Invitrogen

FOXP3 Monoclonal Antibody (PCH101), PE-Cyanine7, eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

16 References

View all (163) FOXP3 antibodies

Datasheet

Protocols

Questions & Answers

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Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (1)
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FOXP3 Antibody (25-4776-42) in Flow

Intracellular staining of normal human peripheral blood cells with Anti-Human/Non-Human Primate CD25 PerCP-eFluor® 710 (Product # 46-0257-42) and Rat IgG2a K Isotype Control PE-Cyanine7 (Product # 25-4321-82) (left) or Anti-Human Foxp3 PE-Cyanine7 (right) using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

FOXP3 Antibody — Intracellular staining of human peripheral blood cells. As expected based on known relative expression patterns, Foxp3 clone PCH101 stains a subset of the CD4+ T cells and does not stain the CD8+ T cells. Details: Normal human peripheral blood cells were surface stained with CD3 (clone UCHT1), CD4 (clone RPA-T4, top), and CD8 (clone OKT8, bottom), followed by intracellular staining with Rat IgG2a kappa Isotype Control (left) or Foxp3 (clone PCH101, right) using the Foxp3/Transcription Factor Staining Buffer Set and protocol. Lymphocytes in the CD3+ gate were used for analysis. {RE}

Product Details

25-4776-42

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 15 publications 15 publications

Product Specifications

Species Reactivity

Chimpanzee, Cynomolgus monkey, Human, Non-human primate, Rhesus monkey

Published species

Human

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

PCH101

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10804638

Product Specific Information

Description: eBioscience offers a panel of monoclonal antibodies to different epitopes of human Foxp3, providing useful tools for investigating the complete expression pattern of Foxp3 at the protein level, and discerning the precise subsets of Foxp3^+ cells.

The PCH101 antibody reacts with the amino terminus of human foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.

Intracellular staining of human peripheral blood mononuclear cells (PBMCs) with PCH101 antibody using the anti-human Foxp3 Staining Set and protocol reveals approximately 0.5-4% of lymphocytes staining, with the majority of staining occurring in the CD25^bright population. This is subject to donor variability.

PCH101 crossreacts with rhesus, chimpanzee and cynomolgus. We recommend the use of CD4 (OKT4, Product # 11-0048-42 , or RPA-T4, Product # 11-0049-42 , depending on the species) and CD25 (BC96, Product # 17-0259-42).

Applications Reported: This PCH101 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This PCH101 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Buffer Set (Product # 00-5523). Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Immune profile analysis of peripheral blood and tumors of lung cancer patients treated with immune checkpoint inhibitors.

Translational lung cancer research

Ichiki Y,Fukuyama T,Ueno M,Kanasaki Y,Goto H,Takahashi M,Mikami S,Kobayashi N,Nakanishi K,Hayashi S,Ishida T

25-4776-42 was used in Flow cytometry/Cell sorting to analyze the association between the immune-related molecular expression in peripheral blood mononuclear cells (PBMCs) and lung cancer tissues, and the effects of ICI monotherapy.

Tue Nov 01 00:00:00 EDT 2022

Combined TCR Repertoire Profiles and Blood Cell Phenotypes Predict Melanoma Patient Response to Personalized Neoantigen Therapy plus Anti-PD-1.

Cell reports. Medicine

Poran A,Scherer J,Bushway ME,Besada R,Balogh KN,Wanamaker A,Williams RG,Prabhakara J,Ott PA,Hu-Lieskovan S,Khondker ZS,Gaynor RB,Rooney MS,Srinivasan L

25-4776 was used in Flow cytometry/Cell sorting to characterize the TCR repertoires and T and B cell frequencies in 21 patients with metastatic melanoma who received this regimen.

Tue Nov 17 00:00:00 EST 2020

Comparative Analysis of the Regulatory T Cells Dynamics in Peripheral Blood in Human and Porcine Polytrauma.

Frontiers in immunology

Serve R,Sturm R,Schimunek L,Störmann P,Heftrig D,Teuben MPJ,Oppermann E,Horst K,Pfeifer R,Simon TP,Kalbas Y,Pape HC,Hildebrand F,Marzi I,Relja B

25-4776 was used in Flow cytometry/Cell sorting to demonstrate a comparable decrease of Treg early after polytrauma, and strengthen the immunological significance of the porcine polytrauma model and that Treg subpopulation CD4+CD25+CD127- was characterized in porcine samples.

Mon Jun 24 00:00:00 EDT 2019

Injection of Adipose-Derived Stromal Cells in the Knee of Patients with Severe Osteoarthritis has a Systemic Effect and Promotes an Anti-Inflammatory Phenotype of Circulating Immune Cells.

Theranostics

Pers YM,Quentin J,Feirreira R,Espinoza F,Abdellaoui N,Erkilic N,Cren M,Dufourcq-Lopez E,Pullig O,Nöth U,Jorgensen C,Louis-Plence P

25-4776 was used in Flow cytometry/Cell sorting to study the effect of autologous adipose-derived stromal cells intra-articular injection.

Mon Sep 09 00:00:00 EDT 2019

Intratumoral FoxP3⁺Helios⁺ Regulatory T Cells Upregulating Immunosuppressive Molecules Are Expanded in Human Colorectal Cancer.

Frontiers in immunology

Syed Khaja AS,Toor SM,El Salhat H,Ali BR,Elkord E

25-4776 was used in Flow cytometry/Cell sorting to characterise the differences in immune cells in the tumour microenvironment of colorectal cancer patients compared to paired adjacent non-tumour colon tissue of CRC patients.

Sun Jan 03 00:00:00 EST 2021

Phenotypic characterization of regulatory T cells from antiretroviral-naive HIV-1-infected people.

Immunology

Ambada GN,Ntsama CE,Nji NN,Ngu LN,Sake CN,Lissom A,Tchouangeu FT,Tchadji J,Sosso M,Etoa FX,Nchinda GW

25-4776 was used in Flow cytometry/Cell sorting to determine how antiretroviral therapy-naive HIV-1 infection affects the phenotypic properties of Treg cells.

Tue Aug 01 00:00:00 EDT 2017

Combining FoxP3 and Helios with GARP/LAP markers can identify expanded Treg subsets in cancer patients.

Oncotarget

Abd Al Samid M,Chaudhary B,Khaled YS,Ammori BJ,Elkord E

25-4776 was used in Flow cytometry/Cell sorting to demonstrate that studying a combination of Treg-specific markers could be used to more accurately determine expanded Treg subsets and to understand their contribution in cancer settings.

Tue Mar 22 00:00:00 EDT 2016

PD-1+Tim-3+ CD8+ T Lymphocytes Display Varied Degrees of Functional Exhaustion in Patients with Regionally Metastatic Differentiated Thyroid Cancer.

Cancer immunology research

Severson JJ,Serracino HS,Mateescu V,Raeburn CD,McIntyre RC,Sams SB,Haugen BR,French JD

25-4776 was used in Flow cytometry/Cell sorting to determine that the release of PD-1 and Tim-3, and the inhibition of Tregs, may encourage tumour elimination in metastatic differentiated thyroid cancer.

Mon Jun 01 00:00:00 EDT 2015

The immunosuppressive enzyme IL4I1 promotes FoxP3(+) regulatory T lymphocyte differentiation.

European journal of immunology

Cousin C,Aubatin A,Le Gouvello S,Apetoh L,Castellano F,Molinier-Frenkel V

Mon Jun 01 00:00:00 EDT 2015

Synovial Regulatory T Cells Occupy a Discrete TCR Niche in Human Arthritis and Require Local Signals To Stabilize FOXP3 Protein Expression.

Journal of immunology (Baltimore, Md. : 1950)

Bending D,Giannakopoulou E,Lom H,Wedderburn LR

25-4776 was used in Flow cytometry/Cell sorting to identify a unique population of Treg cells which arise from separate lineages to conventional T cells in response to chronic inflammatory conditions.

Tue Dec 15 00:00:00 EST 2015

Helios, and not FoxP3, is the marker of activated Tregs expressing GARP/LAP.

Oncotarget

Elkord E,Abd Al Samid M,Chaudhary B

25-4776 was used in Flow cytometry/Cell sorting to define reliable and specific markers that can distinguish different Treg subpopulations.

Fri Aug 21 00:00:00 EDT 2015

A CD8 T cell/indoleamine 2,3-dioxygenase axis is required for mesenchymal stem cell suppression of human systemic lupus erythematosus.

Arthritis & rheumatology (Hoboken, N.J.)

Wang D,Feng X,Lu L,Konkel JE,Zhang H,Chen Z,Li X,Gao X,Lu L,Shi S,Chen W,Sun L

25-4776 was used in Flow cytometry/Cell sorting to highlight a previously unrecognised CD8+ T cell/IFNγ/IDO axis that mediates the therapeutic effects of allogeneic MSCs in lupus patients.

Fri Aug 01 00:00:00 EDT 2014

Elevated levels of CD4(+)CD25(+)FoxP3(+) T cells in systemic sclerosis patients contribute to the secretion of IL-17 and immunosuppression dysfunction.

PloS one

Liu X,Gao N,Li M,Xu D,Hou Y,Wang Q,Zhang G,Sun Q,Zhang H,Zeng X

25-4776 was used in Flow cytometry/Cell sorting to investigate the role of Treg subsets in the immune imbalance in naive systemic sclerosis (SSc), showing that elevated levels of CD4(+)CD25(+)FoxP3(+) T cells contribute to the secretion of IL-17 and immunosuppression dysfunction.

Thu Jan 16 00:00:00 EST 2014

Dynamic development of homing receptor expression and memory cell differentiation of infant CD4+CD25high regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Grindebacke H,Stenstad H,Quiding-Järbrink M,Waldenström J,Adlerberth I,Wold AE,Rudin A

25-4776 was used in Flow cytometry/Cell sorting to suggest that the gut is the primary site of Treg stimulation to exogenous Ags during the first 18 months of life.

Thu Oct 01 00:00:00 EDT 2009

Mucosal but not peripheral FOXP3+ regulatory T cells are highly increased in untreated HIV infection and normalize after suppressive HAART.

Blood

Epple HJ,Loddenkemper C,Kunkel D,Tröger H,Maul J,Moos V,Berg E,Ullrich R,Schulzke JD,Stein H,Duchmann R,Zeitz M,Schneider T

Wed Nov 01 00:00:00 EST 2006

Cutting edge: direct suppression of B cells by CD4+ CD25+ regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Lim HW,Hillsamer P,Banham AH,Kim CH

Sat Oct 01 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: Forkhead box protein P3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; MGC141961; MGC141963; regulatory protein Foxp3; RP23-54C14.1; Scurfin

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Gene Aliases: AIID; DIETER; FOXP3; IPEX; JM2; PIDX; XPID

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UniProt ID: (Human) Q9BZS1, (Cynomolgus monkey) Q6U8D7

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Entrez Gene ID: (Human) 50943, (Cynomolgus monkey) 102120882, (Rhesus monkey) 574303

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Function(s)

RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription corepressor activity protein binding histone acetyltransferase binding protein homodimerization activity histone deacetylase binding sequence-specific DNA binding metal ion binding NF-kappaB binding NFAT protein binding

Process(es)

Performance Guarantee

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FOXP3 Monoclonal Antibody (PCH101), PE-Cyanine7, eBioscience™

FOXP3 Antibody (25-4776-42) in Flow

Product Details

25-4776-42

Immunohistochemistry (Paraffin) (IHC (P))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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