Invitrogen

CD335 (NKp46) Monoclonal Antibody (29A1.4), PE-Cyanine7, eBioscience™

13 Published Figures

28 References

View all (45) NKp46 (CD335) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (13)

CD335 (NKp46) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 14

CD335 (NKp46) Antibody (25-3351-82) in Flow

Staining of C57Bl/6 splenocytes with Anti-Mouse CD49b (Integrin alpha 2) APC (Product # 17-5971-82) and 0.5 µg of Rat IgG2a K Isotype Control PE-Cyanine7 (Product # 25-4321-82) (left) or 0.5 µg of Anti-Mouse CD335 (NKp46) PE-Cyanine7 (right). Total viable cells were used for analysis.

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Product Details

25-3351-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/test

View 28 publications 28 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

29A1.4

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573442

Product Specific Information

Description: The monoclonal antibody 29A1.4 recognizes mouse NKp46, also known as CD335. CD335, a member of the natural cytotoxicity receptor (NCR) family, is a glycoprotein with 2 Ig-like domains and a short cytoplasmic tail. Expression of CD335 is uniquely found on NK cells (including immature NK cells, defined as DX5- CD3-, and thereby allowing discrimination between NKT cells and NK cells (NKp46+, CD3-). Furthermore, unlike many of the NK markers which also stain NKT cells, staining with 29A1.4 is not strain specific. Staining has been shown on C57Bl/6, SJL, CBA/CA and BALB/C strains. NKp46 has been shown to play a role in NK cell-mediated lysis of several tumor cells and pathogen-infected cell lines.

The 29A1.4 monoclonal antibody has been shown to activate NK cells in vitro. The 29A1.4 monoclonal antibody does not deplete NK cells in vivo.

Applications Reported: This 29A1.4 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 29A1.4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

NKp46 (CD335, NCR1, ) is a cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. NKp46 consists of two Ig-like domains assembled to leukocyte immunoglobulin-like (LIR) and killer inhibitory receptors (KIR).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

NCR negative group 3 innate lymphoid cell (NCR^- ILC3) participates in abnormal pathology of lung in cigarette smoking-induced COPD mice.

Immunity, inflammation and disease

Chu S,Ma L,Yang X,Xiao B,Liang Y,Zheng S,Li L

25-3351-82 was used in Flow cytometry/Cell sorting to investigate the role of Natural Cytotoxicity Receptor negative innate lymphoid cells (NCR- ILC3) in the pathogenesis of chronic obstructive pulmonary disease (COPD) induced by cigarette smoking.

Wed Mar 01 00:00:00 EST 2023

RIPK3 controls MAIT cell accumulation during development but not during infection.

Cell death & disease

Patton T,Zhao Z,Lim XY,Eddy E,Wang H,Nelson AG,Ennis B,Eckle SBG,Souter MNT,Pediongco TJ,Koay HF,Zhang JG,Djajawi TM,Louis C,Lalaoui N,Jacquelot N,Lew AM,Pellicci DG,McCluskey J,Zhan Y,Chen Z,Lawlor KE,Corbett AJ

25-3351-82 was used in Flow cytometry/Cell sorting to suggest that, distinct from conventional T cells, the accumulation of MAIT cells is restrained by RIPK3 signalling, likely prior to thymic egress, in a manner independent of canonical apoptotic and necroptotic cell death pathways.

Sat Feb 11 00:00:00 EST 2023

CD169-positive macrophages enhance abscopal effect of radiofrequency ablation therapy in liver cancer.

Translational oncology

Song X,Li N,Liu Y,Wang Z,Wang T,Tan S,Li C,Qiu C,Gao L,Asano K,Tanaka M,Liang X,Liu X,Ma C

25-3351-82 was used in Flow Cytometry to establish the mouse model mimicking radiofrequency ablation (RFA)-induced abscopal effect, in which RFA eliminated the local orthotopic liver tumor but failed to control growth of distant tumor.

Sat Jan 01 00:00:00 EST 2022

SOCS2 regulation of growth hormone signaling requires a canonical interaction with phosphotyrosine.

Bioscience reports

Li K,Meza Guzman LG,Whitehead L,Leong E,Kueh A,Alexander WS,Kershaw NJ,Babon JJ,Doggett K,Nicholson SE

25-3351-82 was used in Flow cytometry/Cell sorting to indicate the critical role of phosphotyrosine binding in SOCS2 function.

Thu Dec 22 00:00:00 EST 2022

NK cells in hypoxic skin mediate a trade-off between wound healing and antibacterial defence.

Nature communications

Sobecki M,Krzywinska E,Nagarajan S,Audigé A,Hu¿nh K,Zacharjasz J,Debbache J,Kerdiles Y,Gotthardt D,Takeda N,Fandrey J,Sommer L,Sexl V,Stockmann C

Published figure using CD335 (NKp46) monoclonal antibody (Product # 25-3351-82) in Flow Cytometry

Wed Aug 04 00:00:00 EDT 2021

Interferon Lambda Regulates Cellular and Humoral Immunity in Pristane-Induced Lupus.

International journal of molecular sciences

Aschman T,Schaffer S,Biniaris Georgallis SI,Triantafyllopoulou A,Staeheli P,Voll RE

Published figure using CD335 (NKp46) monoclonal antibody (Product # 25-3351-82) in Flow Cytometry

Fri Oct 29 00:00:00 EDT 2021

Flow cytometry-based ex vivo murine NK cell cytotoxicity assay.

STAR protocols

Wong P,Wagner JA,Berrien-Elliott MM,Schappe T,Fehniger TA

25-3351-82 was used in Flow Cytometry to measure in vivo activated murine NK cells' ability to kill target cells ex vivo.

Fri Mar 19 00:00:00 EDT 2021

Flow cytometry-based ex vivo murine NK cell cytotoxicity assay.

STAR protocols

Wong P,Wagner JA,Berrien-Elliott MM,Schappe T,Fehniger TA

25-3351-82 was used in Flow Cytometry to measure in vivo activated murine NK cells' ability to kill target cells ex vivo.

Fri Mar 19 00:00:00 EDT 2021

Th2 cells promote eosinophil-independent pathology in a murine model of allergic bronchopulmonary aspergillosis.

European journal of immunology

Dietschmann A,Schruefer S,Krappmann S,Voehringer D

25-3351-82 was used in Flow Cytometry to use a mouse model of ABPA to investigate the role of eosinophils and T cell-derived IL-4/IL-13 for induction of allergic lung inflammation.

Wed Jul 01 00:00:00 EDT 2020

Characterization of the pathoimmunology of necrotizing enterocolitis reveals novel therapeutic opportunities.

Nature communications

Cho SX,Rudloff I,Lao JC,Pang MA,Goldberg R,Bui CB,McLean CA,Stock M,Klassert TE,Slevogt H,Mangan NE,Cheng W,Fischer D,Gfroerer S,Sandhu MK,Ngo D,Bujotzek A,Lariviere L,Schumacher F,Tiefenthaler G,Beker F,Collins C,Kamlin COF,König K,Malhotra A,Tan K,Theda C,Veldman A,Ellisdon AM,Whisstock JC,Berger PJ,Nold-Petry CA,Nold MF

Published figure using CD335 (NKp46) monoclonal antibody (Product # 25-3351-82) in Flow Cytometry

Fri Nov 13 00:00:00 EST 2020

Hhex Directly Represses BIM-Dependent Apoptosis to Promote NK Cell Development and Maintenance.

Cell reports

Goh W,Scheer S,Jackson JT,Hediyeh-Zadeh S,Delconte RB,Schuster IS,Andoniou CE,Rautela J,Degli-Esposti MA,Davis MJ,McCormack MP,Nutt SL,Huntington ND

25-3351 was used in Flow cytometry/Cell sorting to show homeobox transcriptional regulator Hhex regulates NK cell homeostasis and immunity.

Tue Oct 20 00:00:00 EDT 2020

Stage-Specific Requirement for Eomes in Mature NK Cell Homeostasis and Cytotoxicity.

Cell reports

Wagner JA,Wong P,Schappe T,Berrien-Elliott MM,Cubitt C,Jaeger N,Lee M,Keppel CR,Marin ND,Foltz JA,Marsala L,Neal CC,Sullivan RP,Schneider SE,Keppel MP,Saucier N,Cooper MA,Fehniger TA

25-3351 was used in Flow cytometry/Cell sorting to develop a tamoxifen-inducible, type-1-ILC-specific (Ncr1-targeted) cre mouse and combine this with Eomes-floxed mice.

Tue Jun 02 00:00:00 EDT 2020

Novel CD11b⁺Gr-1⁺Sca-1⁺ myeloid cells drive mortality in bacterial infection.

Science advances

Park MY,Kim HS,Lee HY,Zabel BA,Bae YS

Published figure using CD335 (NKp46) monoclonal antibody (Product # 25-3351-82) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2020

Cancer-Specific Loss of p53 Leads to a Modulation of Myeloid and T Cell Responses.

Cell reports

Blagih J,Zani F,Chakravarty P,Hennequart M,Pilley S,Hobor S,Hock AK,Walton JB,Morton JP,Gronroos E,Mason S,Yang M,McNeish I,Swanton C,Blyth K,Vousden KH

25-3351 was used in Flow cytometry/Cell sorting to show suppressive regulatory T cells accumulate in tumour cells in the absence of p53 indicating a pathway in which cancer cells circumvent immune destruction.

Tue Jan 14 00:00:00 EST 2020

Synergized regulation of NK cell education by NKG2A and specific Ly49 family members.

Nature communications

Zhang X,Feng J,Chen S,Yang H,Dong Z

Published figure using CD335 (NKp46) monoclonal antibody (Product # 25-3351-82) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2019

SCGN deficiency results in colitis susceptibility.

eLife

Sifuentes-Dominguez LF,Li H,Llano E,Liu Z,Singla A,Patel AS,Kathania M,Khoury A,Norris N,Rios JJ,Starokadomskyy P,Park JY,Gopal P,Liu Q,Tan S,Chan L,Ross T,Harrison S,Venuprasad K,Baker LA,Jia D,Burstein E

Published figure using CD335 (NKp46) monoclonal antibody (Product # 25-3351-82) in Flow Cytometry

Wed Oct 30 00:00:00 EDT 2019

LIF regulates CXCL9 in tumor-associated macrophages and prevents CD8⁺ T cell tumor-infiltration impairing anti-PD1 therapy.

Nature communications

Pascual-García M,Bonfill-Teixidor E,Planas-Rigol E,Rubio-Perez C,Iurlaro R,Arias A,Cuartas I,Sala-Hojman A,Escudero L,Martínez-Ricarte F,Huber-Ruano I,Nuciforo P,Pedrosa L,Marques C,Braña I,Garralda E,Vieito M,Squatrito M,Pineda E,Graus F,Espejo C,Sahuquillo J,Tabernero J,Seoane J

25-3351-82 was used in Flow Cytometry to study the effect of the combination of LIF neutralising antibodies with the inhibition of the PD1 immune checkpoint on tumour progression.

Tue Jun 11 00:00:00 EDT 2019

E3 Ligase VHL Promotes Group 2 Innate Lymphoid Cell Maturation and Function via Glycolysis Inhibition and Induction of Interleukin-33 Receptor.

Immunity

Li Q,Li D,Zhang X,Wan Q,Zhang W,Zheng M,Zou L,Elly C,Lee JH,Liu YC

25-3351 was used in Flow Cytometry to indicate that the VHL-HIF-glycolysis axis is essential for the late-stage maturation and function of ILC2s via targeting IL-33-ST2 pathway.

Tue Feb 20 00:00:00 EST 2018

Regulation of Intracellular Triiodothyronine Is Essential for Optimal Macrophage Function.

Endocrinology

van der Spek AH,Surovtseva OV,Jim KK,van Oudenaren A,Brouwer MC,Vandenbroucke-Grauls CMJE,Leenen PJM,van de Beek D,Hernandez A,Fliers E,Boelen A

25-3351 was used in Flow cytometry/Cell sorting to study the role of optimal intracellular concentrations of the active thyroid hormone triiodothyronine in proinflammatory macrophage function.

Tue May 01 00:00:00 EDT 2018

The Thyroid Hormone Inactivating Type 3 Deiodinase Is Essential for Optimal Neutrophil Function: Observations From Three Species.

Endocrinology

van der Spek AH,Jim KK,Karaczyn A,van Beeren HC,Ackermans MT,Darras VM,Vandenbroucke-Grauls CMJE,Hernandez A,Brouwer MC,Fliers E,van de Beek D,Boelen A

25-3351 was used in Flow cytometry/Cell sorting to study whether type 3 deiodinase plays a role in neutrophil function during infection.

Thu Feb 01 00:00:00 EST 2018

Conventional NK cells and ILC1 are partially ablated in the livers of Ncr1 ^iCreTbx21 ^fl/fl mice.

Wellcome open research

Cuff AO,Male V

Published figure using CD335 (NKp46) monoclonal antibody (Product # 25-3351-82) in Flow Cytometry

Sat Jan 09 00:00:00 EST 2021

Loss of HIF-1α in natural killer cells inhibits tumour growth by stimulating non-productive angiogenesis.

Nature communications

Krzywinska E,Kantari-Mimoun C,Kerdiles Y,Sobecki M,Isagawa T,Gotthardt D,Castells M,Haubold J,Millien C,Viel T,Tavitian B,Takeda N,Fandrey J,Vivier E,Sexl V,Stockmann C

Published figure using CD335 (NKp46) monoclonal antibody (Product # 25-3351-82) in Flow Cytometry

Fri Nov 17 00:00:00 EST 2017

Characterisation of innate lymphoid cell populations at different sites in mice with defective T cell immunity.

Wellcome open research

Dutton EE,Camelo A,Sleeman M,Herbst R,Carlesso G,Belz GT,Withers DR

25-3351-82 was used in Flow Cytometry to compare the innate lymphoid cell populations at different sites in mice with defective T cell immunity.

Sat Jan 09 00:00:00 EST 2021

Boosting the hypoxic response in myeloid cells accelerates resolution of fibrosis and regeneration of the liver in mice.

Oncotarget

Kantari-Mimoun C,Krzywinska E,Castells M,Milien C,Klose R,Meinecke AK,Lemberger U,Mathivet T,Gojkovic M,Schrödter K,Österreicher C,Fandrey J,Rundqvist H,Stockmann C

25-3351 was used in Flow cytometry/Cell sorting to show that the resolution of fibrosis can be accelerated by deleting VHL, a negative regulator of HIFs and VEGF, specifically in myeloid cells in mice.

Tue Feb 28 00:00:00 EST 2017

The breast tumor microenvironment alters the phenotype and function of natural killer cells.

Cellular & molecular immunology

Krneta T,Gillgrass A,Chew M,Ashkar AA

Published figure using CD335 (NKp46) monoclonal antibody (Product # 25-3351-82) in Flow Cytometry

Thu Sep 01 00:00:00 EDT 2016

NK cell development requires Tsc1-dependent negative regulation of IL-15-triggered mTORC1 activation.

Nature communications

Yang M,Chen S,Du J,He J,Wang Y,Li Z,Liu G,Peng W,Zeng X,Li D,Xu P,Guo W,Chang Z,Wang S,Tian Z,Dong Z

Published figure using CD335 (NKp46) monoclonal antibody (Product # 25-3351-82) in Flow Cytometry

Wed Sep 07 00:00:00 EDT 2016

Targeting VEGF-A in myeloid cells enhances natural killer cell responses to chemotherapy and ameliorates cachexia.

Nature communications

Klose R,Krzywinska E,Castells M,Gotthardt D,Putz EM,Kantari-Mimoun C,Chikdene N,Meinecke AK,Schrödter K,Helfrich I,Fandrey J,Sexl V,Stockmann C

25-3351 was used in Flow cytometry/Cell sorting to investigate the effect of VEGF-A genetic inactivation in myeloid cells on natural killer cell responses and chemotherapy-induced cachexia.

Fri Aug 19 00:00:00 EDT 2016

Interferon-γ from Brain Leukocytes Enhances Meningitis by Type 4 Streptococcus pneumoniae.

Frontiers in microbiology

Pettini E,Fiorino F,Cuppone AM,Iannelli F,Medaglini D,Pozzi G

25-3351 was used in Flow cytometry/Cell sorting to elucidate the role of IFN-γ in the neuroinflammatory response following type 4 Streptococcus pneumoniae-induced meningitis in mice.

Wed Dec 09 00:00:00 EST 2015

IL-22 bridges the lymphotoxin pathway with the maintenance of colonic lymphoid structures during infection with Citrobacter rodentium.

Nature immunology

Ota N,Wong K,Valdez PA,Zheng Y,Crellin NK,Diehl L,Ouyang W

Sun Aug 28 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: 1-Mar; Activating receptor 1; CD335; hNKp46; Ly-94; Lymphocyte antigen 94; mAR-1; MAR1 (mouse activating receptor 1); mNKp46; Natural cytotoxicity triggering receptor 1; Natural killer cell p46-related protein; natural killer cell receptor; NK-p46

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Gene Aliases: Ly94; Ncr1; NKp46

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UniProt ID: (Mouse) Q8C567

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Entrez Gene ID: (Mouse) 17086

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Function(s)

immunoglobulin receptor superfamily

Process(es)

detection of virus defense response to virus

Performance Guarantee

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CD335 (NKp46) Monoclonal Antibody (29A1.4), PE-Cyanine7, eBioscience™

CD335 (NKp46) Antibody (25-3351-82) in Flow

Product Details

25-3351-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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