Invitrogen

CD3 Monoclonal Antibody (17A2), Alexa Fluor™ 488, eBioscience™

FIGURE: 1 / 82

CD3 Antibody (53-0032-82) in Flow

C57BL/6 mouse splenocytes were stained with CD45R (B220) Monoclonal Antibody, PerCP-eFluor 710 (Product # 46-0452-82) and 0.25 µg of Rat IgG2b kappa Isotype Control, Alexa Fluor 488 (Product # 53-4031-80) (left) or 0.25 µg of CD3 Monoclonal Antibody, Alexa Fluor 488 (right). Cells in the lymphocyte gate were used for analysis.

CD3 Antibody in Immunohistochemistry (IHC)

CD3 Antibody in Immunocytochemistry (ICC/IF)

CD3 Antibody in Immunocytochemistry, Immunohistochemistry (Paraffin) (ICC/IF, IHC (P))

CD3 Antibody in Immunohistochemistry (Frozen) (IHC (F))

CD3 Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)

CD3 Antibody in Functional Assay (Functional)

Product Image Gallery

Product Details

53-0032-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 11 publications 11 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

Flow Cytometry (Flow)

0.5 µg/test

View 55 publications 55 publications

Functional Assay (Functional)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

17A2

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2848414

Product Specific Information

Description: The 17A2 monoclonal antibody reacts with the mouse CD3 complex. CD3 subunits gamma, delta and epsilon are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 17A2 to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation.

Applications Reported: This 17A2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 17A2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser

Target Information

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Scientific reports

Ochi T,Feng Y,Kitamoto S,Nagao-Kitamoto H,Kuffa P,Atarashi K,Honda K,Teitelbaum DH,Kamada N

Published figure using CD3 monoclonal antibody (Product # 53-0032-82) in Flow Cytometry

Wed Jun 15 00:00:00 EDT 2016

High salt reduces the activation of IL-4- and IL-13-stimulated macrophages.

The Journal of clinical investigation

Binger KJ,Gebhardt M,Heinig M,Rintisch C,Schroeder A,Neuhofer W,Hilgers K,Manzel A,Schwartz C,Kleinewietfeld M,Voelkl J,Schatz V,Linker RA,Lang F,Voehringer D,Wright MD,Hubner N,Dechend R,Jantsch J,Titze J,Müller DN

Published figure using CD3 monoclonal antibody (Product # 53-0032-82) in Flow Cytometry

Mon Nov 02 00:00:00 EST 2015

IKKβ acts as a tumor suppressor in cancer-associated fibroblasts during intestinal tumorigenesis.

The Journal of experimental medicine

Pallangyo CK,Ziegler PK,Greten FR

Published figure using CD3 monoclonal antibody (Product # 53-0032-82) in Flow Cytometry

Mon Dec 14 00:00:00 EST 2015

Adjuvants may reduce in vivo transfection levels for DNA vaccination in mice leading to reduced antigen-specific CD8+ T cell responses.

Human vaccines & immunotherapeutics

Rose AH,Hoffmann FW,Hara JH,Urschitz J,Moisyadi S,Hoffmann PR,Bertino P

Published figure using CD3 monoclonal antibody (Product # 53-0032-82) in Flow Cytometry

Mon May 16 00:00:00 EDT 2016

IL-33 Enhances Humoral Immunity Against Chronic HBV Infection Through Activating CD4(+)CXCR5(+) TFH Cells.

Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research

Zhao PW,Shi X,Li C,Ayana DA,Niu JQ,Feng JY,Wang J,Jiang YF

Published figure using CD3 monoclonal antibody (Product # 53-0032-82) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2015

Regulation of NKT cell-mediated immune responses to tumours and liver inflammation by mitochondrial PGAM5-Drp1 signalling.

Nature communications

Kang YJ,Bang BR,Han KH,Hong L,Shim EJ,Ma J,Lerner RA,Otsuka M

Published figure using CD3 monoclonal antibody (Product # 53-0032-82) in Flow Cytometry

Fri Sep 18 00:00:00 EDT 2015

Antibiotics in neonatal life increase murine susceptibility to experimental psoriasis.

Nature communications

Zanvit P,Konkel JE,Jiao X,Kasagi S,Zhang D,Wu R,Chia C,Ajami NJ,Smith DP,Petrosino JF,Abbatiello B,Nakatsukasa H,Chen Q,Belkaid Y,Chen ZJ,Chen W

Published figure using CD3 monoclonal antibody (Product # 53-0032-82) in Flow Cytometry

Tue Sep 29 00:00:00 EDT 2015

A chitin-like component on sclerotic cells of Fonsecaea pedrosoi inhibits Dectin-1-mediated murine Th17 development by masking β-glucans.

PloS one

Dong B,Li D,Li R,Chen SC,Liu W,Liu W,Chen L,Chen Y,Zhang X,Tong Z,Xia Y,Xia P,Wang Y,Duan Y

Published figure using CD3 monoclonal antibody (Product # 53-0032-82) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2015

Leukocyte population dynamics and detection of IL-9 as a major cytokine at the mouse fetal-maternal interface.

PloS one

Habbeddine M,Verbeke P,Karaz S,Bobé P,Kanellopoulos-Langevin C

Published figure using CD3 monoclonal antibody (Product # 53-0032-82) in Flow Cytometry

Wed Jun 10 00:00:00 EDT 2015

Interferon gamma causes olfactory dysfunction without concomitant neuroepithelial damage.

International forum of allergy & rhinology

Pozharskaya T,Lane AP

Published figure using CD3 monoclonal antibody (Product # 53-0032-82) in Immunohistochemistry

Fri Nov 01 00:00:00 EDT 2013

Increased incidence of endometrioid tumors caused by aberrations in E-cadherin promoter of mismatch repair-deficient mice.

Carcinogenesis

Kovtun IV,Harris KJ,Jatoi A,Jevremovic D

Published figure using CD3 monoclonal antibody (Product # 53-0032-82) in Immunohistochemistry

Fri Jul 01 00:00:00 EDT 2011

Auf1/Hnrnpd-deficient mice develop pruritic inflammatory skin disease.

The Journal of investigative dermatology

Sadri N,Schneider RJ

Published figure using CD3 monoclonal antibody (Product # 53-0032-82) in Flow Cytometry

Sun Mar 01 00:00:00 EST 2009

Bioinformatics

Protein Aliases: CD247; CD247 antigen; CD3 antigen, zeta polypeptide; CD3 TCR complex; CD3-zeta/eta; CD3d; CD3e; CD3g; FLJ18683; Leu-4; T cell antigen receptor complex epsilon subunit of T3; T-cell receptor T3 delta chain; T-cell receptor T3 eta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex

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Gene Aliases: 4930549J05Rik; A430104F18Rik; AI504783; AW552088; Cd247; CD3; Cd3-eta; Cd3-zeta; Cd3d; Cd3e; CD3epsilon; Cd3g; Cd3h; Cd3z; Cd3zeta; Ctg-3; Ctg3; T3d; T3e; T3g; T3z; Tcrk; Tcrz

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UniProt ID: (Mouse) P24161, (Mouse) P04235, (Mouse) P22646, (Mouse) P11942

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Entrez Gene ID: (Mouse) 12503, (Mouse) 12500, (Mouse) 12501, (Mouse) 12502

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Function(s)

transmembrane signaling receptor activity protein binding identical protein binding protein heterodimerization activity signal transducer activity SH3 domain binding defense/immunity protein immunoglobulin receptor superfamily

Process(es)

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CD3 Monoclonal Antibody (17A2), Alexa Fluor™ 488, eBioscience™

CD3 Antibody (53-0032-82) in Flow

Product Details

53-0032-82

Western Blot (WB)

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