Invitrogen

CD24 Monoclonal Antibody (eBioSN3 (SN3 A5-2H10)), PE-eFluor™ 610, eBioscience™

13 Published Figures

11 References

View all (96) CD24 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (13)

FIGURE: 1 / 14

CD24 Antibody (61-0247-42) in Flow

Normal human peripheral blood cells were stained with Mouse IgG1 kappa Isotype Control, PE-eFluor 610 (Product # 61-4714-82) (blue histogram) or CD24 Monoclonal Antibody, PE-eFluor 610 (purple histogram). Cells in the granulocyte gate were used for analysis.

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Product Details

61-0247-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 11 publications 11 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-eFluor™ 610, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioSN3 (SN3 A5-2H10)

Conjugate

PE-eFluor™ 610 PE-eFluor™ 610 PE-eFluor™ 610

Excitation/Emission Max

565/606 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2815292

Product Specific Information

Description: The SN3 monoclonal antibody reacts with the human CD24 molecule, also known as Heat Stable Antigen (HSA). This 35-50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes, and myeloid lineage. It has been reported that P-selectin (CD62P) binds to CD24.

Applications Reported: This eBioSN3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioSN3 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

PE-eFluor 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red). Please make sure that your instrument is capable of detecting this fluorochrome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Target Information

CD24 is a two-chain glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed at multiple stages of B-cell development, beginning with the bone marrow pro-B-cell compartment and continuing through mature, surface Ig positive B-cells. Plasma cell expression is very low or negative. CD24 is also expressed on the majority of B-lineage acute lymphoblastic leukemias, B-cell CCLs and B-cell non-Hodgkin's lymphomas. CD24 may play a role in regulation of B-cell proliferation and maturation, and control of autoimmunity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Immune Checkpoint Profiling in Humanized Breast Cancer Mice Revealed Cell-Specific LAG-3/PD-1/TIM-3 Co-Expression and Elevated PD-1/TIM-3 Secretion.

Cancers

Bruss C,Kellner K,Albert V,Hutchinson JA,Seitz S,Ortmann O,Brockhoff G,Wege AK

Published figure using CD24 monoclonal antibody (Product # 61-0247-42) in Flow Cytometry

Thu May 04 00:00:00 EDT 2023

miR‑122/SENP1 axis confers stemness and chemoresistance to liver cancer through Wnt/β‑catenin signaling.

Oncology letters

Dai J,Hao Y,Chen X,Yu Q,Wang B

Published figure using CD24 monoclonal antibody (Product # 61-0247-42) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2023

Naringin protects human nucleus pulposus cells against TNF-α-induced inflammation, oxidative stress, and loss of cellular homeostasis by enhancing autophagic flux via AMPK/SIRT1 activation.

Oxidative medicine and cellular longevity

Chen R,Gao S,Guan H,Zhang X,Gao Y,Su Y,Song Y,Jiang Y,Li N

Published figure using CD24 monoclonal antibody (Product # 61-0247-42) in Flow Cytometry

Thu Mar 24 00:00:00 EDT 2022

Elevated transcription and glycosylation of B3GNT5 promotes breast cancer aggressiveness.

Journal of experimental & clinical cancer research : CR

Miao Z,Cao Q,Liao R,Chen X,Li X,Bai L,Ma C,Deng X,Dai Z,Li J,Dong C

Published figure using CD24 monoclonal antibody (Product # 61-0247-42) in Flow Cytometry

Sat May 07 00:00:00 EDT 2022

Alterations in T and B cell function persist in convalescent COVID-19 patients.

Med (New York, N.Y.)

Shuwa HA,Shaw TN,Knight SB,Wemyss K,McClure FA,Pearmain L,Prise I,Jagger C,Morgan DJ,Khan S,Brand O,Mann ER,Ustianowski A,Bakerly ND,Dark P,Brightling CE,Brij S,Felton T,Simpson A,Grainger JR,Hussell T,Konkel JE,Menon M

Published figure using CD24 monoclonal antibody (Product # 61-0247-42) in Flow Cytometry

Fri Jun 11 00:00:00 EDT 2021

A CD10-OGP Membrane Peptolytic Signaling Axis in Fibroblasts Regulates Lipid Metabolism of Cancer Stem Cells via SCD1.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Yu S,Lu Y,Su A,Chen J,Li J,Zhou B,Liu X,Xia Q,Li Y,Li J,Huang M,Ye Y,Zhao Q,Jiang S,Yan X,Wang X,Di C,Pan J,Su S

Published figure using CD24 monoclonal antibody (Product # 61-0247-42) in Flow Cytometry

Fri Oct 01 00:00:00 EDT 2021

Mucosal-associated invariant T cell responses differ by sex in COVID-19.

Med (New York, N.Y.)

Yu C,Littleton S,Giroux NS,Mathew R,Ding S,Kalnitsky J,Yang Y,Petzold E,Chung HA,Rivera GO,Rotstein T,Xi R,Ko ER,Tsalik EL,Sempowski GD,Denny TN,Burke TW,McClain MT,Woods CW,Shen X,Saban DR

61-0247-42 was used in Flow Cytometry to reveal a pronounced reduction of circulating mucosal-associated invariant T (MAIT) cells in infected females.

Fri Jun 11 00:00:00 EDT 2021

IGF2BP2 regulates DANCR by serving as an N6-methyladenosine reader.

Cell death and differentiation

Hu X,Peng WX,Zhou H,Jiang J,Zhou X,Huang D,Mo YY,Yang L

Published figure using CD24 monoclonal antibody (Product # 61-0247-42) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2020

Obesity-Altered Adipose Stem Cells Promote Radiation Resistance of Estrogen Receptor Positive Breast Cancer through Paracrine Signaling.

International journal of molecular sciences

Sabol RA,Villela VA,Denys A,Freeman BT,Hartono AB,Wise RM,Harrison MAA,Sandler MB,Hossain F,Miele L,Bunnell BA

Published figure using CD24 monoclonal antibody (Product # 61-0247-42) in Flow Cytometry

Wed Apr 15 00:00:00 EDT 2020

BRCA1 regulates the cancer stem cell fate of breast cancer cells in the context of hypoxia and histone deacetylase inhibitors.

Scientific reports

Kim H,Lin Q,Yun Z

Published figure using CD24 monoclonal antibody (Product # 61-0247-42) in Flow Cytometry

Thu Jul 04 00:00:00 EDT 2019

The hypoxic tumor microenvironment in vivo selects the cancer stem cell fate of breast cancer cells.

Breast cancer research : BCR

Kim H,Lin Q,Glazer PM,Yun Z

Published figure using CD24 monoclonal antibody (Product # 61-0247-42) in Flow Cytometry

Tue Mar 06 00:00:00 EST 2018

Bioinformatics

Protein Aliases: CD24; CD24 antigen (small cell lung carcinoma cluster 4 antigen); FLJ22950; FLJ43543; MGC75043; Signal transducer CD24; Small cell lung carcinoma cluster 4 antigen

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Gene Aliases: CD24; CD24A

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UniProt ID: (Human) P25063

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Entrez Gene ID: (Human) 100133941

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Function(s)

signal transducer activity protein binding protein kinase binding protein tyrosine kinase activator activity

Process(es)

response to hypoxia cell activation regulation of cytokine-mediated signaling pathway response to molecule of bacterial origin immune response-regulating cell surface receptor signaling pathway positive regulation of cytosolic calcium ion concentration Wnt signaling pathway single organismal cell-cell adhesion cell migration regulation of epithelial cell differentiation T cell costimulation B cell receptor transport into membrane raft chemokine receptor transport out of membrane raft negative regulation of transforming growth factor beta3 production positive regulation of activated T cell proliferation regulation of phosphorylation cholesterol homeostasis positive regulation of MAP kinase activity regulation of MAPK cascade response to estrogen respiratory burst positive regulation of protein tyrosine kinase activity glomerular visceral epithelial cell differentiation glomerular parietal epithelial cell differentiation intrinsic apoptotic signaling pathway positive regulation of nephron tubule epithelial cell differentiation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD24 Monoclonal Antibody (eBioSN3 (SN3 A5-2H10)), PE-eFluor™ 610, eBioscience™

CD24 Antibody (61-0247-42) in Flow

Product Details

61-0247-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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