signaling in CD8+ but not CD4+ T cells.

The Journal of experimental medicine

Sasai M,Ma JS,Okamoto M,Nishino K,Nagaoka H,Takashima E,Pradipta A,Lee Y,Kosako H,Suh PG,Yamamoto M

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Mon Jul 05 00:00:00 EDT 2021

Fasudil prevents liver fibrosis via activating natural killer cells and suppressing hepatic stellate cells.

World journal of gastroenterology

Han QJ,Mu YL,Zhao HJ,Zhao RR,Guo QJ,Su YH,Zhang J

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Mon Jun 28 00:00:00 EDT 2021

TRPM8 channel augments T-cell activation and proliferation.

Cell biology international

Acharya TK,Tiwari A,Majhi RK,Goswami C

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Cell-permeable transgelin-2 as a potent therapeutic for dendritic cell-based cancer immunotherapy.

Journal of hematology & oncology

Kim HR,Park JS,Park JH,Yasmin F,Kim CH,Oh SK,Chung IJ,Jun CD

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Wed Mar 17 00:00:00 EDT 2021

Suppression of mucosal Th17 memory responses by acellular pertussis vaccines enhances nasal Bordetella pertussis carriage.

NPJ vaccines

Dubois V,Chatagnon J,Thiriard A,Bauderlique-Le Roy H,Debrie AS,Coutte L,Locht C

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Fri Jan 08 00:00:00 EST 2021

STING Activated Tumor-Intrinsic Type I Interferon Signaling Promotes CXCR3 Dependent Antitumor Immunity in Pancreatic Cancer.

Cellular and molecular gastroenterology and hepatology

Vonderhaar EP,Barnekow NS,McAllister D,McOlash L,Eid MA,Riese MJ,Tarakanova VL,Johnson BD,Dwinell MB

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Mon Jan 24 00:00:00 EST 2022

GPR120 induces regulatory dendritic cells by inhibiting HK2-dependent glycolysis to alleviate fulminant hepatic failure.

Cell death & disease

Yu H,Yang W,Huang J,Miao X,Wang B,Ren X,Gu Y,Wang Q,Ding X,Guo X,Qian F,Zhang Y,Xu H,Zheng L,Jin M

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Thu Dec 16 00:00:00 EST 2021

Robust induction of T_RMs by combinatorial nanoshells confers cross-strain sterilizing immunity against lethal influenza viruses.

Molecular therapy. Methods & clinical development

Lin PH,Liang CY,Yao BY,Chen HW,Pan CF,Wu LL,Lin YH,Hsu YS,Liu YH,Chen PJ,Hu CJ,Yang HC

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Fri Jun 11 00:00:00 EDT 2021

SPARC regulation of PMN clearance protects from pristane-induced lupus and rheumatoid arthritis.

iScience

Sangaletti S,Botti L,Gulino A,Lecis D,Bassani B,Portararo P,Milani M,Cancila V,De Cecco L,Dugo M,Tripodo C,Colombo MP

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Fri Jun 25 00:00:00 EDT 2021

ILC3s control airway inflammation by limiting T cell responses to allergens and microbes.

Cell reports

Teng F,Tachó-Piñot R,Sung B,Farber DL,Worgall S,Hammad H,Lambrecht BN,Hepworth MR,Sonnenberg GF

45-0691-82 was used in Flow cytometry/Cell sorting to demonstrate a critical role for antigen-presenting ILC3s in orchestrating immune tolerance in the airway by restricting pro-inflammatory T cell responses to both allergens and microbes.

Tue Nov 23 00:00:00 EST 2021

Hybrid Gene Origination Creates Human-Virus Chimeric Proteins during Infection.

Cell

Ho JSY,Angel M,Ma Y,Sloan E,Wang G,Martinez-Romero C,Alenquer M,Roudko V,Chung L,Zheng S,Chang M,Fstkchyan Y,Clohisey S,Dinan AM,Gibbs J,Gifford R,Shen R,Gu Q,Irigoyen N,Campisi L,Huang C,Zhao N,Jones JD,van Knippenberg I,Zhu Z,Moshkina N,Meyer L,Noel J,Peralta Z,Rezelj V,Kaake R,Rosenberg B,Wang B,Wei J,Paessler S,Wise HM,Johnson J,Vannini A,Amorim MJ,Baillie JK,Miraldi ER,Benner C,Brierley I,Digard P,Łuksza M,Firth AE,Krogan N,Greenbaum BD,MacLeod MK,van Bakel H,Garcìa-Sastre A,Yewdell JW,Hutchinson E,Marazzi I

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Thu Jun 25 00:00:00 EDT 2020

An Integrated Epigenomic and Transcriptomic Map of Mouse and Human αβ T Cell Development.

Immunity

Chopp LB,Gopalan V,Ciucci T,Ruchinskas A,Rae Z,Lagarde M,Gao Y,Li C,Bosticardo M,Pala F,Livak F,Kelly MC,Hannenhalli S,Bosselut R

45-0691-82 was used in Flow Cytometry to identify mouse and human αβ thymocyte developmental trajectories.

Tue Dec 15 00:00:00 EST 2020

Contact-dependent delivery of IL-2 by dendritic cells to CD4 T cells in the contraction phase promotes their long-term survival.

Protein & cell

Tong D,Zhang L,Ning F,Xu Y,Hu X,Shi Y

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Sat Feb 01 00:00:00 EST 2020

Increase in liver γδ T cells with concurrent augmentation of IFN-β production during the early stages of a mouse model of acute experimental hepatitis B virus infection.

Experimental and therapeutic medicine

Chang L,Wang L,Ling N,Peng H,Chen M

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2020

Effects of CD4⁺ T lymphocytes from ovariectomized mice on bone marrow mesenchymal stem cell proliferation and osteogenic differentiation.

Experimental and therapeutic medicine

Shao BY,Wang L,Yu Y,Chen L,Gan N,Huang WM

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2020

Potentiating the Antitumor Activity of Cytotoxic T Cells via the Transmembrane Domain of IGSF4 That Increases TCR Avidity.

Frontiers in immunology

Kim HR,Park JS,Fatima Y,Kausar M,Park JH,Jun CD

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Tue Jul 27 00:00:00 EDT 2021

Targeting the orphan nuclear receptor NR2F6 in T cells primes tumors for immune checkpoint therapy.

Cell communication and signaling : CCS

Klepsch V,Pommermayr M,Humer D,Brigo N,Hermann-Kleiter N,Baier G

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Tue Jan 14 00:00:00 EST 2020

SLAMF6 deficiency augments tumor killing and skews toward an effector phenotype revealing it as a novel T cell checkpoint.

eLife

Hajaj E,Eisenberg G,Klein S,Frankenburg S,Merims S,Ben David I,Eisenhaure T,Henrickson SE,Villani AC,Hacohen N,Abudi N,Abramovich R,Cohen JE,Peretz T,Veillette A,Lotem M

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Tue Mar 03 00:00:00 EST 2020

Loss of testosterone impairs anti-tumor neutrophil function.

Nature communications

Markman JL,Porritt RA,Wakita D,Lane ME,Martinon D,Noval Rivas M,Luu M,Posadas EM,Crother TR,Arditi M

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Tue Mar 31 00:00:00 EDT 2020

NPM1 upregulates the transcription of PD-L1 and suppresses T cell activity in triple-negative breast cancer.

Nature communications

Qin G,Wang X,Ye S,Li Y,Chen M,Wang S,Qin T,Zhang C,Li Y,Long Q,Hu H,Shi D,Li J,Zhang K,Zhai Q,Tang Y,Kang T,Lan P,Xie F,Lu J,Deng W

45-0691-82 was used in Flow Cytometry to verify that triple-negative breast cancer (TNBC) has higher PD-L1 expression than other subtypes.

Fri Apr 03 00:00:00 EDT 2020

CSF1R antagonism limits local restimulation of antiviral CD8⁺ T cells during viral encephalitis.

Journal of neuroinflammation

Funk KE,Klein RS

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Thu Jan 31 00:00:00 EST 2019

Enhanced germinal center reaction by targeting vaccine antigen to major histocompatibility complex class II molecules.

NPJ vaccines

Andersen TK,Huszthy PC,Gopalakrishnan RP,Jacobsen JT,Fauskanger M,Tveita AA,Grødeland G,Bogen B

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Thu Oct 05 00:00:00 EDT 2023

The Inhibitory T Cell Receptors PD1 and 2B4 Are Differentially Regulated on CD4 and CD8 T Cells in a Mouse Model of Non-alcoholic Steatohepatitis.

Frontiers in pharmacology

Hansel C,Erschfeld S,Baues M,Lammers T,Weiskirchen R,Trautwein C,Kroy DC,Drescher HK

45-0691 was used in Flow cytometry/Cell sorting to investigate the expression profile of inhibitory T cell receptors on CD4 and CD8 T cells in a mouse model of non-alcoholic steatohepatitis.

Wed Sep 30 00:00:00 EDT 2020

Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells.

Cell

Gaya M,Barral P,Burbage M,Aggarwal S,Montaner B,Warren Navia A,Aid M,Tsui C,Maldonado P,Nair U,Ghneim K,Fallon PG,Sekaly RP,Barouch DH,Shalek AK,Bruckbauer A,Strid J,Batista FD

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Thu Jan 25 00:00:00 EST 2018

A Pck1-directed glycogen metabolic program regulates formation and maintenance of memory CD8⁺ T cells.

Nature cell biology

Ma R,Ji T,Zhang H,Dong W,Chen X,Xu P,Chen D,Liang X,Yin X,Liu Y,Ma J,Tang K,Zhang Y,Peng Y,Lu J,Zhang Y,Qin X,Cao X,Wan Y,Huang B

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Mon Jan 01 00:00:00 EST 2018

Intranasal delivery of adjuvant-free peptide nanofibers elicits resident CD8⁺ T cell responses.

Journal of controlled release : official journal of the Controlled Release Society

Si Y,Wen Y,Kelly SH,Chong AS,Collier JH

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Sat Jul 28 00:00:00 EDT 2018

MicroRNA-126 deficiency enhanced the activation and function of CD4⁺ T cells by elevating IRS-1 pathway.

Clinical and experimental immunology

Chu F,Hu Y,Zhou Y,Guo M,Lu J,Zheng W,Xu H,Zhao J,Xu L

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Thu Feb 01 00:00:00 EST 2018

Oncofetal gene SALL4 reactivation by hepatitis B virus counteracts miR-200c in PD-L1-induced T cell exhaustion.

Nature communications

Sun C,Lan P,Han Q,Huang M,Zhang Z,Xu G,Song J,Wang J,Wei H,Zhang J,Sun R,Zhang C,Tian Z

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Wed Mar 28 00:00:00 EDT 2018

MAIT cells contribute to protection against lethal influenza infection in vivo.

Nature communications

van Wilgenburg B,Loh L,Chen Z,Pediongco TJ,Wang H,Shi M,Zhao Z,Koutsakos M,Nüssing S,Sant S,Wang Z,D'Souza C,Jia X,Almeida CF,Kostenko L,Eckle SBG,Meehan BS,Kallies A,Godfrey DI,Reading PC,Corbett AJ,McCluskey J,Klenerman P,Kedzierska K,Hinks TSC

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Fri Nov 09 00:00:00 EST 2018

Homoharringtonine induced immune alteration for an Efficient Anti-tumor Response in Mouse Models of Non-small Cell Lung Adenocarcinoma Expressing Kras Mutation.

Scientific reports

Weng TY,Wu HF,Li CY,Hung YH,Chang YW,Chen YL,Hsu HP,Chen YH,Wang CY,Chang JY,Lai MD

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Tue May 29 00:00:00 EDT 2018

Development of a screening strategy for new modulators of T cell receptor signaling and T cell activation.

Scientific reports

Chen EW,Brzostek J,Gascoigne NRJ,Rybakin V

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Tue Jul 03 00:00:00 EDT 2018

Adipose tissue B2 cells promote insulin resistance through leukotriene LTB4/LTB4R1 signaling.

The Journal of clinical investigation

Ying W,Wollam J,Ofrecio JM,Bandyopadhyay G,El Ouarrat D,Lee YS,Oh DY,Li P,Osborn O,Olefsky JM

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2017

IL-36 Signaling Facilitates Activation of the NLRP3 Inflammasome and IL-23/IL-17 Axis in Renal Inflammation and Fibrosis.

Journal of the American Society of Nephrology : JASN

Chi HH,Hua KF,Lin YC,Chu CL,Hsieh CY,Hsu YJ,Ka SM,Tsai YL,Liu FC,Chen A

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Sat Jul 01 00:00:00 EDT 2017

Betulin from Hedyotis hedyotidea ameliorates concanavalin A-induced and T cell-mediated autoimmune hepatitis in mice.

Acta pharmacologica Sinica

Zhou YQ,Weng XF,Dou R,Tan XS,Zhang TT,Fang JB,Wu XW

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

14-3-3 Proteins regulate K_2P 5.1 surface expression on T lymphocytes.

Traffic (Copenhagen, Denmark)

Fernández-Orth J,Ehling P,Ruck T,Pankratz S,Hofmann MS,Landgraf P,Dieterich DC,Smalla KH,Kähne T,Seebohm G,Budde T,Wiendl H,Bittner S,Meuth SG

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Sun Jan 01 00:00:00 EST 2017

IRF6 Regulates Alternative Activation by Suppressing PPARγ in Male Murine Macrophages.

Endocrinology

Li C,Ying W,Huang Z,Brehm T,Morin A,Vella AT,Zhou B

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2017

Tristetraprolin inhibits macrophage IL-27-induced activation of antitumour cytotoxic T cell responses.

Nature communications

Wang Q,Ning H,Peng H,Wei L,Hou R,Hoft DF,Liu J

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Wed Oct 11 00:00:00 EDT 2017

Metabolic maintenance of cell asymmetry following division in activated T lymphocytes.

Nature

Verbist KC,Guy CS,Milasta S,Liedmann S,Kamiński MM,Wang R,Green DR

45-0691 was used in Flow cytometry/Cell sorting to investigate the mechanisms that sustain differential cell fates in daughter T cells after activation by an antigen-presenting cell.

Thu Apr 21 00:00:00 EDT 2016

Resident T Cells Are Unable To Control Herpes Simplex Virus-1 Activity in the Brain Ependymal Region during Latency.

Journal of immunology (Baltimore, Md. : 1950)

Menendez CM,Jinkins JK,Carr DJ

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Mon Aug 15 00:00:00 EDT 2016

Affinity and dose of TCR engagement yield proportional enhancer and gene activity in CD4+ T cells.

eLife

Allison KA,Sajti E,Collier JG,Gosselin D,Troutman TD,Stone EL,Hedrick SM,Glass CK

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Mon Jul 04 00:00:00 EDT 2016

miR-150 regulates obesity-associated insulin resistance by controlling B cell functions.

Scientific reports

Ying W,Tseng A,Chang RC,Wang H,Lin YL,Kanameni S,Brehm T,Morin A,Jones B,Splawn T,Criscitiello M,Golding MC,Bazer FW,Safe S,Zhou B

45-0691 was used in Flow cytometry/Cell sorting to demonstrate a role for miR-150 in regulating B cell functions in adipose tissue.

Mon Feb 01 00:00:00 EST 2016

MicroRNA-223 is a crucial mediator of PPARγ-regulated alternative macrophage activation.

The Journal of clinical investigation

Ying W,Tseng A,Chang RC,Morin A,Brehm T,Triff K,Nair V,Zhuang G,Song H,Kanameni S,Wang H,Golding MC,Bazer FW,Chapkin RS,Safe S,Zhou B

45-0691 was used in Flow cytometry/Cell sorting to investigate the regulatory network of polarised activation of adipose tissue macrophages.

Mon Nov 02 00:00:00 EST 2015

Single-Dose CpG Immunization Protects Against a Heterosubtypic Challenge and Generates Antigen-Specific Memory T Cells.

Frontiers in immunology

Vogel AJ,Brown DM

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Fri Jul 10 00:00:00 EDT 2015

Diverse roles for T-bet in the effector responses required for resistance to infection.

Journal of immunology (Baltimore, Md. : 1950)

Harms Pritchard G,Hall AO,Christian DA,Wagage S,Fang Q,Muallem G,John B,Glatman Zaretsky A,Dunn WG,Perrigoue J,Reiner SL,Hunter CA

45-0691 was used in Flow cytometry/Cell sorting to highlight T-bet-independent pathways to IFN-γ production and reveal a novel role for this transcription factor in coordinating the T cell responses necessary to control infection in peripheral tissues.

Sun Feb 01 00:00:00 EST 2015

MFG-E8 Is Critical for Embryonic Stem Cell-Mediated T Cell Immunomodulation.

Stem cell reports

Tan Y,AlKhamees B,Jia D,Li L,Couture JF,Figeys D,Jinushi M,Wang L

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Tue Nov 10 00:00:00 EST 2015

Sunitinib depletes myeloid-derived suppressor cells and synergizes with a cancer vaccine to enhance antigen-specific immune responses and tumor eradication.

Oncoimmunology

Draghiciu O,Nijman HW,Hoogeboom BN,Meijerhof T,Daemen T

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Sun Mar 01 00:00:00 EST 2015

H9N2 influenza whole inactivated virus combined with polyethyleneimine strongly enhances mucosal and systemic immunity after intranasal immunization in mice.

Clinical and vaccine immunology : CVI

Qin T,Yin Y,Huang L,Yu Q,Yang Q

45-0691 was used in Flow cytometry/Cell sorting to investigate the immune activity of polyethyleneimine with H9N2 influenza whole inactivated virus, showing that it enhances mucosal and systemic immunity after intranasal immunization.

Wed Apr 01 00:00:00 EDT 2015

Quantitative and temporal requirements revealed for Zap70 catalytic activity during T cell development.

Nature immunology

Au-Yeung BB,Melichar HJ,Ross JO,Cheng DA,Zikherman J,Shokat KM,Robey EA,Weiss A

45-0691 was used in Flow cytometry/Cell sorting to show that CD4(+)CD8(+) thymocytes integrate multiple, transient, Zap70-dependent signals over more than 36 h to reach a cumulative threshold for positive selection.

Tue Jul 01 00:00:00 EDT 2014

mTORC1-dependent metabolic reprogramming is a prerequisite for NK cell effector function.

Journal of immunology (Baltimore, Md. : 1950)

Donnelly RP,Loftus RM,Keating SE,Liou KT,Biron CA,Gardiner CM,Finlay DK

45-0691 was used in Flow cytometry/Cell sorting to investigate whether mTORC1-controlled metabolism underpins normal natural killer cell proinflammatory functions.

Sat Nov 01 00:00:00 EDT 2014

Differential requirement for CCR4 and CCR7 during the development of innate and adaptive αβT cells in the adult thymus.

Journal of immunology (Baltimore, Md. : 1950)

Cowan JE,McCarthy NI,Parnell SM,White AJ,Bacon A,Serge A,Irla M,Lane PJ,Jenkinson EJ,Jenkinson WE,Anderson G

45-0691 was used in Flow cytometry/Cell sorting to identify novel roles for CCR7 during intrathymic T cell development, highlighting its importance in enabling multiple αβT cell lineages to access the thymic medulla.

Fri Aug 01 00:00:00 EDT 2014

Tim-3 negatively mediates natural killer cell function in LPS-induced endotoxic shock.

PloS one

Hou H,Liu W,Wu S,Lu Y,Peng J,Zhu Y,Lu Y,Wang F,Sun Z

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Mon Jul 06 00:00:00 EDT 2015

The thymic medulla is required for Foxp3+ regulatory but not conventional CD4+ thymocyte development.

The Journal of experimental medicine

Cowan JE,Parnell SM,Nakamura K,Caamano JH,Lane PJ,Jenkinson EJ,Jenkinson WE,Anderson G

45-0691 was used in Flow cytometry/Cell sorting to demonstrate a differential requirement for the thymic medulla in relation to CD4 conventional and Foxp3(+) thymocyte lineages.

Mon Apr 08 00:00:00 EDT 2013

Gr1(int)CD11b+ myeloid-derived suppressor cells in Mycobacterium tuberculosis infection.

PloS one

Obregón-Henao A,Henao-Tamayo M,Orme IM,Ordway DJ

45-0691 was used in Immunofluorescence to compare the bacterial burden, lung pathology and Gr1(int)CD11b(+) myeloid-derived suppressor cell immune responses in M. tuberculosis infected mice.

Fri Jun 20 00:00:00 EDT 2014

Developmentally regulated availability of RANKL and CD40 ligand reveals distinct mechanisms of fetal and adult cross-talk in the thymus medulla.

Journal of immunology (Baltimore, Md. : 1950)

Desanti GE,Cowan JE,Baik S,Parnell SM,White AJ,Penninger JM,Lane PJ,Jenkinson EJ,Jenkinson WE,Anderson G

45-0691 was used in Flow cytometry/Cell sorting to study how multiple hemopoietic cell types regulate mTEC development through differential provision of RANKL/CD40L during ontogeny, revealing molecular differences in foetal and adult hemopoietic cross-talk.

Sat Dec 15 00:00:00 EST 2012

The acute environment, rather than T cell subset pre-commitment, regulates expression of the human T cell cytokine amphiregulin.

PloS one

Qi Y,Operario DJ,Georas SN,Mosmann TR

45-0691 was used in Flow cytometry/Cell sorting to collate a detailed analysis of the regulation of Amphiregulin expression by human T cell subsets.

Thu Dec 13 00:00:00 EST 2012

Combination treatment of mice with CRx-153 (nortriptyline and desloratadine) decreases the severity of experimental autoimmune encephalomyelitis.

Cellular immunology

Podojil JR,Padval MV,Miller SD

45-0691 was used in Flow cytometry/Cell sorting to determine if treatment of PLP(139-151)-induced R-EAE with a combination of two drugs decreases R-EAE disease.

Fri Oct 14 00:00:00 EDT 2011

Partial restoration of T-cell function in aged mice by in vitro blockade of the PD-1/ PD-L1 pathway.

Aging cell

Lages CS,Lewkowich I,Sproles A,Wills-Karp M,Chougnet C

45-0691 was used in Flow cytometry/Cell sorting to suggest that blockade of the PD-1/PD-L1 is not likely to be efficient at restoring exhausted T-cell responses in aged hosts.

Fri Oct 01 00:00:00 EDT 2010

Modulation of naive CD4+ T-cell responses to an airway antigen during pulmonary mycobacterial infection.

Infection and immunity

Anis MM,Fulton SA,Reba SM,Harding CV,Boom WH

Published figure using CD69 monoclonal antibody (Product # 45-0691-82) in Flow Cytometry

Tue May 01 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: CD69; Early activation antigen CD69; Very Early Activation Antigen

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Gene Aliases: 5830438K24Rik; AI452015; AIM; Cd69; VEA

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UniProt ID: (Mouse) P37217

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Entrez Gene ID: (Mouse) 12515

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Function(s)

calcium ion binding carbohydrate binding

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CD69 Monoclonal Antibody (H1.2F3), PerCP-Cyanine5.5, eBioscience™

CD69 Antibody (45-0691-82) in Flow

Product Details

45-0691-82

Flow Cytometry (Flow)

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