Invitrogen

CD184 (CXCR4) Monoclonal Antibody (2B11), APC, eBioscience™

CD184 (CXCR4) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 14

CD184 (CXCR4) Antibody (17-9991-82) in Flow

Staining of BALB/c thymocytes with Anti-Mouse CD4 FITC (Product # 11-0041-82) and 0.25 µg of Rat IgG2b kappa Isotype Control APC (Product # 17-4031-82) (left) or 0.25 µg of Anti-Mouse CD184 (CXCR4) APC (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

17-9991-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 35 publications 35 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

2B11

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10670878

Product Specific Information

Description: The 2B11 monoclonal antibody reacts with mouse and human CXCR4, also known as CD184, Fusin, LESTR, or HUMSTR. Fusin is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a coreceptor for X4 HIV-1 entry into CD4+ cells. In addition it has been shown to play a role in lymphocyte migration. The ligand for CXCR4 is SDF1. CXCR4 is required for maintenance of granulocyte progenitors in the bone marrow and essential for B cell development. Expression of CXCR4 is different between mice and human. In the thymus CXCR4 is restricted to CD4+CD8+ cells while in the spleen predominant expression is found on B lymphocytes.

The monoclonal antibody 2B11 recognizes the amino terminus of CXCR4 and has been reported to have functional activity; it does not block SDF1a but causes internalization of the receptor.

Applications Reported: This 2B11 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 2B11 antibody has been tested by flow cytometric analysis of mouse thymocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CXCR4 (Fusin, LESTR, HUMSTR) is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a co-receptor for X4 HIV-1 entry into CD4+ cells. CXCR4 has been proved to be the co-receptor for HIV-2's binding to CD4 through envelope glycoprotein gp 120 and promotes Env-mediated fusion of the virus. In other cases, CXCR4 can even function as the only receptor for HIV-2's binding to the CD4 host cells. CXCR4 is the receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. CXCR4 acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Also, CXCR4 is involved in hematopoiesis and in cardiac ventricular septum formation, and plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. In the CNS, CXCR4 could mediate hippocampal-neuron survival and is involved in cerebellar development. CXCR4 binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Designing Synthetic, Sulfated Glycosaminoglycan Mimetics That Are Orally Bioavailable and Exhibiting In Vivo Anticancer Activity.

Journal of medicinal chemistry

Morla S,Ravikumar O,O'Hara C,Boothello R,Vera A,Abdelfadiel EI,Fayyad R,Afosah DK,Sharon C,Fernandez L,Shah SA,Patel BB,Desai UR

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 17-9991-82) in Flow Cytometry

Thu Jan 26 00:00:00 EST 2023

PHGDH is required for germinal center formation and is a therapeutic target in MYC-driven lymphoma.

The Journal of clinical investigation

D'Avola A,Legrave N,Tajan M,Chakravarty P,Shearer RL,King HW,Kluckova K,Cheung EC,Clear AJ,Gunawan AS,Zhang L,James LK,MacRae JI,Gribben JG,Calado DP,Vousden KH,Riches JC

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 17-9991-82) in Flow Cytometry

Mon May 02 00:00:00 EDT 2022

Smc3 dosage regulates B cell transit through germinal centers and restricts their malignant transformation.

Nature immunology

Rivas MA,Meydan C,Chin CR,Challman MF,Kim D,Bhinder B,Kloetgen A,Viny AD,Teater MR,McNally DR,Doane AS,Béguelin W,Fernández MTC,Shen H,Wang X,Levine RL,Chen Z,Tsirigos A,Elemento O,Mason CE,Melnick AM

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 17-9991-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

MIF but not MIF-2 recruits inflammatory macrophages in an experimental polymicrobial sepsis model.

The Journal of clinical investigation

Tilstam PV,Schulte W,Holowka T,Kim BS,Nouws J,Sauler M,Piecychna M,Pantouris G,Lolis E,Leng L,Bernhagen J,Fingerle-Rowson G,Bucala R

17-9991-82 was used in flow cytometry to study Mif-/- and Mif-2-/- mice to polymicrobial sepsis and observed a survival benefit with Mif but not Mif-2 deficiency.

Wed Dec 01 00:00:00 EST 2021

SARS-CoV-2 mRNA Vaccines Foster Potent Antigen-Specific Germinal Center Responses Associated with Neutralizing Antibody Generation.

Immunity

Lederer K,Castaño D,Gómez Atria D,Oguin TH,Wang S,Manzoni TB,Muramatsu H,Hogan MJ,Amanat F,Cherubin P,Lundgreen KA,Tam YK,Fan SHY,Eisenlohr LC,Maillard I,Weissman D,Bates P,Krammer F,Sempowski GD,Pardi N,Locci M

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 17-9991-82) in Flow Cytometry

Tue Dec 15 00:00:00 EST 2020

Cofilin1-driven actin dynamics controls migration of thymocytes and is essential for positive selection in the thymus.

Journal of cell science

Salz A,Gurniak C,Jönsson F,Witke W

17-9991 was used in Flow cytometry/Cell sorting to describe a novel role of cofilin1 in the physiological context of migration-dependent cell differentiation.

Fri Mar 06 00:00:00 EST 2020

Inhibition of Migration, Invasion and Drug Resistance of Pancreatic Adenocarcinoma Cells - Role of Snail, Slug and Twist and Small Molecule Inhibitors.

OncoTargets and therapy

Kaşıkcı E,Aydemir E,Bayrak ÖF,Şahin F

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 17-9991-82) in Flow Cytometry

Fri May 20 00:00:00 EDT 2022

Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production.

Journal of visualized experiments : JoVE

Upadhye A,Marshall M,Garmey JC,Bender TP,McNamara C

17-9991 was used in Flow cytometry/Cell sorting to describe a method to target B-1a cell migration to the bone marrow by retroviral-mediated overexpression of the C-X-C motif chemokine receptor 4 (CXCR4).

Sun May 31 00:00:00 EDT 2020

Enhanced germinal center reaction by targeting vaccine antigen to major histocompatibility complex class II molecules.

NPJ vaccines

Andersen TK,Huszthy PC,Gopalakrishnan RP,Jacobsen JT,Fauskanger M,Tveita AA,Grødeland G,Bogen B

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 17-9991-82) in Flow Cytometry

Thu Oct 05 00:00:00 EDT 2023

Bone-derived Nestin-positive mesenchymal stem cells improve cardiac function via recruiting cardiac endothelial cells after myocardial infarction.

Stem cell research & therapy

Lu D,Liao Y,Zhu SH,Chen QC,Xie DM,Liao JJ,Feng X,Jiang MH,He W

17-9991 was used in Flow cytometry/Cell sorting to show that Nestin is a useful marker for the identification of functional BMSCs and indicate that Nestin+ BMSCs could be a better therapeutic candidate for cardiac repair.

Sat Apr 27 00:00:00 EDT 2019

Lsd1 regulates skeletal muscle regeneration and directs the fate of satellite cells.

Nature communications

Tosic M,Allen A,Willmann D,Lepper C,Kim J,Duteil D,Schüle R

17-9991 was used in Flow cytometry/Cell sorting to show that elevated levels of lysine-specific demethylase 1 have a beneficial effect on muscle regeneration and recovery after injury.

Thu Jan 25 00:00:00 EST 2018

Drp1 Controls Effective T Cell Immune-Surveillance by Regulating T Cell Migration, Proliferation, and cMyc-Dependent Metabolic Reprogramming.

Cell reports

Simula L,Pacella I,Colamatteo A,Procaccini C,Cancila V,Bordi M,Tregnago C,Corrado M,Pigazzi M,Barnaba V,Tripodo C,Matarese G,Piconese S,Campello S

17-9991 was used in Flow cytometry/Cell sorting to describe the crucial role of the mitochondrial protein Drp1 in T cell development and regulation of lymphocyte immune-surveillance.

Tue Dec 11 00:00:00 EST 2018

High-affinity IgM⁺ memory B cells are defective in differentiation into IgM antibody-secreting cells by re-stimulation with a T cell-dependent antigen.

Scientific reports

Tashiro Y,Murakami A,Hara Y,Shimizu T,Kubo M,Goitsuka R,Kishimoto H,Azuma T

17-9991 was used in Flow cytometry/Cell sorting to demonstrate that only non-germinal centre-dependent IgM+ memomry B cells have terminal differential potential to become plasma cell during recall immune response.

Fri Sep 28 00:00:00 EDT 2018

Convective forces increase CXCR4-dependent glioblastoma cell invasion in GL261 murine model.

Scientific reports

Cornelison RC,Brennan CE,Kingsmore KM,Munson JM

17-9991 was used in Flow cytometry/Cell sorting to investigate whether convective flow alters glioma invasion in a syngeneic GL261 mouse model of glioblastoma.

Mon Nov 19 00:00:00 EST 2018

Loss of mDia1 causes neutropenia via attenuated CD11b endocytosis and increased neutrophil adhesion to the endothelium.

Blood advances

Mei Y,Feng G,Rahimi N,Zhao B,Zhang J,Cao L,Yang J,Gao J,Chen Y,Sumagin R,Muller WA,Zhang L,Ji P

17-9991 was used in Flow cytometry/Cell sorting to investigate if mDia1 knockout mice show cell-autonomously increased CD11b expression on neutrophils in the peripheral blood and bone marrow.

Tue Sep 12 00:00:00 EDT 2017

A Functional Bioluminescent Zebrafish Screen for Enhancing Hematopoietic Cell Homing.

Stem cell reports

Astuti Y,Kramer AC,Blake AL,Blazar BR,Tolar J,Taisto ME,Lund TC

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 17-9991-82) in Flow Cytometry

Tue Jan 10 00:00:00 EST 2017

Molecular basis for the antagonistic activity of an anti-CXCR4 antibody.

mAbs

Peng L,Damschroder MM,Cook KE,Wu H,Dall'Acqua WF

17-9991 was used in Flow cytometry/Cell sorting to characterise the mechanism of action at a molecular level of a potent anti-C-X-C chemokine receptor 4 antagonistic antibody.

Fri Sep 30 00:00:00 EDT 2016

Role of G-CSF in monophosphoryl lipid A-mediated augmentation of neutrophil functions after burn injury.

Journal of leukocyte biology

Bohannon JK,Luan L,Hernandez A,Afzal A,Guo Y,Patil NK,Fensterheim B,Sherwood ER

17-9991 was used in Flow cytometry/Cell sorting to provide evidence that monophosphoryl lipid A-induced G-CSF facilitates a robust immune response via neutrophils to the site of infection after burn injury.

Fri Apr 01 00:00:00 EDT 2016

G-CSF and Neutrophils Are Nonredundant Mediators of Murine Experimental Autoimmune Uveoretinitis.

The American journal of pathology

Goldberg GL,Cornish AL,Murphy J,Pang ES,Lim LL,Campbell IK,Scalzo-Inguanti K,Chen X,McMenamin PG,Maraskovsky E,McKenzie BS,Wicks IP

17999182 was used in flow cytometry to determine the role of G-CSF in a murine model of human uveitis-experimental autoimmune uveoretinitis

Fri Jan 01 00:00:00 EST 2016

Induced Pluripotent Stem Cells derived Muscle Progenitors Effectively Mitigate Muscular Dystrophy through Restoring the Dystrophin Distribution.

Journal of stem cell research & therapy

Cai WF,Huang W,Wang L,Wang JP,Zhang L,Ashraf M,Wu S,Wang Y

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 17-9991-82) in Flow Cytometry

Wed Sep 30 00:00:00 EDT 2020

Inflammation rapidly reorganizes mouse bone marrow B cells and their environment in conjunction with early IgM responses.

Blood

Moreau JM,Berger A,Nelles ME,Mielnik M,Furlonger C,Cen SY,Besla R,Robbins CS,Paige CJ

17-9991 was used in Flow cytometry/Cell sorting to study the rapidity with which peripheral inflammation modifies the bone marrow compartment, and demonstrate that such modifications regulate acute IgM production within this organ.

Thu Sep 03 00:00:00 EDT 2015

CXCR4 expression on pathogenic T cells facilitates their bone marrow infiltration in a mouse model of aplastic anemia.

Blood

Arieta Kuksin C,Gonzalez-Perez G,Minter LM

17-9991 was used in Flow cytometry/Cell sorting to demonstrate that CXCR4 mediates migration of pathogenic T-cells to the bone marrow in aplastic anemia mice.

Thu Mar 26 00:00:00 EDT 2015

Function-oriented development of CXCR4 antagonists as selective human immunodeficiency virus (HIV)-1 entry inhibitors.

Journal of medicinal chemistry

Wu CH,Wang CJ,Chang CP,Cheng YC,Song JS,Jan JJ,Chou MC,Ke YY,Ma J,Wong YC,Hsieh TC,Tien YC,Gullen EA,Lo CF,Cheng CY,Liu YW,Sadani AA,Tsai CH,Hsieh HP,Tsou LK,Shia KS

17-9991 was used in Flow cytometry/Cell sorting to identify a CXCR4 antagonist for anti-HIV therapeutics, producing a purine-based antagonist, compound 24, with a subnanomolar EC50 value.

Thu Feb 12 00:00:00 EST 2015

Inhibition of insulin-like growth factor receptor/AKT/mammalian target of rapamycin axis targets colorectal cancer stem cells by attenuating mevalonate-isoprenoid pathway in vitro and in vivo.

Oncotarget

Sharon C,Baranwal S,Patel NJ,Rodriguez-Agudo D,Pandak WM,Majumdar AP,Krystal G,Patel BB

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 17-9991-82) in Flow Cytometry

Sat Jun 20 00:00:00 EDT 2015

Intravenous transplantation of very small embryonic like stem cells in treatment of diabetes mellitus.

Avicenna journal of medical biotechnology

Abouzaripour M,Ragerdi Kashani I,Pasbakhsh P,Atlasy N

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 17-9991-82) in Flow Cytometry

Thu Apr 30 00:00:00 EDT 2015

Systemic high-mobility group box 1 administration suppresses skin inflammation by inducing an accumulation of PDGFRα(+) mesenchymal cells from bone marrow.

Scientific reports

Aikawa E,Fujita R,Kikuchi Y,Kaneda Y,Tamai K

17-9991 was used in Flow cytometry/Cell sorting to investigate the function of HMGB1-mobilised mesenchymal cells in the regeneration of mouse skin grafts.

Fri Jun 05 00:00:00 EDT 2015

Histone deacetylation promotes mouse neural induction by restricting Nodal-dependent mesendoderm fate.

Nature communications

Liu P,Dou X,Liu C,Wang L,Xing C,Peng G,Chen J,Yu F,Qiao Y,Song L,Wu Y,Yue C,Li J,Han JD,Tang K,Jing N

17-9991 was used in Flow cytometry/Cell sorting to demonstrate that epigenetic histone deacetylation ensures neural fate commitment by restricting Nodal signalling.

Thu Apr 23 00:00:00 EDT 2015

Infection-induced type I interferons activate CD11b on B-1 cells for subsequent lymph node accumulation.

Nature communications

Waffarn EE,Hastey CJ,Dixit N,Soo Choi Y,Cherry S,Kalinke U,Simon SI,Baumgarth N

17-9991 was used in Flow cytometry/Cell sorting to demonstrate that influenza infection-induced type I interferons directly stimulate body cavity B-1 cells.

Fri Nov 27 00:00:00 EST 2015

POL5551, a novel and potent CXCR4 antagonist, enhances sensitivity to chemotherapy in pediatric ALL.

Oncotarget

Sison EA,Magoon D,Li L,Annesley CE,Romagnoli B,Douglas GJ,Tuffin G,Zimmermann J,Brown P

17-9991 was used in Flow cytometry/Cell sorting to assess the potential for POL5551 to improve outcomes in children with high-risk acute lymphoblastic leukaemia.

Tue Oct 13 00:00:00 EDT 2015

Fibrocytes in the fibrotic lung: altered phenotype detected by flow cytometry.

Frontiers in pharmacology

Reese C,Lee R,Bonner M,Perry B,Heywood J,Silver RM,Tourkina E,Visconti RP,Hoffman S

17-9991 was used in Flow cytometry/Cell sorting to show the distinctive phenotype of fibrotic lung fibrocytes suggests that fibrocyte differentiation occurs primarily within the target organ.

Mon Jul 07 00:00:00 EDT 2014

Plerixafor as a chemosensitizing agent in pediatric acute lymphoblastic leukemia: efficacy and potential mechanisms of resistance to CXCR4 inhibition.

Oncotarget

Sison EA,Magoon D,Li L,Annesley CE,Rau RE,Small D,Brown P

17-9991 was used in Flow cytometry/Cell sorting to demonstrate that cells which upregulate surface CXCR4 in response to chemotherapy treatment are protected from chemotherapy-induced apoptosis when co-cultured with bone marrow stroma.

Wed Oct 15 00:00:00 EDT 2014

Non-invasive imaging provides spatiotemporal information on disease progression and response to therapy in a murine model of multiple myeloma.

PloS one

Riedel SS,Mottok A,Brede C,Bäuerlein CA,Jordán Garrote AL,Ritz M,Mattenheimer K,Rosenwald A,Einsele H,Bogen B,Beilhack A

17-9991 was used in Flow cytometry/Cell sorting to describe a novel MM mouse model that facilitates the convenient, reliable, and sensitive tracking of myeloma cells with bioluminescence imaging.

Tue Jul 02 00:00:00 EDT 2013

Characterization of omental immune aggregates during establishment of a latent gammaherpesvirus infection.

PloS one

Gray KS,Collins CM,Speck SH

17-9991 was used in Flow cytometry/Cell sorting to investigate the role of the omentum in gammaherpesvirus pathogenesis during early MHV68 infection and long-term latency.

Tue Feb 19 00:00:00 EST 2013

Intramuscular VEGF activates an SDF1-dependent progenitor cell cascade and an SDF1-independent muscle paracrine cascade for cardiac repair.

American journal of physiology. Heart and circulatory physiology

Zisa D,Shabbir A,Mastri M,Taylor T,Aleksic I,McDaniel M,Suzuki G,Lee T

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 17-9991-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2011

Intramuscular VEGF repairs the failing heart: role of host-derived growth factors and mobilization of progenitor cells.

American journal of physiology. Regulatory, integrative and comparative physiology

Zisa D,Shabbir A,Mastri M,Suzuki G,Lee T

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 17-9991-82) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: C Cmotif chemokine; C X C motif chemokine; C-X-C chemokine receptor type 4; CC motif chemokine; CCmotif chemokine; CD antigen CD184; CD184; chemokine (C-X-C) receptor 4; chemokine receptor 4; CXC; CXC motif chemokine; CXC-R4; CXCR-4; Fusin; HUMSTR; LESTR; Leukocyte-derived seven transmembrane domain receptor; leukocyte-expressed seven-transmembrane-domain; PB-CKR; Pre-B-cell-derived chemokine receptor; SDF-1 receptor; Stromal cell-derived factor 1 receptor

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Gene Aliases: b2b220Clo; CD184; Cmkar4; Cxcr4; LESTR; PB-CKR; PBSF/SDF-1; Sdf1r

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UniProt ID: (Mouse) P70658

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Entrez Gene ID: (Mouse) 12767

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Function(s)

actin binding signal transducer activity G-protein coupled receptor activity chemokine receptor activity protein binding drug binding C-X-C chemokine receptor activity cytokine binding ubiquitin protein ligase binding myosin light chain binding ubiquitin binding

Process(es)

patterning of blood vessels ameboidal-type cell migration neuron migration epithelial cell development chemotaxis signal transduction G-protein coupled receptor signaling pathway germ cell development nervous system development brain development motor neuron axon guidance germ cell migration organ morphogenesis cell migration calcium-mediated signaling telencephalon cell migration regulation of cell migration T cell proliferation neutrophil activation regulation of programmed cell death myelin maintenance positive regulation of oligodendrocyte differentiation regulation of chemotaxis regulation of calcium ion transport cardiac muscle contraction chemokine-mediated signaling pathway cellular response to cytokine stimulus

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CD184 (CXCR4) Monoclonal Antibody (2B11), APC, eBioscience™

CD184 (CXCR4) Antibody (17-9991-82) in Flow

Product Details

17-9991-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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