Invitrogen

CD39 Monoclonal Antibody (eBioA1 (A1)), eBioscience™

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CD39 Antibody (14-0399-82) in Flow

Staining of normal human peripheral blood cells with 0.125 µg of Mouse IgG1 kappa Isotype Control Purified (Product # 14-4714-82) (open histogram) or Anti-Human CD39 Purified (filled histogram) followed by Anti-Mouse IgG FITC (Product # 11-4011-85). Cells in the lymphocyte gate were used for analysis.

CD39 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

14-0399-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.25 µg/test

View 14 publications 14 publications

ELISA (ELISA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

eBioA1 (A1)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_837126

Product Specific Information

Description: The eBioA1 monoclonal antibody reacts with human CD39 also known as ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1) or NTPDase. CD39 is an integral membrane protein with two transmembrane domains and exists as a homotetramer. It is the most prominent ectoenzyme of the immune system. The function of CD39 is to effectively remove toxic extracellular ATP by converting it to ADP or AMP. CD39 is thought to work together with CD73 to hydrolyze ATP and has been well characterized on Langerhans cells. Expression of CD39 was originally identified on activated lymphocytes. Expression is also found on a subset of T cells, B cells and dendritic cells as well as weak staining on monocytes and granulocytes.

Recently, CD39 and CD73 have been found on regulatory T cells (Treg). Expression of CD39 on Treg may facilitate their entry into inflamed areas where high levels of ATP are present. Expression of CD39 on Foxp3+CD4+ cells ranges from 25-45%.

Applications Reported: This eBioA1 (A1) antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining.

Applications Tested: This eBioA1 (A1) antibody has been tested by flow cytometric analysis of nomral human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD39 cell surface antigen is a 70-100kD molecule expressed on peripheral blood B cells, monocytes, T cell clones, and also weakly expressed on granulocytes. CD39 has intrinsic ecto-ATPase activity. Expression of CD39 is induced on T cells and increased on B cells as a late activation antigen.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Pinpointing the tumor-specific T cells via TCR clusters.

eLife

Goncharov MM,Bryushkova EA,Sharaev NI,Skatova VD,Baryshnikova AM,Sharonov GV,Karnaukhov V,Vakhitova MT,Samoylenko IV,Demidov LV,Lukyanov S,Chudakov DM,Serebrovskaya EO

Published figure using CD39 monoclonal antibody (Product # 14-0399-82) in Flow Cytometry

Mon Apr 04 00:00:00 EDT 2022

Neoadjuvant anti-OX40 (MEDI6469) therapy in patients with head and neck squamous cell carcinoma activates and expands antigen-specific tumor-infiltrating T cells.

Nature communications

Duhen R,Ballesteros-Merino C,Frye AK,Tran E,Rajamanickam V,Chang SC,Koguchi Y,Bifulco CB,Bernard B,Leidner RS,Curti BD,Fox BA,Urba WJ,Bell RB,Weinberg AD

Published figure using CD39 monoclonal antibody (Product # 14-0399-82) in Flow Cytometry

Tue Feb 16 00:00:00 EST 2021

Fully human anti-CD39 antibody potently inhibits ATPase activity in cancer cells via uncompetitive allosteric mechanism.

mAbs

Spatola BN,Lerner AG,Wong C,Dela Cruz T,Welch M,Fung W,Kovalenko M,Losenkova K,Yegutkin GG,Beers C,Corbin J,Soros VB

14-0399-82 was used in ELISA to identify TTX-030, a human anti-CD39 antibody, as a potent allosteric inhibitor of CD39 ATPase activity in cancer cells.

Mon Sep 20 00:00:00 EDT 2021

Molecular profiling of driver events in metastatic uveal melanoma.

Nature communications

Karlsson J,Nilsson LM,Mitra S,Alsén S,Shelke GV,Sah VR,Forsberg EMV,Stierner U,All-Eriksson C,Einarsdottir B,Jespersen H,Ny L,Lindnér P,Larsson E,Olofsson Bagge R,Nilsson JA

Published figure using CD39 monoclonal antibody (Product # 14-0399-82) in Flow Cytometry

Mon Apr 20 00:00:00 EDT 2020

Immune Suppressive Effects of Plasma-Derived Exosome Populations in Head and Neck Cancer.

Cancers

Beccard IJ,Hofmann L,Schroeder JC,Ludwig S,Laban S,Brunner C,Lotfi R,Hoffmann TK,Jackson EK,Schuler PJ,Theodoraki MN

Published figure using CD39 monoclonal antibody (Product # 14-0399-82) in Flow Cytometry

Tue Jul 21 00:00:00 EDT 2020

Circulating Exosomes Inhibit B Cell Proliferation and Activity.

Cancers

Schroeder JC,Puntigam L,Hofmann L,Jeske SS,Beccard IJ,Doescher J,Laban S,Hoffmann TK,Brunner C,Theodoraki MN,Schuler PJ

Published figure using CD39 monoclonal antibody (Product # 14-0399-82) in Flow Cytometry

Wed Jul 29 00:00:00 EDT 2020

Differential Dynamics of Regulatory T-Cell and Th17 Cell Balance in Mesenteric Lymph Nodes and Blood following Early Antiretroviral Initiation during Acute Simian Immunodeficiency Virus Infection.

Journal of virology

Yero A,Farnos O,Rabezanahary H,Racine G,Estaquier J,Jenabian MA

Published figure using CD39 monoclonal antibody (Product # 14-0399-82) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2019

HPV-16 Infection Is Associated with a High Content of CD39 and CD73 Ectonucleotidases in Cervical Samples from Patients with CIN-1.

Mediators of inflammation

de Lourdes Mora-García M,López-Cisneros S,Gutiérrez-Serrano V,García-Rocha R,Weiss-Steider B,Hernández-Montes J,Sánchez-Peña HI,Ávila-Ibarra LR,Don-López CA,Muñóz-Godínez R,Pineda DBT,Chacón-Salinas R,Vallejo-Castillo L,Pérez-Tapia SM,Monroy-García A

14-0399-82 was used in Immunocytochemistry to generate an immunosuppressive microenvironment and allow its progression to CeCa.

Mon Jan 13 00:00:00 EST 2020

Spatial and Single-Cell Transcriptional Profiling Identifies Functionally Distinct Human Dermal Fibroblast Subpopulations.

The Journal of investigative dermatology

Philippeos C,Telerman SB,Oulès B,Pisco AO,Shaw TJ,Elgueta R,Lombardi G,Driskell RR,Soldin M,Lynch MD,Watt FM

Sun Apr 01 00:00:00 EDT 2018

Spatial and Single-Cell Transcriptional Profiling Identifies Functionally Distinct Human Dermal Fibroblast Subpopulations.

The Journal of investigative dermatology

Philippeos C,Telerman SB,Oulès B,Pisco AO,Shaw TJ,Elgueta R,Lombardi G,Driskell RR,Soldin M,Lynch MD,Watt FM

Sun Apr 01 00:00:00 EDT 2018

The influence of chemotherapy on adenosine-producing B cells in patients with head and neck squamous cell carcinoma.

Oncotarget

Ziebart A,Huber U,Jeske S,Laban S,Doescher J,Hoffmann TK,Brunner C,Jackson EK,Schuler PJ

Published figure using CD39 monoclonal antibody (Product # 14-0399-82) in Flow Cytometry

Fri Jan 19 00:00:00 EST 2018

Expression of a Chimeric Antigen Receptor Specific for Donor HLA Class I Enhances the Potency of Human Regulatory T Cells in Preventing Human Skin Transplant Rejection.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Boardman DA,Philippeos C,Fruhwirth GO,Ibrahim MA,Hannen RF,Cooper D,Marelli-Berg FM,Watt FM,Lechler RI,Maher J,Smyth LA,Lombardi G

14039982 was used in flow cytometry to observe that the use of chimeric antigen receptor technology is a clinically applicable refinement of regulatory T cell therapy for organ transplantation

Sat Apr 01 00:00:00 EDT 2017

Tumor-derived exosomes regulate expression of immune function-related genes in human T cell subsets.

Scientific reports

Muller L,Mitsuhashi M,Simms P,Gooding WE,Whiteside TL

Published figure using CD39 monoclonal antibody (Product # 14-0399-82) in Flow Cytometry

Thu Feb 04 00:00:00 EST 2016

Inhibition of CD39 enzymatic function at the surface of tumor cells alleviates their immunosuppressive activity.

Cancer immunology research

Bastid J,Regairaz A,Bonnefoy N,Déjou C,Giustiniani J,Laheurte C,Cochaud S,Laprevotte E,Funck-Brentano E,Hemon P,Gros L,Bec N,Larroque C,Alberici G,Bensussan A,Eliaou JF

14-0399 was used in Flow cytometry/Cell sorting to address whether CD39 is expressed by tumour cells and whether CD39+ tumour cells mediate immunosuppression via the adenosine pathway.

Sun Mar 01 00:00:00 EST 2015

Enhanced production of IL-17A in patients with severe asthma is inhibited by 1α,25-dihydroxyvitamin D3 in a glucocorticoid-independent fashion.

The Journal of allergy and clinical immunology

Nanzer AM,Chambers ES,Ryanna K,Richards DF,Black C,Timms PM,Martineau AR,Griffiths CJ,Corrigan CJ,Hawrylowicz CM

14-0399 was used in Flow cytometry/Cell sorting to investigate the production of the Th17-associated cytokines IL-17A and IL-22 by peripheral blood mononuclear cells from patients with moderate-to-severe asthma.

Thu Aug 01 00:00:00 EDT 2013

Helios+ and Helios- cells coexist within the natural FOXP3+ T regulatory cell subset in humans.

Journal of immunology (Baltimore, Md. : 1950)

Himmel ME,MacDonald KG,Garcia RV,Steiner TS,Levings MK

14-0399 was used in Flow cytometry/Cell sorting to investigate the link between Helios expression and natural regulatory FOXP3+ T cell subsets from different origins in humans.

Fri Mar 01 00:00:00 EST 2013

OMIP-006: phenotypic subset analysis of human T regulatory cells via polychromatic flow cytometry.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Murdoch DM,Staats JS,Weinhold KJ

Published figure using CD39 monoclonal antibody (Product # 14-0399-82) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2012

Immunohistochemical markers for quantitative studies of neurons and glia in human neocortex.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Lyck L,Dalmau I,Chemnitz J,Finsen B,Schrøder HD

Sat Mar 01 00:00:00 EST 2008

Bioinformatics

Protein Aliases: ATP diphosphohydrolase; ATP-DPH; CD39; CD39 antigen; DKFZp686D194; DKFZp686I093; Ecto-apyrase; Ecto-ATP diphosphohydrolase 1; ecto-ATPase 1; Ecto-ATPDase 1; Ectonucleoside triphosphate diphosphohydrolase 1; FLJ40921; FLJ40959; Lymphoid cell activation antigen; NTPDase1; Nucleoside triphosphate diphosphohydrolase 1

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Gene Aliases: ATPDase; CD39; ENTPD1; NTPDase-1; SPG64

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UniProt ID: (Human) P49961

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Entrez Gene ID: (Human) 953

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Function(s)

protein binding ATP binding hydrolase activity hydrolase nucleotide phosphatase phosphatase metabolite interconversion enzyme

Process(es)

cell adhesion blood coagulation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD39 Monoclonal Antibody (eBioA1 (A1)), eBioscience™

CD39 Antibody (14-0399-82) in Flow

Product Details

14-0399-82

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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