Invitrogen

Arginase 1 Monoclonal Antibody (A1exF5), Alexa Fluor™ 488, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

16 Published Figures

23 References

View all (94) Arginase 1 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Arginase 1 Antibody in Flow Cytometry (Flow)

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Arginase 1 Antibody (53-3697-82) in Flow

Normal human lysed whole blood cells were stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with 0.5 µg of Arginase 1 Monoclonal Antibody, Alexa Fluor 488. Cells were co-stained with either CD16 Monoclonal Antibody, APC (Product # 17-0168-42) or CD193 Monoclonal Antibody, PE (Product # 12-1939-42). Cells in the granulocyte gate (left panel) were used for analysis and were gated on either the CD193+ population (blue histogram) or the CD16+ population (purple histogra... View More

Arginase 1 Antibody in Immunocytochemistry (ICC/IF)

Arginase 1 Antibody in Immunohistochemistry (IHC)

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Product Details

53-3697-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

1.0 µg/test

View 18 publications 18 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

A1exF5

Immunogen

E.coli-derived recombinant mouse Arginase 1

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2734831

Product Specific Information

Description: The monoclonal antibody A1exF5 recognizes both human and mouse Arginase 1, a cytosolic enzyme (Arg1). This A1exF5 clone is compatible with both, the standard intracellular protocols, and the Foxp3/Transcription Factor Staining Buffer Set.

Applications Reported: This A1exF5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This A1exF5 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Target Information

Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, which is the final step in the urea cycle. The resulting polyamines are important for cell proliferation and removal of toxins that arise from protein degradation. By degrading arginine, Arginase 1 deprives NO synthase of its substrate and down-regulates nitric oxide production. In both human and mouse, Arginase 1 is expressed in the liver, neutrophils, myeloid derived suppressor cells (MDSC) and neural stem cells. In human, expression in blood neutrophils but not in CCR3+ granulocytes has been reported. In mice, expression of Arginase 1 is one of the hallmarks of alternatively activated macrophages (M2a). Arginase-1 may be expressed in the myeloid cells infiltrating tumors, and is typically found in the majority of hepatocellular carcinomas. Defects in Arginase 1 are the cause of argininemia, an autosomal recessive disorder characterized by hyperammonemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Advanced phosphocreatine-grafted chitosan hydrogel promote wound healing by macrophage modulation.

Frontiers in bioengineering and biotechnology

Sheng W,Qin H,Wang T,Zhao J,Fang C,Zhang P,Liu P,Udduttula A,Zeng H,Chen Y

Published figure using Arginase 1 monoclonal antibody (Product # 53-3697-82) in Flow Cytometry

Wed May 31 00:00:00 EDT 2023

Timing of Interleukin-4 Stimulation of Macrophages Determines Their Anti-Microbial Activity during Infection with Salmonella enterica Serovar Typhimurium.

Cells

Brigo N,Neumaier E,Pfeifhofer-Obermair C,Grubwieser P,Engl S,Berger S,Seifert M,Reinstadler V,Oberacher H,Weiss G

Published figure using Arginase 1 monoclonal antibody (Product # 53-3697-82) in Flow Cytometry

Fri Apr 14 00:00:00 EDT 2023

Repurposing nitric oxide donating drugs in cancer therapy through immune modulation.

Journal of experimental & clinical cancer research : CR

Li CY,Anuraga G,Chang CP,Weng TY,Hsu HP,Ta HDK,Su PF,Chiu PH,Yang SJ,Chen FW,Ye PH,Wang CY,Lai MD

Published figure using Arginase 1 monoclonal antibody (Product # 53-3697-82) in Flow Cytometry

Sat Jan 14 00:00:00 EST 2023

Calcaratarin D, a labdane diterpenoid, attenuates mouse asthma via modulating alveolar macrophage function.

British journal of pharmacology

Liao W,Foo HYC,Tran TNQ,Chai CLL,Wong WSF

53-3697-82 was used in Flow cytometry/Cell sorting to demonstrate for the first time that CalD is a novel anti-inflammatory natural compound for allergic asthma that modulates AM function.

Sat Apr 01 00:00:00 EDT 2023

Calcaratarin D, a labdane diterpenoid, attenuates mouse asthma via modulating alveolar macrophage function.

British journal of pharmacology

Liao W,Foo HYC,Tran TNQ,Chai CLL,Wong WSF

53-3697-82 was used in Flow cytometry/Cell sorting to demonstrate for the first time that CalD is a novel anti-inflammatory natural compound for allergic asthma that modulates AM function.

Sat Apr 01 00:00:00 EDT 2023

Glucocorticoid activation by HSD11B1 limits T cell-driven interferon signaling and response to PD-1 blockade in melanoma.

Journal for immunotherapy of cancer

Martins Nascentes Melo L,Herrera-Rios D,Hinze D,Löffek S,Oezel I,Turiello R,Klein J,Leonardelli S,Westedt IV,Al-Matary Y,Egea-Rodriguez S,Brenzel A,Bau M,Sucker A,Hadaschik E,Wirsdörfer F,Hanenberg H,Uhlenbrock N,Rauh D,Poźniak J,Rambow F,Marine JC,Effern M,Glodde N,Schadendorf D,Jablonska J,Hölzel M,Helfrich I

53-3697-82 was used in Flow cytometry/Cell sorting to suggest a drug repurposing strategy combining HSD11B1 inhibitors with ICIs to improve melanoma immunotherapy.

Fri Apr 07 00:00:00 EDT 2023

Curcumin-dependent phenotypic transformation of microglia mediates resistance to pseudorabies-induced encephalitis.

Veterinary research

Feng L,Luo G,Li Y,Zhang C,Liu Y,Liu Y,Chen H,He D,Zhu Y,Gan L

Published figure using Arginase 1 monoclonal antibody (Product # 53-3697-82) in Flow Cytometry

Tue Mar 14 00:00:00 EDT 2023

Interleukin-10 genetically modified clinical-grade mesenchymal stromal cells markedly reinforced functional recovery after spinal cord injury via directing alternative activation of macrophages.

Cellular & molecular biology letters

Gao T,Huang F,Wang W,Xie Y,Wang B

Published figure using Arginase 1 monoclonal antibody (Product # 53-3697-82) in Flow Cytometry

Thu Mar 17 00:00:00 EDT 2022

Angiotensin 1-7 and its analogue decrease blood pressure but aggravate renal damage in preeclamptic mice.

Experimental animals

Liu Y,Zhai R,Tong J,Yu Y,Yang L,Gu Y,Niu J

Published figure using Arginase 1 monoclonal antibody (Product # 53-3697-82) in Flow Cytometry

Thu Nov 10 00:00:00 EST 2022

LINC00240/miR-155 axis regulates function of trophoblasts and M2 macrophage polarization via modulating oxidative stress-induced pyroptosis in preeclampsia.

Molecular medicine (Cambridge, Mass.)

Wu HY,Liu K,Zhang JL

Published figure using Arginase 1 monoclonal antibody (Product # 53-3697-82) in Flow Cytometry

Sat Sep 24 00:00:00 EDT 2022

Gene-selective transcription promotes the inhibition of tissue reparative macrophages by TNF.

Life science alliance

Dichtl S,Sanin DE,Koss CK,Willenborg S,Petzold A,Tanzer MC,Dahl A,Kabat AM,Lindenthal L,Zeitler L,Satzinger S,Strasser A,Mann M,Roers A,Eming SA,El Kasmi KC,Pearce EJ,Murray PJ

Published figure using Arginase 1 monoclonal antibody (Product # 53-3697-82) in Flow Cytometry

Fri Apr 01 00:00:00 EDT 2022

Cancer cell autophagy, reprogrammed macrophages, and remodeled vasculature in glioblastoma triggers tumor immunity.

Cancer cell

Chryplewicz A,Scotton J,Tichet M,Zomer A,Shchors K,Joyce JA,Homicsko K,Hanahan D

53-3697-82 was used in flow cytometry to assess this concept by combining vascular endothelial growth factor (VEGF) pathway inhibitors that remodel the tumor vasculature with the tricyclic antidepressant imipramine, which enhances autophagy in GBM cancer cells and unexpectedly reprograms immunosuppressive tumor-associated macrophages via inhibition of histamine receptor signaling to become immunostimulatory.

Mon Oct 10 00:00:00 EDT 2022

In vivo single-cell transcriptomics reveal Klebsiella pneumoniae skews lung macrophages to promote infection.

EMBO molecular medicine

Dumigan A,Cappa O,Morris B,Sá Pessoa J,Calderon-Gonzalez R,Mills G,Lancaster R,Simpson D,Kissenpfennig A,Bengoechea JA

53-3697-82 was used in Flow cytometry/Cell sorting to identify interstitial macrophages (IMs) as the main population of lung macrophages associated with Klebsiella.

Wed Dec 07 00:00:00 EST 2022

Lactate and IL6 define separable paths of inflammatory metabolic adaptation.

Science advances

Dichtl S,Lindenthal L,Zeitler L,Behnke K,Schlösser D,Strobl B,Scheller J,El Kasmi KC,Murray PJ

Published figure using Arginase 1 monoclonal antibody (Product # 53-3697-82) in Flow Cytometry

Tue Jun 01 00:00:00 EDT 2021

Alphavirus-Driven Interferon Gamma (IFNg) Expression Inhibits Tumor Growth in Orthotopic 4T1 Breast Cancer Model.

Vaccines

Trofimova O,Korotkaja K,Skrastina D,Jansons J,Spunde K,Isaguliants M,Zajakina A

Published figure using Arginase 1 monoclonal antibody (Product # 53-3697-82) in Flow Cytometry

Wed Oct 27 00:00:00 EDT 2021

Atherosclerosis Regression and Cholesterol Efflux in Hypertriglyceridemic Mice.

Circulation research

Josefs T,Basu D,Vaisar T,Arets B,Kanter JE,Huggins LA,Hu Y,Liu J,Clouet-Foraison N,Heinecke JW,Bornfeldt KE,Goldberg IJ,Fisher EA

53-3697-82 was used in Immunohistochemistry (Frozen) to assess how HyperTG and reduced HDL-C affect regression of atherosclerosis and the phenotype of plaque macrophages in atherosclerotic mice.

Fri Mar 19 00:00:00 EDT 2021

Macrophage Metabolism of Apoptotic Cell-Derived Arginine Promotes Continual Efferocytosis and Resolution of Injury.

Cell metabolism

Yurdagul A,Subramanian M,Wang X,Crown SB,Ilkayeva OR,Darville L,Kolluru GK,Rymond CC,Gerlach BD,Zheng Z,Kuriakose G,Kevil CG,Koomen JM,Cleveland JL,Muoio DM,Tabas I

53-3697-82 was used in Immunohistochemistry (Paraffin) to conclude that macrophage metabolism of AC-derived metabolites allows for optimal continual efferocytosis and resolution of injury.

Tue Mar 03 00:00:00 EST 2020

Macrophage Metabolism of Apoptotic Cell-Derived Arginine Promotes Continual Efferocytosis and Resolution of Injury.

Cell metabolism

Yurdagul A,Subramanian M,Wang X,Crown SB,Ilkayeva OR,Darville L,Kolluru GK,Rymond CC,Gerlach BD,Zheng Z,Kuriakose G,Kevil CG,Koomen JM,Cleveland JL,Muoio DM,Tabas I

53-3697-82 was used in Immunohistochemistry (Paraffin) to conclude that macrophage metabolism of AC-derived metabolites allows for optimal continual efferocytosis and resolution of injury.

Tue Mar 03 00:00:00 EST 2020

Regulatory T Cells License Macrophage Pro-Resolving Functions During Atherosclerosis Regression.

Circulation research

Sharma M,Schlegel MP,Afonso MS,Brown EJ,Rahman K,Weinstock A,Sansbury BE,Corr EM,van Solingen C,Koelwyn GJ,Shanley LC,Beckett L,Peled D,Lafaille JJ,Spite M,Loke P,Fisher EA,Moore KJ

53-3697-82 was used in Immunohistochemistry (PFA fixed) to establish essential roles for Tregs in resolving atherosclerotic cardiovascular disease and provide mechanistic insight into the pathways governing plaque remodeling and regression of disease.

Fri Jul 17 00:00:00 EDT 2020

Regulatory T Cells License Macrophage Pro-Resolving Functions During Atherosclerosis Regression.

Circulation research

Sharma M,Schlegel MP,Afonso MS,Brown EJ,Rahman K,Weinstock A,Sansbury BE,Corr EM,van Solingen C,Koelwyn GJ,Shanley LC,Beckett L,Peled D,Lafaille JJ,Spite M,Loke P,Fisher EA,Moore KJ

Fri Jul 17 00:00:00 EDT 2020

Chinese Medicine Formula PSORI-CM02 Alleviates Psoriatic Dermatitis via M-MDSCs and Th17 Crosstalk.

Frontiers in pharmacology

Deng J,Tan S,Liu R,Yu W,Chen H,Tang N,Han L,Lu C

Published figure using Arginase 1 monoclonal antibody (Product # 53-3697-82) in Flow Cytometry

Sat Feb 06 00:00:00 EST 2021

Metformin as a senostatic drug enhances the anticancer efficacy of CDK4/6 inhibitor in head and neck squamous cell carcinoma.

Cell death & disease

Hu Q,Peng J,Jiang L,Li W,Su Q,Zhang J,Li H,Song M,Cheng B,Xia J,Wu T

Published figure using Arginase 1 monoclonal antibody (Product # 53-3697-82) in Flow Cytometry

Wed Oct 28 00:00:00 EDT 2020

Single-Cell RNA Sequencing Reveals a Dynamic Stromal Niche That Supports Tumor Growth.

Cell reports

Davidson S,Efremova M,Riedel A,Mahata B,Pramanik J,Huuhtanen J,Kar G,Vento-Tormo R,Hagai T,Chen X,Haniffa MA,Shields JD,Teichmann SA

Published figure using Arginase 1 monoclonal antibody (Product # 53-3697-82) in Flow Cytometry

Tue May 19 00:00:00 EDT 2020

Regulation of the tumor immune microenvironment and vascular normalization in TNBC murine models by a novel peptide.

Oncoimmunology

Mirando AC,Patil A,Rafie CI,Christmas BJ,Pandey NB,Stearns V,Jaffee EM,Roussos Torres ET,Popel AS

Published figure using Arginase 1 monoclonal antibody (Product # 53-3697-82) in Flow Cytometry

Thu May 14 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: A-I; arginase 1 liver; arginase 1, liver; arginase I; arginase, liver; Arginase-1; Arginase1; HGNC:663; Liver Arginase; Liver-type arginase; Type 1 Arginase; Type I arginase

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Gene Aliases: AI; AI256583; Arg-1; ARG1; PGIF

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UniProt ID: (Human) P05089, (Mouse) Q61176

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Entrez Gene ID: (Human) 383, (Mouse) 11846

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Function(s)

arginase activity manganese ion binding hydrolase activity hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines metal ion binding hydrolase

Process(es)

urea cycle liver development positive regulation of endothelial cell proliferation arginine catabolic process aging response to herbicide response to manganese ion response to zinc ion response to selenium ion regulation of L-arginine import response to amine arginine catabolic process to ornithine lung development collagen biosynthetic process response to vitamin A response to vitamin E response to drug response to amino acid response to cadmium ion response to axon injury response to methylmercury mammary gland involution maternal process involved in female pregnancy protein homotrimerization cellular response to hydrogen peroxide cellular response to lipopolysaccharide cellular response to interleukin-4 cellular response to glucagon stimulus cellular response to dexamethasone stimulus cellular response to transforming growth factor beta stimulus arginine metabolic process

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Arginase 1 Monoclonal Antibody (A1exF5), Alexa Fluor™ 488, eBioscience™

Arginase 1 Antibody (53-3697-82) in Flow

Product Details

53-3697-82

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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