Invitrogen

CD3 Monoclonal Antibody (17A2), Super Bright™ 780, eBioscience™

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CD3 Antibody (78-0032-82) in Flow

Swiss Webster mouse splenocytes were stained with CD45R (B220) Monoclonal Antibody, PE (Product # 12-0452-82) and 0.5 µg of Rat IgG2b kappa Isotype Control, Super Bright 780 (Product # 78-4031-82) (left) or 0.5 µg of CD3 Monoclonal Antibody, Super Bright 780 (right). Cells in the lymphocyte gate were used for analysis.

CD3 Antibody in Immunohistochemistry (IHC)

CD3 Antibody in Immunocytochemistry (ICC/IF)

CD3 Antibody in Immunocytochemistry, Immunohistochemistry (Paraffin) (ICC/IF, IHC (P))

CD3 Antibody in Immunohistochemistry (Frozen) (IHC (F))

Product Image Gallery

Product Details

78-0032-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

1.0 µg/test

View 31 publications 31 publications

Product Specifications

Species Reactivity

Mouse

Published species

Not Applicable

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), Super Bright™ 780, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

17A2

Conjugate

Super Bright™ 780 Super Bright™ 780 Super Bright™ 780

Excitation/Emission Max

413/780 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2762806

Product Specific Information

Description: The 17A2 monoclonal antibody reacts with the mouse CD3 complex. CD3 subunits gamma, delta and epsilon are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 17A2 to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation.

Applications Reported: This 17A2 antibody has been reported for use in flow cytometric analysis and functional activity (in vitro simulation).

Applications Tested: This 17A2 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This may be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Super Bright 780 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 780 nm. We recommend using a 780/60 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

In some experiments, we have observed that compensation values for Super Bright 780-conjugated antibodies are higher in the violet 450/50 channel when using UltraComp eBeads microspheres (Product # 01-2222-42) as compared to single-color stained cells. In such circumstances, we would recommend setting compensation with cells. We have also observed this in some experiments using AbC Total Antibody Compensation beads (Product # A10497).

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 405 nm; Emission: 780 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

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Loss of phosphatidylinositol-4-phosphate 5-kinase type-1 gamma (Pip5k1c) in mesenchymal stem cells leads to osteopenia by impairing bone remodeling.

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Choroid plexus-selective inactivation of adenosine A_2A receptors protects against T cell infiltration and experimental autoimmune encephalomyelitis.

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Acta pharmaceutica Sinica. B

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Early inflammation precedes cardiac fibrosis and heart failure in desmoglein 2 murine model of arrhythmogenic cardiomyopathy.

Cell and tissue research

Ng KE,Delaney PJ,Thenet D,Murtough S,Webb CM,Zaman N,Tsisanova E,Mastroianni G,Walker SLM,Westaby JD,Pennington DJ,Pink R,Kelsell DP,Tinker A

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Oncology letters

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Frontiers in immunology

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Alphavirus-Driven Interferon Gamma (IFNg) Expression Inhibits Tumor Growth in Orthotopic 4T1 Breast Cancer Model.

Vaccines

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Functional inhibition of lactate dehydrogenase suppresses pancreatic adenocarcinoma progression.

Clinical and translational medicine

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Clinical and translational medicine

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Expression of CCRL2 Inhibits Tumor Growth by Concentrating Chemerin and Inhibiting Neoangiogenesis.

Cancers

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Tue Oct 05 00:00:00 EDT 2021

Small Molecule Potentiator of Adjuvant Activity Enhancing Survival to Influenza Viral Challenge.

Frontiers in immunology

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Type 1 Cytotoxic T Cells Increase in Placenta after Intrauterine Inflammation.

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Cell reports methods

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Frontiers in immunology

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The Journal of experimental medicine

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Frontiers in immunology

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Aging

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Nature communications

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Mitophagy in Intestinal Epithelial Cells Triggers Adaptive Immunity during Tumorigenesis.

Cell

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Published figure using CD3 monoclonal antibody (Product # 78-0032-82) in Immunohistochemistry

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Mitophagy in Intestinal Epithelial Cells Triggers Adaptive Immunity during Tumorigenesis.

Cell

Published figure using CD3 monoclonal antibody (Product # 78-0032-82) in Immunohistochemistry

Thu Jun 28 00:00:00 EDT 2018

Impact of hyperglycemia on autoimmune pancreatitis and regulatory T-cells.

World journal of gastroenterology

Müller-Graff FT,Fitzner B,Jaster R,Vollmar B,Zechner D

Published figure using CD3 monoclonal antibody (Product # 78-0032-82) in Flow Cytometry

Sat Jul 28 00:00:00 EDT 2018

Microglia prevent peripheral immune cell invasion and promote an anti-inflammatory environment in the brain of APP-PS1 transgenic mice.

Journal of neuroinflammation

Unger MS,Schernthaner P,Marschallinger J,Mrowetz H,Aigner L

Published figure using CD3 monoclonal antibody (Product # 78-0032-82) in Flow Cytometry

Fri Sep 21 00:00:00 EDT 2018

CD8(+) T-cell Immune Evasion Enables Oncolytic Virus Immunotherapy.

EBioMedicine

Pourchet A,Fuhrmann SR,Pilones KA,Demaria S,Frey AB,Mulvey M,Mohr I

Published figure using CD3 monoclonal antibody (Product # 78-0032-82) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2016

Regulation of NKT cell-mediated immune responses to tumours and liver inflammation by mitochondrial PGAM5-Drp1 signalling.

Nature communications

Kang YJ,Bang BR,Han KH,Hong L,Shim EJ,Ma J,Lerner RA,Otsuka M

Published figure using CD3 monoclonal antibody (Product # 78-0032-82) in Flow Cytometry

Fri Sep 18 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: CD247; CD247 antigen; CD3 antigen, zeta polypeptide; CD3 TCR complex; CD3-zeta/eta; CD3d; CD3e; CD3g; FLJ18683; Leu-4; T cell antigen receptor complex epsilon subunit of T3; T-cell receptor T3 delta chain; T-cell receptor T3 eta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex

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Gene Aliases: 4930549J05Rik; A430104F18Rik; AI504783; AW552088; Cd247; CD3; Cd3-eta; Cd3-zeta; Cd3d; Cd3e; CD3epsilon; Cd3g; Cd3h; Cd3z; Cd3zeta; Ctg-3; Ctg3; T3d; T3e; T3g; T3z; Tcrk; Tcrz

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UniProt ID: (Mouse) P24161, (Mouse) P04235, (Mouse) P22646, (Mouse) P11942

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Entrez Gene ID: (Mouse) 12503, (Mouse) 12500, (Mouse) 12501, (Mouse) 12502

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Function(s)

transmembrane signaling receptor activity protein binding identical protein binding protein heterodimerization activity signal transducer activity SH3 domain binding defense/immunity protein immunoglobulin receptor superfamily

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD3 Monoclonal Antibody (17A2), Super Bright™ 780, eBioscience™

CD3 Antibody (78-0032-82) in Flow

Product Details

78-0032-82

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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