Invitrogen

CD39 Monoclonal Antibody (24DMS1), PE-Cyanine7, eBioscience™

FIGURE: 1 / 10

CD39 Antibody (25-0391-82) in Flow

Surface staining of mouse splenocytes with Anti-Mouse CD4 FITC (Product # 11-0041-82) and 0.125 µg of Anti-Mouse CD39 PE-Cyanine7 (right), followed by intracellular staining with Anti-Mouse Foxp3 Alexa Fluor® 647. Gating for the CD4+Foxp3. cells (purple histogram) and CD4+Foxp3+ cells (blue histogram) is demonstrated on the dot plot (left).

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Product Details

25-0391-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 19 publications 19 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

24DMS1

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1210766

Product Specific Information

Description: The 24DMS1 monoclonal antibody reacts with mouse CD39, also known as NTPDaseI. E-NTPDases are enzymes that convert nucleoside tri- and diphosphates (NTDPs) into nucleoside monophosphate (NMP), thereby removing toxic extracellular ATP and ADP. CD39 is the dominant member of this family in the immune system and is involved in suppression of inflammation and control of platelet activation. CD39 can impact expression of CD73, another E-NTPase. Together, these molecules influence inflammation responses. CD39 is expressed on B cells, Langerhans cells and most monocytes. In addition, CD39 is found on a subset of CD4+ T cells that are mostly CD25+FoxP3+ T reg cells. T reg cells from CD39-null mice showed impaired suppressive properties in vitro and in vivo.

Applications Reported: This 24DMS1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 24DMS1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD39 cell surface antigen is a 70-100kD molecule expressed on peripheral blood B cells, monocytes, T cell clones, and also weakly expressed on granulocytes. CD39 has intrinsic ecto-ATPase activity. Expression of CD39 is induced on T cells and increased on B cells as a late activation antigen.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Hobit and Blimp-1 instruct the differentiation of iNKT cells into resident-phenotype lymphocytes after lineage commitment.

European journal of immunology

Kragten NA,Taggenbrock RL,Parga Vidal L,van Lier RA,Stark R,van Gisbergen KP

25-0391-82 was used in Flow cytometry/Cell sorting to conclude that memory differentiation and the establishment of residency occur after lineage commitment through a Hobit and Blimp-1-driven transcriptional program.

Tue Mar 01 00:00:00 EST 2022

Preventing type 1 diabetes in late-stage pre-diabetic NOD mice with insulin: A central role for alum as adjuvant.

Frontiers in endocrinology

Martens PJ,Ellis D,Bruggeman Y,Viaene M,Laureys J,Teyton L,Mathieu C,Gysemans C

25-0391-82 was used in Flow cytometry/Cell sorting to support the use of alum as adjuvant to optimise the efficacy of antigen-specific immunotherapy in future trials.

Tue Nov 08 00:00:00 EST 2022

Targeting the ion channel TRPM7 promotes the thymic development of regulatory T cells by promoting IL-2 signaling.

Science signaling

Mendu SK,Stremska ME,Schappe MS,Moser EK,Krupa JK,Rogers JS,Stipes EJ,Parker CA,Braciale TJ,Perry JSA,Desai BN

25-0391-82 was used in Flow Cytometry to demonstrate that inhibition of the dual ion channel and enzyme, TRPM7, increases thymic development of regulatory T cells in mice.

Tue Dec 08 00:00:00 EST 2020

A Commercial Probiotic Induces Tolerogenic and Reduces Pathogenic Responses in Experimental Autoimmune Encephalomyelitis.

Cells

Calvo-Barreiro L,Eixarch H,Ponce-Alonso M,Castillo M,Lebrón-Galán R,Mestre L,Guaza C,Clemente D,Del Campo R,Montalban X,Espejo C

25-0391 was used in Flow cytometry/Cell sorting to investigate the therapeutic effect of two commercial multispecies probiotics-Lactibiane iki and Vivomixx-on the clinical outcome of established experimental autoimmune encephalomyelitis (EAE).

Tue Apr 07 00:00:00 EDT 2020

The Inhibition of CD39 and CD73 Cell Surface Ectonucleotidases by Small Molecular Inhibitors Enhances the Mobilization of Bone Marrow Residing Stem Cells by Decreasing the Extracellular Level of Adenosine.

Stem cell reviews and reports

Adamiak M,Bujko K,Brzezniakiewicz-Janus K,Kucia M,Ratajczak J,Ratajczak MZ

25-0391 was used in Flow cytometry/Cell sorting to demonstrate mobilization of hematopoietic stem progenitor cells as well as other types of BM-residing stem cells is facilitated by CD39 and CD73 inhibitors.

Sun Dec 01 00:00:00 EST 2019

Selective deletion of ENTPD1/CD39 in macrophages exacerbates biliary fibrosis in a mouse model of sclerosing cholangitis.

Purinergic signalling

Rothweiler S,Feldbrügge L,Jiang ZG,Csizmadia E,Longhi MS,Vaid K,Enjyoji K,Popov YV,Robson SC

Published figure using CD39 monoclonal antibody (Product # 25-0391-82) in Flow Cytometry

Sun Sep 01 00:00:00 EDT 2019

Toll-Like Receptor-Mediated Activation of CD39 Internalization in BMDCs Leads to Extracellular ATP Accumulation and Facilitates P2X7 Receptor Activation.

Frontiers in immunology

Zhao R,Qiao J,Zhang X,Zhao Y,Meng X,Sun D,Peng X

Published figure using CD39 monoclonal antibody (Product # 25-0391-82) in Flow Cytometry

Thu Oct 15 00:00:00 EDT 2020

CD38-NAD⁺Axis Regulates Immunotherapeutic Anti-Tumor T Cell Response.

Cell metabolism

Chatterjee S,Daenthanasanmak A,Chakraborty P,Wyatt MW,Dhar P,Selvam SP,Fu J,Zhang J,Nguyen H,Kang I,Toth K,Al-Homrani M,Husain M,Beeson G,Ball L,Helke K,Husain S,Garrett-Mayer E,Hardiman G,Mehrotra M,Nishimura MI,Beeson CC,Bupp MG,Wu J,Ogretmen B,Paulos CM,Rathmell J,Yu XZ,Mehrotra S

25-0391 was used in Flow cytometry/Cell sorting to establish ex vivo culture conditions to generate hybrid Th1/17 cells, which persist long-term in vivo while maintaining effector function.

Tue Jan 09 00:00:00 EST 2018

CD150^high Bone Marrow Tregs Maintain Hematopoietic Stem Cell Quiescence and Immune Privilege via Adenosine.

Cell stem cell

Hirata Y,Furuhashi K,Ishii H,Li HW,Pinho S,Ding L,Robson SC,Frenette PS,Fujisaki J

Published figure using CD39 monoclonal antibody (Product # 25-0391-82) in Flow Cytometry

Thu Mar 01 00:00:00 EST 2018

Effector CD4 T cells with progenitor potential mediate chronic intestinal inflammation.

The Journal of experimental medicine

Shin B,Kress RL,Kramer PA,Darley-Usmar VM,Bellis SL,Harrington LE

25-0391 was used in Flow cytometry/Cell sorting to indicate that the chronicity of T cell-mediated inflammation is perpetuated by specific effector CD4 T cells with stem-like properties.

Mon Jul 02 00:00:00 EDT 2018

Stability and function of regulatory T cells expressing the transcription factor T-bet.

Nature

Levine AG,Mendoza A,Hemmers S,Moltedo B,Niec RE,Schizas M,Hoyos BE,Putintseva EV,Chaudhry A,Dikiy S,Fujisawa S,Chudakov DM,Treuting PM,Rudensky AY

25-0391 was used in Flow cytometry/Cell sorting to demonstrate that T-bet+ Treg cells have an essential immunosuppressive function.

Thu Jun 15 00:00:00 EDT 2017

Differentiation of human and murine induced pluripotent stem cells to microglia-like cells.

Nature neuroscience

Pandya H,Shen MJ,Ichikawa DM,Sedlock AB,Choi Y,Johnson KR,Kim G,Brown MA,Elkahloun AG,Maric D,Sweeney CL,Gossa S,Malech HL,McGavern DB,Park JK

25-0391 was used in Flow cytometry/Cell sorting to develop a technique for induced pluripotent stem cell-derived microglia-like cell differentiation.

Mon May 01 00:00:00 EDT 2017

Differentiation of human and murine induced pluripotent stem cells to microglia-like cells.

Nature neuroscience

Pandya H,Shen MJ,Ichikawa DM,Sedlock AB,Choi Y,Johnson KR,Kim G,Brown MA,Elkahloun AG,Maric D,Sweeney CL,Gossa S,Malech HL,McGavern DB,Park JK

25-0391 was used in Flow cytometry/Cell sorting to develop a technique for induced pluripotent stem cell-derived microglia-like cell differentiation.

Mon May 01 00:00:00 EDT 2017

BTK inhibition results in impaired CXCR4 chemokine receptor surface expression, signaling and function in chronic lymphocytic leukemia.

Leukemia

Chen SS,Chang BY,Chang S,Tong T,Ham S,Sherry B,Burger JA,Rai KR,Chiorazzi N

25-0391 was used in Flow cytometry/Cell sorting to identify CXCR4 as a key regulator in BTK-mediated CLL-cell retention.

Fri Apr 01 00:00:00 EDT 2016

Immunosuppression-Independent Role of Regulatory T Cells against Hypertension-Driven Renal Dysfunctions.

Molecular and cellular biology

Fabbiano S,Menacho-Márquez M,Robles-Valero J,Pericacho M,Matesanz-Marín A,García-Macías C,Sevilla MA,Montero MJ,Alarcón B,López-Novoa JM,Martín P,Bustelo XR

25-0391 was used in Flow cytometry/Cell sorting to determine that TREG cells constitute a first protective barrier against hypertension-driven tissue fibrosis.

Thu Oct 01 00:00:00 EDT 2015

Purinergic signaling on leukocytes infiltrating the LPS-injured lung.

PloS one

Friebe D,Yang T,Schmidt T,Borg N,Steckel B,Ding Z,Schrader J

25-0391 was used in Flow cytometry/Cell sorting to investigate the role of extracellular nucleotides/nucleosides in acute lung injury, showing purinergic signaling on leukocytes infiltrating the injured lung.

Thu Jan 15 00:00:00 EST 2015

Ecto-5'-nucleotidase on immune cells protects from adverse cardiac remodeling.

Circulation research

Bönner F,Borg N,Jacoby C,Temme S,Ding Z,Flögel U,Schrader J

25-0391 was used in Flow cytometry/Cell sorting to examine the role of CD73 in cardiac repair and remodelling in mice after reperfused myocardial infarction.

Fri Jul 19 00:00:00 EDT 2013

IL-17A and IL-2-expanded regulatory T cells cooperate to inhibit Th1-mediated rejection of MHC II disparate skin grafts.

PloS one

Vokaer B,Charbonnier LM,Lemaître PH,Spilleboudt C,Le Moine A

25-0391 was used in Flow cytometry/Cell sorting to evaluate the effect of IL-17A neutralisation in a mouse model of skin transplantation.

Thu Jul 24 00:00:00 EDT 2014

Resident cardiac immune cells and expression of the ectonucleotidase enzymes CD39 and CD73 after ischemic injury.

PloS one

Bönner F,Borg N,Burghoff S,Schrader J

25-0391 was used in Flow cytometry/Cell sorting to explore the abundance of CD73 and CD39 on immune cells under control conditions and in response to inflammation.

Thu Aug 30 00:00:00 EDT 2012

Self-regulation of inflammatory cell trafficking in mice by the leukocyte surface apyrase CD39.

The Journal of clinical investigation

Hyman MC,Petrovic-Djergovic D,Visovatti SH,Liao H,Yanamadala S,Bouïs D,Su EJ,Lawrence DA,Broekman MJ,Marcus AJ,Pinsky DJ

Fri May 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: ATP diphosphohydrolase; ATP-DPH; CD39; DKFZp686D194; DKFZp686I093; Ecto-apyrase; Ecto-ATP diphosphohydrolase 1; Ecto-ATPDase 1; Ectonucleoside triphosphate diphosphohydrolase 1; FLJ40921; FLJ40959; Lymphoid cell activation antigen; NTPDase 1; NTPDase1; Nucleoside triphosphate diphosphohydrolase 1

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Gene Aliases: 2610206B08Rik; AA408691; Cd39; Entpd1; NTPDase-1

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UniProt ID: (Mouse) P55772

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Entrez Gene ID: (Mouse) 12495

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Function(s)

hydrolase nucleotide phosphatase phosphatase metabolite interconversion enzyme

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD39 Monoclonal Antibody (24DMS1), PE-Cyanine7, eBioscience™

CD39 Antibody (25-0391-82) in Flow

Product Details

25-0391-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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