Invitrogen

Arginase 1 Monoclonal Antibody (A1exF5), APC, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

16 Published Figures

23 References

View all (94) Arginase 1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Arginase 1 Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 18

Arginase 1 Antibody (17-3697-82) in Flow

Normal human lysed whole blood cells were stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with 0.5 µg of Arginase 1 Monoclonal Antibody, APC. Cells were co-stained with CD16 Monoclonal Antibody, FITC (Product # 11-0168-42) and CD193 Monoclonal Antibody, PE (Product # 12-1939-42). Cells in the granulocyte gate (left panel) were used for analysis and were gated on either the CD193+ population (blue histogram) or the CD16+ population (purple histogram).

Arginase 1 Antibody in Immunohistochemistry (IHC)

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Product Details

17-3697-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

1.0 µg/test

View 22 publications 22 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

A1exF5

Immunogen

E.coli-derived recombinant mouse Arginase 1

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2734835

Product Specific Information

Description: The monoclonal antibody A1exF5 recognizes both human and mouse Arginase 1, a cytosolic enzyme (Arg1). This A1exF5 clone is compatible with both the standard intracellular protocols and the Foxp3/Transcription Factor Staining Buffer Set.

Applications Reported: This A1exF5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This A1exF5 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Target Information

Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, which is the final step in the urea cycle. The resulting polyamines are important for cell proliferation and removal of toxins that arise from protein degradation. By degrading arginine, Arginase 1 deprives NO synthase of its substrate and down-regulates nitric oxide production. In both human and mouse, Arginase 1 is expressed in the liver, neutrophils, myeloid derived suppressor cells (MDSC) and neural stem cells. In human, expression in blood neutrophils but not in CCR3+ granulocytes has been reported. In mice, expression of Arginase 1 is one of the hallmarks of alternatively activated macrophages (M2a). Arginase-1 may be expressed in the myeloid cells infiltrating tumors, and is typically found in the majority of hepatocellular carcinomas. Defects in Arginase 1 are the cause of argininemia, an autosomal recessive disorder characterized by hyperammonemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Advanced phosphocreatine-grafted chitosan hydrogel promote wound healing by macrophage modulation.

Frontiers in bioengineering and biotechnology

Sheng W,Qin H,Wang T,Zhao J,Fang C,Zhang P,Liu P,Udduttula A,Zeng H,Chen Y

Published figure using Arginase 1 monoclonal antibody (Product # 17-3697-82) in Flow Cytometry

Wed May 31 00:00:00 EDT 2023

Timing of Interleukin-4 Stimulation of Macrophages Determines Their Anti-Microbial Activity during Infection with Salmonella enterica Serovar Typhimurium.

Cells

Brigo N,Neumaier E,Pfeifhofer-Obermair C,Grubwieser P,Engl S,Berger S,Seifert M,Reinstadler V,Oberacher H,Weiss G

Published figure using Arginase 1 monoclonal antibody (Product # 17-3697-82) in Flow Cytometry

Fri Apr 14 00:00:00 EDT 2023

Timing of Interleukin-4 Stimulation of Macrophages Determines Their Anti-Microbial Activity during Infection with Salmonella enterica Serovar Typhimurium.

Cells

Brigo N,Neumaier E,Pfeifhofer-Obermair C,Grubwieser P,Engl S,Berger S,Seifert M,Reinstadler V,Oberacher H,Weiss G

Published figure using Arginase 1 monoclonal antibody (Product # 17-3697-82) in Flow Cytometry

Fri Apr 14 00:00:00 EDT 2023

Critical contributions of protein cargos to the functions of macrophage-derived extracellular vesicles.

Journal of nanobiotechnology

Liu B,Nguyen PL,Yu H,Li X,Wang H,Price J,Niu M,Guda C,Cheng X,Sun X,Moreau R,Ramer-Tait A,Naldrett MJ,Alvarez S,Yu J

Published figure using Arginase 1 monoclonal antibody (Product # 17-3697-82) in Flow Cytometry

Thu Sep 28 00:00:00 EDT 2023

Repurposing nitric oxide donating drugs in cancer therapy through immune modulation.

Journal of experimental & clinical cancer research : CR

Li CY,Anuraga G,Chang CP,Weng TY,Hsu HP,Ta HDK,Su PF,Chiu PH,Yang SJ,Chen FW,Ye PH,Wang CY,Lai MD

Published figure using Arginase 1 monoclonal antibody (Product # 17-3697-82) in Flow Cytometry

Sat Jan 14 00:00:00 EST 2023

Calcaratarin D, a labdane diterpenoid, attenuates mouse asthma via modulating alveolar macrophage function.

British journal of pharmacology

Liao W,Foo HYC,Tran TNQ,Chai CLL,Wong WSF

Published figure using Arginase 1 monoclonal antibody (Product # 17-3697-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2023

Curcumin-dependent phenotypic transformation of microglia mediates resistance to pseudorabies-induced encephalitis.

Veterinary research

Feng L,Luo G,Li Y,Zhang C,Liu Y,Liu Y,Chen H,He D,Zhu Y,Gan L

Published figure using Arginase 1 monoclonal antibody (Product # 17-3697-82) in Flow Cytometry

Tue Mar 14 00:00:00 EDT 2023

Establishment and Characterization of Free-Floating 3D Macrophage Programming Model in the Presence of Cancer Cell Spheroids.

International journal of molecular sciences

Korotkaja K,Jansons J,Spunde K,Rudevica Z,Zajakina A

17-3697-82 was used in Flow cytometry/Cell sorting to establish and characterized a new in vitro 3D model for macrophage programming in the presence of cancer cell spheroids.

Wed Jun 28 00:00:00 EDT 2023

Interleukin-10 genetically modified clinical-grade mesenchymal stromal cells markedly reinforced functional recovery after spinal cord injury via directing alternative activation of macrophages.

Cellular & molecular biology letters

Gao T,Huang F,Wang W,Xie Y,Wang B

Published figure using Arginase 1 monoclonal antibody (Product # 17-3697-82) in Flow Cytometry

Thu Mar 17 00:00:00 EDT 2022

Angiotensin 1-7 and its analogue decrease blood pressure but aggravate renal damage in preeclamptic mice.

Experimental animals

Liu Y,Zhai R,Tong J,Yu Y,Yang L,Gu Y,Niu J

Published figure using Arginase 1 monoclonal antibody (Product # 17-3697-82) in Flow Cytometry

Thu Nov 10 00:00:00 EST 2022

LINC00240/miR-155 axis regulates function of trophoblasts and M2 macrophage polarization via modulating oxidative stress-induced pyroptosis in preeclampsia.

Molecular medicine (Cambridge, Mass.)

Wu HY,Liu K,Zhang JL

Published figure using Arginase 1 monoclonal antibody (Product # 17-3697-82) in Flow Cytometry

Sat Sep 24 00:00:00 EDT 2022

Gene-selective transcription promotes the inhibition of tissue reparative macrophages by TNF.

Life science alliance

Dichtl S,Sanin DE,Koss CK,Willenborg S,Petzold A,Tanzer MC,Dahl A,Kabat AM,Lindenthal L,Zeitler L,Satzinger S,Strasser A,Mann M,Roers A,Eming SA,El Kasmi KC,Pearce EJ,Murray PJ

Published figure using Arginase 1 monoclonal antibody (Product # 17-3697-82) in Flow Cytometry

Fri Apr 01 00:00:00 EDT 2022

A Mouse Infection Model with a Wildtype Salmonella enterica Serovar Typhimurium Strain for the Analysis of Inflammatory Innate Immune Cells.

Bio-protocol

Pfeifhofer-Obermair C,Brigo N,Tymoszuk P,Weiss AG

17-3697-82 was used in Flow cytometry/Cell sorting to establish a robust experimental model for the characterization of these cell types and their response during host-pathogen interactions.

Tue Apr 05 00:00:00 EDT 2022

Flow Cytometric Characterization of Macrophages Infected in vitro with Salmonella enterica Serovar Typhimurium Expressing Red Fluorescent Protein.

Bio-protocol

Brigo N,Pfeifhofer-Obermair C,Demetz E,Tymoszuk P,Weiss G

17-3697-82 was used in Flow cytometry/Cell sorting to suggest a method that identifies pro- and anti-inflammatory bone marrow-derived macrophage subtypes, during an infection with Salmonella enterica serovar Typhimurium.

Sun Jun 05 00:00:00 EDT 2022

Lactate and IL6 define separable paths of inflammatory metabolic adaptation.

Science advances

Dichtl S,Lindenthal L,Zeitler L,Behnke K,Schlösser D,Strobl B,Scheller J,El Kasmi KC,Murray PJ

17-3697-82 was used in Flow Cytometry to test the interrelationships between histone lactylation (Kla) and tissue reparative gene expression.

Tue Jun 01 00:00:00 EDT 2021

Lactate and IL6 define separable paths of inflammatory metabolic adaptation.

Science advances

Dichtl S,Lindenthal L,Zeitler L,Behnke K,Schlösser D,Strobl B,Scheller J,El Kasmi KC,Murray PJ

17-3697-82 was used in Flow Cytometry to test the interrelationships between histone lactylation (Kla) and tissue reparative gene expression.

Tue Jun 01 00:00:00 EDT 2021

Alphavirus-Driven Interferon Gamma (IFNg) Expression Inhibits Tumor Growth in Orthotopic 4T1 Breast Cancer Model.

Vaccines

Trofimova O,Korotkaja K,Skrastina D,Jansons J,Spunde K,Isaguliants M,Zajakina A

Published figure using Arginase 1 monoclonal antibody (Product # 17-3697-82) in Flow Cytometry

Wed Oct 27 00:00:00 EDT 2021

Regulatory T Cells License Macrophage Pro-Resolving Functions During Atherosclerosis Regression.

Circulation research

Sharma M,Schlegel MP,Afonso MS,Brown EJ,Rahman K,Weinstock A,Sansbury BE,Corr EM,van Solingen C,Koelwyn GJ,Shanley LC,Beckett L,Peled D,Lafaille JJ,Spite M,Loke P,Fisher EA,Moore KJ

Published figure using Arginase 1 monoclonal antibody (Product # 17-3697-82) in Immunohistochemistry

Fri Jul 17 00:00:00 EDT 2020

Chinese Medicine Formula PSORI-CM02 Alleviates Psoriatic Dermatitis via M-MDSCs and Th17 Crosstalk.

Frontiers in pharmacology

Deng J,Tan S,Liu R,Yu W,Chen H,Tang N,Han L,Lu C

Published figure using Arginase 1 monoclonal antibody (Product # 17-3697-82) in Flow Cytometry

Sat Feb 06 00:00:00 EST 2021

Metformin as a senostatic drug enhances the anticancer efficacy of CDK4/6 inhibitor in head and neck squamous cell carcinoma.

Cell death & disease

Hu Q,Peng J,Jiang L,Li W,Su Q,Zhang J,Li H,Song M,Cheng B,Xia J,Wu T

17-3697 was used in Flow cytometry/Cell sorting to investigate if metformin can be used as a senostatic drug to modulate senescence-associated secretory phenotype and enhance the anticancer effect of CDK4/6 inhibitors in head and neck squamous cell carcinoma.

Wed Oct 28 00:00:00 EDT 2020

Metformin as a senostatic drug enhances the anticancer efficacy of CDK4/6 inhibitor in head and neck squamous cell carcinoma.

Cell death & disease

Hu Q,Peng J,Jiang L,Li W,Su Q,Zhang J,Li H,Song M,Cheng B,Xia J,Wu T

Wed Oct 28 00:00:00 EDT 2020

Single-Cell RNA Sequencing Reveals a Dynamic Stromal Niche That Supports Tumor Growth.

Cell reports

Davidson S,Efremova M,Riedel A,Mahata B,Pramanik J,Huuhtanen J,Kar G,Vento-Tormo R,Hagai T,Chen X,Haniffa MA,Shields JD,Teichmann SA

17-3697-82 was used in Flow Cytometry to identify complex interplays and increase stromal diversity as a tumor develops, revealing that stromal cells acquire the capacity to modulate immune landscapes from early disease.

Tue May 19 00:00:00 EDT 2020

Regulation of the tumor immune microenvironment and vascular normalization in TNBC murine models by a novel peptide.

Oncoimmunology

Mirando AC,Patil A,Rafie CI,Christmas BJ,Pandey NB,Stearns V,Jaffee EM,Roussos Torres ET,Popel AS

17-3697 was used in Flow cytometry/Cell sorting to demonstrate immune-activating properties of AXT201 that could be developed in combination with other immunomodulatory agents in the treatment of TNBC.

Thu May 14 00:00:00 EDT 2020

Regulation of the tumor immune microenvironment and vascular normalization in TNBC murine models by a novel peptide.

Oncoimmunology

Mirando AC,Patil A,Rafie CI,Christmas BJ,Pandey NB,Stearns V,Jaffee EM,Roussos Torres ET,Popel AS

Thu May 14 00:00:00 EDT 2020

Mesenchymal stromal cells induce distinct myeloid-derived suppressor cells in inflammation.

JCI insight

Lee HJ,Ko JH,Kim HJ,Jeong HJ,Oh JY

17-3697 was used in Flow cytometry/Cell sorting to reveal that suppression of prostaglandin E2 (PGE2) and HGF secretion in MSCs by siRNA transfection partially reversed the effects of MSCs on MDSC differentiation.

Thu Jun 18 00:00:00 EDT 2020

Disruption of Midkine gene reduces traumatic brain injury through the modulation of neuroinflammation.

Journal of neuroinflammation

Takada S,Sakakima H,Matsuyama T,Otsuka S,Nakanishi K,Norimatsu K,Itashiki Y,Tani A,Kikuchi K

17-3697-82 was used in Flow cytometry/Cell sorting to suggest that MK-deficiency reduced tissue infiltration of microglia/macrophages and altered their polarization status thereby reducing neuroinflammation, neuronal apoptosis, and tissue loss and improving neurological outcomes after TBI.

Wed Jan 29 00:00:00 EST 2020

C/EBPβ regulates the M2 transcriptome in β-adrenergic-stimulated macrophages.

Brain, behavior, and immunity

Lamkin DM,Srivastava S,Bradshaw KP,Betz JE,Muy KB,Wiese AM,Yee SK,Waggoner RM,Arevalo JMG,Yoon AJ,Faull KF,Sloan EK,Cole SW

17-3697 was used in Flow cytometry/Cell sorting to identify the role of C/ERBPß in regulating the signalling pathway between ß-adrenergic stimulation and Arg1 expreßion in the M2 transcriptome.

Thu Aug 01 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: A-I; arginase 1 liver; arginase 1, liver; arginase I; arginase, liver; Arginase-1; Arginase1; HGNC:663; Liver Arginase; Liver-type arginase; Type 1 Arginase; Type I arginase

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Gene Aliases: AI; AI256583; Arg-1; ARG1; PGIF

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UniProt ID: (Human) P05089, (Mouse) Q61176

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Entrez Gene ID: (Human) 383, (Mouse) 11846

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Function(s)

arginase activity manganese ion binding hydrolase activity hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines metal ion binding hydrolase

Process(es)

urea cycle liver development positive regulation of endothelial cell proliferation arginine catabolic process aging response to herbicide response to manganese ion response to zinc ion response to selenium ion regulation of L-arginine import response to amine arginine catabolic process to ornithine lung development collagen biosynthetic process response to vitamin A response to vitamin E response to drug response to amino acid response to cadmium ion response to axon injury response to methylmercury mammary gland involution maternal process involved in female pregnancy protein homotrimerization cellular response to hydrogen peroxide cellular response to lipopolysaccharide cellular response to interleukin-4 cellular response to glucagon stimulus cellular response to dexamethasone stimulus cellular response to transforming growth factor beta stimulus arginine metabolic process

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Arginase 1 Monoclonal Antibody (A1exF5), APC, eBioscience™

Arginase 1 Antibody (17-3697-82) in Flow

Product Details

17-3697-82

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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