Invitrogen

CD19 Monoclonal Antibody (eBio1D3 (1D3)), eFluor™ 660, eBioscience™

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CD19 Antibody (50-0193-82) in Flow

Staining of BALB/c splenocytes with Anti-Mouse CD3e FITC (Product # 11-0031-82) and 0.125 µg of Rat IgG2a K Isotype Control eFluor® 660 (Product # 50-4321-82) (left) or 0.125 µg of Anti-Mouse CD19 eFluor® 660 (right). Total viable cells were used for analysis.

CD19 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

50-0193-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.25 µg/test

View 52 publications 52 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 660, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBio1D3 (1D3)

Conjugate

eFluor™ 660 eFluor™ 660 eFluor™ 660

Excitation/Emission Max

651/668 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11218286

Product Specific Information

Description: The eBio1D3 (1D3) monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells.

Applications Reported: This eBio1D3 (1D3) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBio1D3 (1D3) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of kappa or lambda light chains. CD19 appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen. The receptor for CD19 is an important functional regulator of normal and malignant B cell proliferation, and is expressed in all B cell precursor leukemias. Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. CD19 is a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. Besides being a signal-amplifying coreceptor for the B cell receptor (BCR), CD19 can also signal independently of BCR co-ligation and is a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

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Wed Jan 04 00:00:00 EST 2023

Role of FGF10/FGFR2b Signaling in Homeostasis and Regeneration of Adult Lacrimal Gland and Corneal Epithelium Proliferation.

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Tue Jan 03 00:00:00 EST 2023

High-yield enrichment of mouse small intestine intraepithelial lymphocytes by immunomagnetic depletion of EpCAM⁺ cells.

STAR protocols

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High-yield enrichment of mouse small intestine intraepithelial lymphocytes by immunomagnetic depletion of EpCAM⁺ cells.

STAR protocols

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Fri Mar 18 00:00:00 EDT 2022

Single-cell transcriptomics reveals distinct cell response between acute and chronic pulmonary infection of Pseudomonas aeruginosa.

MedComm

Hu X,Wu M,Ma T,Zhang Y,Zou C,Wang R,Zhang Y,Ren Y,Li Q,Liu H,Li H,Wang T,Sun X,Yang Y,Tang M,Li X,Li J,Gao X,Li T,Zhou X

Published figure using CD19 monoclonal antibody (Product # 50-0193-82) in Immunohistochemistry

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Valosin-containing protein (VCP/p97) inhibition reduces viral clearance and induces toxicity associated with muscular damage.

Cell death & disease

Del Rio Oliva M,Basler M

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The ubiquitin ligase Peli1 inhibits ICOS and thereby Tfh-mediated immunity.

Cellular & molecular immunology

Huang X,Hao S,Liu J,Huang Y,Liu M,Xiao C,Wang Y,Pei S,Yu T,Xu J,Wang H,Dai D,Su X,Xiao Y

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Functional human IgA targets a conserved site on malaria sporozoites.

Science translational medicine

Tan J,Cho H,Pholcharee T,Pereira LS,Doumbo S,Doumtabe D,Flynn BJ,Schön A,Kanatani S,Aylor SO,Oyen D,Vistein R,Wang L,Dillon M,Skinner J,Peterson M,Li S,Idris AH,Molina-Cruz A,Zhao M,Olano LR,Lee PJ,Roth A,Sinnis P,Barillas-Mury C,Kayentao K,Ongoiba A,Francica JR,Traore B,Wilson IA,Seder RA,Crompton PD

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Chronic T cell proliferation in brains after stroke could interfere with the efficacy of immunotherapies.

The Journal of experimental medicine

Heindl S,Ricci A,Carofiglio O,Zhou Q,Arzberger T,Lenart N,Franzmeier N,Hortobagyi T,Nelson PT,Stowe AM,Denes A,Edbauer D,Liesz A

Published figure using CD19 monoclonal antibody (Product # 50-0193-82) in Flow Cytometry

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Aged hematopoietic stem cells are refractory to bloodborne systemic rejuvenation interventions.

The Journal of experimental medicine

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Published figure using CD19 monoclonal antibody (Product # 50-0193-82) in Flow Cytometry

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The gammaherpesvirus 68 viral cyclin facilitates expression of LANA.

PLoS pathogens

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Published figure using CD19 monoclonal antibody (Product # 50-0193-82) in Flow Cytometry

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STING Activated Tumor-Intrinsic Type I Interferon Signaling Promotes CXCR3 Dependent Antitumor Immunity in Pancreatic Cancer.

Cellular and molecular gastroenterology and hepatology

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Published figure using CD19 monoclonal antibody (Product # 50-0193-82) in Flow Cytometry

Mon Jan 24 00:00:00 EST 2022

Regulatory B Cells (Bregs) Inhibit Osteoclastogenesis and Play a Potential Role in Ameliorating Ovariectomy-Induced Bone Loss.

Frontiers in immunology

Sapra L,Bhardwaj A,Mishra PK,Garg B,Verma B,Mishra GC,Srivastava RK

Published figure using CD19 monoclonal antibody (Product # 50-0193-82) in Flow Cytometry

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Comprehensive mutagenesis identifies the peptide repertoire of a p53 T-cell receptor mimic antibody that displays no toxicity in mice transgenic for human HLA-A*0201.

PloS one

Trenevska I,Anderson AP,Bentley C,Hassanali T,Wiblin S,Maguire S,Pezzella F,Banham AH,Li D

Published figure using CD19 monoclonal antibody (Product # 50-0193-82) in Flow Cytometry

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Calibration of cell-intrinsic interleukin-2 response thresholds guides design of a regulatory T cell biased agonist.

eLife

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Excessive Costimulation Leads to Dysfunction of Adoptively Transferred T Cells.

Cancer immunology research

Wijewarnasuriya D,Bebernitz C,Lopez AV,Rafiq S,Brentjens RJ

Published figure using CD19 monoclonal antibody (Product # 50-0193-82) in Flow Cytometry

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Differentiation Potential of Early- and Late-Passage Adipose-Derived Mesenchymal Stem Cells Cultured under Hypoxia and Normoxia.

Stem cells international

Zhao AG,Shah K,Freitag J,Cromer B,Sumer H

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Tue Oct 06 00:00:00 EDT 2020

Zdhhc2 Is Essential for Plasmacytoid Dendritic Cells Mediated Inflammatory Response in Psoriasis.

Frontiers in immunology

Zhou B,Yang W,Li W,He L,Lu L,Zhang L,Liu Z,Wang Y,Chao T,Huang R,Gu Y,Jia T,Liu Q,Tian S,Pierre P,Maeda T,Liang Y,Kong E

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Wed Jun 23 00:00:00 EDT 2021

CNS Immune Profiling in a Dengue Virus-Infected Immunocompetent Outbred ICR Mice Strain.

Frontiers in cellular and infection microbiology

Shen TJ,Chen CL,Jhan MK,Tseng PC,Lin CF

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Thu Jun 17 00:00:00 EDT 2021

Memory CD73+IgM+ B cells protect against Plasmodium yoelii infection and express Granzyme B.

PloS one

Parra M,Weitner M,Yang A,Akue A,Liu X,Schmidt T,Allman WR,Akkoyunlu M,Derrick SC

Published figure using CD19 monoclonal antibody (Product # 50-0193-82) in Flow Cytometry

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Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry.

STAR protocols

Liu Z,Gu Y,Shin A,Zhang S,Ginhoux F

Published figure using CD19 monoclonal antibody (Product # 50-0193-82) in Flow Cytometry

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Tregitopes regulate the tolerogenic immune response and decrease the foetal death rate in abortion-prone mouse matings.

Scientific reports

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Frontiers in immunology

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Variation in zygotic CRISPR/Cas9 gene editing outcomes generates novel reporter and deletion alleles at the Gdf11 locus.

Scientific reports

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Mon Dec 09 00:00:00 EST 2019

Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells.

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Published figure using CD19 monoclonal antibody (Product # 50-0193-82) in Flow Cytometry

Thu Jan 25 00:00:00 EST 2018

TET2 Deficiency Causes Germinal Center Hyperplasia, Impairs Plasma Cell Differentiation, and Promotes B-cell Lymphomagenesis.

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Dominguez PM,Ghamlouch H,Rosikiewicz W,Kumar P,Béguelin W,Fontán L,Rivas MA,Pawlikowska P,Armand M,Mouly E,Torres-Martin M,Doane AS,Calvo Fernandez MT,Durant M,Della-Valle V,Teater M,Cimmino L,Droin N,Tadros S,Motanagh S,Shih AH,Rubin MA,Tam W,Aifantis I,Levine RL,Elemento O,Inghirami G,Green MR,Figueroa ME,Bernard OA,Aoufouchi S,Li S,Shaknovich R,Melnick AM

Published figure using CD19 monoclonal antibody (Product # 50-0193-82) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2018

11 Beta-hydroxysteroid dehydrogenase type 1 regulates synovitis, joint destruction, and systemic bone loss in chronic polyarthritis.

Journal of autoimmunity

Hardy RS,Fenton C,Croft AP,Naylor AJ,Begum R,Desanti G,Buckley CD,Lavery G,Cooper MS,Raza K

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Inactivation of mTORC1 Signaling in Osterix-Expressing Cells Impairs B-cell Differentiation.

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Wang Y,Xiao M,Tao C,Chen J,Wang Z,Yang J,Chen Z,Zou Z,Liu A,Cai D,Jiang Y,Ding C,Li M,Bai X

Published figure using CD19 monoclonal antibody (Product # 50-0193-82) in Flow Cytometry

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The Role of Allograft Inflammatory Factor-1 in the Effects of Experimental Diabetes on B Cell Functions in the Heart.

Frontiers in cardiovascular medicine

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In Vitro Differentiation of Gata2 and Ly6a Reporter Embryonic Stem Cells Corresponds to In Vivo Waves of Hematopoietic Cell Generation.

Stem cell reports

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Peli1 negatively regulates noncanonical NF-κB signaling to restrain systemic lupus erythematosus.

Nature communications

Liu J,Huang X,Hao S,Wang Y,Liu M,Xu J,Zhang X,Yu T,Gan S,Dai D,Luo X,Lu Q,Mao C,Zhang Y,Shen N,Li B,Huang M,Zhu X,Jin J,Cheng X,Sun SC,Xiao Y

Published figure using CD19 monoclonal antibody (Product # 50-0193-82) in Flow Cytometry

Mon Mar 19 00:00:00 EDT 2018

Differential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus.

eLife

Moretti FA,Klapproth S,Ruppert R,Margraf A,Weber J,Pick R,Scheiermann C,Sperandio M,Fässler R,Moser M

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Thu Sep 06 00:00:00 EDT 2018

A protocol for generating induced T cells by reprogramming B cells in vivo.

Cell regeneration (London, England)

Weng Q,Hu F,Zhang M,Dong Y,Lv C,Wang Y,Liu X,Wang J

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S1P Signalling Differentially Affects Migration of Peritoneal B Cell Populations In Vitro and Influences the Production of Intestinal IgA In Vivo.

International journal of molecular sciences

Kleinwort A,Lührs F,Heidecke CD,Lipp M,Schulze T

50-0193 was used in Flow cytometry/Cell sorting to study the function of S1P signalling in the third B cell lineage.

Mon Jan 29 00:00:00 EST 2018

A therapeutic T cell receptor mimic antibody targets tumor-associated PRAME peptide/HLA-I antigens.

The Journal of clinical investigation

Chang AY,Dao T,Gejman RS,Jarvis CA,Scott A,Dubrovsky L,Mathias MD,Korontsvit T,Zakhaleva V,Curcio M,Hendrickson RC,Liu C,Scheinberg DA

Published figure using CD19 monoclonal antibody (Product # 50-0193-82) in Flow Cytometry

Fri Jun 30 00:00:00 EDT 2017

Prolonged Deleterious Influences of Chemotherapeutic Agent CPT-11 on Resident Peritoneal Macrophages and B1 Cells.

Frontiers in immunology

Bai WJ,Li CG,Zhang CC,Xu LH,Zeng QZ,Hu B,Hong Z,He XH,Ouyang DY

Published figure using CD19 monoclonal antibody (Product # 50-0193-82) in Flow Cytometry

Wed Nov 20 00:00:00 EST 2019

ADAR1 deletion induces NFκB and interferon signaling dependent liver inflammation and fibrosis.

RNA biology

Ben-Shoshan SO,Kagan P,Sultan M,Barabash Z,Dor C,Jacob-Hirsch J,Harmelin A,Pappo O,Marcu-Malina V,Ben-Ari Z,Amariglio N,Rechavi G,Goldstein I,Safran M

Published figure using CD19 monoclonal antibody (Product # 50-0193-82) in Flow Cytometry

Thu May 04 00:00:00 EDT 2017

Diminished bone regeneration after debridement of posttraumatic osteomyelitis is accompanied by altered cytokine levels, elevated B cell activity, and increased osteoclast activity.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society

Wagner JM,Jaurich H,Wallner C,Abraham S,Becerikli M,Dadras M,Harati K,Duhan V,Khairnar V,Lehnhardt M,Behr B

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Wed Nov 01 00:00:00 EDT 2017

Intravenously usable fully serotype 3 oncolytic adenovirus coding for CD40L as an enabler of dendritic cell therapy.

Oncoimmunology

Zafar S,Parviainen S,Siurala M,Hemminki O,Havunen R,Tähtinen S,Bramante S,Vassilev L,Wang H,Lieber A,Hemmi S,de Gruijl T,Kanerva A,Hemminki A

Published figure using CD19 monoclonal antibody (Product # 50-0193-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Extrafollicular CD4⁺ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

Nature communications

Deng R,Hurtz C,Song Q,Yue C,Xiao G,Yu H,Wu X,Muschen M,Forman S,Martin PJ,Zeng D

Published figure using CD19 monoclonal antibody (Product # 50-0193-82) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

Extrafollicular CD4⁺ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

Nature communications

Deng R,Hurtz C,Song Q,Yue C,Xiao G,Yu H,Wu X,Muschen M,Forman S,Martin PJ,Zeng D

Published figure using CD19 monoclonal antibody (Product # 50-0193-82) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

IL35-Producing B Cells Promote the Development of Pancreatic Neoplasia.

Cancer discovery

Pylayeva-Gupta Y,Das S,Handler JS,Hajdu CH,Coffre M,Koralov SB,Bar-Sagi D

Published figure using CD19 monoclonal antibody (Product # 50-0193-82) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2016

Medial HOXA genes demarcate haematopoietic stem cell fate during human development.

Nature cell biology

Dou DR,Calvanese V,Sierra MI,Nguyen AT,Minasian A,Saarikoski P,Sasidharan R,Ramirez CM,Zack JA,Crooks GM,Galic Z,Mikkola HK

Published figure using CD19 monoclonal antibody (Product # 50-0193-82) in Flow Cytometry

Wed Jun 01 00:00:00 EDT 2016

Molecular requirements of the B-cell antigen receptor for sensing monovalent antigens.

The EMBO journal

Volkmann C,Brings N,Becker M,Hobeika E,Yang J,Reth M

50-0193 was used in Flow cytometry/Cell sorting to study the effects of differing monovalent stimuli on B-cell antigen receptor signalling and organisation.

Wed Nov 02 00:00:00 EDT 2016

Antigen Targeting to Human HLA Class II Molecules Increases Efficacy of DNA Vaccination.

Journal of immunology (Baltimore, Md. : 1950)

Grodeland G,Fredriksen AB,Løset GÅ,Vikse E,Fugger L,Bogen B

Published figure using CD19 monoclonal antibody (Product # 50-0193-82) in Flow Cytometry

Tue Nov 01 00:00:00 EDT 2016

CD19 CAR immune pressure induces B-precursor acute lymphoblastic leukaemia lineage switch exposing inherent leukaemic plasticity.

Nature communications

Jacoby E,Nguyen SM,Fountaine TJ,Welp K,Gryder B,Qin H,Yang Y,Chien CD,Seif AE,Lei H,Song YK,Khan J,Lee DW,Mackall CL,Gardner RA,Jensen MC,Shern JF,Fry TJ

Published figure using CD19 monoclonal antibody (Product # 50-0193-82) in Flow Cytometry

Wed Jul 27 00:00:00 EDT 2016

Novel CXCL13 transgenic mouse: inflammation drives pathogenic effect of CXCL13 in experimental myasthenia gravis.

Oncotarget

Weiss JM,Robinet M,Aricha R,Cufi P,Villeret B,Lantner F,Shachar I,Fuchs S,Souroujon MC,Berrih-Aknin S,Le Panse R

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Tue Feb 16 00:00:00 EST 2016

Protein Kinase D2 Protects against Acute Colitis Induced by Dextran Sulfate Sodium in Mice.

Scientific reports

Xiong J,Zhou MF,Wang YD,Chen LP,Xu WF,Wang YD,Deng F,Liu SD

Published figure using CD19 monoclonal antibody (Product # 50-0193-82) in Flow Cytometry

Fri Sep 23 00:00:00 EDT 2016

Cis-regulatory mechanisms governing stem and progenitor cell transitions.

Science advances

Johnson KD,Kong G,Gao X,Chang YI,Hewitt KJ,Sanalkumar R,Prathibha R,Ranheim EA,Dewey CN,Zhang J,Bresnick EH

Published figure using CD19 monoclonal antibody (Product # 50-0193-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

Role of caveolin-3 in lymphocyte activation.

Life sciences

Tran C,Stary CM,Schilling JM,Bentley B,Patel HH,Roth DM

Published figure using CD19 monoclonal antibody (Product # 50-0193-82) in Flow Cytometry

Thu Jan 15 00:00:00 EST 2015

B cell Rab7 mediates induction of activation-induced cytidine deaminase expression and class-switching in T-dependent and T-independent antibody responses.

Journal of immunology (Baltimore, Md. : 1950)

Pone EJ,Lam T,Lou Z,Wang R,Chen Y,Liu D,Edinger AL,Xu Z,Casali P

Published figure using CD19 monoclonal antibody (Product # 50-0193-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2015

Mouse bone marrow-derived mesenchymal stem cells inhibit leukemia/lymphoma cell proliferation in vitro and in a mouse model of allogeneic bone marrow transplant.

International journal of molecular medicine

Song N,Gao L,Qiu H,Huang C,Cheng H,Zhou H,Lv S,Chen L,Wang J

Published figure using CD19 monoclonal antibody (Product # 50-0193-82) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2015

dNP2 is a blood-brain barrier-permeable peptide enabling ctCTLA-4 protein delivery to ameliorate experimental autoimmune encephalomyelitis.

Nature communications

Lim S,Kim WJ,Kim YH,Lee S,Koo JH,Lee JA,Yoon H,Kim DH,Park HJ,Kim HM,Lee HG,Yun Kim J,Lee JU,Hun Shin J,Kyun Kim L,Doh J,Kim H,Lee SK,Bothwell ALM,Suh M,Choi JM

Published figure using CD19 monoclonal antibody (Product # 50-0193-82) in Flow Cytometry

Tue Sep 15 00:00:00 EDT 2015

A novel method for autophagy detection in primary cells: impaired levels of macroautophagy in immunosenescent T cells.

Autophagy

Phadwal K,Alegre-Abarrategui J,Watson AS,Pike L,Anbalagan S,Hammond EM,Wade-Martins R,McMichael A,Klenerman P,Simon AK

Published figure using CD19 monoclonal antibody (Product # 50-0193-82) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2012

Critical role of B cell lymphoma 10 in BAFF-regulated NF-κB activation and survival of anergic B cells.

Journal of immunology (Baltimore, Md. : 1950)

Yu M,Chen Y,He Y,Podd A,Fu G,Wright JA,Kleiman E,Khan WN,Wen R,Wang D

Published figure using CD19 monoclonal antibody (Product # 50-0193-82) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2012

Bioinformatics

Protein Aliases: B-lymphocyte antigen CD19; CD19; Differentiation antigen CD19; Leu-12

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Gene Aliases: AW495831; Cd19

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UniProt ID: (Mouse) P25918

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Entrez Gene ID: (Mouse) 12478

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Process(es)

chromosome breakage B cell receptor signaling pathway positive regulation of release of sequestered calcium ion into cytosol

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CD19 Monoclonal Antibody (eBio1D3 (1D3)), eFluor™ 660, eBioscience™

CD19 Antibody (50-0193-82) in Flow

Product Details

50-0193-82

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

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