Invitrogen

CD115 (c-fms) Monoclonal Antibody (AFS98), Alexa Fluor™ 488, eBioscience™

CD115 (c-fms) Antibody in Flow Cytometry (Flow)

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CD115 (c-fms) Antibody (53-1152-82) in Flow

Staining of thioglycolate-induced peritoneal exudeate cells (PECs) with 0.25 µg of Rat IgG2a kappa Isotype Control Alexa Fluor® 488 (Product # 53-4321-80) (blue histogram) or 0.25 µg of Anti-Mouse CD115 (c-fms) Alexa Fluor® 488 (purple histogram). Total viable cells were used for analysis.

CD115 (c-fms) Antibody in Immunocytochemistry (ICC/IF)

CD115 (c-fms) Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

53-1152-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test

View 32 publications 32 publications

Product Specifications

Species Reactivity

Mouse

Published species

Fish, Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

AFS98

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2016696

Product Specific Information

Description: The AFS98 monoclonal antibody reacts with the mouse CD115 molecule, a receptor for macrophage colony stimulating factor (M-CSF) or colony stimulating factor-1 (CSF-1). CD115 is expressed by monocyte, macrophage, osteoclast, and some epithelial cells. It is a 150 kDa c-fms gene product and belongs to immunoglobulin family. CSF-1 signaling through CSF-1R regulates the proliferation and differentiation of cells in the monocytic lineage.

Applications Reported: This AFS98 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This AFS98 antibody has been tested by flow cytometric analysis of mouse peritoneal elicited cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10e5 to 10e8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CSF1R (FMS) is involved in the production, differentiation, and function of macrophages, and is implicated in promyelocytic leukemias. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. CSF1R is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in the gene encoding CSF1R have been associated with a predisposition to myeloid malignancy.Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and plays an important role in innate immunity and in inflammatory processes. The first intron of the CSF1R gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction. Mutations in the CSF1R gene have been associated with a predisposition to myeloid malignancy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Pyruvate dehydrogenase kinase supports macrophage NLRP3 inflammasome activation during acute inflammation.

Cell reports

Meyers AK,Wang Z,Han W,Zhao Q,Zabalawi M,Duan L,Liu J,Zhang Q,Manne RK,Lorenzo F,Quinn MA,Song Q,Fan D,Lin HK,Furdui CM,Locasale JW,McCall CE,Zhu X

Published figure using CD115 (c-fms) monoclonal antibody (Product # 53-1152-82) in Flow Cytometry

Tue Jan 31 00:00:00 EST 2023

Platelets exacerbate cardiovascular inflammation in a murine model of Kawasaki disease vasculitis.

JCI insight

Kocatürk B,Lee Y,Nosaka N,Abe M,Martinon D,Lane ME,Moreira D,Chen S,Fishbein MC,Porritt RA,Franklin BS,Noval Rivas M,Arditi M

Published figure using CD115 (c-fms) monoclonal antibody (Product # 53-1152-82) in Flow Cytometry

Mon Jul 24 00:00:00 EDT 2023

Pexidartinib synergize PD-1 antibody through inhibiting treg infiltration by reducing TAM-derived CCL22 in lung adenocarcinoma.

Frontiers in pharmacology

Zhang W,Jiang X,Zou Y,Yuan L,Wang X

Published figure using CD115 (c-fms) monoclonal antibody (Product # 53-1152-82) in Flow Cytometry

Tue Mar 28 00:00:00 EDT 2023

MARCH3 negatively regulates IL-3-triggered inflammatory response by mediating K48-linked polyubiquitination and degradation of IL-3Rα.

Signal transduction and targeted therapy

Feng L,Li C,Zeng LW,Gao D,Sun YH,Zhong L,Lin H,Shu HB,Li S

Published figure using CD115 (c-fms) monoclonal antibody (Product # 53-1152-82) in Flow Cytometry

Mon Jan 24 00:00:00 EST 2022

Loss of phosphatidylinositol-4-phosphate 5-kinase type-1 gamma (Pip5k1c) in mesenchymal stem cells leads to osteopenia by impairing bone remodeling.

The Journal of biological chemistry

Yan Q,Gao H,Yao Q,Ling K,Xiao G

Published figure using CD115 (c-fms) monoclonal antibody (Product # 53-1152-82) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2022

Mapping the origin and fate of myeloid cells in distinct compartments of the eye by single-cell profiling.

The EMBO journal

Wieghofer P,Hagemeyer N,Sankowski R,Schlecht A,Staszewski O,Amann L,Gruber M,Koch J,Hausmann A,Zhang P,Boneva S,Masuda T,Hilgendorf I,Goldmann T,Böttcher C,Priller J,Rossi FM,Lange C,Prinz M

Published figure using CD115 (c-fms) monoclonal antibody (Product # 53-1152-82) in Flow Cytometry

Mon Mar 15 00:00:00 EDT 2021

Low-dose 2-deoxy glucose stabilises tolerogenic dendritic cells and generates potent in vivo immunosuppressive effects.

Cellular and molecular life sciences : CMLS

Christofi M,Le Sommer S,Mölzer C,Klaska IP,Kuffova L,Forrester JV

Published figure using CD115 (c-fms) monoclonal antibody (Product # 53-1152-82) in Flow Cytometry

Mon Mar 01 00:00:00 EST 2021

HOXA11 plays critical roles in disease progression and response to cytarabine in AML.

Oncology reports

Fu JF,Shih LY,Yen TH

Published figure using CD115 (c-fms) monoclonal antibody (Product # 53-1152-82) in Flow Cytometry

Thu Jul 01 00:00:00 EDT 2021

Inhibition of TLR4 signaling protects mice from sensory and motor dysfunction in an animal model of autoimmune peripheral neuropathy.

Journal of neuroinflammation

Oladiran O,Shi XQ,Yang M,Fournier S,Zhang J

53-1152 was used in Flow cytometry/Cell sorting to identify the critical contribution of TLR4-mediated macrophage activation in disease course and provided strong evidence to support TLR4 as a useful drug target for treating inflammatory autoimmune neuropathy.

Mon Mar 22 00:00:00 EDT 2021

Inhibition of TLR4 signaling protects mice from sensory and motor dysfunction in an animal model of autoimmune peripheral neuropathy.

Journal of neuroinflammation

Oladiran O,Shi XQ,Yang M,Fournier S,Zhang J

Mon Mar 22 00:00:00 EDT 2021

CX3CR1 But Not CCR2 Expression Is Required for the Development of Autoimmune Peripheral Neuropathy in Mice.

Frontiers in immunology

Oladiran O,Shi XQ,Fournier S,Zhang J

Published figure using CD115 (c-fms) monoclonal antibody (Product # 53-1152-82) in Flow Cytometry

Tue Dec 21 00:00:00 EST 2021

Inflammation rapidly recruits mammalian GMP and MDP from bone marrow into regional lymphatics.

eLife

Serrano-Lopez J,Hegde S,Kumar S,Serrano J,Fang J,Wellendorf AM,Roche PA,Rangel Y,Carrington LJ,Geiger H,Grimes HL,Luther S,Maillard I,Sanchez-Garcia J,Starczynowski DT,Cancelas JA

Published figure using CD115 (c-fms) monoclonal antibody (Product # 53-1152-82) in Flow Cytometry

Thu Apr 08 00:00:00 EDT 2021

Hepatocyte-specific glucose-6-phosphatase deficiency disturbs platelet aggregation and decreases blood monocytes upon fasting-induced hypoglycemia.

Molecular metabolism

La Rose AM,Bazioti V,Hoogerland JA,Svendsen AF,Groenen AG,van Faassen M,Rutten MGS,Kloosterhuis NJ,Dethmers-Ausema B,Nijland JH,Mithieux G,Rajas F,Kuipers F,Lukens MV,Soehnlein O,Oosterveer MH,Westerterp M

Published figure using CD115 (c-fms) monoclonal antibody (Product # 53-1152-82) in Flow Cytometry

Mon Nov 01 00:00:00 EDT 2021

Mesenchymal stem cells attenuate liver fibrosis by targeting Ly6C^hi/lo macrophages through activating the cytokine-paracrine and apoptotic pathways.

Cell death discovery

Li YH,Shen S,Shao T,Jin MT,Fan DD,Lin AF,Xiang LX,Shao JZ

53-1152-82 was used in Flow Cytometry to provide insights into the functional role of bone marrow-derived sesenchymal stem cell in alleviating liver fibrosis.

Mon Sep 13 00:00:00 EDT 2021

Radiation causes tissue damage by dysregulating inflammasome-gasdermin D signaling in both host and transplanted cells.

PLoS biology

Xiao J,Wang C,Yao JC,Alippe Y,Yang T,Kress D,Sun K,Kostecki KL,Monahan JB,Veis DJ,Abu-Amer Y,Link DC,Mbalaviele G

Published figure using CD115 (c-fms) monoclonal antibody (Product # 53-1152-82) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2020

Temporospatial distribution and transcriptional profile of retinal microglia in the oxygen-induced retinopathy mouse model.

Glia

Boeck M,Thien A,Wolf J,Hagemeyer N,Laich Y,Yusuf D,Backofen R,Zhang P,Boneva S,Stahl A,Hilgendorf I,Agostini H,Prinz M,Wieghofer P,Schlunck G,Schlecht A,Lange C

Published figure using CD115 (c-fms) monoclonal antibody (Product # 53-1152-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2020

Androgen Receptor Signaling Positively Regulates Monocytic Development.

Frontiers in immunology

Consiglio CR,Gollnick SO

Published figure using CD115 (c-fms) monoclonal antibody (Product # 53-1152-82) in Flow Cytometry

Thu Apr 29 00:00:00 EDT 2021

Inflammasome Activation Triggers Blood Clotting and Host Death through Pyroptosis.

Immunity

Wu C,Lu W,Zhang Y,Zhang G,Shi X,Hisada Y,Grover SP,Zhang X,Li L,Xiang B,Shi J,Li XA,Daugherty A,Smyth SS,Kirchhofer D,Shiroishi T,Shao F,Mackman N,Wei Y,Li Z

53-1152-82 was used in Flow Cytometry to identify inflammasome activation as a trigger for blood clotting through pyroptosis.

Tue Jun 18 00:00:00 EDT 2019

Concomitant type I IFN and M-CSF signaling reprograms monocyte differentiation and drives pro-tumoral arginase production.

EBioMedicine

Tong Y,Zhou L,Yang L,Guo P,Cao Y,Qin FX,Liu J

Published figure using CD115 (c-fms) monoclonal antibody (Product # 53-1152-82) in Flow Cytometry

Tue Jan 01 00:00:00 EST 2019

Bone loss in C57BL/6J-OlaHsd mice, a substrain of C57BL/6J carrying mutated alpha-synuclein and multimerin-1 genes.

Journal of cellular physiology

Liron T,Raphael B,Hiram-Bab S,Bab IA,Gabet Y

Published figure using CD115 (c-fms) monoclonal antibody (Product # 53-1152-82) in Flow Cytometry

Mon Jan 01 00:00:00 EST 2018

Captopril mitigates splenomegaly and myelofibrosis in the Gata1^low murine model of myelofibrosis.

Journal of cellular and molecular medicine

Corey SJ,Jha J,McCart EA,Rittase WB,George J,Mattapallil JJ,Mehta H,Ognoon M,Bylicky MA,Summers TA,Day RM

Published figure using CD115 (c-fms) monoclonal antibody (Product # 53-1152-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

Neutrophil and Macrophage Cell Surface Colony-Stimulating Factor 1 Shed by ADAM17 Drives Mouse Macrophage Proliferation in Acute and Chronic Inflammation.

Molecular and cellular biology

Tang J,Frey JM,Wilson CL,Moncada-Pazos A,Levet C,Freeman M,Rosenfeld ME,Stanley ER,Raines EW,Bornfeldt KE

Published figure using CD115 (c-fms) monoclonal antibody (Product # 53-1152-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

Advanced Glycation End Products Enhance Murine Monocyte Proliferation in Bone Marrow and Prime Them into an Inflammatory Phenotype through MAPK Signaling.

Journal of diabetes research

Jin X,Liu L,Zhang Y,Xiang Y,Yin G,Lu Y,Shi L,Dong J,Shen C

Published figure using CD115 (c-fms) monoclonal antibody (Product # 53-1152-82) in Flow Cytometry

Wed Oct 10 00:00:00 EDT 2018

Erythropoietin enhances Kupffer cell number and activity in the challenged liver.

Scientific reports

Gilboa D,Haim-Ohana Y,Deshet-Unger N,Ben-Califa N,Hiram-Bab S,Reuveni D,Zigmond E,Gassmann M,Gabet Y,Varol C,Neumann D

Published figure using CD115 (c-fms) monoclonal antibody (Product # 53-1152-82) in Flow Cytometry

Mon Sep 04 00:00:00 EDT 2017

Enforced Expression of Hoxa3 Inhibits Classical and Promotes Alternative Activation of Macrophages In Vitro and In Vivo.

Journal of immunology (Baltimore, Md. : 1950)

Al Sadoun H,Burgess M,Hentges KE,Mace KA

Published figure using CD115 (c-fms) monoclonal antibody (Product # 53-1152-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

PER1 prevents excessive innate immune response during endotoxin-induced liver injury through regulation of macrophage recruitment in mice.

Cell death & disease

Wang T,Wang Z,Yang P,Xia L,Zhou M,Wang S,Du J,Zhang J

Published figure using CD115 (c-fms) monoclonal antibody (Product # 53-1152-82) in Flow Cytometry

Thu Apr 07 00:00:00 EDT 2016

Spatiotemporal expression dynamics of selectins govern the sequential extravasation of neutrophils and monocytes in the acute inflammatory response.

Arteriosclerosis, thrombosis, and vascular biology

Zuchtriegel G,Uhl B,Hessenauer ME,Kurz AR,Rehberg M,Lauber K,Krombach F,Reichel CA

53-1152 was used in Flow cytometry/Cell sorting to provide novel insights into the mechanisms initiating the sequential infiltration of the perivascular tissue by neutrophils and monocytes in the acute inflammatory response.

Wed Apr 01 00:00:00 EDT 2015

Cellular changes in blood indicate severe respiratory disease during influenza infections in mice.

PloS one

Dengler L,Kühn N,Shin DL,Hatesuer B,Schughart K,Wilk E

53-1152 was used in Flow cytometry/Cell sorting to investigate the link between granulocyte to lymphocyte ratio in the blood and severe influenza disease in mice, to enable improvement of early diagnostic options of severe influenza disease.

Mon Jan 18 00:00:00 EST 2016

miR-126 and miR-126* repress recruitment of mesenchymal stem cells and inflammatory monocytes to inhibit breast cancer metastasis.

Nature cell biology

Zhang Y,Yang P,Sun T,Li D,Xu X,Rui Y,Li C,Chong M,Ibrahim T,Mercatali L,Amadori D,Lu X,Xie D,Li QJ,Wang XF

Published figure using CD115 (c-fms) monoclonal antibody (Product # 53-1152-82) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2013

Inhibition of Ca²⁺/calmodulin-dependent protein kinase kinase 2 stimulates osteoblast formation and inhibits osteoclast differentiation.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Cary RL,Waddell S,Racioppi L,Long F,Novack DV,Voor MJ,Sankar U

53-1152 was used in Flow cytometry/Cell sorting to reveal a novel function for CaMKK2 in bone remodelling and highlight the potential for therapeutic inhibition as a bone anabolic strategy.

Mon Jul 01 00:00:00 EDT 2013

Endothelial cells provide an instructive niche for the differentiation and functional polarization of M2-like macrophages.

Blood

He H,Xu J,Warren CM,Duan D,Li X,Wu L,Iruela-Arispe ML

Published figure using CD115 (c-fms) monoclonal antibody (Product # 53-1152-82) in Immunofluorescence

Thu Oct 11 00:00:00 EDT 2012

In vivo inhibition of c-MYC in myeloid cells impairs tumor-associated macrophage maturation and pro-tumoral activities.

PloS one

Pello OM,Chèvre R,Laoui D,De Juan A,Lolo F,Andrés-Manzano MJ,Serrano M,Van Ginderachter JA,Andrés V

Published figure using CD115 (c-fms) monoclonal antibody (Product # 53-1152-82) in Flow Cytometry

Mon Feb 25 00:00:00 EST 2013

MicroRNA-33 deficiency reduces the progression of atherosclerotic plaque in ApoE-/- mice.

Journal of the American Heart Association

Horie T,Baba O,Kuwabara Y,Chujo Y,Watanabe S,Kinoshita M,Horiguchi M,Nakamura T,Chonabayashi K,Hishizawa M,Hasegawa K,Kume N,Yokode M,Kita T,Kimura T,Ono K

53-1152 was used in Flow cytometry/Cell sorting to investigate the impact of the genetic loss of miR-33 in a mouse model of atherosclerosis.

Sat Dec 01 00:00:00 EST 2012

Stat3-dependent acute Rantes production in vascular smooth muscle cells modulates inflammation following arterial injury in mice.

The Journal of clinical investigation

Kovacic JC,Gupta R,Lee AC,Ma M,Fang F,Tolbert CN,Walts AD,Beltran LE,San H,Chen G,St Hilaire C,Boehm M

Published figure using CD115 (c-fms) monoclonal antibody (Product # 53-1152-82) in Immunofluorescence

Fri Jan 01 00:00:00 EST 2010

Phospholipase Cgamma2 mediates RANKL-stimulated lymph node organogenesis and osteoclastogenesis.

The Journal of biological chemistry

Chen Y,Wang X,Di L,Fu G,Chen Y,Bai L,Liu J,Feng X,McDonald JM,Michalek S,He Y,Yu M,Fu YX,Wen R,Wu H,Wang D

Published figure using CD115 (c-fms) monoclonal antibody (Product # 53-1152-82) in Flow Cytometry

Fri Oct 24 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: c-fms; CD115; CSF-1 receptor; CSF-1-R; CSFIR; EC 2.7.10.1; kinase CSFR; Macrophage colony-stimulating factor 1 receptor; OTTHUMP00000224093; Proto-oncogene c-Fms; proto-oncogene fms

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Gene Aliases: AI323359; CD115; CSF-1R; Csf1r; Csfmr; Fim-2; Fms; M-CSF-R; M-CSFR

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UniProt ID: (Mouse) P09581

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Entrez Gene ID: (Mouse) 12978

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Function(s)

nucleotide binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity macrophage colony-stimulating factor receptor activity protein binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein phosphatase binding cytokine binding protein homodimerization activity transmembrane signal receptor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD115 (c-fms) Monoclonal Antibody (AFS98), Alexa Fluor™ 488, eBioscience™

CD115 (c-fms) Antibody (53-1152-82) in Flow

Product Details

53-1152-82

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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