Invitrogen

CD309 (FLK1) Monoclonal Antibody (Avas12a1), Biotin, eBioscience™

47 Published Figures

53 References

View all (71) FLK1 (VEGF Receptor 2) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (47)

CD309 (FLK1) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 48

CD309 (FLK1) Antibody (13-5821-82) in Flow

Staining of bEnd-3 cell line with Anti-Mouse CD309 (FLK1) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

CD309 (FLK1) Antibody in Western Blot, Flow Cytometry (WB, Flow)

CD309 (FLK1) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

CD309 (FLK1) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CD309 (FLK1) Antibody in Immunohistochemistry (IHC)

CD309 (FLK1) Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)

CD309 (FLK1) Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

CD309 (FLK1) Antibody in Flow Cytometry, Peptide array (Flow, ARRAY)

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Product Details

13-5821-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

0.5 µg/test

View 32 publications 32 publications

Functional Assay (Functional)

View 1 publication 1 publication

Peptide Array (Array)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 7 publications 7 publications

Product Specifications

Species Reactivity

Mouse

Published species

Dog, Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

Avas12a1

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466711

Product Specific Information

Description: The Avas12a1 monoclonal antibody reacts with mouse Flk-1, also known as vascular endothelial growth factor receptor 2 (VEGFR2). Flk-1 is a receptor tyrosine kinase involved in vascular endothelial tissue development and is expressed on endothelial cells during embryonic stages and some endothelial tissues in the adult.

Applications Reported: The Avas12a1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The Avas12a1 antibody has been tested by flow cytometric analysis of bEnd.3 cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

KDR (FLK1, VEGFR2) is a type III receptor tyrosine kinase. It binds vascular endothelial growth factor with high affinity in vitro, and is expressed early in development by endothelial cell precursors. FLK1 (VEGF Receptor 2) encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc. Mutations of FLK1 (VEGF Receptor 2) are implicated in infantile capillary hemangiomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A genome-wide relay of signalling-responsive enhancers drives hematopoietic specification.

Nature communications

Edginton-White B,Maytum A,Kellaway SG,Goode DK,Keane P,Pagnuco I,Assi SA,Ames L,Clarke M,Cockerill PN,Göttgens B,Cazier JB,Bonifer C

13-5821-82 was used in Magnetic cell separation to provide an important resource for studies of hematopoietic specification and highlights the mechanisms of how and where extrinsic signals program a cell type-specific chromatin landscape driving hematopoietic differentiation.

Tue Jan 17 00:00:00 EST 2023

Lamin A/C-dependent chromatin architecture safeguards naïve pluripotency to prevent aberrant cardiovascular cell fate and function.

Nature communications

Wang Y,Elsherbiny A,Kessler L,Cordero J,Shi H,Serke H,Lityagina O,Trogisch FA,Mohammadi MM,El-Battrawy I,Backs J,Wieland T,Heineke J,Dobreva G

Published figure using CD309 (FLK1) monoclonal antibody (Product # 13-5821-82) in Flow Cytometry

Fri Nov 04 00:00:00 EDT 2022

Upregulation of Extracellular Vesicles-Encapsulated miR-132 Released From Mesenchymal Stem Cells Attenuates Ischemic Neuronal Injury by Inhibiting Smad2/c-jun Pathway via Acvr2b Suppression.

Frontiers in cell and developmental biology

Feng B,Meng L,Luan L,Fang Z,Zhao P,Zhao G

13-5821 was used in Flow cytometry/Cell sorting to demonstrate that activin receptor type IIB expression is inhibited by miR-132 from mesenchymal stem cells which attenuates ischemic neuronal injury.

Fri Mar 26 00:00:00 EDT 2021

SCL/TAL1 cooperates with Polycomb RYBP-PRC1 to suppress alternative lineages in blood-fated cells.

Nature communications

Chagraoui H,Kristiansen MS,Ruiz JP,Serra-Barros A,Richter J,Hall-Ponselé E,Gray N,Waithe D,Clark K,Hublitz P,Repapi E,Otto G,Sopp P,Taylor S,Thongjuea S,Vyas P,Porcher C

Published figure using CD309 (FLK1) monoclonal antibody (Product # 13-5821-82) in Flow Cytometry

Tue Dec 18 00:00:00 EST 2018

Single-cell transcriptomics reveals a new dynamical function of transcription factors during embryonic hematopoiesis.

eLife

Bergiers I,Andrews T,Vargel Bölükbaşı Ö,Buness A,Janosz E,Lopez-Anguita N,Ganter K,Kosim K,Celen C,Itır Perçin G,Collier P,Baying B,Benes V,Hemberg M,Lancrin C

Published figure using CD309 (FLK1) monoclonal antibody (Product # 13-5821-82) in Flow Cytometry

Tue Mar 20 00:00:00 EDT 2018

Single-cell transcriptomics reveals a new dynamical function of transcription factors during embryonic hematopoiesis.

eLife

Bergiers I,Andrews T,Vargel Bölükbaşı Ö,Buness A,Janosz E,Lopez-Anguita N,Ganter K,Kosim K,Celen C,Itır Perçin G,Collier P,Baying B,Benes V,Hemberg M,Lancrin C

Published figure using CD309 (FLK1) monoclonal antibody (Product # 13-5821-82) in Flow Cytometry

Tue Mar 20 00:00:00 EDT 2018

LMO2 is required for TAL1 DNA binding activity and initiation of definitive haematopoiesis at the haemangioblast stage.

Nucleic acids research

Stanulovic VS,Cauchy P,Assi SA,Hoogenkamp M

13-5821-82 was used in Magnetic cell separation to show that Lmo2-/- mouse embryonic stem cells differentiated to Flk-1+ haemangioblasts, but less efficiently to haemogenic endothelium.

Fri Sep 29 00:00:00 EDT 2017

Sustained inflammation after pericyte depletion induces irreversible blood-retina barrier breakdown.

JCI insight

Ogura S,Kurata K,Hattori Y,Takase H,Ishiguro-Oonuma T,Hwang Y,Ahn S,Park I,Ikeda W,Kusuhara S,Fukushima Y,Nara H,Sakai H,Fujiwara T,Matsushita J,Ema M,Hirashima M,Minami T,Shibuya M,Takakura N,Kim P,Miyata T,Ogura Y,Uemura A

Published figure using CD309 (FLK1) monoclonal antibody (Product # 13-5821-82) in Immunohistochemistry

Thu Feb 09 00:00:00 EST 2017

Antifibrotic role of vascular endothelial growth factor in pulmonary fibrosis.

JCI insight

Murray LA,Habiel DM,Hohmann M,Camelo A,Shang H,Zhou Y,Coelho AL,Peng X,Gulati M,Crestani B,Sleeman MA,Mustelin T,Moore MW,Ryu C,Osafo-Addo AD,Elias JA,Lee CG,Hu B,Herazo-Maya JD,Knight DA,Hogaboam CM,Herzog EL

Published figure using CD309 (FLK1) monoclonal antibody (Product # 13-5821-82) in Flow Cytometry

Thu Aug 17 00:00:00 EDT 2017

Mesp1 controls the speed, polarity, and directionality of cardiovascular progenitor migration.

The Journal of cell biology

Chiapparo G,Lin X,Lescroart F,Chabab S,Paulissen C,Pitisci L,Bondue A,Blanpain C

13-5821 was used in Flow cytometry/Cell sorting to identify the molecular interface controlled by Mesp1 that links CP specification and cell migration.

Mon May 23 00:00:00 EDT 2016

Endothelial cell-anchored tissue factor pathway inhibitor regulates tumor metastasis to the lung in mice.

Molecular carcinogenesis

Wang J,Xiao J,Wen D,Wu X,Mao Z,Zhang J,Ma D

Published figure using CD309 (FLK1) monoclonal antibody (Product # 13-5821-82) in Immunohistochemistry (Paraffin)

Sun May 01 00:00:00 EDT 2016

Small Molecule Inhibitor of CBFβ-RUNX Binding for RUNX Transcription Factor Driven Cancers.

EBioMedicine

Illendula A,Gilmour J,Grembecka J,Tirumala VSS,Boulton A,Kuntimaddi A,Schmidt C,Wang L,Pulikkan JA,Zong H,Parlak M,Kuscu C,Pickin A,Zhou Y,Gao Y,Mishra L,Adli M,Castilla LH,Rajewski RA,Janes KA,Guzman ML,Bonifer C,Bushweller JH

13-5821 was used in Magnetic cell separation to investigate whether targeting RUNX proteins could have broad utility in cancer therapeutics.

Wed Jun 01 00:00:00 EDT 2016

The Isl1/Ldb1 Complex Orchestrates Genome-wide Chromatin Organization to Instruct Differentiation of Multipotent Cardiac Progenitors.

Cell stem cell

Caputo L,Witzel HR,Kolovos P,Cheedipudi S,Looso M,Mylona A,van IJcken WF,Laugwitz KL,Evans SM,Braun T,Soler E,Grosveld F,Dobreva G

Published figure using CD309 (FLK1) monoclonal antibody (Product # 13-5821-82) in Flow Cytometry

Thu Sep 03 00:00:00 EDT 2015

FOXF1 inhibits hematopoietic lineage commitment during early mesoderm specification.

Development (Cambridge, England)

Fleury M,Eliades A,Carlsson P,Lacaud G,Kouskoff V

13-5821 was used in Flow cytometry/Cell sorting to investigate the lineage specification of early mesodermal precursors expressing or not the Forkhead transcription factor FOXF1.

Thu Oct 01 00:00:00 EDT 2015

Histone Demethylases KDM4A and KDM4C Regulate Differentiation of Embryonic Stem Cells to Endothelial Cells.

Stem cell reports

Wu L,Wary KK,Revskoy S,Gao X,Tsang K,Komarova YA,Rehman J,Malik AB

Published figure using CD309 (FLK1) monoclonal antibody (Product # 13-5821-82) in Flow Cytometry

Tue Jul 14 00:00:00 EDT 2015

Developmental-stage-dependent transcriptional response to leukaemic oncogene expression.

Nature communications

Regha K,Assi SA,Tsoulaki O,Gilmour J,Lacaud G,Bonifer C

13-5821 was used in Magnetic cell separation to study the differentiation of mouse embryonic stem cells, expressing an inducible RUNX1-ETO gene, into blood cells.

Thu May 28 00:00:00 EDT 2015

Ultrasound molecular imaging of vascular endothelial growth factor receptor 2 expression for endometrial receptivity evaluation.

Theranostics

Liu H,Chen Y,Yan F,Han X,Wu J,Liu X,Zheng H

13-5821 was used as a Control in experiments to showcase a novel method to assess endometrial receptivity for enhancing in-vitro fertilisation success rates.

Mon Aug 31 00:00:00 EDT 2015

Mesenchymal-endothelial transition contributes to cardiac neovascularization.

Nature

Ubil E,Duan J,Pillai IC,Rosa-Garrido M,Wu Y,Bargiacchi F,Lu Y,Stanbouly S,Huang J,Rojas M,Vondriska TM,Stefani E,Deb A

Published figure using CD309 (FLK1) monoclonal antibody (Product # 13-5821-82) in Flow Cytometry

Thu Oct 30 00:00:00 EDT 2014

The miR-126-VEGFR2 axis controls the innate response to pathogen-associated nucleic acids.

Nature immunology

Agudo J,Ruzo A,Tung N,Salmon H,Leboeuf M,Hashimoto D,Becker C,Garrett-Sinha LA,Baccarini A,Merad M,Brown BD

13-5821 was used in Flow cytometry/Cell sorting to identify the miR-126-VEGFR2 axis as an important regulator of the innate response that operates through multiscale control of pDCs.

Wed Jan 01 00:00:00 EST 2014

SOX9 accelerates ESC differentiation to three germ layer lineages by repressing SOX2 expression through P21 (WAF1/CIP1).

Development (Cambridge, England)

Yamamizu K,Schlessinger D,Ko MS

Published figure using CD309 (FLK1) monoclonal antibody (Product # 13-5821-82) in Flow Cytometry

Sat Nov 01 00:00:00 EDT 2014

SOX9 accelerates ESC differentiation to three germ layer lineages by repressing SOX2 expression through P21 (WAF1/CIP1).

Development (Cambridge, England)

Yamamizu K,Schlessinger D,Ko MS

Published figure using CD309 (FLK1) monoclonal antibody (Product # 13-5821-82) in Flow Cytometry

Sat Nov 01 00:00:00 EDT 2014

Expression of podocalyxin separates the hematopoietic and vascular potentials of mouse embryonic stem cell-derived mesoderm.

Stem cells (Dayton, Ohio)

Zhang H,Nieves JL,Fraser ST,Isern J,Douvaras P,Papatsenko D,D'Souza SL,Lemischka IR,Dyer MA,Baron MH

Published figure using CD309 (FLK1) monoclonal antibody (Product # 13-5821-82) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2014

Cooperative interaction of Etv2 and Gata2 regulates the development of endothelial and hematopoietic lineages.

Developmental biology

Shi X,Richard J,Zirbes KM,Gong W,Lin G,Kyba M,Thomson JA,Koyano-Nakagawa N,Garry DJ

Published figure using CD309 (FLK1) monoclonal antibody (Product # 13-5821-82) in Flow Cytometry

Thu May 15 00:00:00 EDT 2014

Dual modulation of the mitochondrial permeability transition pore and redox signaling synergistically promotes cardiomyocyte differentiation from pluripotent stem cells.

Journal of the American Heart Association

Cho SW,Park JS,Heo HJ,Park SW,Song S,Kim I,Han YM,Yamashita JK,Youm JB,Han J,Koh GY

13-5821 was used in Magnetic cell separation to show that mPTP inhibition by CsA alters mitochondrial oxidative metabolism and redox signaling.

Thu Mar 13 00:00:00 EDT 2014

Specification of chondrocytes and cartilage tissues from embryonic stem cells.

Development (Cambridge, England)

Craft AM,Ahmed N,Rockel JS,Baht GS,Alman BA,Kandel RA,Grigoriadis AE,Keller GM

13-5821 was used in Flow cytometry/Cell sorting to establish the developmental program for chondrogenic mesoderm formation from mouse embryonic stem cells.

Sat Jun 01 00:00:00 EDT 2013

Nkx2-5 mediates differential cardiac differentiation through interaction with Hoxa10.

Stem cells and development

Behrens AN,Iacovino M,Lohr JL,Ren Y,Zierold C,Harvey RP,Kyba M,Garry DJ,Martin CM

Published figure using CD309 (FLK1) monoclonal antibody (Product # 13-5821-82) in Flow Cytometry

Thu Aug 01 00:00:00 EDT 2013

Nkx2-5 mediates differential cardiac differentiation through interaction with Hoxa10.

Stem cells and development

Behrens AN,Iacovino M,Lohr JL,Ren Y,Zierold C,Harvey RP,Kyba M,Garry DJ,Martin CM

Published figure using CD309 (FLK1) monoclonal antibody (Product # 13-5821-82) in Flow Cytometry

Thu Aug 01 00:00:00 EDT 2013

Etv2 rescues Flk1 mutant embryoid bodies.

Genesis (New York, N.Y. : 2000)

Rasmussen TL,Martin CM,Walter CA,Shi X,Perlingeiro R,Koyano-Nakagawa N,Garry DJ

Published figure using CD309 (FLK1) monoclonal antibody (Product # 13-5821-82) in Flow Cytometry

Mon Jul 01 00:00:00 EDT 2013

Etv2 rescues Flk1 mutant embryoid bodies.

Genesis (New York, N.Y. : 2000)

Rasmussen TL,Martin CM,Walter CA,Shi X,Perlingeiro R,Koyano-Nakagawa N,Garry DJ

Published figure using CD309 (FLK1) monoclonal antibody (Product # 13-5821-82) in Flow Cytometry

Mon Jul 01 00:00:00 EDT 2013

DNA methylation restricts lineage-specific functions of transcription factor Gata4 during embryonic stem cell differentiation.

PLoS genetics

Oda M,Kumaki Y,Shigeta M,Jakt LM,Matsuoka C,Yamagiwa A,Niwa H,Okano M

13-5821 was used in Magnetic cell separation to study DNA methylation during embryonic stem cell differentiation.

Sat Jun 01 00:00:00 EDT 2013

DNA methylation restricts lineage-specific functions of transcription factor Gata4 during embryonic stem cell differentiation.

PLoS genetics

Oda M,Kumaki Y,Shigeta M,Jakt LM,Matsuoka C,Yamagiwa A,Niwa H,Okano M

13-5821 was used in Magnetic cell separation to study DNA methylation during embryonic stem cell differentiation.

Sat Jun 01 00:00:00 EDT 2013

Imaging of thyroid tumor angiogenesis with microbubbles targeted to vascular endothelial growth factor receptor type 2 in mice.

BMC medical imaging

Mancini M,Greco A,Salvatore G,Liuzzi R,Di Maro G,Vergara E,Chiappetta G,Pasquinelli R,Brunetti A,Salvatore M

13-5821 was used in Functional assays to demonstrate that CEUS with UCAVEGFR-2 might be used for in vivo non invasive detection and quantification of VEGFR-2 expression in thyroid cancer in mice, and to differentiate benign from malignant thyroid nodules.

Thu Sep 12 00:00:00 EDT 2013

ROCK suppression promotes differentiation and expansion of endothelial cells from embryonic stem cell-derived Flk1(+) mesodermal precursor cells.

Blood

Joo HJ,Choi DK,Lim JS,Park JS,Lee SH,Song S,Shin JH,Lim DS,Kim I,Hwang KC,Koh GY

13-5821 was used in Flow cytometry/Cell sorting to demonstrate a versatile method for obtaining an ample amount of ESC-derived ECs for use in therapeutic neovascularisation.

Thu Sep 27 00:00:00 EDT 2012

RUNX1 reshapes the epigenetic landscape at the onset of haematopoiesis.

The EMBO journal

Lichtinger M,Ingram R,Hannah R,Müller D,Clarke D,Assi SA,Lie-A-Ling M,Noailles L,Vijayabaskar MS,Wu M,Tenen DG,Westhead DR,Kouskoff V,Lacaud G,Göttgens B,Bonifer C

13-5821 was used in Magnetic cell separation to show that Runx1 control of haematopoietic fate is not a new regulatory layer but instead the reorganization of the epigenetic landscape.

Wed Nov 14 00:00:00 EST 2012

Systemic delivery of a breast cancer-detecting adenovirus using targeted microbubbles.

Cancer gene therapy

Warram JM,Sorace AG,Saini R,Borovjagin AV,Hoyt K,Zinn KR

13-5821 was used in Flow cytometry/Cell sorting to investigate methods to prevent inactivation of recombinant human adenovirus for the treatment of cancer gene therapy, showing the potential for systemic delivery of a breast cancer-detecting adenovirus using targeted microbubbles.

Wed Aug 01 00:00:00 EDT 2012

Etv2 is expressed in the yolk sac hematopoietic and endothelial progenitors and regulates Lmo2 gene expression.

Stem cells (Dayton, Ohio)

Koyano-Nakagawa N,Kweon J,Iacovino M,Shi X,Rasmussen TL,Borges L,Zirbes KM,Li T,Perlingeiro RC,Kyba M,Garry DJ

Published figure using CD309 (FLK1) monoclonal antibody (Product # 13-5821-82) in Flow Cytometry

Wed Aug 01 00:00:00 EDT 2012

Etv2 is expressed in the yolk sac hematopoietic and endothelial progenitors and regulates Lmo2 gene expression.

Stem cells (Dayton, Ohio)

Koyano-Nakagawa N,Kweon J,Iacovino M,Shi X,Rasmussen TL,Borges L,Zirbes KM,Li T,Perlingeiro RC,Kyba M,Garry DJ

Published figure using CD309 (FLK1) monoclonal antibody (Product # 13-5821-82) in Flow Cytometry

Wed Aug 01 00:00:00 EDT 2012

Snail promotes the cell-autonomous generation of Flk1(+) endothelial cells through the repression of the microRNA-200 family.

Stem cells and development

Gill JG,Langer EM,Lindsley RC,Cai M,Murphy TL,Murphy KM

13-5821 was used in Flow cytometry/Cell sorting to assess the role of snail to promote the cell-autonomous induction of Flk1(+) endothelial cells in mouse embryonic stem cells.

Fri Jan 20 00:00:00 EST 2012

Activation of the canonical bone morphogenetic protein (BMP) pathway during lung morphogenesis and adult lung tissue repair.

PloS one

Sountoulidis A,Stavropoulos A,Giaglis S,Apostolou E,Monteiro R,Chuva de Sousa Lopes SM,Chen H,Stripp BR,Mummery C,Andreakos E,Sideras P

Published figure using CD309 (FLK1) monoclonal antibody (Product # 13-5821-82) in Immunohistochemistry

Wed May 15 00:00:00 EDT 2013

Activation of the canonical bone morphogenetic protein (BMP) pathway during lung morphogenesis and adult lung tissue repair.

PloS one

Sountoulidis A,Stavropoulos A,Giaglis S,Apostolou E,Monteiro R,Chuva de Sousa Lopes SM,Chen H,Stripp BR,Mummery C,Andreakos E,Sideras P

Published figure using CD309 (FLK1) monoclonal antibody (Product # 13-5821-82) in Immunohistochemistry

Wed May 15 00:00:00 EDT 2013

VEGF/Flk1 signaling cascade transactivates Etv2 gene expression.

PloS one

Rasmussen TL,Shi X,Wallis A,Kweon J,Zirbes KM,Koyano-Nakagawa N,Garry DJ

Published figure using CD309 (FLK1) monoclonal antibody (Product # 13-5821-82) in Flow Cytometry

Mon May 13 00:00:00 EDT 2013

Recapitulation of the embryonic cardiovascular progenitor cell niche.

Biomaterials

Schenke-Layland K,Nsair A,Van Handel B,Angelis E,Gluck JM,Votteler M,Goldhaber JI,Mikkola HK,Kahn M,Maclellan WR

Published figure using CD309 (FLK1) monoclonal antibody (Product # 13-5821-82) in Flow Cytometry

Fri Apr 01 00:00:00 EDT 2011

Defining the earliest step of cardiovascular progenitor specification during embryonic stem cell differentiation.

The Journal of cell biology

Bondue A,Tännler S,Chiapparo G,Chabab S,Ramialison M,Paulissen C,Beck B,Harvey R,Blanpain C

13-5821 was used in Flow cytometry/Cell sorting to identify the early MCPs residing at the top of the cellular hierarchy of cardiovascular lineages during ESC differentiation.

Mon Mar 07 00:00:00 EST 2011

A triple-targeted ultrasound contrast agent provides improved localization to tumor vasculature.

Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine

Warram JM,Sorace AG,Saini R,Umphrey HR,Zinn KR,Hoyt K

13-5821 was used in Conjugation experiments to evaluate multitargeted microbubbles used for tumoural sonography, comparing single-, dual-, and triple-targeted motifs.

Fri Jul 01 00:00:00 EDT 2011

A triple-targeted ultrasound contrast agent provides improved localization to tumor vasculature.

Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine

Warram JM,Sorace AG,Saini R,Umphrey HR,Zinn KR,Hoyt K

13-5821 was used in Conjugation experiments to evaluate multitargeted microbubbles used for tumoural sonography, comparing single-, dual-, and triple-targeted motifs.

Fri Jul 01 00:00:00 EDT 2011

Major histocompatibility complex-I expression on embryonic stem cell-derived vascular progenitor cells is critical for syngeneic transplant survival.

Stem cells (Dayton, Ohio)

Ma M,Ding S,Lundqvist A,San H,Fang F,Konoplyannikov M,Berry C,Beltran LE,Chen G,Kovacic JC,Boehm M

Published figure using CD309 (FLK1) monoclonal antibody (Product # 13-5821-82) in Immunohistochemistry

Wed Sep 01 00:00:00 EDT 2010

Major histocompatibility complex-I expression on embryonic stem cell-derived vascular progenitor cells is critical for syngeneic transplant survival.

Stem cells (Dayton, Ohio)

Ma M,Ding S,Lundqvist A,San H,Fang F,Konoplyannikov M,Berry C,Beltran LE,Chen G,Kovacic JC,Boehm M

Published figure using CD309 (FLK1) monoclonal antibody (Product # 13-5821-82) in Immunohistochemistry

Wed Sep 01 00:00:00 EDT 2010

Immortalization eliminates a roadblock during cellular reprogramming into iPS cells.

Nature

Utikal J,Polo JM,Stadtfeld M,Maherali N,Kulalert W,Walsh RM,Khalil A,Rheinwald JG,Hochedlinger K

13-5821 was used in Flow cytometry/Cell sorting to investigate the rare events that allow generation of induced pluripotent stem cells.

Thu Aug 27 00:00:00 EDT 2009

Mesenchymal origin of hepatic stellate cells, submesothelial cells, and perivascular mesenchymal cells during mouse liver development.

Hepatology (Baltimore, Md.)

Asahina K,Tsai SY,Li P,Ishii M,Maxson RE,Sucov HM,Tsukamoto H

Published figure using CD309 (FLK1) monoclonal antibody (Product # 13-5821-82) in Flow Cytometry

Sun Mar 01 00:00:00 EST 2009

Mesenchymal origin of hepatic stellate cells, submesothelial cells, and perivascular mesenchymal cells during mouse liver development.

Hepatology (Baltimore, Md.)

Asahina K,Tsai SY,Li P,Ishii M,Maxson RE,Sucov HM,Tsukamoto H

Published figure using CD309 (FLK1) monoclonal antibody (Product # 13-5821-82) in Flow Cytometry

Sun Mar 01 00:00:00 EST 2009

Transcriptional activation by the Mixl1 homeodomain protein in differentiating mouse embryonic stem cells.

Stem cells (Dayton, Ohio)

Zhang H,Fraser ST,Papazoglu C,Hoatlin ME,Baron MH

13-5821 was used in Flow cytometry/Cell sorting to investigate the molecular mechanisms of Mixl1-mediated transcriptional regulation.

Tue Dec 01 00:00:00 EST 2009

US imaging of tumor angiogenesis with microbubbles targeted to vascular endothelial growth factor receptor type 2 in mice.

Radiology

Willmann JK,Paulmurugan R,Chen K,Gheysens O,Rodriguez-Porcel M,Lutz AM,Chen IY,Chen X,Gambhir SS

13-5821 was used in Immunoprecipitation to demonstrate that US imaging with microbubbles targeted to VEGFR2 allows noninvasive visualization of VEGFR2 expression in tumor vessels.

Fri Feb 01 00:00:00 EST 2008

Phosphoinositide 3-kinase signalling regulates early development and developmental haemopoiesis.

Journal of cell science

Bone HK,Welham MJ

13-5821 was used in Flow cytometry/Cell sorting to examine the involvement of phosphoinositide 3-kinase-dependent signalling during early development and haemopoiesis.

Tue May 15 00:00:00 EDT 2007

Molecular imaging of vascular endothelial growth factor receptor 2 expression using targeted contrast-enhanced high-frequency ultrasonography.

Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine

Lyshchik A,Fleischer AC,Huamani J,Hallahan DE,Brissova M,Gore JC

13-5821 was used in Immunohistochemistry to show that targeted contrast-enhanced high-frequency ultrasonography may enable in vivo molecular imaging of VEGFR2 expression on tumour vascular endothelium.

Thu Nov 01 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: CD309; CD309 antigen; Fetal liver kinase 1; FLK-1; Kinase NYK; Protein-tyrosine kinase receptor flk-1; sCD309; soluble CD309; soluble vascular endothelial growth factor receptor 2; soluble VEGFR 2; Vascular endothelial growth factor receptor 2; vascular endothelial growth factor receptor- 2; vascular endothelial growth factor receptor-2; vascular endothelial growth factor receptor-3; VEGF R2; VEGF receptor-2; VEGFR-2

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Gene Aliases: 6130401C07; Flk-1; Flk1; Kdr; Krd-1; Ly73; sVEGFR-2; VEGFR-2; VEGFR2

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UniProt ID: (Mouse) P35918

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Entrez Gene ID: (Mouse) 16542

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Function(s)

nucleotide binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity vascular endothelial growth factor-activated receptor activity integrin binding protein binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups growth factor binding vascular endothelial growth factor binding transmembrane signal receptor

Process(es)

Performance Guarantee

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CD309 (FLK1) Monoclonal Antibody (Avas12a1), Biotin, eBioscience™

CD309 (FLK1) Antibody (13-5821-82) in Flow

Product Details

13-5821-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Functional Assay (Functional)

Peptide Array (Array)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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