Invitrogen

Arginase 1 Monoclonal Antibody (A1exF5), PerCP-eFluor™ 710, eBioscience™

Arginase 1 Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 15

Arginase 1 Antibody (46-3697-82) in Flow

C57BL/6 mouse bone marrow derived macrophages were polarized for 24 hours with either LPS (Product # 00-4976-93) and IFN-gamma (Product # 16-7311-85) to make M1 macrophages (left) or with IL-4 (Product # BMS338) to make M2a macrophages (right). Cells were surface stained with F4/80 Monoclonal Antibody, FITC (Product # 11-4801-82) then stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with 0.125 µg Arginase 1 Monoclonal Antibody, PerCP-eFluor 710. Total viable cells ... View More

Arginase 1 Antibody in Immunohistochemistry (IHC)

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Product Details

46-3697-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.25 µg/test

View 17 publications 17 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PerCP-eFluor™ 710, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

A1exF5

Immunogen

E.coli-derived Recombinant mouse Arginase 1

Conjugate

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

Excitation/Emission Max

482/708 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2734843

Product Specific Information

Description: The monoclonal antibody A1exF5 recognizes both human and mouse Arginase 1, a cytosolic enzyme (Arg1). This A1exF5 clone is compatible with both, the standard intracellular protocols, and the Foxp3/Transcription Factor Staining Buffer Set.

Applications Reported: This A1exF5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This A1exF5 antibody has been tested by flow cytometric analysis of stimulated mouse bone marrow cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Target Information

Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, which is the final step in the urea cycle. The resulting polyamines are important for cell proliferation and removal of toxins that arise from protein degradation. By degrading arginine, Arginase 1 deprives NO synthase of its substrate and down-regulates nitric oxide production. In both human and mouse, Arginase 1 is expressed in the liver, neutrophils, myeloid derived suppressor cells (MDSC) and neural stem cells. In human, expression in blood neutrophils but not in CCR3+ granulocytes has been reported. In mice, expression of Arginase 1 is one of the hallmarks of alternatively activated macrophages (M2a). Arginase-1 may be expressed in the myeloid cells infiltrating tumors, and is typically found in the majority of hepatocellular carcinomas. Defects in Arginase 1 are the cause of argininemia, an autosomal recessive disorder characterized by hyperammonemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

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Frontiers in bioengineering and biotechnology

Sheng W,Qin H,Wang T,Zhao J,Fang C,Zhang P,Liu P,Udduttula A,Zeng H,Chen Y

Published figure using Arginase 1 monoclonal antibody (Product # 46-3697-82) in Flow Cytometry

Wed May 31 00:00:00 EDT 2023

Timing of Interleukin-4 Stimulation of Macrophages Determines Their Anti-Microbial Activity during Infection with Salmonella enterica Serovar Typhimurium.

Cells

Brigo N,Neumaier E,Pfeifhofer-Obermair C,Grubwieser P,Engl S,Berger S,Seifert M,Reinstadler V,Oberacher H,Weiss G

Published figure using Arginase 1 monoclonal antibody (Product # 46-3697-82) in Flow Cytometry

Fri Apr 14 00:00:00 EDT 2023

Repurposing nitric oxide donating drugs in cancer therapy through immune modulation.

Journal of experimental & clinical cancer research : CR

Li CY,Anuraga G,Chang CP,Weng TY,Hsu HP,Ta HDK,Su PF,Chiu PH,Yang SJ,Chen FW,Ye PH,Wang CY,Lai MD

Published figure using Arginase 1 monoclonal antibody (Product # 46-3697-82) in Flow Cytometry

Sat Jan 14 00:00:00 EST 2023

Calcaratarin D, a labdane diterpenoid, attenuates mouse asthma via modulating alveolar macrophage function.

British journal of pharmacology

Liao W,Foo HYC,Tran TNQ,Chai CLL,Wong WSF

Published figure using Arginase 1 monoclonal antibody (Product # 46-3697-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2023

Curcumin-dependent phenotypic transformation of microglia mediates resistance to pseudorabies-induced encephalitis.

Veterinary research

Feng L,Luo G,Li Y,Zhang C,Liu Y,Liu Y,Chen H,He D,Zhu Y,Gan L

Published figure using Arginase 1 monoclonal antibody (Product # 46-3697-82) in Flow Cytometry

Tue Mar 14 00:00:00 EDT 2023

Interleukin-10 genetically modified clinical-grade mesenchymal stromal cells markedly reinforced functional recovery after spinal cord injury via directing alternative activation of macrophages.

Cellular & molecular biology letters

Gao T,Huang F,Wang W,Xie Y,Wang B

Published figure using Arginase 1 monoclonal antibody (Product # 46-3697-82) in Flow Cytometry

Thu Mar 17 00:00:00 EDT 2022

Angiotensin 1-7 and its analogue decrease blood pressure but aggravate renal damage in preeclamptic mice.

Experimental animals

Liu Y,Zhai R,Tong J,Yu Y,Yang L,Gu Y,Niu J

Published figure using Arginase 1 monoclonal antibody (Product # 46-3697-82) in Flow Cytometry

Thu Nov 10 00:00:00 EST 2022

LINC00240/miR-155 axis regulates function of trophoblasts and M2 macrophage polarization via modulating oxidative stress-induced pyroptosis in preeclampsia.

Molecular medicine (Cambridge, Mass.)

Wu HY,Liu K,Zhang JL

Published figure using Arginase 1 monoclonal antibody (Product # 46-3697-82) in Flow Cytometry

Sat Sep 24 00:00:00 EDT 2022

Gene-selective transcription promotes the inhibition of tissue reparative macrophages by TNF.

Life science alliance

Dichtl S,Sanin DE,Koss CK,Willenborg S,Petzold A,Tanzer MC,Dahl A,Kabat AM,Lindenthal L,Zeitler L,Satzinger S,Strasser A,Mann M,Roers A,Eming SA,El Kasmi KC,Pearce EJ,Murray PJ

Published figure using Arginase 1 monoclonal antibody (Product # 46-3697-82) in Flow Cytometry

Fri Apr 01 00:00:00 EDT 2022

Toll-like Receptor 9 Pathway Mediates Schlafen⁺-MDSC Polarization During Helicobacter-induced Gastric Metaplasias.

Gastroenterology

Ding L,Chakrabarti J,Sheriff S,Li Q,Thi Hong HN,Sontz RA,Mendoza ZE,Schreibeis A,Helmrath MA,Zavros Y,Merchant JL

46-3697-82 was used in Flow cytometry/Cell sorting to propose that individuals with a more robust response to damage-activated molecular patterns and increased Toll-like receptor 9 (TLR9) expression are predisposed to the neoplastic complications of Helicobacter infection.

Mon Aug 01 00:00:00 EDT 2022

Anisakis pegreffii Extract Induces Airway Inflammation with Airway Remodeling in a Murine Model System.

BioMed research international

Choi JH,Kim JY,Yi MH,Kim M,Yong TS

46-3697-82 was used in Flow Cytometry to confirm that A. pegreffii plays an essential role in causing asthma in mouse models and has the potential to cause similar effects in humans.

Wed Jan 12 00:00:00 EST 2022

Lactate and IL6 define separable paths of inflammatory metabolic adaptation.

Science advances

Dichtl S,Lindenthal L,Zeitler L,Behnke K,Schlösser D,Strobl B,Scheller J,El Kasmi KC,Murray PJ

Published figure using Arginase 1 monoclonal antibody (Product # 46-3697-82) in Flow Cytometry

Tue Jun 01 00:00:00 EDT 2021

Alphavirus-Driven Interferon Gamma (IFNg) Expression Inhibits Tumor Growth in Orthotopic 4T1 Breast Cancer Model.

Vaccines

Trofimova O,Korotkaja K,Skrastina D,Jansons J,Spunde K,Isaguliants M,Zajakina A

Published figure using Arginase 1 monoclonal antibody (Product # 46-3697-82) in Flow Cytometry

Wed Oct 27 00:00:00 EDT 2021

Regulatory T Cells License Macrophage Pro-Resolving Functions During Atherosclerosis Regression.

Circulation research

Sharma M,Schlegel MP,Afonso MS,Brown EJ,Rahman K,Weinstock A,Sansbury BE,Corr EM,van Solingen C,Koelwyn GJ,Shanley LC,Beckett L,Peled D,Lafaille JJ,Spite M,Loke P,Fisher EA,Moore KJ

Published figure using Arginase 1 monoclonal antibody (Product # 46-3697-82) in Immunohistochemistry

Fri Jul 17 00:00:00 EDT 2020

Chinese Medicine Formula PSORI-CM02 Alleviates Psoriatic Dermatitis via M-MDSCs and Th17 Crosstalk.

Frontiers in pharmacology

Deng J,Tan S,Liu R,Yu W,Chen H,Tang N,Han L,Lu C

Published figure using Arginase 1 monoclonal antibody (Product # 46-3697-82) in Flow Cytometry

Sat Feb 06 00:00:00 EST 2021

Metformin as a senostatic drug enhances the anticancer efficacy of CDK4/6 inhibitor in head and neck squamous cell carcinoma.

Cell death & disease

Hu Q,Peng J,Jiang L,Li W,Su Q,Zhang J,Li H,Song M,Cheng B,Xia J,Wu T

Published figure using Arginase 1 monoclonal antibody (Product # 46-3697-82) in Flow Cytometry

Wed Oct 28 00:00:00 EDT 2020

Regulation of the tumor immune microenvironment and vascular normalization in TNBC murine models by a novel peptide.

Oncoimmunology

Mirando AC,Patil A,Rafie CI,Christmas BJ,Pandey NB,Stearns V,Jaffee EM,Roussos Torres ET,Popel AS

Published figure using Arginase 1 monoclonal antibody (Product # 46-3697-82) in Flow Cytometry

Thu May 14 00:00:00 EDT 2020

Adoptive transfer of Trichinella spiralis-activated macrophages can ameliorate both Th1- and Th2-activated inflammation in murine models.

Scientific reports

Kang SA,Park MK,Park SK,Choi JH,Lee DI,Song SM,Yu HS

46-3697-82 was used in Flow cytometry/Cell sorting to compare the effects of M1 and M2 macrophages obtained from Trichinella spiralis-infected mice with those of T. spiralis excretory/secretory (ES) protein-treated macrophages on experimental intestinal inflammation and allergic airway inflammation.

Thu Apr 25 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: A-I; arginase 1 liver; arginase 1, liver; arginase I; arginase, liver; Arginase-1; Arginase1; HGNC:663; Liver Arginase; Liver-type arginase; Type 1 Arginase; Type I arginase

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Gene Aliases: AI; AI256583; Arg-1; ARG1; PGIF

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UniProt ID: (Human) P05089, (Mouse) Q61176

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Entrez Gene ID: (Human) 383, (Mouse) 11846

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Function(s)

arginase activity manganese ion binding hydrolase activity hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines metal ion binding hydrolase

Process(es)

urea cycle liver development positive regulation of endothelial cell proliferation arginine catabolic process aging response to herbicide response to manganese ion response to zinc ion response to selenium ion regulation of L-arginine import response to amine arginine catabolic process to ornithine lung development collagen biosynthetic process response to vitamin A response to vitamin E response to drug response to amino acid response to cadmium ion response to axon injury response to methylmercury mammary gland involution maternal process involved in female pregnancy protein homotrimerization cellular response to hydrogen peroxide cellular response to lipopolysaccharide cellular response to interleukin-4 cellular response to glucagon stimulus cellular response to dexamethasone stimulus cellular response to transforming growth factor beta stimulus arginine metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Arginase 1 Monoclonal Antibody (A1exF5), PerCP-eFluor™ 710, eBioscience™

Arginase 1 Antibody (46-3697-82) in Flow

Product Details

46-3697-82

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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