Invitrogen

CD19 Monoclonal Antibody (eBio1D3 (1D3)), PE-eFluor™ 610, eBioscience™

FIGURE: 1 / 64

CD19 Antibody (61-0193-82) in Flow

BALB/c mouse splenocytes were stained with CD3 Monoclonal Antibody, FITC (Product # 11-0031-82) and 0.125 µg of Rat IgG2a kappa Isotype Control, PE-eFluor 610 (Product # 61-4321-82) (left) or 0.125 µg of CD19 Monoclonal Antibody, PE-eFluor 610 (right). Total cells were used for analysis.

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Product Details

61-0193-82

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.25 µg/test

View 60 publications 60 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE-eFluor™ 610, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBio1D3 (1D3)

Conjugate

PE-eFluor™ 610 PE-eFluor™ 610 PE-eFluor™ 610

Excitation/Emission Max

565/606 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2574536

Product Specific Information

Description: The eBio1D3 (1D3) monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells.

Applications Reported: This eBio1D3 (1D3) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBio1D3 (1D3) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of kappa or lambda light chains. CD19 appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen. The receptor for CD19 is an important functional regulator of normal and malignant B cell proliferation, and is expressed in all B cell precursor leukemias. Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. CD19 is a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. Besides being a signal-amplifying coreceptor for the B cell receptor (BCR), CD19 can also signal independently of BCR co-ligation and is a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

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Published figure using CD19 monoclonal antibody (Product # 61-0193-82) in Flow Cytometry

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Functional human IgA targets a conserved site on malaria sporozoites.

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The Journal of experimental medicine

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Published figure using CD19 monoclonal antibody (Product # 61-0193-82) in Flow Cytometry

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Aged hematopoietic stem cells are refractory to bloodborne systemic rejuvenation interventions.

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The gammaherpesvirus 68 viral cyclin facilitates expression of LANA.

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Published figure using CD19 monoclonal antibody (Product # 61-0193-82) in Flow Cytometry

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Comprehensive mutagenesis identifies the peptide repertoire of a p53 T-cell receptor mimic antibody that displays no toxicity in mice transgenic for human HLA-A*0201.

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Published figure using CD19 monoclonal antibody (Product # 61-0193-82) in Flow Cytometry

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Excessive Costimulation Leads to Dysfunction of Adoptively Transferred T Cells.

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Published figure using CD19 monoclonal antibody (Product # 61-0193-82) in Flow Cytometry

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Zdhhc2 Is Essential for Plasmacytoid Dendritic Cells Mediated Inflammatory Response in Psoriasis.

Frontiers in immunology

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Published figure using CD19 monoclonal antibody (Product # 61-0193-82) in Flow Cytometry

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CNS Immune Profiling in a Dengue Virus-Infected Immunocompetent Outbred ICR Mice Strain.

Frontiers in cellular and infection microbiology

Shen TJ,Chen CL,Jhan MK,Tseng PC,Lin CF

Published figure using CD19 monoclonal antibody (Product # 61-0193-82) in Flow Cytometry

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Lipin-1 Contributes to IL-4 Mediated Macrophage Polarization.

Frontiers in immunology

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61-0193 was used in Flow cytometry/Cell sorting to provide evidence that lipin-1 transcriptional co-regulatory activity contributes to macrophage polarization and influences wound healing in vivo.

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Published figure using CD19 monoclonal antibody (Product # 61-0193-82) in Flow Cytometry

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Published figure using CD19 monoclonal antibody (Product # 61-0193-82) in Flow Cytometry

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Inactivation of mTORC1 Signaling in Osterix-Expressing Cells Impairs B-cell Differentiation.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Wang Y,Xiao M,Tao C,Chen J,Wang Z,Yang J,Chen Z,Zou Z,Liu A,Cai D,Jiang Y,Ding C,Li M,Bai X

Published figure using CD19 monoclonal antibody (Product # 61-0193-82) in Flow Cytometry

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The Role of Allograft Inflammatory Factor-1 in the Effects of Experimental Diabetes on B Cell Functions in the Heart.

Frontiers in cardiovascular medicine

Sarkar A,Shukla SK,Alqatawni A,Kumar A,Addya S,Tsygankov AY,Rafiq K

Published figure using CD19 monoclonal antibody (Product # 61-0193-82) in Flow Cytometry

Mon Dec 06 00:00:00 EST 2021

In Vitro Differentiation of Gata2 and Ly6a Reporter Embryonic Stem Cells Corresponds to In Vivo Waves of Hematopoietic Cell Generation.

Stem cell reports

Kauts ML,Rodriguez-Seoane C,Kaimakis P,Mendes SC,Cortés-Lavaud X,Hill U,Dzierzak E

Published figure using CD19 monoclonal antibody (Product # 61-0193-82) in Flow Cytometry

Tue Jan 09 00:00:00 EST 2018

Peli1 negatively regulates noncanonical NF-κB signaling to restrain systemic lupus erythematosus.

Nature communications

Liu J,Huang X,Hao S,Wang Y,Liu M,Xu J,Zhang X,Yu T,Gan S,Dai D,Luo X,Lu Q,Mao C,Zhang Y,Shen N,Li B,Huang M,Zhu X,Jin J,Cheng X,Sun SC,Xiao Y

Published figure using CD19 monoclonal antibody (Product # 61-0193-82) in Flow Cytometry

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Differential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus.

eLife

Moretti FA,Klapproth S,Ruppert R,Margraf A,Weber J,Pick R,Scheiermann C,Sperandio M,Fässler R,Moser M

Published figure using CD19 monoclonal antibody (Product # 61-0193-82) in Flow Cytometry

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A protocol for generating induced T cells by reprogramming B cells in vivo.

Cell regeneration (London, England)

Weng Q,Hu F,Zhang M,Dong Y,Lv C,Wang Y,Liu X,Wang J

Published figure using CD19 monoclonal antibody (Product # 61-0193-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

Intact interleukin-10 receptor signaling protects from hippocampal damage elicited by experimental neurotropic virus infection of SJL mice.

Scientific reports

Uhde AK,Ciurkiewicz M,Herder V,Khan MA,Hensel N,Claus P,Beckstette M,Teich R,Floess S,Baumgärtner W,Jung K,Huehn J,Beineke A

61-0193 was used in Flow cytometry/Cell sorting to study IL-10R signaling following virus-induced polioencephalitis in SJL mice.

Tue Apr 17 00:00:00 EDT 2018

A therapeutic T cell receptor mimic antibody targets tumor-associated PRAME peptide/HLA-I antigens.

The Journal of clinical investigation

Chang AY,Dao T,Gejman RS,Jarvis CA,Scott A,Dubrovsky L,Mathias MD,Korontsvit T,Zakhaleva V,Curcio M,Hendrickson RC,Liu C,Scheinberg DA

Published figure using CD19 monoclonal antibody (Product # 61-0193-82) in Flow Cytometry

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Prolonged Deleterious Influences of Chemotherapeutic Agent CPT-11 on Resident Peritoneal Macrophages and B1 Cells.

Frontiers in immunology

Bai WJ,Li CG,Zhang CC,Xu LH,Zeng QZ,Hu B,Hong Z,He XH,Ouyang DY

Published figure using CD19 monoclonal antibody (Product # 61-0193-82) in Flow Cytometry

Wed Nov 20 00:00:00 EST 2019

ADAR1 deletion induces NFκB and interferon signaling dependent liver inflammation and fibrosis.

RNA biology

Ben-Shoshan SO,Kagan P,Sultan M,Barabash Z,Dor C,Jacob-Hirsch J,Harmelin A,Pappo O,Marcu-Malina V,Ben-Ari Z,Amariglio N,Rechavi G,Goldstein I,Safran M

Published figure using CD19 monoclonal antibody (Product # 61-0193-82) in Flow Cytometry

Thu May 04 00:00:00 EDT 2017

Diminished bone regeneration after debridement of posttraumatic osteomyelitis is accompanied by altered cytokine levels, elevated B cell activity, and increased osteoclast activity.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society

Wagner JM,Jaurich H,Wallner C,Abraham S,Becerikli M,Dadras M,Harati K,Duhan V,Khairnar V,Lehnhardt M,Behr B

Published figure using CD19 monoclonal antibody (Product # 61-0193-82) in Flow Cytometry

Wed Nov 01 00:00:00 EDT 2017

Intravenously usable fully serotype 3 oncolytic adenovirus coding for CD40L as an enabler of dendritic cell therapy.

Oncoimmunology

Zafar S,Parviainen S,Siurala M,Hemminki O,Havunen R,Tähtinen S,Bramante S,Vassilev L,Wang H,Lieber A,Hemmi S,de Gruijl T,Kanerva A,Hemminki A

Published figure using CD19 monoclonal antibody (Product # 61-0193-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Extrafollicular CD4⁺ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

Nature communications

Deng R,Hurtz C,Song Q,Yue C,Xiao G,Yu H,Wu X,Muschen M,Forman S,Martin PJ,Zeng D

Published figure using CD19 monoclonal antibody (Product # 61-0193-82) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

Extrafollicular CD4⁺ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

Nature communications

Deng R,Hurtz C,Song Q,Yue C,Xiao G,Yu H,Wu X,Muschen M,Forman S,Martin PJ,Zeng D

Published figure using CD19 monoclonal antibody (Product # 61-0193-82) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

IL35-Producing B Cells Promote the Development of Pancreatic Neoplasia.

Cancer discovery

Pylayeva-Gupta Y,Das S,Handler JS,Hajdu CH,Coffre M,Koralov SB,Bar-Sagi D

Published figure using CD19 monoclonal antibody (Product # 61-0193-82) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2016

Medial HOXA genes demarcate haematopoietic stem cell fate during human development.

Nature cell biology

Dou DR,Calvanese V,Sierra MI,Nguyen AT,Minasian A,Saarikoski P,Sasidharan R,Ramirez CM,Zack JA,Crooks GM,Galic Z,Mikkola HK

Published figure using CD19 monoclonal antibody (Product # 61-0193-82) in Flow Cytometry

Wed Jun 01 00:00:00 EDT 2016

CD8⁺ T cells drive autoimmune hematopoietic stem cell dysfunction and bone marrow failure.

Journal of autoimmunity

Gravano DM,Al-Kuhlani M,Davini D,Sanders PD,Manilay JO,Hoyer KK

61-0193 was used in Flow cytometry/Cell sorting to investigate whether CD8+ T cells play a non-redundant role in driving bone marrow failure.

Thu Dec 01 00:00:00 EST 2016

Antigen Targeting to Human HLA Class II Molecules Increases Efficacy of DNA Vaccination.

Journal of immunology (Baltimore, Md. : 1950)

Grodeland G,Fredriksen AB,Løset GÅ,Vikse E,Fugger L,Bogen B

Published figure using CD19 monoclonal antibody (Product # 61-0193-82) in Flow Cytometry

Tue Nov 01 00:00:00 EDT 2016

PLEKHA7 defines an apical junctional complex with cytoskeletal associations and miRNA-mediated growth implications.

Cell cycle (Georgetown, Tex.)

Kourtidis A,Anastasiadis PZ

61-0193 was used in Immunocytochemistry-immunoflourescence to further investigate the function and interacting partners of PLEKHA7 at the zonula adherens.

Tue Dec 13 00:00:00 EST 2016

CD19 CAR immune pressure induces B-precursor acute lymphoblastic leukaemia lineage switch exposing inherent leukaemic plasticity.

Nature communications

Jacoby E,Nguyen SM,Fountaine TJ,Welp K,Gryder B,Qin H,Yang Y,Chien CD,Seif AE,Lei H,Song YK,Khan J,Lee DW,Mackall CL,Gardner RA,Jensen MC,Shern JF,Fry TJ

Published figure using CD19 monoclonal antibody (Product # 61-0193-82) in Flow Cytometry

Wed Jul 27 00:00:00 EDT 2016

Novel CXCL13 transgenic mouse: inflammation drives pathogenic effect of CXCL13 in experimental myasthenia gravis.

Oncotarget

Weiss JM,Robinet M,Aricha R,Cufi P,Villeret B,Lantner F,Shachar I,Fuchs S,Souroujon MC,Berrih-Aknin S,Le Panse R

Published figure using CD19 monoclonal antibody (Product # 61-0193-82) in Flow Cytometry

Tue Feb 16 00:00:00 EST 2016

Protein Kinase D2 Protects against Acute Colitis Induced by Dextran Sulfate Sodium in Mice.

Scientific reports

Xiong J,Zhou MF,Wang YD,Chen LP,Xu WF,Wang YD,Deng F,Liu SD

Published figure using CD19 monoclonal antibody (Product # 61-0193-82) in Flow Cytometry

Fri Sep 23 00:00:00 EDT 2016

Viral Infection of the Central Nervous System Exacerbates Interleukin-10 Receptor Deficiency-Mediated Colitis in SJL Mice.

PloS one

Uhde AK,Herder V,Akram Khan M,Ciurkiewicz M,Schaudien D,Teich R,Floess S,Baumgärtner W,Huehn J,Beineke A

61019382 was used in flow cytometry to study the effect of IL-10 signaling in infectious neurological diseases

Fri Aug 25 00:00:00 EDT 2017

Cis-regulatory mechanisms governing stem and progenitor cell transitions.

Science advances

Johnson KD,Kong G,Gao X,Chang YI,Hewitt KJ,Sanalkumar R,Prathibha R,Ranheim EA,Dewey CN,Zhang J,Bresnick EH

Published figure using CD19 monoclonal antibody (Product # 61-0193-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

Role of caveolin-3 in lymphocyte activation.

Life sciences

Tran C,Stary CM,Schilling JM,Bentley B,Patel HH,Roth DM

Published figure using CD19 monoclonal antibody (Product # 61-0193-82) in Flow Cytometry

Thu Jan 15 00:00:00 EST 2015

B cell Rab7 mediates induction of activation-induced cytidine deaminase expression and class-switching in T-dependent and T-independent antibody responses.

Journal of immunology (Baltimore, Md. : 1950)

Pone EJ,Lam T,Lou Z,Wang R,Chen Y,Liu D,Edinger AL,Xu Z,Casali P

Published figure using CD19 monoclonal antibody (Product # 61-0193-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2015

Mouse bone marrow-derived mesenchymal stem cells inhibit leukemia/lymphoma cell proliferation in vitro and in a mouse model of allogeneic bone marrow transplant.

International journal of molecular medicine

Song N,Gao L,Qiu H,Huang C,Cheng H,Zhou H,Lv S,Chen L,Wang J

Published figure using CD19 monoclonal antibody (Product # 61-0193-82) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2015

dNP2 is a blood-brain barrier-permeable peptide enabling ctCTLA-4 protein delivery to ameliorate experimental autoimmune encephalomyelitis.

Nature communications

Lim S,Kim WJ,Kim YH,Lee S,Koo JH,Lee JA,Yoon H,Kim DH,Park HJ,Kim HM,Lee HG,Yun Kim J,Lee JU,Hun Shin J,Kyun Kim L,Doh J,Kim H,Lee SK,Bothwell ALM,Suh M,Choi JM

Published figure using CD19 monoclonal antibody (Product # 61-0193-82) in Flow Cytometry

Tue Sep 15 00:00:00 EDT 2015

A novel method for autophagy detection in primary cells: impaired levels of macroautophagy in immunosenescent T cells.

Autophagy

Phadwal K,Alegre-Abarrategui J,Watson AS,Pike L,Anbalagan S,Hammond EM,Wade-Martins R,McMichael A,Klenerman P,Simon AK

Published figure using CD19 monoclonal antibody (Product # 61-0193-82) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2012

Critical role of B cell lymphoma 10 in BAFF-regulated NF-κB activation and survival of anergic B cells.

Journal of immunology (Baltimore, Md. : 1950)

Yu M,Chen Y,He Y,Podd A,Fu G,Wright JA,Kleiman E,Khan WN,Wen R,Wang D

Published figure using CD19 monoclonal antibody (Product # 61-0193-82) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2012

Bioinformatics

Protein Aliases: B-lymphocyte antigen CD19; CD19; Differentiation antigen CD19; Leu-12

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Gene Aliases: AW495831; Cd19

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UniProt ID: (Mouse) P25918

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Entrez Gene ID: (Mouse) 12478

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Process(es)

chromosome breakage B cell receptor signaling pathway positive regulation of release of sequestered calcium ion into cytosol

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CD19 Monoclonal Antibody (eBio1D3 (1D3)), PE-eFluor™ 610, eBioscience™

CD19 Antibody (61-0193-82) in Flow

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61-0193-82

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