Invitrogen

CD117 (c-Kit) Monoclonal Antibody (ACK2), Alexa Fluor™ 700, eBioscience™

CD117 (c-Kit) Antibody in Flow Cytometry (Flow)

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CD117 (c-Kit) Antibody (56-1172-82) in Flow

C57BL/6 mouse bone marrow cells were stained with Sca-1 Monoclonal Antibody, FITC (Product # 11-5981-82) and 0.06 µg of Rat IgG2b kappa Isotype Control, Alexa Fluor 700 (Product # 56-4031-80) (left) or 0.06 µg of CD117 (c-Kit) Monoclonal Antibody, Alexa Fluor 700 (right). Cells in the Lineage (Product # 88-7772-72) low/negative gate were used for analysis.

CD117 (c-Kit) Antibody in Immunocytochemistry (ICC/IF)

CD117 (c-Kit) Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

56-1172-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 11 publications 11 publications

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Flow Cytometry (Flow)

0.06 µg/test

View 18 publications 18 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), Alexa Fluor™ 700, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

ACK2

Conjugate

Alexa Fluor™ 700 Alexa Fluor™ 700 Alexa Fluor™ 700

Excitation/Emission Max

696/719 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_657583

Product Specific Information

Description: The ACK2 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by a majority of hematopoietic progenitor cells characterized in the mouse bone marrow as a small subset of cells positive for Sca-1 and Thy1 (Thy1^lo) and negative for lineage markers. The interaction of the mouse c-kit receptor and steel factor promotes the proliferation and differentiation of hematopoietic progenitor cells. CD117 is also expressed by mast cells and plays a role in signaling and activation of these cells. ACK2 has been reported to be a blocking antibody.

Applications Reported: This ACK2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This ACK2 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT together with its ligand regulates growth and activation of a variety of hemopoietic and non-hemopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Recently, deregulation of the KIT receptor TK by the prevalent activation loop mutation D816V has served as a focal point in therapeutic strategies aimed at curbing neoplastic mast cell growth. c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells and gastrointestinal tract cajal cells. Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of various tyrosine residues including tyrosine 703 located on the cytoplasmic domain of the receptor. This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma and JAK/STAT. c-kit receptor activation is essential for hematopoiesis, stem cell maintenance and gametogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Engineered bone marrow as a clinically relevant ex vivo model for primary bone cancer research and drug screening.

Proceedings of the National Academy of Sciences of the United States of America

Griffin KH,Thorpe SW,Sebastian A,Hum NR,Coonan TP,Sagheb IS,Loots GG,Randall RL,Leach JK

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 56-1172-82) in Flow Cytometry

Tue Sep 26 00:00:00 EDT 2023

Landscape of mast cell populations across organs in mice and humans.

The Journal of experimental medicine

Tauber M,Basso L,Martin J,Bostan L,Pinto MM,Thierry GR,Houmadi R,Serhan N,Loste A,Blériot C,Kamphuis JBJ,Grujic M,Kjellén L,Pejler G,Paul C,Dong X,Galli SJ,Reber LL,Ginhoux F,Bajenoff M,Gentek R,Gaudenzio N

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 56-1172-82) in Flow Cytometry

Mon Oct 02 00:00:00 EDT 2023

Morphology of migrating telocytes and their potential role in stem cell differentiation during cartilage development in catfish (Clarias gariepinus).

Microscopy research and technique

Abd-Elhafeez HH,Rutland CS,Soliman SA

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 56-1172-82) in Immunohistochemistry

Fri Sep 01 00:00:00 EDT 2023

Two distinctive types of telocytes in gills of fish: A light, immunohistochemical and ultra-structure study.

Microscopy research and technique

Soliman SA,Sobh A,Ali LA,Abd-Elhafeez HH

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 56-1172-82) in Immunohistochemistry

Tue Nov 01 00:00:00 EDT 2022

The AML-associated K313 mutation enhances C/EBPα activity by leading to C/EBPα overexpression.

Cell death & disease

Gentle IE,Moelter I,Badr MT,Döhner K,Lübbert M,Häcker G

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 56-1172-82) in Flow Cytometry

Mon Jul 05 00:00:00 EDT 2021

The lifespan quantitative trait locus gene Securin controls hematopoietic progenitor cell function.

Haematologica

Brown A,Schuetz D,Han Y,Daria D,Nattamai KJ,Eiwen K,Sakk V,Pospiech J,Saller T,van Zant G,Wagner W,Geiger H

56-1172 was used in Flow cytometry/Cell sorting to investigate the pituitary tumour-transforming gene-1 (pttg1)/securin and it's role in regulating differential responses of hematopoietic stem and progenitor cells.

Tue Apr 27 00:00:00 EDT 2021

The lifespan quantitative trait locus gene Securin controls hematopoietic progenitor cell function.

Haematologica

Brown A,Schuetz D,Han Y,Daria D,Nattamai KJ,Eiwen K,Sakk V,Pospiech J,Saller T,van Zant G,Wagner W,Geiger H

Tue Apr 27 00:00:00 EDT 2021

Pyruvate kinase M2 in lung APCs regulates Alternaria-induced airway inflammation.

Immunobiology

Jaiswal AK,Makhija S,Stahr N,Sandey M,Suryawanshi A,Mishra A

56-1172-82 was used in Flow Cytometry to support a model in which Alternaria sensitization in airways induce a circuitry of glycolysis and PKM2 regulation that confers an acute activation of APCs in the lung, whose targeting might represent a strategy for asthma treatment.

Wed Jul 01 00:00:00 EDT 2020

Morphological and immunohistochemical phenotype of TCs in the intestinal bulb of Grass carp and their potential role in intestinal immunity.

Scientific reports

Abd-Elhafeez HH,Abou-Elhamd AS,Soliman SA

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 56-1172-82) in Immunohistochemistry

Thu Aug 20 00:00:00 EDT 2020

The lysophospholipase D enzyme Gdpd3 is required to maintain chronic myelogenous leukaemia stem cells.

Nature communications

Naka K,Ochiai R,Matsubara E,Kondo C,Yang KM,Hoshii T,Araki M,Araki K,Sotomaru Y,Sasaki K,Mitani K,Kim DW,Ooshima A,Kim SJ

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 56-1172-82) in Flow Cytometry

Thu Sep 17 00:00:00 EDT 2020

Inhibition of FGF2-Mediated Signaling in GIST-Promising Approach for Overcoming Resistance to Imatinib.

Cancers

Boichuk S,Galembikova A,Mikheeva E,Bikinieva F,Aukhadieva A,Dunaev P,Khalikov D,Petrov S,Kurtasanov R,Valeeva E,Kireev I,Dugina V,Lushnikova A,Novikova M,Kopnin P

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 56-1172-82) in Immunohistochemistry

Wed Jun 24 00:00:00 EDT 2020

Fish telocytes and their relation to rodlet cells in ruby-red-fin shark (rainbow shark) Epalzeorhynchos frenatum (Teleostei: Cyprinidae).

Scientific reports

Abd-Elhafeez HH,Abdo W,Kamal BM,Soliman SA

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 56-1172-82) in Immunohistochemistry

Tue Nov 03 00:00:00 EST 2020

Loss of FGFR3 Delays Acute Myeloid Leukemogenesis by Programming Weakly Pathogenic CD117-Positive Leukemia Stem-Like Cells.

Frontiers in pharmacology

Guo C,Ran Q,Sun C,Zhou T,Yang X,Zhang J,Pang S,Xiao Y

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 56-1172-82) in Flow Cytometry

Tue Feb 16 00:00:00 EST 2021

Id1 and Id3 Maintain Steady-State Hematopoiesis by Promoting Sinusoidal Endothelial Cell Survival and Regeneration.

Cell reports

Gadomski S,Singh SK,Singh S,Sarkar T,Klarmann KD,Berenschot M,Seaman S,Jakubison B,Gudmundsson KO,Lockett S,Keller JR

56-1172 was used in Flow cytometry/Cell sorting to investigate the effects of loss of ld genes on adult endothelial cell.

Tue Apr 28 00:00:00 EDT 2020

Hsd3b2 associated in modulating steroid hormone synthesis pathway regulates the differentiation of chicken embryonic stem cells into spermatogonial stem cells.

Journal of cellular biochemistry

Zhang C,Wang M,He N,Ahmed MF,Wang Y,Zhao R,Yu X,Jin J,Song J,Zuo Q,Zhang Y,Li B

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 56-1172-82) in Immunocytochemistry

Mon Jan 01 00:00:00 EST 2018

Electroacupuncture at ST36 Increases Bone Marrow-Derived Interstitial Cells of Cajal via the SDF-1/CXCR4 and mSCF/Kit-ETV1 Pathways in the Stomach of Diabetic Mice.

Evidence-based complementary and alternative medicine : eCAM

Zhao J,An J,Liu S

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 56-1172-82) in Immunofluorescence

Wed Mar 16 00:00:00 EDT 2022

Bioengineering functional smooth muscle with spontaneous rhythmic contraction in vitro.

Scientific reports

Kobayashi M,Khalil HA,Lei NY,Wang Q,Wang K,Wu BM,Dunn JCY

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 56-1172-82) in Immunofluorescence

Mon Sep 10 00:00:00 EDT 2018

GATA4-dependent organ-specific endothelial differentiation controls liver development and embryonic hematopoiesis.

The Journal of clinical investigation

Géraud C,Koch PS,Zierow J,Klapproth K,Busch K,Olsavszky V,Leibing T,Demory A,Ulbrich F,Diett M,Singh S,Sticht C,Breitkopf-Heinlein K,Richter K,Karppinen SM,Pihlajaniemi T,Arnold B,Rodewald HR,Augustin HG,Schledzewski K,Goerdt S

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 56-1172-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2017

Conditional genetic deletion of Ano1 in interstitial cells of Cajal impairs Ca²⁺ transients and slow waves in adult mouse small intestine.

American journal of physiology. Gastrointestinal and liver physiology

Malysz J,Gibbons SJ,Saravanaperumal SA,Du P,Eisenman ST,Cao C,Oh U,Saur D,Klein S,Ordog T,Farrugia G

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 56-1172-82) in Immunofluorescence

Wed Mar 01 00:00:00 EST 2017

Low Testosterone Alters the Activity of Mouse Prostate Stem Cells.

The Prostate

Zhou Y,Copeland B,Otto-Duessel M,He M,Markel S,Synold TW,Jones JO

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 56-1172-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

A Functional Bioluminescent Zebrafish Screen for Enhancing Hematopoietic Cell Homing.

Stem cell reports

Astuti Y,Kramer AC,Blake AL,Blazar BR,Tolar J,Taisto ME,Lund TC

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 56-1172-82) in Flow Cytometry

Tue Jan 10 00:00:00 EST 2017

Mutant U2AF1-expressing cells are sensitive to pharmacological modulation of the spliceosome.

Nature communications

Shirai CL,White BS,Tripathi M,Tapia R,Ley JN,Ndonwi M,Kim S,Shao J,Carver A,Saez B,Fulton RS,Fronick C,O'Laughlin M,Lagisetti C,Webb TR,Graubert TA,Walter MJ

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 56-1172-82) in Flow Cytometry

Mon Jan 09 00:00:00 EST 2017

Longitudinal intravital imaging of the femoral bone marrow reveals plasticity within marrow vasculature.

Nature communications

Reismann D,Stefanowski J,Günther R,Rakhymzhan A,Matthys R,Nützi R,Zehentmeier S,Schmidt-Bleek K,Petkau G,Chang HD,Naundorf S,Winter Y,Melchers F,Duda G,Hauser AE,Niesner RA

56-1172 was used in Flow cytometry/Cell sorting to develop longitudinal intravital imaging of the bone marrow to analyse cellular dynamics within the deep marrow.

Mon Dec 18 00:00:00 EST 2017

Core Circadian Clock Genes Regulate Leukemia Stem Cells in AML.

Cell

Puram RV,Kowalczyk MS,de Boer CG,Schneider RK,Miller PG,McConkey M,Tothova Z,Tejero H,Heckl D,Järås M,Chen MC,Li H,Tamayo A,Cowley GS,Rozenblatt-Rosen O,Al-Shahrour F,Regev A,Ebert BL

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 56-1172-82) in Flow Cytometry

Thu Apr 07 00:00:00 EDT 2016

Stimulus-induced pacemaker activity in interstitial cells of Cajal associated with the deep muscular plexus of the small intestine.

Neurogastroenterology and motility

Zhu YF,Wang XY,Parsons SP,Huizinga JD

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 56-1172-82) in Immunocytochemistry

Fri Jul 01 00:00:00 EDT 2016

Regulation of B-cell development and tolerance by different members of the miR-17∼92 family microRNAs.

Nature communications

Lai M,Gonzalez-Martin A,Cooper AB,Oda H,Jin HY,Shepherd J,He L,Zhu J,Nemazee D,Xiao C

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 56-1172-82) in Flow Cytometry

Tue Aug 02 00:00:00 EDT 2016

ILC3 GM-CSF production and mobilisation orchestrate acute intestinal inflammation.

eLife

Pearson C,Thornton EE,McKenzie B,Schaupp AL,Huskens N,Griseri T,West N,Tung S,Seddon BP,Uhlig HH,Powrie F

56-1172 was used in Flow cytometry/Cell sorting to investigate how a small population of innate lymphoid cells (ILCs) has large effects on immune homeostasis, showing that ILC3s produce GM-CSF to orchestrate acute intestinal inflammation.

Mon Jan 18 00:00:00 EST 2016

The origin of segmentation motor activity in the intestine.

Nature communications

Huizinga JD,Chen JH,Zhu YF,Pawelka A,McGinn RJ,Bardakjian BL,Parsons SP,Kunze WA,Wu RY,Bercik P,Khoshdel A,Chen S,Yin S,Zhang Q,Yu Y,Gao Q,Li K,Hu X,Zarate N,Collins P,Pistilli M,Ma J,Zhang R,Chen D

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 56-1172-82) in Immunofluorescence

Mon Nov 02 00:00:00 EST 2015

TRPA1-dependent pruritus in IL-13-induced chronic atopic dermatitis.

Journal of immunology (Baltimore, Md. : 1950)

Oh MH,Oh SY,Lu J,Lou H,Myers AC,Zhu Z,Zheng T

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 56-1172-82) in Immunohistochemistry

Sun Dec 01 00:00:00 EST 2013

TRPA1-dependent pruritus in IL-13-induced chronic atopic dermatitis.

Journal of immunology (Baltimore, Md. : 1950)

Oh MH,Oh SY,Lu J,Lou H,Myers AC,Zhu Z,Zheng T

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 56-1172-82) in Immunohistochemistry

Sun Dec 01 00:00:00 EST 2013

Mast cell deficiency attenuates progression of atherosclerosis and hepatic steatosis in apolipoprotein E-null mice.

American journal of physiology. Heart and circulatory physiology

Smith DD,Tan X,Raveendran VV,Tawfik O,Stechschulte DJ,Dileepan KN

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 56-1172-82) in Immunohistochemistry

Fri Jun 15 00:00:00 EDT 2012

An information theoretic, microfluidic-based single cell analysis permits identification of subpopulations among putatively homogeneous stem cells.

PloS one

Glotzbach JP,Januszyk M,Vial IN,Wong VW,Gelbard A,Kalisky T,Thangarajah H,Longaker MT,Quake SR,Chu G,Gurtner GC

56-1172 was used in Flow cytometry/Cell sorting to develop a novel method to characterize and predict transcriptional programs across individual homogeneous stem cells.

Wed Nov 02 00:00:00 EDT 2011

Homeostatic role of transforming growth factor-beta in the oral cavity and esophagus of mice and its expression by mast cells in these tissues.

The American journal of pathology

Vitsky A,Waire J,Pawliuk R,Bond A,Matthews D,Lacasse E,Hawes ML,Nelson C,Richards S,Piepenhagen PA,Garman RD,Andrews L,Thurberg BL,Lonning S,Ledbetter S,Ruzek MC

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 56-1172-82) in Immunofluorescence

Mon Jun 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: belly-spot; c-kit proto-oncogene protein; CD117; ckit; dominant spotting; Dominant white spotting; Mast/stem cell growth factor receptor Kit; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCFR; spotted sterile male; Steel Factor Receptor; Tyrosine-protein kinase Kit

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Gene Aliases: Bs; c-KIT; CD117; Fdc; Gsfsco1; Gsfsco5; Gsfsow3; Kit; SCO1; SCO5; Sl; SOW3; Ssm; Tr-kit; W

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UniProt ID: (Mouse) P05532

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Entrez Gene ID: (Mouse) 16590

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Function(s)

nucleotide binding protease binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity stem cell factor receptor activity protein binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups cytokine binding protein homodimerization activity metal ion binding transmembrane signal receptor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD117 (c-Kit) Monoclonal Antibody (ACK2), Alexa Fluor™ 700, eBioscience™

CD117 (c-Kit) Antibody (56-1172-82) in Flow

Product Details

56-1172-82

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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