Invitrogen

CD40 Monoclonal Antibody (HM40-3), eFluor™ 450, eBioscience™

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CD40 Antibody (48-0402-82) in Flow

Staining of mouse splenocytes with Anti-Human/Mouse CD45R (B220) PerCP-eFluor® 710 (Product # 46-0452-82) and 0.25 µg of Anti-Mouse/Rat CD40 eFluor® 450. Cells in the lymphocyte gate were used for analysis.

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Product Details

48-0402-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 13 publications 13 publications

In vitro Assay (IV)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Mouse

Host/Isotype

Armenian hamster / IgM, kappa

Class

Monoclonal

Type

Antibody

Clone

HM40-3

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2574017

Product Specific Information

Description: The HM40-3 monoclonal antibody reacts with mouse and rat CD40, a 45-50 kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by mouse B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development/maturation by inducing Ig isotype switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39), its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation.

Applications Reported: This HM40-3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HM40-3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD40 antigen is a single chain glycoprotein that is known to be a member of the tumor necrosis factor/nerve growth factor superfamily and shows a significant homology to the Hodgkin's disease associated antigen, CD30. CD40 is present on all B cells except plasma cells and is also found on some epithelial cells, carcinomas and lymphoid dendritic cells. CD40 has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of CD40, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with CD40 and serves as a mediator of the signal transduction. The interaction of CD40 and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Two alternatively spliced transcript variants of CD40 encoding distinct isoforms have been reported. Diseases associated with CD40 dysfunction include Type 3 Hyper-Igm immunodeficiency and CD40 ligand deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

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Scientific reports

Yan C,Kuang W,Jin L,Wang R,Niu L,Xie C,Ding J,Liao Y,Wang L,Wan H,Ma G

Published figure using CD40 monoclonal antibody (Product # 48-0402-82) in Flow Cytometry

Mon Aug 07 00:00:00 EDT 2023

Curcumin-dependent phenotypic transformation of microglia mediates resistance to pseudorabies-induced encephalitis.

Veterinary research

Feng L,Luo G,Li Y,Zhang C,Liu Y,Liu Y,Chen H,He D,Zhu Y,Gan L

Published figure using CD40 monoclonal antibody (Product # 48-0402-82) in Flow Cytometry

Tue Mar 14 00:00:00 EDT 2023

PP2Cδ Controls the Differentiation and Function of Dendritic Cells Through Regulating the NSD2/mTORC2/ACLY Pathway.

Frontiers in immunology

Lv N,Jin S,Liang Z,Wu X,Kang Y,Su L,Dong Y,Wang B,Ma T,Shi L

Published figure using CD40 monoclonal antibody (Product # 48-0402-82) in Flow Cytometry

Mon Feb 28 00:00:00 EST 2022

A genotype-phenotype screening system using conditionally immortalized immature dendritic cells.

STAR protocols

Zhao L,Liu P,Xie W,Zhang S,Thieme S,Zitvogel L,Kroemer G,Kepp O

48-0402-82 was used in Flow Cytometry to allow investigators to link the genotype of DCs to their phenotype.

Fri Sep 17 00:00:00 EDT 2021

Dual-Functionalized MSCs that Express CX3CR1 and IL-25 Exhibit Enhanced Therapeutic Effects on Inflammatory Bowel Disease.

Molecular therapy : the journal of the American Society of Gene Therapy

Fu Y,Ni J,Chen J,Ma G,Zhao M,Zhu S,Shi T,Zhu J,Huang Z,Zhang J,Chen J

48-0402 was used in Flow cytometry/Cell sorting to demonstrate a new methodology to manipulate MSCs to express CX3C chemokine receptor 1 (CX3CR1) and interleukin-25 (IL-25) to promote their delivery to the inflamed colon and enhance their immunosuppressive capability.

Wed Apr 08 00:00:00 EDT 2020

REV7 is required for processing AID initiated DNA lesions in activated B cells.

Nature communications

Yang D,Sun Y,Chen J,Zhang Y,Fan S,Huang M,Xie X,Cai Y,Shang Y,Gui T,Sun L,Hu J,Dong J,Yeap LS,Wang X,Xiao W,Meng FL

Published figure using CD40 monoclonal antibody (Product # 48-0402-82) in In vitro Assay

Thu Jun 04 00:00:00 EDT 2020

REV7 is required for processing AID initiated DNA lesions in activated B cells.

Nature communications

Yang D,Sun Y,Chen J,Zhang Y,Fan S,Huang M,Xie X,Cai Y,Shang Y,Gui T,Sun L,Hu J,Dong J,Yeap LS,Wang X,Xiao W,Meng FL

Published figure using CD40 monoclonal antibody (Product # 48-0402-82) in In vitro Assay

Thu Jun 04 00:00:00 EDT 2020

TET enzymes control antibody production and shape the mutational landscape in germinal centre B cells.

The FEBS journal

Schoeler K,Aufschnaiter A,Messner S,Derudder E,Herzog S,Villunger A,Rajewsky K,Labi V

Published figure using CD40 monoclonal antibody (Product # 48-0402-82) in Flow Cytometry

Sun Sep 01 00:00:00 EDT 2019

cGAS/STING/TBK1/IRF3 Signaling Pathway Activates BMDCs Maturation Following Mycobacterium bovis Infection.

International journal of molecular sciences

Li Q,Liu C,Yue R,El-Ashram S,Wang J,He X,Zhao D,Zhou X,Xu L

Published figure using CD40 monoclonal antibody (Product # 48-0402-82) in Flow Cytometry

Tue Feb 19 00:00:00 EST 2019

Effect of immune tolerance induced by immature dendritic cells and CTLA4-Ig on systemic lupus erythematosus: An in vivo study.

Experimental and therapeutic medicine

Huang C,Zhang L,Ling F,Wen S,Luo Y,Liu H,Liu J,Zheng W,Liang M,Sun J,Lin YK

Published figure using CD40 monoclonal antibody (Product # 48-0402-82) in Flow Cytometry

Thu Mar 01 00:00:00 EST 2018

Environmental sensing by mature B cells is controlled by the transcription factors PU.1 and SpiB.

Nature communications

Willis SN,Tellier J,Liao Y,Trezise S,Light A,O'Donnell K,Garrett-Sinha LA,Shi W,Tarlinton DM,Nutt SL

Published figure using CD40 monoclonal antibody (Product # 48-0402-82) in Flow Cytometry

Fri Nov 10 00:00:00 EST 2017

Der f 31, a novel allergen from Dermatophagoides farinae, activates epithelial cells and enhances lung-resident group 2 innate lymphoid cells.

Scientific reports

Wang H,Lin J,Zeng L,Ouyang C,Ran P,Yang P,Liu Z

Published figure using CD40 monoclonal antibody (Product # 48-0402-82) in Flow Cytometry

Thu Aug 17 00:00:00 EDT 2017

Thiol peroxiredoxin, a novel allergen from Bombyx mori, modulates functions of macrophages and dendritic cells.

American journal of translational research

Wang H,Hu W,Liang Z,Zeng L,Li J,Yan H,Yang P,Liu Z,Wang L

Published figure using CD40 monoclonal antibody (Product # 48-0402-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

DNA damage activates a complex transcriptional response in murine lymphocytes that includes both physiological and cancer-predisposition programs.

BMC genomics

Innes CL,Hesse JE,Palii SS,Helmink BA,Holub AJ,Sleckman BP,Paules RS

Published figure using CD40 monoclonal antibody (Product # 48-0402-82) in Flow Cytometry

Tue Mar 12 00:00:00 EDT 2013

Immunogenicity and protective capacity of a virosomal respiratory syncytial virus vaccine adjuvanted with monophosphoryl lipid A in mice.

PloS one

Kamphuis T,Meijerhof T,Stegmann T,Lederhofer J,Wilschut J,de Haan A

Published figure using CD40 monoclonal antibody (Product # 48-0402-82) in Flow Cytometry

Wed Sep 19 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: B-cell surface antigen CD40; Bp50; CD antigen CD40; CD40; CD40 antigen, TNF receptor superfamily member 5; CD40L receptor; Immunoglobulin M; ImmunoglobulinM; MGC9013; sCD40; soluble CD40; T-cell differentiation antigen; Tumor necrosis factor receptor superfamily member 5; tumor necrosis factor receptor superfamily, member 5

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Gene Aliases: AI326936; Bp50; Cd40; GP39; HIGM1; IGM; IMD3; p50; T-BAM; Tnfrsf5; TRAP

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UniProt ID: (Mouse) Q99NE2

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Entrez Gene ID: (Rat) 171369, (Mouse) 21939

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Function(s)

antigen binding protein binding enzyme binding ubiquitin protein ligase binding transmembrane signal receptor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD40 Monoclonal Antibody (HM40-3), eFluor™ 450, eBioscience™

CD40 Antibody (48-0402-82) in Flow

Product Details

48-0402-82

Flow Cytometry (Flow)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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