Invitrogen

Granzyme B Monoclonal Antibody (NGZB), eFluor™ 450, eBioscience™

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Tech Support

Antibody Testing Data (1)
Published Figures (39)

Granzyme B Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 40

Granzyme B Antibody (48-8898-82) in Flow

Balb/c splenocytes were cultured with plate-bound Anti-Mouse CD3e Functional Grade Purified (Product # 16-0031-82) and Anti-Mouse CD28 Functional Grade Purified (Product # 16-0281-82) for 3 days, then cultured with Protein Transport Inhibitor Cocktail (Product # 00-4980-03) for an additional 5 hours. Cells were surface stained with Anti-Mouse CD8a PE (Product # 12-0081-82) followed by intracellular staining with 0.06 µg of Rat IgG2a K Isotype Control eFluor® 450 (Product # 48-4321-82) (left) or 0.06 µg of Anti-Mouse Granzyme B eFluor® 450 ... View More

Granzyme B Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

48-8898-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Flow Cytometry (Flow)

0.125 µg/test

View 43 publications 43 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

NGZB

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11149362

Product Specific Information

Description: This NGZB monoclonal antibody reacts with mouse Granzyme B, which is a member of the granzyme serine protease family. Granzyme B is found in the granules of cytotoxic T cells and NK cells. Granzyme B has also been described as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation, and CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic T cells, but not observed in non-cytotoxic lymphoid cells. Granzyme B is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells. The receptor involved has been identified as mannose 6-phosphate receptor. This receptor functions as a death receptor for Granzyme B during cytotoxic T cell-induced apoptosis. This NGZB monoclonal antibody does not crossreact to human Granzyme B nor is staining blocked with GB11, suggesting it recognizes a different epitope.

Applications Reported: This NGZB antibody has been reported for use in intracellular staining and flow cytometric analysis.

Applications Tested: This NGZB antibody has been tested by intracellular staining followed by flow cytometric analysis of stimulated mouse splenocytes using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Granzyme B is a member of the granzyme serine protease family, and is found in the granules of cytotoxic T cells and NK cells. Granzyme B has been described as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation, and CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic T cells, but not observed in non-cytotoxic lymphoid cells. Granzyme B is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells. The receptor involved in this process has been identified as mannose 6-phosphate receptor which functions as a death receptor for Granzyme B during cytotoxic T cell-induced apoptosis. Granzyme B enters target cells to cleave caspase-3 and initiate the caspase cascade leading to DNA fragmentation and apoptosis. Granzyme B can also act through a mitochondrial apoptosis pathway by cleaving the Bid protein. Granzymes are neutral serine proteases, which are stored in specialized lytic granules of cytotoxic T lymphocytes (CTLs) and in natural killer (NK) cells. A number of granzymes (A to G) have been isolated and cloned from mouse CTLs and NK cells, however in man, fewer have been cloned and identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapy.

Nature communications

Xiao X,Shi J,He C,Bu X,Sun Y,Gao M,Xiang B,Xiong W,Dai P,Mao Q,Xing X,Yao Y,Yu H,Xu G,Li S,Ren Y,Chen B,Jiang C,Meng G,Lee YR,Wei W,Freeman GJ,Xie C,Zhang J

Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry

Fri May 19 00:00:00 EDT 2023

Small-molecule PTPN2 Inhibitors Sensitize Resistant Melanoma to Anti-PD-1 Immunotherapy.

Cancer research communications

Zhu Z,Tang R,Huff S,Kummetha IR,Wang L,Li N,Rana TM

Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry

Sun Jan 01 00:00:00 EST 2023

The altering cellular components and function in tumor microenvironment during remissive and relapsed stages of anti-CD19 CAR T-cell treated lymphoma mice.

Frontiers in immunology

Zhao K,Ren C,Tang D,Zhao L,Chen X,Wang Y,Xu K

48-8898-82 was used in Flow cytometry/Cell sorting to find that anti-CD19 CAR T-cell treatment attenuated the symptoms of lymphoma and significantly prolonged mice survival through eradicating systemic CD19+ cells.

Mon Feb 13 00:00:00 EST 2023

Histone methyltransferase WHSC1 loss dampens MHC-I antigen presentation pathway to impair IFN-γ-stimulated antitumor immunity.

The Journal of clinical investigation

Ren J,Li N,Pei S,Lian Y,Li L,Peng Y,Liu Q,Guo J,Wang X,Han Y,Zhang G,Wang H,Li Y,Jiang J,Li Q,Tan M,Peng J,Hu G,Xiao Y,Li X,Lin M,Qin J

Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry

Fri Apr 15 00:00:00 EDT 2022

Targeting Fc Receptor-Mediated Effects and the "Don't Eat Me" Signal with an Oncolytic Virus Expressing an Anti-CD47 Antibody to Treat Metastatic Ovarian Cancer.

Clinical cancer research : an official journal of the American Association for Cancer Research

Tian L,Xu B,Teng KY,Song M,Zhu Z,Chen Y,Wang J,Zhang J,Feng M,Kaur B,Rodriguez L,Caligiuri MA,Yu J

48-8898-82 was used in Flow cytometry/Cell sorting to improve therapeutic efficacy while reducing toxicities for ovarian cancer.

Sat Jan 01 00:00:00 EST 2022

Wnt5A Signaling Blocks Progression of Experimental Visceral Leishmaniasis.

Frontiers in immunology

Maity S,Chakraborty A,Mahata SK,Roy S,Das AK,Sen M

Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry

Tue Mar 22 00:00:00 EDT 2022

Intranasal Delivery of Recombinant S100A8 Protein Delays Lung Cancer Growth by Remodeling the Lung Immune Microenvironment.

Frontiers in immunology

Wong SW,McCarroll J,Hsu K,Geczy CL,Tedla N

Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry

Mon Jun 06 00:00:00 EDT 2022

eEF2K promotes PD-L1 stabilization through inactivating GSK3β in melanoma.

Journal for immunotherapy of cancer

Chen X,Wang K,Jiang S,Sun H,Che X,Zhang M,He J,Wen Y,Liao M,Li X,Zhou X,Song J,Ren X,Yi W,Yang J,Chen X,Yin M,Cheng Y

Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Immunohistochemistry

Tue Mar 01 00:00:00 EST 2022

eEF2K promotes PD-L1 stabilization through inactivating GSK3β in melanoma.

Journal for immunotherapy of cancer

Chen X,Wang K,Jiang S,Sun H,Che X,Zhang M,He J,Wen Y,Liao M,Li X,Zhou X,Song J,Ren X,Yi W,Yang J,Chen X,Yin M,Cheng Y

Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Immunohistochemistry

Tue Mar 01 00:00:00 EST 2022

RAD21 amplification epigenetically suppresses interferon signaling to promote immune evasion in ovarian cancer.

The Journal of clinical investigation

Deng P,Wang Z,Chen J,Liu S,Yao X,Liu S,Liu L,Yu Z,Huang Y,Xiong Z,Xiao R,Gao J,Liang W,Chen J,Liu H,Hong JH,Chan JY,Guan P,Chen J,Wang Y,Yin J,Li J,Zheng M,Zhang C,Zhou P,Kang T,Teh BT,Yu Q,Zuo Z,Jiang Q,Liu J,Xiong Y,Xia X,Tan J

Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry

Tue Nov 15 00:00:00 EST 2022

PRDM1/BLIMP1 induces cancer immune evasion by modulating the USP22-SPI1-PD-L1 axis in hepatocellular carcinoma cells.

Nature communications

Li Q,Zhang L,You W,Xu J,Dai J,Hua D,Zhang R,Yao F,Zhou S,Huang W,Dai Y,Zhang Y,Baheti T,Qian X,Pu L,Xu J,Xia Y,Zhang C,Tang J,Wang X

Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry

Mon Dec 12 00:00:00 EST 2022

PRDM1/BLIMP1 induces cancer immune evasion by modulating the USP22-SPI1-PD-L1 axis in hepatocellular carcinoma cells.

Nature communications

Li Q,Zhang L,You W,Xu J,Dai J,Hua D,Zhang R,Yao F,Zhou S,Huang W,Dai Y,Zhang Y,Baheti T,Qian X,Pu L,Xu J,Xia Y,Zhang C,Tang J,Wang X

Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry

Mon Dec 12 00:00:00 EST 2022

Mobilizing phospholipids on tumor plasma membrane implicates phosphatidylserine externalization blockade for cancer immunotherapy.

Cell reports

Wang W,Wu S,Cen Z,Zhang Y,Chen Y,Huang Y,Cillo AR,Prokopec JS,Quarato G,Vignali DAA,Stewart-Ornstein J,Li S,Lu B,Gong YN

Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry

Tue Nov 01 00:00:00 EDT 2022

Specific targeting of glioblastoma with an oncolytic virus expressing a cetuximab-CCL5 fusion protein via innate and adaptive immunity.

Nature cancer

Tian L,Xu B,Chen Y,Li Z,Wang J,Zhang J,Ma R,Cao S,Hu W,Chiocca EA,Kaur B,Caligiuri MA,Yu J

48-8898-82 was used in Flow cytometry/Cell sorting to demonstrate that OV-Cmab-CCL5 offers a promising approach to improve OV therapy for solid tumors.

Tue Nov 01 00:00:00 EDT 2022

CD36-mediated ferroptosis dampens intratumoral CD8⁺ T cell effector function and impairs their antitumor ability.

Cell metabolism

Ma X,Xiao L,Liu L,Ye L,Su P,Bi E,Wang Q,Yang M,Qian J,Yi Q

48-8898-82 was used in Flow cytometry/Cell sorting to reveal a new mechanism of CD36 regulating the function of CD8+ effector T cells and therapeutic potential of targeting CD36 or inhibiting ferroptosis to restore T cell function.

Tue May 04 00:00:00 EDT 2021

Blockade of 6-phosphogluconate dehydrogenase generates CD8⁺ effector T cells with enhanced anti-tumor function.

Cell reports

Daneshmandi S,Cassel T,Lin P,Higashi RM,Wulf GM,Boussiotis VA,Fan TW,Seth P

Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry

Tue Mar 09 00:00:00 EST 2021

Integrin αvβ8 on T cells suppresses anti-tumor immunity in multiple models and is a promising target for tumor immunotherapy.

Cell reports

Dodagatta-Marri E,Ma HY,Liang B,Li J,Meyer DS,Chen SY,Sun KH,Ren X,Zivak B,Rosenblum MD,Headley MB,Pinzas L,Reed NI,Del Cid JS,Hann BC,Yang S,Giddabasappa A,Noorbehesht K,Yang B,Dal Porto J,Tsukui T,Niessen K,Atakilit A,Akhurst RJ,Sheppard D

Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry

Tue Jul 06 00:00:00 EDT 2021

Aiduqing formula inhibits breast cancer metastasis by suppressing TAM/CXCL1-induced Treg differentiation and infiltration.

Cell communication and signaling : CCS

Li J,Wang S,Wang N,Zheng Y,Yang B,Wang X,Zhang J,Pan B,Wang Z

Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry

Mon Aug 30 00:00:00 EDT 2021

Extracellular Vesicles from Akkermansia muciniphila Elicit Antitumor Immunity Against Prostate Cancer via Modulation of CD8⁺ T Cells and Macrophages.

International journal of nanomedicine

Luo ZW,Xia K,Liu YW,Liu JH,Rao SS,Hu XK,Chen CY,Xu R,Wang ZX,Xie H

Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry

Tue Jun 01 00:00:00 EDT 2021

Non-redundant activity of GSK-3α and GSK-3β in T cell-mediated tumor rejection.

iScience

Steele L,Mannion AJ,Shaw G,Maclennan KA,Cook GP,Rudd CE,Taylor A

Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry

Fri Jun 25 00:00:00 EDT 2021

Circadian Clock Disruption Suppresses PDL1⁺ Intraepithelial B Cells in Experimental Colitis and Colitis-Associated Colorectal Cancer.

Cellular and molecular gastroenterology and hepatology

Liu JL,Wang CY,Cheng TY,Rixiati Y,Ji C,Deng M,Yao S,Yuan LH,Zhao YY,Shen T,Li JM

Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry

Mon Jan 24 00:00:00 EST 2022

A booster dose enhances immunogenicity of the COVID-19 vaccine candidate ChAdOx1 nCoV-19 in aged mice.

Med (New York, N.Y.)

Silva-Cayetano A,Foster WS,Innocentin S,Belij-Rammerstorfer S,Spencer AJ,Burton OT,Fra-Bidó S,Le Lee J,Thakur N,Conceicao C,Wright D,Barrett J,Evans-Bailey N,Noble C,Bailey D,Liston A,Gilbert SC,Lambe T,Linterman MA

48-8898-82 was used in Flow Cytometry to show that ChAdOx1 nCoV-19 induces both cellular and humoral immunity in adult and aged mice and suggest a prime-boost strategy is a rational approach to enhance immunogenicity in older persons.

Fri Mar 12 00:00:00 EST 2021

Knockout of immunotherapy prognostic marker genes eliminates the effect of the anti-PD-1 treatment.

NPJ precision oncology

Yang N,Ji F,Cheng L,Lu J,Sun X,Lin X,Lan X

Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry

Fri May 07 00:00:00 EDT 2021

Knockout of immunotherapy prognostic marker genes eliminates the effect of the anti-PD-1 treatment.

NPJ precision oncology

Yang N,Ji F,Cheng L,Lu J,Sun X,Lin X,Lan X

Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry

Fri May 07 00:00:00 EDT 2021

Modulation of lactate-lysosome axis in dendritic cells by clotrimazole potentiates antitumor immunity.

Journal for immunotherapy of cancer

Wang Z,Xu F,Hu J,Zhang H,Cui L,Lu W,He W,Wang X,Li M,Zhang H,Xiong W,Xie C,Liu Y,Zhou P,Liu J,Huang P,Qin XF,Xia X

48-8898-82 was used in Flow Cytometry to show that clotrimazole could trigger DC activation via the lactate-lysosome axis to promote antigen cross-presentation and could be used as a potential combination therapy approach to improving the therapeutic efficacy of anti-PD1 immunotherapy.

Sat May 01 00:00:00 EDT 2021

Identification of HLA-A2-Restricted Mutant Epitopes from Neoantigens of Esophageal Squamous Cell Carcinoma.

Vaccines

Wang Z,Ran L,Chen C,Shi R,Dong Y,Li Y,Zhou X,Qi Y,Zhu P,Gao Y,Wu Y

Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Protein Assays and Analysis

Fri Oct 01 00:00:00 EDT 2021

Fluvastatin-Pretreated Donor Cells Attenuated Murine aGVHD by Balancing Effector T Cell Distribution and Function under the Regulation of KLF2.

BioMed research international

Zhao K,Tian Y,Wang J,Chen C,Pan B,Yan Z,Zhu S,Xu K

48-8898 was used in Flow cytometry/Cell sorting to conclude that administration of Fluvastatin to donor mice showed protective effects against recipient aGVHD when compared to untreated mice due to the retention of effector T cells in lymphoid organs accompanying with reduction of nonlymphatic infiltration and related inflammatory cytokines.

Fri Sep 10 00:00:00 EDT 2021

Assessment of the WAP-Myc mouse mammary tumor model for spontaneous metastasis.

Scientific reports

Utz B,Turpin R,Lampe J,Pouwels J,Klefström J

Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry

Fri Oct 30 00:00:00 EDT 2020

Cancer-Specific Loss of p53 Leads to a Modulation of Myeloid and T Cell Responses.

Cell reports

Blagih J,Zani F,Chakravarty P,Hennequart M,Pilley S,Hobor S,Hock AK,Walton JB,Morton JP,Gronroos E,Mason S,Yang M,McNeish I,Swanton C,Blyth K,Vousden KH

Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry

Tue Jan 14 00:00:00 EST 2020

Salmonella-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Restructures the Immune Contexture to Improve Checkpoint Blockade Efficacy.

Biomedicines

Ebelt ND,Zuniga E,Marzagalli M,Zamloot V,Blazar BR,Salgia R,Manuel ER

Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry

Wed Dec 16 00:00:00 EST 2020

Thymic iNKT single cell analyses unmask the common developmental program of mouse innate T cells.

Nature communications

Harsha Krovi S,Zhang J,Michaels-Foster MJ,Brunetti T,Loh L,Scott-Browne J,Gapin L

48-8898 was used in Flow cytometry/Cell sorting to highlight the common requirements for the development of innate-like T cells with a focus on how Hivep3 impacts the maturation of these lymphocytes.

Mon Dec 07 00:00:00 EST 2020

Memory CD73+IgM+ B cells protect against Plasmodium yoelii infection and express Granzyme B.

PloS one

Parra M,Weitner M,Yang A,Akue A,Liu X,Schmidt T,Allman WR,Akkoyunlu M,Derrick SC

Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry

Tue Oct 27 00:00:00 EDT 2020

cGAS/STING axis mediates a topoisomerase II inhibitor-induced tumor immunogenicity.

The Journal of clinical investigation

Wang Z,Chen J,Hu J,Zhang H,Xu F,He W,Wang X,Li M,Lu W,Zeng G,Zhou P,Huang P,Chen S,Li W,Xia LP,Xia X

48-8898 was used in Flow cytometry/Cell sorting to show that teniposide could trigger tumor immunogenicity and enable a potential chemoimmunotherapeutic approach to potentiating the therapeutic efficacy of anti-PD1 immunotherapy.

Tue Aug 13 00:00:00 EDT 2019

Inflammation induced by incomplete radiofrequency ablation accelerates tumor progression and hinders PD-1 immunotherapy.

Nature communications

Shi L,Wang J,Ding N,Zhang Y,Zhu Y,Dong S,Wang X,Peng C,Zhou C,Zhou L,Li X,Shi H,Wu W,Long X,Wu C,Liao W

Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry

Thu Nov 28 00:00:00 EST 2019

A Macrophage Colony-Stimulating-Factor-Producing γδ T Cell Subset Prevents Malarial Parasitemic Recurrence.

Immunity

Mamedov MR,Scholzen A,Nair RV,Cumnock K,Kenkel JA,Oliveira JHM,Trujillo DL,Saligrama N,Zhang Y,Rubelt F,Schneider DS,Chien YH,Sauerwein RW,Davis MM

48-8898-82 was used in Flow Cytometry to point to an M-CSF-producing γδ T cell subset that fulfills a specialized protective role in the later stage of malaria infection when αβ T cells have declined.

Tue Feb 20 00:00:00 EST 2018

MAIT cells contribute to protection against lethal influenza infection in vivo.

Nature communications

van Wilgenburg B,Loh L,Chen Z,Pediongco TJ,Wang H,Shi M,Zhao Z,Koutsakos M,Nüssing S,Sant S,Wang Z,D'Souza C,Jia X,Almeida CF,Kostenko L,Eckle SBG,Meehan BS,Kallies A,Godfrey DI,Reading PC,Corbett AJ,McCluskey J,Klenerman P,Kedzierska K,Hinks TSC

Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry

Fri Nov 09 00:00:00 EST 2018

Crosstalks between mTORC1 and mTORC2 variagate cytokine signaling to control NK maturation and effector function.

Nature communications

Wang F,Meng M,Mo B,Yang Y,Ji Y,Huang P,Lai W,Pan X,You T,Luo H,Guan X,Deng Y,Yuan S,Chu J,Namaka M,Hughes T,Ye L,Yu J,Li X,Deng Y

Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry

Mon Nov 19 00:00:00 EST 2018

Regulation of chitinase-3-like-1 in T cell elicits Th1 and cytotoxic responses to inhibit lung metastasis.

Nature communications

Kim DH,Park HJ,Lim S,Koo JH,Lee HG,Choi JO,Oh JH,Ha SJ,Kang MJ,Lee CM,Lee CG,Elias JA,Choi JM

Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry

Mon Feb 05 00:00:00 EST 2018

The immunocytokine L19-IL2: An interplay between radiotherapy and long-lasting systemic anti-tumour immune responses.

Oncoimmunology

Rekers NH,Olivo Pimentel V,Yaromina A,Lieuwes NG,Biemans R,Zegers CML,Germeraad WTV,Van Limbergen EJ,Neri D,Dubois LJ,Lambin P

48-8898 was used in Flow cytometry/Cell sorting to show that radiotherapy and L19-IL2 can activate a curative abscopal effect, with a long-lasting immunological memory.

Tue Jan 12 00:00:00 EST 2021

Combined antiangiogenic and anti-PD-L1 therapy stimulates tumor immunity through HEV formation.

Science translational medicine

Allen E,Jabouille A,Rivera LB,Lodewijckx I,Missiaen R,Steri V,Feyen K,Tawney J,Hanahan D,Michael IP,Bergers G

Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry

Wed Apr 12 00:00:00 EDT 2017

Egr2 and 3 control adaptive immune responses by temporally uncoupling expansion from T cell differentiation.

The Journal of experimental medicine

Miao T,Symonds ALJ,Singh R,Symonds JD,Ogbe A,Omodho B,Zhu B,Li S,Wang P

Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry

Mon Jun 05 00:00:00 EDT 2017

1810011o10 Rik Inhibits the Antitumor Effect of Intratumoral CD8⁺ T Cells through Suppression of Notch2 Pathway in a Murine Hepatocellular Carcinoma Model.

Frontiers in immunology

Dai K,Huang L,Huang YB,Chen ZB,Yang LH,Jiang YA

Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry

Wed Nov 20 00:00:00 EST 2019

Loss of HIF-1α in natural killer cells inhibits tumour growth by stimulating non-productive angiogenesis.

Nature communications

Krzywinska E,Kantari-Mimoun C,Kerdiles Y,Sobecki M,Isagawa T,Gotthardt D,Castells M,Haubold J,Millien C,Viel T,Tavitian B,Takeda N,Fandrey J,Vivier E,Sexl V,Stockmann C

Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry

Fri Nov 17 00:00:00 EST 2017

The stress kinase GCN2 does not mediate suppression of antitumor T cell responses by tryptophan catabolism in experimental melanomas.

Oncoimmunology

Sonner JK,Deumelandt K,Ott M,Thomé CM,Rauschenbach KJ,Schulz S,Munteanu B,Mohapatra S,Adam I,Hofer AC,Feuerer M,Opitz CA,Hopf C,Wick W,Platten M

Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Caribbean and La Réunion Chikungunya Virus Isolates Differ in Their Capacity To Induce Proinflammatory Th1 and NK Cell Responses and Acute Joint Pathology.

Journal of virology

Teo TH,Her Z,Tan JJ,Lum FM,Lee WW,Chan YH,Ong RY,Kam YW,Leparc-Goffart I,Gallian P,Rénia L,de Lamballerie X,Ng LF

Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2015

Caribbean and La Réunion Chikungunya Virus Isolates Differ in Their Capacity To Induce Proinflammatory Th1 and NK Cell Responses and Acute Joint Pathology.

Journal of virology

Teo TH,Her Z,Tan JJ,Lum FM,Lee WW,Chan YH,Ong RY,Kam YW,Leparc-Goffart I,Gallian P,Rénia L,de Lamballerie X,Ng LF

Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2015

Metastasis is regulated via microRNA-200/ZEB1 axis control of tumour cell PD-L1 expression and intratumoral immunosuppression.

Nature communications

Chen L,Gibbons DL,Goswami S,Cortez MA,Ahn YH,Byers LA,Zhang X,Yi X,Dwyer D,Lin W,Diao L,Wang J,Roybal J,Patel M,Ungewiss C,Peng D,Antonia S,Mediavilla-Varela M,Robertson G,Suraokar M,Welsh JW,Erez B,Wistuba II,Chen L,Peng D,Wang S,Ullrich SE,Heymach JV,Kurie JM,Qin FX

Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry

Tue Oct 28 00:00:00 EDT 2014

Tim-3 negatively mediates natural killer cell function in LPS-induced endotoxic shock.

PloS one

Hou H,Liu W,Wu S,Lu Y,Peng J,Zhu Y,Lu Y,Wang F,Sun Z

Published figure using Granzyme B monoclonal antibody (Product # 48-8898-82) in Flow Cytometry

Mon Jul 06 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: CCP1; CTLA-1; Cytotoxic cell protease 1; Cytotoxic T lymphocyte associated serine esterase 1; Fragmentin-2; Granzyme B(G,H); GranzymeB; Human lymphocyte protein (Hlp); OTTHUMP00000028189

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Gene Aliases: AI553453; CCP-1/C11; CCP1; Ctla-1; Ctla1; GZB; Gzmb

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UniProt ID: (Mouse) P04187

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Entrez Gene ID: (Mouse) 14939

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Function(s)

serine-type endopeptidase activity protein binding peptidase activity serine-type peptidase activity hydrolase activity protease serine protease protein modifying enzyme

Process(es)

T cell mediated cytotoxicity proteolysis apoptotic process granzyme-mediated apoptotic signaling pathway protein processing cytolysis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

Granzyme B Monoclonal Antibody (NGZB), eFluor™ 450, eBioscience™

Granzyme B Antibody (48-8898-82) in Flow

Product Details

48-8898-82

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

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Bioinformatics

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