Invitrogen

CD40 Monoclonal Antibody (HM40-3), Functional Grade, eBioscience™

FIGURE: 1 / 15

CD40 Antibody (16-0402-82) in Flow

Staining of BALB/c splenocytes with 0.5 µg of FG Armenian Hamster IgG isotype control (Product # 16-4888-81) (open histogram) or 0.5 µg of Anti-Mouse/Rat CD40 Functional Grade Purified (filled histogram) followed by Anti-Armenian Hamster IgG FITC (Product # 11-4111).Total viable cells were used for analysis.

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Product Details

16-0402-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg/test

View 24 publications 24 publications

Functional Assay (Functional)

Assay-Dependent

View 6 publications 6 publications

Inhibition Assays (IA)

View 1 publication 1 publication

In vitro Assay (IV)

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Armenian hamster / IgM, kappa

Class

Monoclonal

Type

Antibody

Clone

HM40-3

Conjugate

Functional Grade Functional Grade Functional Grade

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468945

Product Specific Information

Description: The HM40-3 monoclonal antibody reacts with mouse and rat CD40, a 45-50 kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by mouse B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development/maturation by inducing Ig isotype switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39), its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation.

Applications Reported: The HM40-3 antibody has been reported for use in flow cytometric analysis. HM40-3 has also been reported in inhibition of binding of soluble CD154 to CD40 and in vitro activation of B cells.

Applications Tested: The HM40-3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

The CD40 antigen is a single chain glycoprotein that is known to be a member of the tumor necrosis factor/nerve growth factor superfamily and shows a significant homology to the Hodgkin's disease associated antigen, CD30. CD40 is present on all B cells except plasma cells and is also found on some epithelial cells, carcinomas and lymphoid dendritic cells. CD40 has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of CD40, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with CD40 and serves as a mediator of the signal transduction. The interaction of CD40 and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Two alternatively spliced transcript variants of CD40 encoding distinct isoforms have been reported. Diseases associated with CD40 dysfunction include Type 3 Hyper-Igm immunodeficiency and CD40 ligand deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Carvacrol protects mice against LPS-induced sepsis and attenuates inflammatory response in macrophages by modulating the ERK1/2 pathway.

Scientific reports

Yan C,Kuang W,Jin L,Wang R,Niu L,Xie C,Ding J,Liao Y,Wang L,Wan H,Ma G

Published figure using CD40 monoclonal antibody (Product # 16-0402-82) in Flow Cytometry

Mon Aug 07 00:00:00 EDT 2023

Curcumin-dependent phenotypic transformation of microglia mediates resistance to pseudorabies-induced encephalitis.

Veterinary research

Feng L,Luo G,Li Y,Zhang C,Liu Y,Liu Y,Chen H,He D,Zhu Y,Gan L

Published figure using CD40 monoclonal antibody (Product # 16-0402-82) in Flow Cytometry

Tue Mar 14 00:00:00 EDT 2023

An Aged/Autoimmune B-cell Program Defines the Early Transformation of Extranodal Lymphomas.

Cancer discovery

Venturutti L,Rivas MA,Pelzer BW,Flümann R,Hansen J,Karagiannidis I,Xia M,McNally DR,Isshiki Y,Lytle A,Teater M,Chin CR,Meydan C,Knittel G,Ricker E,Mason CE,Ye X,Pan-Hammarström Q,Steidl C,Scott DW,Reinhardt HC,Pernis AB,Béguelin W,Melnick AM

16-0402-82 was used in Sample Prep to show that B cells expressing Myd88L252P (MYD88L265P murine equivalent) activate, proliferate, and differentiate with minimal T-cell costimulation.

Mon Jan 09 00:00:00 EST 2023

Balance between immunoregulatory B cells and plasma cells drives pancreatic tumor immunity.

Cell reports. Medicine

Mirlekar B,Wang Y,Li S,Zhou M,Entwistle S,De Buysscher T,Morrison A,Herrera G,Harris C,Vincent BG,Ting JP,Rashid N,Kim WY,Yeh JJ,Pylayeva-Gupta Y

16-0402-82 was used in Flow Cytometry to argue that B cell effector dysfunction in cancer can be due to an active systemic suppression program that can be targeted to synergize with T cell-directed immunotherapy.

Tue Sep 20 00:00:00 EDT 2022

PP2Cδ Controls the Differentiation and Function of Dendritic Cells Through Regulating the NSD2/mTORC2/ACLY Pathway.

Frontiers in immunology

Lv N,Jin S,Liang Z,Wu X,Kang Y,Su L,Dong Y,Wang B,Ma T,Shi L

Published figure using CD40 monoclonal antibody (Product # 16-0402-82) in Flow Cytometry

Mon Feb 28 00:00:00 EST 2022

AMPK-mediated phosphorylation on 53BP1 promotes c-NHEJ.

Cell reports

Jiang Y,Dong Y,Luo Y,Jiang S,Meng FL,Tan M,Li J,Zang Y

16-0402-82 was used in Western Blot to demonstrate that AMPK is a regulator of 53BP1 and controls c-NHEJ choice by phospho-regulation.

Tue Feb 16 00:00:00 EST 2021

ERCC6L2 promotes DNA orientation-specific recombination in mammalian cells.

Cell research

Liu X,Liu T,Shang Y,Dai P,Zhang W,Lee BJ,Huang M,Yang D,Wu Q,Liu LD,Zheng X,Zhou BO,Dong J,Yeap LS,Hu J,Xiao T,Zha S,Casellas R,Liu XS,Meng FL

16-0402-85 was used in CSR assay to identify the Snf2-family helicase-like ERCC6L2 as a factor channel chromatin breaks to deletional CSR in lymphocytes.

Tue Sep 01 00:00:00 EDT 2020

E4BP4-mediated inhibition of T follicular helper cell differentiation is compromised in autoimmune diseases.

The Journal of clinical investigation

Wang Z,Zhao M,Yin J,Liu L,Hu L,Huang Y,Liu A,Ouyang J,Min X,Rao S,Zhou W,Wu H,Yoshimura A,Lu Q

16-0402 was used in Flow cytometry/Cell sorting to study the mediatory role of the adenovirus E4BP4 within Tfh cell differentiation and study the immunological changes seen within patients with systemic lupus erythematosus.

Wed Jul 01 00:00:00 EDT 2020

Conditional antibody expression to avoid central B cell deletion in humanized HIV-1 vaccine mouse models.

Proceedings of the National Academy of Sciences of the United States of America

Tian M,McGovern K,Cheng HL,Waddicor P,Rieble L,Dao M,Chen Y,Kimble MT,Cantor E,Manfredonia N,Judson R,Chapdelaine-Williams A,Cain DW,Haynes BF,Alt FW

16-0402-85 was used in Flow Cytometry to test a vaccine which circumvents B cell developmental blocks.

Tue Apr 07 00:00:00 EDT 2020

REV7 is required for processing AID initiated DNA lesions in activated B cells.

Nature communications

Yang D,Sun Y,Chen J,Zhang Y,Fan S,Huang M,Xie X,Cai Y,Shang Y,Gui T,Sun L,Hu J,Dong J,Yeap LS,Wang X,Xiao W,Meng FL

16-0402-85 was used in In vitro Assay to identify that REV7-depedent TLS across UNG-processed apurinic/apyrimidinic sites is required for cell survival upon AID/APOBEC deamination.

Thu Jun 04 00:00:00 EDT 2020

REV7 is required for processing AID initiated DNA lesions in activated B cells.

Nature communications

Yang D,Sun Y,Chen J,Zhang Y,Fan S,Huang M,Xie X,Cai Y,Shang Y,Gui T,Sun L,Hu J,Dong J,Yeap LS,Wang X,Xiao W,Meng FL

16-0402-85 was used in In vitro Assay to identify that REV7-depedent TLS across UNG-processed apurinic/apyrimidinic sites is required for cell survival upon AID/APOBEC deamination.

Thu Jun 04 00:00:00 EDT 2020

REV7 is required for processing AID initiated DNA lesions in activated B cells.

Nature communications

Yang D,Sun Y,Chen J,Zhang Y,Fan S,Huang M,Xie X,Cai Y,Shang Y,Gui T,Sun L,Hu J,Dong J,Yeap LS,Wang X,Xiao W,Meng FL

16-0402-85 was used in In vitro Assay to identify that REV7-depedent TLS across UNG-processed apurinic/apyrimidinic sites is required for cell survival upon AID/APOBEC deamination.

Thu Jun 04 00:00:00 EDT 2020

Molecular bases for HOIPINs-mediated inhibition of LUBAC and innate immune responses.

Communications biology

Oikawa D,Sato Y,Ohtake F,Komakura K,Hanada K,Sugawara K,Terawaki S,Mizukami Y,Phuong HT,Iio K,Obika S,Fukushi M,Irie T,Tsuruta D,Sakamoto S,Tanaka K,Saeki Y,Fukai S,Tokunaga F

16-0402 was used in Functional assays to show that HOIPINs down-regulate not only the proinflammatory cytokine-induced canonical NF-κB pathway, but also various pathogen-associated molecular pattern-induced antiviral pathways.

Fri Apr 03 00:00:00 EDT 2020

TET enzymes control antibody production and shape the mutational landscape in germinal centre B cells.

The FEBS journal

Schoeler K,Aufschnaiter A,Messner S,Derudder E,Herzog S,Villunger A,Rajewsky K,Labi V

Published figure using CD40 monoclonal antibody (Product # 16-0402-82) in Flow Cytometry

Sun Sep 01 00:00:00 EDT 2019

The B Cell Activation-Induced miR-183 Cluster Plays a Minimal Role in Canonical Primary Humoral Responses.

Journal of immunology (Baltimore, Md. : 1950)

Pucella JN,Cols M,Yen WF,Xu S,Chaudhuri J

16-0402-82 was used in Flow Cytometry to investigate the role of miR-182 in plasmablast differentiation.

Fri Mar 01 00:00:00 EST 2019

cGAS/STING/TBK1/IRF3 Signaling Pathway Activates BMDCs Maturation Following Mycobacterium bovis Infection.

International journal of molecular sciences

Li Q,Liu C,Yue R,El-Ashram S,Wang J,He X,Zhao D,Zhou X,Xu L

Published figure using CD40 monoclonal antibody (Product # 16-0402-82) in Flow Cytometry

Tue Feb 19 00:00:00 EST 2019

Distinct Requirements of CHD4 during B Cell Development and Antibody Response.

Cell reports

Yen WF,Sharma R,Cols M,Lau CM,Chaudhry A,Chowdhury P,Yewdell WT,Vaidyanathan B,Sun A,Coffre M,Pucella JN,Chen CC,Jasin M,Sun JC,Rudensky AY,Koralov SB,Chaudhuri J

16-0402 was used in Flow cytometry/Cell sorting to reveal distinct roles for CHD4 in B cell development and CSR and links the H3K9me3 epigenetic mark with AID recruitment to the Igh locus.

Tue Apr 30 00:00:00 EDT 2019

The RNA-binding protein ROD1/PTBP3 cotranscriptionally defines AID-loading sites to mediate antibody class switch in mammalian genomes.

Cell research

Chen J,Cai Z,Bai M,Yu X,Zhang C,Cao C,Hu X,Wang L,Su R,Wang D,Wang L,Yao Y,Ye R,Hou B,Yu Y,Yu S,Li J,Xue Y

16-0402 was used in Functional assays to identify an RNA-binding protein that is both required and sufficient to define activation-induced cytidine deaminase-binding sites genome-wide in activated B cells.

Mon Oct 01 00:00:00 EDT 2018

MRI Is a DNA Damage Response Adaptor during Classical Non-homologous End Joining.

Molecular cell

Hung PJ,Johnson B,Chen BR,Byrum AK,Bredemeyer AL,Yewdell WT,Johnson TE,Lee BJ,Deivasigamani S,Hindi I,Amatya P,Gross ML,Paull TT,Pisapia DJ,Chaudhuri J,Petrini JJH,Mosammaparast N,Amarasinghe GK,Zha S,Tyler JK,Sleckman BP

16-0402 was used in Flow cytometry/Cell sorting to investigate if in G1-phase XRCC4 and the XRCC4-like factor-deficient lymphocytes, the modulator of retrovirus infection is required for cNHEJ-mediated repair of DNA double-strand breaks generated by RAG and the I-PpoI endonuclease.

Thu Jul 19 00:00:00 EDT 2018

Environmental sensing by mature B cells is controlled by the transcription factors PU.1 and SpiB.

Nature communications

Willis SN,Tellier J,Liao Y,Trezise S,Light A,O'Donnell K,Garrett-Sinha LA,Shi W,Tarlinton DM,Nutt SL

Published figure using CD40 monoclonal antibody (Product # 16-0402-82) in Flow Cytometry

Fri Nov 10 00:00:00 EST 2017

Der f 31, a novel allergen from Dermatophagoides farinae, activates epithelial cells and enhances lung-resident group 2 innate lymphoid cells.

Scientific reports

Wang H,Lin J,Zeng L,Ouyang C,Ran P,Yang P,Liu Z

Published figure using CD40 monoclonal antibody (Product # 16-0402-82) in Flow Cytometry

Thu Aug 17 00:00:00 EDT 2017

Ligase I and ligase III mediate the DNA double-strand break ligation in alternative end-joining.

Proceedings of the National Academy of Sciences of the United States of America

Lu G,Duan J,Shu S,Wang X,Gao L,Guo J,Zhang Y

16-0402 was used in Flow cytometry/Cell sorting to dissect the roles of multiple DNA ligases in mediating the last step of alternative end-joining, and reveal the specificity of different ligase-containing complexes.

Tue Feb 02 00:00:00 EST 2016

Redundant function of DNA ligase 1 and 3 in alternative end-joining during immunoglobulin class switch recombination.

Proceedings of the National Academy of Sciences of the United States of America

Masani S,Han L,Meek K,Yu K

16-0402 was used in Flow cytometry/Cell sorting to demonstrate redundant function of Lig1 and Lig3 in resolving switch region double-strand breaks during class switch recombination.

Tue Feb 02 00:00:00 EST 2016

Semaphorin 4C: A Novel Component of B-Cell Polarization in Th2-Driven Immune Responses.

Frontiers in immunology

Xue D,Desjardins M,Kaufman GN,Béland M,Al-Tamemi S,Ahmed E,Tao S,Friedel RH,Mourad W,Mazer BD

16-0402 was used in Functional assays to report that Semaphorin 4C is inducible in human and murine B-cells and may be important for normal B-cell development.

Wed Nov 20 00:00:00 EST 2019

Thiol peroxiredoxin, a novel allergen from Bombyx mori, modulates functions of macrophages and dendritic cells.

American journal of translational research

Wang H,Hu W,Liang Z,Zeng L,Li J,Yan H,Yang P,Liu Z,Wang L

Published figure using CD40 monoclonal antibody (Product # 16-0402-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

Checkpoint kinase 2 is required for efficient immunoglobulin diversification.

Cell cycle (Georgetown, Tex.)

Davari K,Frankenberger S,Schmidt A,Tomi NS,Jungnickel B

16-0402 was used in Flow cytometry/Cell sorting to investigate the role of checkpoint kinase 2 in immunoglobulin diversification through regulation of Ig hypermutation, class switching and gene conversion.

Thu Sep 10 00:00:00 EDT 2015

p38 MAPK-inhibited dendritic cells induce superior antitumour immune responses and overcome regulatory T-cell-mediated immunosuppression.

Nature communications

Lu Y,Zhang M,Wang S,Hong B,Wang Z,Li H,Zheng Y,Yang J,Davis RE,Qian J,Hou J,Yi Q

16-0402 was used in Inhibition experiments to assess the anti-tumour immune response induced by p38 MAPK-inhibited dendritic cells, and its ability to overcome regulatory T-cell-mediated immunosuppression.

Tue Jun 24 00:00:00 EDT 2014

DNA ligase I is not essential for mammalian cell viability.

Cell reports

Han L,Masani S,Hsieh CL,Yu K

16-0402 was used in Flow cytometry/Cell sorting to demonstrate that DNA ligase I is not absolutely required for cellular DNA replication and repair.

Thu Apr 24 00:00:00 EDT 2014

MicroRNA-17~92 plays a causative role in lymphomagenesis by coordinating multiple oncogenic pathways.

The EMBO journal

Jin HY,Oda H,Lai M,Skalsky RL,Bethel K,Shepherd J,Kang SG,Liu WH,Sabouri-Ghomi M,Cullen BR,Rajewsky K,Xiao C

16-0402 was used in Functional assays to show that elevated miR-17∼92 expression is sufficient to drive lymphomagenesis and, conversely, miR-17∼92 is essential for Myc-mediated lymphoma development.

Wed Aug 28 00:00:00 EDT 2013

A Complex Relationship between TRAF3 and Non-Canonical NF-κB2 Activation in B Lymphocytes.

Frontiers in immunology

Lin WW,Hildebrand JM,Bishop GA

16-0402 was used in Immunoprecipitation to conclude that there is not a simple linear relationship in B lymphocytes between relative levels of cellular TRAF3, induced TRAF3 degradation, NIK activation, and NF-κB2 activation.

Mon Jan 06 00:00:00 EST 2014

Apurinic/apyrimidinic endonuclease 1 is the essential nuclease during immunoglobulin class switch recombination.

Molecular and cellular biology

Masani S,Han L,Yu K

Mon Apr 01 00:00:00 EDT 2013

Apurinic/apyrimidinic endonuclease 1 is the essential nuclease during immunoglobulin class switch recombination.

Molecular and cellular biology

Masani S,Han L,Yu K

Mon Apr 01 00:00:00 EDT 2013

DNA damage activates a complex transcriptional response in murine lymphocytes that includes both physiological and cancer-predisposition programs.

BMC genomics

Innes CL,Hesse JE,Palii SS,Helmink BA,Holub AJ,Sleckman BP,Paules RS

Published figure using CD40 monoclonal antibody (Product # 16-0402-82) in Flow Cytometry

Tue Mar 12 00:00:00 EDT 2013

X-ray repair cross-complementing protein 1 (XRCC1) deficiency enhances class switch recombination and is permissive for alternative end joining.

Proceedings of the National Academy of Sciences of the United States of America

Han L,Mao W,Yu K

16-0402 was used in in vitro experiments to determine the role of x-ray repair cross-complementing protein 1 (XRCC1) in alternative end joining.

Tue Mar 20 00:00:00 EDT 2012

Immunogenicity and protective capacity of a virosomal respiratory syncytial virus vaccine adjuvanted with monophosphoryl lipid A in mice.

PloS one

Kamphuis T,Meijerhof T,Stegmann T,Lederhofer J,Wilschut J,de Haan A

Published figure using CD40 monoclonal antibody (Product # 16-0402-82) in Flow Cytometry

Wed Sep 19 00:00:00 EDT 2012

Overlapping activation-induced cytidine deaminase hotspot motifs in Ig class-switch recombination.

Proceedings of the National Academy of Sciences of the United States of America

Han L,Masani S,Yu K

16-0402 was used in Flow cytometry/Cell sorting to identify that the overlap of activation-induced cytidine deaminase hotspots at WGCW sites on the top and bottom strands is critical for Ig class-switch recombination.

Tue Jul 12 00:00:00 EDT 2011

Alternative end-joining catalyzes robust IgH locus deletions and translocations in the combined absence of ligase 4 and Ku70.

Proceedings of the National Academy of Sciences of the United States of America

Boboila C,Jankovic M,Yan CT,Wang JH,Wesemann DR,Zhang T,Fazeli A,Feldman L,Nussenzweig A,Nussenzweig M,Alt FW

16-0402 was used in Functional assays to study whether internal switch deletions and switch-region translocations occur in the complete absence of a single classical nonhomologous end-joining component.

Tue Feb 16 00:00:00 EST 2010

Antitumoral immune response by recruitment and expansion of dendritic cells in tumors infected with telomerase-dependent oncolytic viruses.

Cancer research

Edukulla R,Woller N,Mundt B,Knocke S,Gürlevik E,Saborowski M,Malek N,Manns MP,Wirth T,Kühnel F,Kubicka S

16-0402 was used in Flow cytometry/Cell sorting to investigate the effect of virotherapy in conjunction with MIP-1alpha and Flt3L in treating primary tumours, showing that triggering dendritic cell and t-cell infiltration can enhance treatment.

Sun Feb 15 00:00:00 EST 2009

Region-specific expression of immunoregulatory proteins on microglia in the healthy CNS.

Glia

de Haas AH,Boddeke HW,Biber K

Published figure using CD40 monoclonal antibody (Product # 16-0402-82) in Flow Cytometry

Sun Jun 01 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: B-cell surface antigen CD40; Bp50; CD antigen CD40; CD40; CD40 antigen, TNF receptor superfamily member 5; CD40L receptor; Immunoglobulin M; ImmunoglobulinM; MGC9013; sCD40; soluble CD40; T-cell differentiation antigen; Tumor necrosis factor receptor superfamily member 5; tumor necrosis factor receptor superfamily, member 5

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Gene Aliases: AI326936; Bp50; Cd40; GP39; HIGM1; IGM; IMD3; p50; T-BAM; Tnfrsf5; TRAP

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UniProt ID: (Mouse) Q99NE2

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Entrez Gene ID: (Rat) 171369, (Mouse) 21939

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Function(s)

antigen binding protein binding enzyme binding ubiquitin protein ligase binding transmembrane signal receptor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD40 Monoclonal Antibody (HM40-3), Functional Grade, eBioscience™

CD40 Antibody (16-0402-82) in Flow

Product Details

16-0402-82

Western Blot (WB)

Flow Cytometry (Flow)

Functional Assay (Functional)

Inhibition Assays (IA)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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