Invitrogen

CD40 Monoclonal Antibody (1C10), Super Bright™ 436, eBioscience™

12 Published Figures

14 References

View all (194) CD40 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (12)

FIGURE: 1 / 13

CD40 Antibody (62-0401-82) in Flow

Staining of BALB/c splenocytes with Anti-Mouse CD19 FITC (Product # 11-0193) and 0.25 µg of Rat IgG2a K Isotype Control Super Bright 436 (Product # 62-4321) (left) or 0.25 µg of Anti-Mouse CD40 Super Bright 436 (Product # 62-0401) (right). Total viable cells were used for analysis.

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Product Details

62-0401-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 14 publications 14 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 436, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

1C10

Conjugate

Super Bright™ 436 Super Bright™ 436 Super Bright™ 436

Excitation/Emission Max

413/431 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2716991

Product Specific Information

Description: The 1C10 monoclonal antibody reacts with mouse CD40, a 45-50 kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by mouse B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development/maturation by inducing Ig isotype switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39), its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation.

The monoclonal antibody 1C10 is reported to have agonistic activity in vitro and in vivo.

Applications Reported: The 1C10 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 1C10 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

The CD40 antigen is a single chain glycoprotein that is known to be a member of the tumor necrosis factor/nerve growth factor superfamily and shows a significant homology to the Hodgkin's disease associated antigen, CD30. CD40 is present on all B cells except plasma cells and is also found on some epithelial cells, carcinomas and lymphoid dendritic cells. CD40 has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of CD40, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with CD40 and serves as a mediator of the signal transduction. The interaction of CD40 and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Two alternatively spliced transcript variants of CD40 encoding distinct isoforms have been reported. Diseases associated with CD40 dysfunction include Type 3 Hyper-Igm immunodeficiency and CD40 ligand deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Fasudil-modified macrophages reduce inflammation and regulate the immune response in experimental autoimmune encephalomyelitis.

Neural regeneration research

Liu C,Guo S,Liu R,Guo M,Wang Q,Chai Z,Xiao B,Ma C

Published figure using CD40 monoclonal antibody (Product # 62-0401-82) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2024

Prostaglandin E2 serves a dual role in regulating the migration of dendritic cells.

International journal of molecular medicine

Diao G,Huang J,Zheng X,Sun X,Tian M,Han J,Guo J

Published figure using CD40 monoclonal antibody (Product # 62-0401-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Myeloid and plasmacytoid dendritic cell combined vaccines loaded with heat-treated tumor cell lysates enhance antitumor activity in murine lung cancer.

Oncology letters

Chen H,Tan J,Li X,Li H,Wu W,Wu Y,Zhang J,Gu L

Published figure using CD40 monoclonal antibody (Product # 62-0401-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Neuroimmune Consequences of eIF4E Phosphorylation on Chemotherapy-Induced Peripheral Neuropathy.

Frontiers in immunology

Agalave NM,Mody PH,Szabo-Pardi TA,Jeong HS,Burton MD

Published figure using CD40 monoclonal antibody (Product # 62-0401-82) in Flow Cytometry

Mon Sep 20 00:00:00 EDT 2021

Modulation of lactate-lysosome axis in dendritic cells by clotrimazole potentiates antitumor immunity.

Journal for immunotherapy of cancer

Wang Z,Xu F,Hu J,Zhang H,Cui L,Lu W,He W,Wang X,Li M,Zhang H,Xiong W,Xie C,Liu Y,Zhou P,Liu J,Huang P,Qin XF,Xia X

Published figure using CD40 monoclonal antibody (Product # 62-0401-82) in Flow Cytometry

Sat May 01 00:00:00 EDT 2021

E6‑regulated overproduction of prostaglandin E2 may inhibit migration of dendritic cells in human papillomavirus 16‑positive cervical lesions.

International journal of oncology

Huang J,Diao G,Zhang Q,Chen Y,Han J,Guo J

Published figure using CD40 monoclonal antibody (Product # 62-0401-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

Senolytics prevent mt-DNA-induced inflammation and promote the survival of aged organs following transplantation.

Nature communications

Iske J,Seyda M,Heinbokel T,Maenosono R,Minami K,Nian Y,Quante M,Falk CS,Azuma H,Martin F,Passos JF,Niemann CU,Tchkonia T,Kirkland JL,Elkhal A,Tullius SG

Published figure using CD40 monoclonal antibody (Product # 62-0401-82) in Flow Cytometry

Thu Aug 27 00:00:00 EDT 2020

Tregitopes regulate the tolerogenic immune response and decrease the foetal death rate in abortion-prone mouse matings.

Scientific reports

Kedzierska AE,Lorek D,Slawek A,Chelmonska-Soyta A

Published figure using CD40 monoclonal antibody (Product # 62-0401-82) in Flow Cytometry

Mon Jun 29 00:00:00 EDT 2020

Polyanhydride Nanoparticles Induce Low Inflammatory Dendritic Cell Activation Resulting in CD8⁺ T Cell Memory and Delayed Tumor Progression.

International journal of nanomedicine

Darling R,Senapati S,Christiansen J,Liu L,Ramer-Tait AE,Narasimhan B,Wannemuehler M

Published figure using CD40 monoclonal antibody (Product # 62-0401-82) in Flow Cytometry

Mon Dec 07 00:00:00 EST 2020

cGAS/STING axis mediates a topoisomerase II inhibitor-induced tumor immunogenicity.

The Journal of clinical investigation

Wang Z,Chen J,Hu J,Zhang H,Xu F,He W,Wang X,Li M,Lu W,Zeng G,Zhou P,Huang P,Chen S,Li W,Xia LP,Xia X

Published figure using CD40 monoclonal antibody (Product # 62-0401-82) in Flow Cytometry

Tue Aug 13 00:00:00 EDT 2019

Aggregated and Hyperstable Damage-Associated Molecular Patterns Are Released During ER Stress to Modulate Immune Function.

Frontiers in cell and developmental biology

Andersohn A,Garcia MI,Fan Y,Thompson MC,Akimzhanov AM,Abdullahi A,Jeschke MG,Boehning D

62-0401-82 was used in Flow Cytometry to show that induction of chronic ER stress leads to the release of known and novel damage-associated molecular patterns.

Mon Sep 28 00:00:00 EDT 2020

Efficient oral vaccination by bioengineering virus-like particles with protozoan surface proteins.

Nature communications

Serradell MC,Rupil LL,Martino RA,Prucca CG,Carranza PG,Saura A,Fernández EA,Gargantini PR,Tenaglia AH,Petiti JP,Tonelli RR,Reinoso-Vizcaino N,Echenique J,Berod L,Piaggio E,Bellier B,Sparwasser T,Klatzmann D,Luján HD

Published figure using CD40 monoclonal antibody (Product # 62-0401-82) in Flow Cytometry

Mon Jan 21 00:00:00 EST 2019

Paeoniflorin Ameliorates Experimental Autoimmune Encephalomyelitis via Inhibition of Dendritic Cell Function and Th17 Cell Differentiation.

Scientific reports

Zhang H,Qi Y,Yuan Y,Cai L,Xu H,Zhang L,Su B,Nie H

Published figure using CD40 monoclonal antibody (Product # 62-0401-82) in Flow Cytometry

Mon Feb 06 00:00:00 EST 2017

Tumor-derived exosomes educate dendritic cells to promote tumor metastasis via HSP72/HSP105-TLR2/TLR4 pathway.

Oncoimmunology

Shen Y,Guo D,Weng L,Wang S,Ma Z,Yang Y,Wang P,Wang J,Cai Z

Published figure using CD40 monoclonal antibody (Product # 62-0401-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Bioinformatics

Protein Aliases: B-cell surface antigen CD40; Bp50; CD antigen CD40; CD40; CD40L receptor; Immunoglobulin M; ImmunoglobulinM; MGC9013; sCD40; soluble CD40; T-cell differentiation antigen; Tumor necrosis factor receptor superfamily member 5; tumor necrosis factor receptor superfamily, member 5

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Gene Aliases: AI326936; Bp50; Cd40; GP39; HIGM1; IGM; IMD3; p50; T-BAM; Tnfrsf5; TRAP

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UniProt ID: (Mouse) Q99NE2

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Entrez Gene ID: (Mouse) 21939

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Function(s)

antigen binding protein binding enzyme binding ubiquitin protein ligase binding transmembrane signal receptor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD40 Monoclonal Antibody (1C10), Super Bright™ 436, eBioscience™

CD40 Antibody (62-0401-82) in Flow

Product Details

62-0401-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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