Invitrogen

CD39 Monoclonal Antibody (eBioA1 (A1)), FITC, eBioscience™

FIGURE: 1 / 15

CD39 Antibody (11-0399-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD19 APC (Product # 17-0199-42) and Mouse IgG1 kappa Isotype Control FITC (Product # 11-4714-42) (left) or Anti-Human CD39 FITC (right). Cells in the lymphocyte gate were used for analysis.

CD39 Antibody in Immunohistochemistry (IHC)

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Product Details

11-0399-42

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 18 publications 18 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioA1 (A1)

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11151149

Product Specific Information

Description: The eBioA1 monoclonal antibody reacts with human CD39 also known as ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1) or NTPDase. CD39 is an integral membrane protein with two transmembrane domains and exists as a homotetramer. It is the most prominent ectoenzyme of the immune system. The function of CD39 is to effectively remove toxic extracellular ATP by converting it to ADP or AMP. CD39 is thought to work together with CD73 to hydrolyze ATP and has been well characterized on Langerhans cells. Expression of CD39 was originally identified on activated lymphocytes. Expression is also found on a subset of T cells, B cells and dendritic cells as well as weak staining on monocytes and granulocytes.

Recently, CD39 and CD73 have been found on regulatory T cells (Treg). Expression of CD39 on Treg may facilitate their entry into inflamed areas where high levels of ATP are present. Expression of CD39 on Foxp3+CD4+ cells ranges from 25-45%.

Applications Reported: This eBioA1 (A1) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioA1 (A1) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD39 cell surface antigen is a 70-100kD molecule expressed on peripheral blood B cells, monocytes, T cell clones, and also weakly expressed on granulocytes. CD39 has intrinsic ecto-ATPase activity. Expression of CD39 is induced on T cells and increased on B cells as a late activation antigen.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Single-cell analysis of immune cells on gingiva-derived mesenchymal stem cells in experimental autoimmune uveitis.

iScience

Gao Y,Duan R,Li H,Jiang L,Tao T,Liu X,Zhu L,Li Z,Chen B,Zheng S,Lin X,Su W

11-0399-42 was used in Flow cytometry/Cell sorting to generate a lymph node single-cell transcriptomic atlas of GMSC-treated experimental autoimmune uveitis mice.

Fri May 19 00:00:00 EDT 2023

Pinpointing the tumor-specific T cells via TCR clusters.

eLife

Goncharov MM,Bryushkova EA,Sharaev NI,Skatova VD,Baryshnikova AM,Sharonov GV,Karnaukhov V,Vakhitova MT,Samoylenko IV,Demidov LV,Lukyanov S,Chudakov DM,Serebrovskaya EO

Published figure using CD39 monoclonal antibody (Product # 11-0399-42) in Flow Cytometry

Mon Apr 04 00:00:00 EDT 2022

Impact of Early ARV Initiation on Relative Proportions of Effector and Regulatory CD8 T Cell in Mesenteric Lymph Nodes and Peripheral Blood During Acute SIV Infection of Rhesus Macaques.

Journal of virology

Yero A,Farnos O,Clain J,Zghidi-Abouzid O,Rabezanahary H,Racine G,Estaquier J,Jenabian MA

11-0399-42 was used in Flow cytometry/Cell sorting to provide deeper insight into the dynamics of the CD8 T-cell compartment in gut mucosal immune surveillance during acute SIV infection and following early ARV initiation.

Wed Apr 13 00:00:00 EDT 2022

Neoadjuvant anti-OX40 (MEDI6469) therapy in patients with head and neck squamous cell carcinoma activates and expands antigen-specific tumor-infiltrating T cells.

Nature communications

Duhen R,Ballesteros-Merino C,Frye AK,Tran E,Rajamanickam V,Chang SC,Koguchi Y,Bifulco CB,Bernard B,Leidner RS,Curti BD,Fox BA,Urba WJ,Bell RB,Weinberg AD

Published figure using CD39 monoclonal antibody (Product # 11-0399-42) in Flow Cytometry

Tue Feb 16 00:00:00 EST 2021

Molecular profiling of driver events in metastatic uveal melanoma.

Nature communications

Karlsson J,Nilsson LM,Mitra S,Alsén S,Shelke GV,Sah VR,Forsberg EMV,Stierner U,All-Eriksson C,Einarsdottir B,Jespersen H,Ny L,Lindnér P,Larsson E,Olofsson Bagge R,Nilsson JA

Published figure using CD39 monoclonal antibody (Product # 11-0399-42) in Flow Cytometry

Mon Apr 20 00:00:00 EDT 2020

Immune Suppressive Effects of Plasma-Derived Exosome Populations in Head and Neck Cancer.

Cancers

Beccard IJ,Hofmann L,Schroeder JC,Ludwig S,Laban S,Brunner C,Lotfi R,Hoffmann TK,Jackson EK,Schuler PJ,Theodoraki MN

Published figure using CD39 monoclonal antibody (Product # 11-0399-42) in Flow Cytometry

Tue Jul 21 00:00:00 EDT 2020

Circulating Exosomes Inhibit B Cell Proliferation and Activity.

Cancers

Schroeder JC,Puntigam L,Hofmann L,Jeske SS,Beccard IJ,Doescher J,Laban S,Hoffmann TK,Brunner C,Theodoraki MN,Schuler PJ

Published figure using CD39 monoclonal antibody (Product # 11-0399-42) in Flow Cytometry

Wed Jul 29 00:00:00 EDT 2020

Differential Dynamics of Regulatory T-Cell and Th17 Cell Balance in Mesenteric Lymph Nodes and Blood following Early Antiretroviral Initiation during Acute Simian Immunodeficiency Virus Infection.

Journal of virology

Yero A,Farnos O,Rabezanahary H,Racine G,Estaquier J,Jenabian MA

Published figure using CD39 monoclonal antibody (Product # 11-0399-42) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2019

Spatial and Single-Cell Transcriptional Profiling Identifies Functionally Distinct Human Dermal Fibroblast Subpopulations.

The Journal of investigative dermatology

Philippeos C,Telerman SB,Oulès B,Pisco AO,Shaw TJ,Elgueta R,Lombardi G,Driskell RR,Soldin M,Lynch MD,Watt FM

Published figure using CD39 monoclonal antibody (Product # 11-0399-42) in Immunofluorescence

Sun Apr 01 00:00:00 EDT 2018

Spatial and Single-Cell Transcriptional Profiling Identifies Functionally Distinct Human Dermal Fibroblast Subpopulations.

The Journal of investigative dermatology

Philippeos C,Telerman SB,Oulès B,Pisco AO,Shaw TJ,Elgueta R,Lombardi G,Driskell RR,Soldin M,Lynch MD,Watt FM

Published figure using CD39 monoclonal antibody (Product # 11-0399-42) in Immunofluorescence

Sun Apr 01 00:00:00 EDT 2018

The influence of chemotherapy on adenosine-producing B cells in patients with head and neck squamous cell carcinoma.

Oncotarget

Ziebart A,Huber U,Jeske S,Laban S,Doescher J,Hoffmann TK,Brunner C,Jackson EK,Schuler PJ

Published figure using CD39 monoclonal antibody (Product # 11-0399-42) in Flow Cytometry

Fri Jan 19 00:00:00 EST 2018

Functionalization-dependent effects of cellulose nanofibrils on tolerogenic mechanisms of human dendritic cells.

International journal of nanomedicine

Tomić S,Ilić N,Kokol V,Gruden-Movsesijan A,Mihajlović D,Bekić M,Sofronić-Milosavljević L,Čolić M,Vučević D

11-0399 was used in Flow cytometry/Cell sorting to investigate how differentially functionalised cellulose nanofibrils affect dendritic cell properties, their viability, morphology, differentiation and maturation potential, and the capacity to regulate T cell-mediated immune response.

Mon Dec 31 00:00:00 EST 2018

Impact of immunosuppressive drugs on the therapeutic efficacy of ex vivo expanded human regulatory T cells.

Haematologica

Scottà C,Fanelli G,Hoong SJ,Romano M,Lamperti EN,Sukthankar M,Guggino G,Fazekasova H,Ratnasothy K,Becker PD,Afzali B,Lechler RI,Lombardi G

11-0399 was used in Flow cytometry/Cell sorting to study the effect of immunosuppressive drugs on Treg cells.

Fri Jan 01 00:00:00 EST 2016

Tumor-derived exosomes regulate expression of immune function-related genes in human T cell subsets.

Scientific reports

Muller L,Mitsuhashi M,Simms P,Gooding WE,Whiteside TL

Published figure using CD39 monoclonal antibody (Product # 11-0399-42) in Flow Cytometry

Thu Feb 04 00:00:00 EST 2016

Bone marrow-derived mesenchymal stromal cells harness purinergenic signaling to tolerize human Th1 cells in vivo.

Stem cells (Dayton, Ohio)

Amarnath S,Foley JE,Farthing DE,Gress RE,Laurence A,Eckhaus MA,Métais JY,Rose JJ,Hakim FT,Felizardo TC,Cheng AV,Robey PG,Stroncek DE,Sabatino M,Battiwalla M,Ito S,Fowler DH,Barrett AJ

11-0399 was used in Flow cytometry/Cell sorting to investigate the mechanism of BMSC in treatment of alloimmune and autoimmune conditions, showing that they harness purinergenic signaling to tolerize human Th1 cells.

Wed Apr 01 00:00:00 EDT 2015

Tumor-derived exosomes promote tumor progression and T-cell dysfunction through the regulation of enriched exosomal microRNAs in human nasopharyngeal carcinoma.

Oncotarget

Ye SB,Li ZL,Luo DH,Huang BJ,Chen YS,Zhang XS,Cui J,Zeng YX,Li J

11-0399 was used in Flow cytometry/Cell sorting to show that tumour-derived exosomes promote tumour progression and T-cell dysfunction through regulation of exosomal microRNAs in human carcinomas.

Wed Jul 30 00:00:00 EDT 2014

Mesenchymal stem cells from periapical lesions modulate differentiation and functional properties of monocyte-derived dendritic cells.

European journal of immunology

Dokić J,Tomić S,Marković M,Milosavljević P,Colić M

11-0399 was used in Flow cytometry/Cell sorting to investigate whether mesenchymal stem cells from periapical lesions modulate the development and functions of dendritic cells.

Mon Jul 01 00:00:00 EDT 2013

OMIP-006: phenotypic subset analysis of human T regulatory cells via polychromatic flow cytometry.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Murdoch DM,Staats JS,Weinhold KJ

Published figure using CD39 monoclonal antibody (Product # 11-0399-42) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2012

Frequency of circulating regulatory T cells increases during chronic HIV infection and is largely controlled by highly active antiretroviral therapy.

PloS one

Presicce P,Orsborn K,King E,Pratt J,Fichtenbaum CJ,Chougnet CA

11-0399 was used in Flow cytometry/Cell sorting to analyse Treg frequency and phenotype in 11 HIV-infected individuals enrolled in a single, prospective clinical trial.

Fri Jul 20 00:00:00 EDT 2012

Immunohistochemical markers for quantitative studies of neurons and glia in human neocortex.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Lyck L,Dalmau I,Chemnitz J,Finsen B,Schrøder HD

Sat Mar 01 00:00:00 EST 2008

Bioinformatics

Protein Aliases: ATP diphosphohydrolase; ATP-DPH; CD39; CD39 antigen; DKFZp686D194; DKFZp686I093; Ecto-apyrase; Ecto-ATP diphosphohydrolase 1; ecto-ATPase 1; Ecto-ATPDase 1; Ectonucleoside triphosphate diphosphohydrolase 1; FLJ40921; FLJ40959; Lymphoid cell activation antigen; NTPDase1; Nucleoside triphosphate diphosphohydrolase 1

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Gene Aliases: ATPDase; CD39; ENTPD1; NTPDase-1; SPG64

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UniProt ID: (Human) P49961

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Entrez Gene ID: (Human) 953

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Function(s)

protein binding ATP binding hydrolase activity hydrolase nucleotide phosphatase phosphatase metabolite interconversion enzyme

Process(es)

cell adhesion blood coagulation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD39 Monoclonal Antibody (eBioA1 (A1)), FITC, eBioscience™

CD39 Antibody (11-0399-42) in Flow

Product Details

11-0399-42

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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