Invitrogen

CD43 Monoclonal Antibody (eBioR2/60), FITC, eBioscience™

FIGURE: 1 / 10

CD43 Antibody (11-0431-82) in Flow

Staining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) APC (Product # 17-0452-82) and 0.06 µg of Rat IgM kappa Isotype Control FITC (Product # 11-4341-82) (left) or 0.06 µg of Anti-Mouse CD43 FITC (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

11-0431-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 21 publications 21 publications

Product Specifications

Species Reactivity

Mouse

Published species

Fish, Mouse

Host/Isotype

Rat / IgM

Recommended Isotype Control

Rat IgM Isotype Control (eBRM), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioR2/60

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465040

Product Specific Information

Description: The eBioR2/60 monoclonal antibody reacts with mouse CD43, also known as leukosialin and sialophorin. CD43 has glycoforms of approximately 115 and 130 kDa, and is expressed by an early B cell subset in BM, thymocytes and mature T cells, NK cells, myeloid lineage cells and platelets. CD43 is reported to bind to CD54 and play a role in adhesion and B cell survival.

While the S7 and 1B11 monoclonal antibody clones detect only certain isoforms of CD43, data suggests that R2/60 detects the expression of both isoforms. It has been demonstrated that R2/60 stains the majority of CD4+ and CD8+ splenocytes, as well as a significant population of CD19+ splenocytes. However, the clones S7 and 1B11 stain only CD4+ and CD8+, or CD19+ splenocytes, respectively. This suggests that S7 and 1B11 recognize distinct epitopes which are differentially expressed by splenic B and T cells, whereas R2/60 recognizes an epitope shared by both isoforms of CD43.

Applications Reported: This eBioR2/60 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioR2/60 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negative charge, expressed on the surface of most hematopoietic cells. CD43 contributes to a repulsive barrier that interferes with cellular adhesion, however, in certain cases also promotes leukocyte aggregation. By interaction with actin-binding proteins ezrin and moesin CD43 plays a regulatory role in remodeling T-cell morphology and regulates cell-cell interactions during lymphocyte traffic. CD43 signaling both enhances LFA-1 adhesiveness and counteracts LFA-1 induction via other receptors. Expression of CD43 causes induction of functionally active tumor suppressor p53 protein, but in case of p53 and ARF deficiency CD43 promotes tumor proliferation and viability. CD43 appears to be an important modulator of leukocyte functions. Diseases associated with CD43 dysfunction include Wiscott-Aldrich Syndrome and Adenoid Basal Cell Carcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

A Hyper-IgM Syndrome Mutation in Activation-Induced Cytidine Deaminase Disrupts G-Quadruplex Binding and Genome-wide Chromatin Localization.

Immunity

Yewdell WT,Kim Y,Chowdhury P,Lau CM,Smolkin RM,Belcheva KT,Fernandez KC,Cols M,Yen WF,Vaidyanathan B,Angeletti D,McDermott AB,Yewdell JW,Sun JC,Chaudhuri J

11-0431-82 was used in Flow cytometry/Cell sorting to indicate a crucial role for G4 binding in AID targeting and suggest that AID activity may extend beyond Ig loci to regulate the expression of genes relevant to the physiology and pathology of activated B cells.

Tue Nov 17 00:00:00 EST 2020

The evolution of zebrafish RAG2 protein is required for adapting to the elevated body temperature of the higher endothermic vertebrates.

Scientific reports

Sun A,Xu K,Liu H,Li H,Shi Y,Zhu X,Liang T,Li X,Cao X,Ji Y,Jiang T,Xu C,Liu X

Published figure using CD43 monoclonal antibody (Product # 11-0431-82) in Flow Cytometry

Thu Mar 05 00:00:00 EST 2020

Modest Declines in Proteome Quality Impair Hematopoietic Stem Cell Self-Renewal.

Cell reports

Hidalgo San Jose L,Sunshine MJ,Dillingham CH,Chua BA,Kruta M,Hong Y,Hatters DM,Signer RAJ

11-0431 was used in Flow cytometry/Cell sorting to show that hematopoietic stem cells (HSCs) are dependent on low protein synthesis to maintain proteostasis, which promotes their self-renewal.

Tue Jan 07 00:00:00 EST 2020

Polyamines Control eIF5A Hypusination, TFEB Translation, and Autophagy to Reverse B Cell Senescence.

Molecular cell

Zhang H,Alsaleh G,Feltham J,Sun Y,Napolitano G,Riffelmacher T,Charles P,Frau L,Hublitz P,Yu Z,Mohammed S,Ballabio A,Balabanov S,Mellor J,Simon AK

11-0431 was used in Flow cytometry/Cell sorting to reveal an unexpected autophagy regulatory mechanism mediated by eIF5A at the translational level, which can be harnessed to reverse immune senescence in humans.

Thu Oct 03 00:00:00 EDT 2019

Mutations in topoisomerase IIβ result in a B cell immunodeficiency.

Nature communications

Broderick L,Yost S,Li D,McGeough MD,Booshehri LM,Guaderrama M,Brydges SD,Kucharova K,Patel NC,Harr M,Hakonarson H,Zackai E,Cowell IG,Austin CA,Hügle B,Gebauer C,Zhang J,Xu X,Wang J,Croker BA,Frazer KA,Putnam CD,Hoffman HM

11-0431-82 was used in Flow Cytometry to identify mutations in type II topoisomerase as a driver of defective proliferation and survival of B2 cells, leading to a B cell immunodeficiency.

Tue Aug 13 00:00:00 EDT 2019

53BP1 cooperation with the REV7-shieldin complex underpins DNA structure-specific NHEJ.

Nature

Ghezraoui H,Oliveira C,Becker JR,Bilham K,Moralli D,Anzilotti C,Fischer R,Deobagkar-Lele M,Sanchiz-Calvo M,Fueyo-Marcos E,Bonham S,Kessler BM,Rottenberg S,Cornall RJ,Green CM,Chapman JR

11-0431 was used in Flow cytometry/Cell sorting to conclude that the 53BP1 pathway therefore comprises distinct double-strand break repair activities within chromatin and single-stranded DNA compartments, which explains both the immunological differences between 53bp1- and Rev7- deficient mice and the context specificity of the pathway.

Wed Aug 01 00:00:00 EDT 2018

53BP1 cooperation with the REV7-shieldin complex underpins DNA structure-specific NHEJ.

Nature

Ghezraoui H,Oliveira C,Becker JR,Bilham K,Moralli D,Anzilotti C,Fischer R,Deobagkar-Lele M,Sanchiz-Calvo M,Fueyo-Marcos E,Bonham S,Kessler BM,Rottenberg S,Cornall RJ,Green CM,Chapman JR

Wed Aug 01 00:00:00 EDT 2018

Inactivation of mTORC1 Signaling in Osterix-Expressing Cells Impairs B-cell Differentiation.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Wang Y,Xiao M,Tao C,Chen J,Wang Z,Yang J,Chen Z,Zou Z,Liu A,Cai D,Jiang Y,Ding C,Li M,Bai X

Published figure using CD43 monoclonal antibody (Product # 11-0431-82) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2018

ORMDL3 Facilitates the Survival of Splenic B Cells via an ATF6α-Endoplasmic Reticulum Stress-Beclin1 Autophagy Regulatory Pathway.

Journal of immunology (Baltimore, Md. : 1950)

Dang J,Bian X,Ma X,Li J,Long F,Shan S,Yuan Q,Xin Q,Li Y,Gao F,Gong Y,Liu Q

11-0431 was used in Flow cytometry/Cell sorting to study the upregulation of orosomucoid-like 3 in both patients with systemic lupus erythematosus and lupus mice compared with controls.

Fri Sep 01 00:00:00 EDT 2017

Loss of IP₃ Receptor-Mediated Ca²⁺ Release in Mouse B Cells Results in Abnormal B Cell Development and Function.

Journal of immunology (Baltimore, Md. : 1950)

Tang H,Wang H,Lin Q,Fan F,Zhang F,Peng X,Fang X,Liu J,Ouyang K

11-0431 was used in Flow cytometry/Cell sorting to determine the function of 1,4,5-trisphosphate receptor-mediated Ca2+ release in B cells.

Sat Jul 15 00:00:00 EDT 2017

lncRNA requirements for mouse acute myeloid leukemia and normal differentiation.

eLife

Delás MJ,Sabin LR,Dolzhenko E,Knott SR,Munera Maravilla E,Jackson BT,Wild SA,Kovacevic T,Stork EM,Zhou M,Erard N,Lee E,Kelley DR,Roth M,Barbosa IA,Zuber J,Rinn JL,Smith AD,Hannon GJ

11-0431 was used in Flow cytometry/Cell sorting to assess lncRNA function via an in vivo RNAi screen in a model of acute myeloid leukemia.

Wed Sep 06 00:00:00 EDT 2017

Myeloid 12/15-LOX regulates B cell numbers and innate immune antibody levels in vivo.

Wellcome open research

Lauder SN,Tyrrell VJ,Allen-Redpath K,Aldrovandi M,Gray D,Collins P,Jones SA,Taylor PR,O'Donnell V

11-0431 was used in Flow cytometry/Cell sorting to discover that 12/15-LOX mediates alterations in B cell numbers and innate immune antibody generation.

Wed Jan 04 00:00:00 EST 2017

Alkylator-Induced and Patient-Derived Xenograft Mouse Models of Therapy-Related Myeloid Neoplasms Model Clinical Disease and Suggest the Presence of Multiple Cell Subpopulations with Leukemia Stem Cell Activity.

PloS one

Jonas BA,Johnson C,Gratzinger D,Majeti R

11-0431 was used in Flow cytometry/Cell sorting to report several new t-AML/MDS mouse models that could potentially be used to further define disease pathogenesis and test novel therapeutics.

Wed Jul 26 00:00:00 EDT 2017

Distinctive Mesenchymal-Parenchymal Cell Pairings Govern B Cell Differentiation in the Bone Marrow.

Stem cell reports

Yu VW,Lymperi S,Oki T,Jones A,Swiatek P,Vasic R,Ferraro F,Scadden DT

11-0431 was used in Flow cytometry/Cell sorting to study the notion that bone marrow is a composite of specialised niches, providing distinctive functional units to regulate hematopoiesis.

Tue Aug 09 00:00:00 EDT 2016

The immunosenescence-related gene Zizimin2 is associated with early bone marrow B cell development and marginal zone B cell formation.

Immunity & ageing : I & A

Matsuda T,Yanase S,Takaoka A,Maruyama M

11-0431 was used in Flow cytometry/Cell sorting to show the immunosenescence-related gene Zizimin2 is associated with early bone marrow B cell development and marginal zone B cell formation.

Mon Mar 02 00:00:00 EST 2015

Rhof promotes murine marginal zone B cell development.

Nagoya journal of medical science

Kishimoto M,Matsuda T,Yanase S,Katsumi A,Suzuki N,Ikejiri M,Takagi A,Ikawa M,Kojima T,Kunishima S,Kiyoi H,Naoe T,Matsushita T,Maruyama M

11-0431 was used in Flow cytometry/Cell sorting to demonstrate that RhoF promotes MZ B cell development in the spleen.

Fri Aug 01 00:00:00 EDT 2014

Rhof promotes murine marginal zone B cell development.

Nagoya journal of medical science

Kishimoto M,Matsuda T,Yanase S,Katsumi A,Suzuki N,Ikejiri M,Takagi A,Ikawa M,Kojima T,Kunishima S,Kiyoi H,Naoe T,Matsushita T,Maruyama M

11-0431 was used in Flow cytometry/Cell sorting to demonstrate that RhoF promotes MZ B cell development in the spleen.

Fri Aug 01 00:00:00 EDT 2014

A mouse model for inducible overexpression of Prdm14 results in rapid-onset and highly penetrant T-cell acute lymphoblastic leukemia (T-ALL).

Disease models & mechanisms

Carofino BL,Ayanga B,Justice MJ

Published figure using CD43 monoclonal antibody (Product # 11-0431-82) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2013

TDP2-dependent non-homologous end-joining protects against topoisomerase II-induced DNA breaks and genome instability in cells and in vivo.

PLoS genetics

Gómez-Herreros F,Romero-Granados R,Zeng Z,Alvarez-Quilón A,Quintero C,Ju L,Umans L,Vermeire L,Huylebroeck D,Caldecott KW,Cortés-Ledesma F

11-0431 was used in Flow cytometry/Cell sorting to reveal TDP2-mediated error-free NHEJ as an efficient and accurate mechanism to repair TOP2-induced DSBs.

Thu Jun 13 00:00:00 EDT 2013

Prolactin levels correlate with abnormal B cell maturation in MRL and MRL/lpr mouse models of systemic lupus erythematosus-like disease.

Clinical & developmental immunology

Legorreta-Haquet MV,Flores-Fernández R,Blanco-Favela F,Fuentes-Pananá EM,Chávez-Sánchez L,Hernández-González R,Tesoro-Cruz E,Arriaga-Pizano L,Chávez-Rueda AK

11-0431 was used in Flow cytometry/Cell sorting to determine the level of PRL receptor expression during bone-marrow B-cell development and assess relevance in disease outcomes in lupus-prone murine models.

Mon Aug 04 00:00:00 EDT 2014

IKKα-mediated signaling circuitry regulates early B lymphopoiesis during hematopoiesis.

Blood

Balkhi MY,Willette-Brown J,Zhu F,Chen Z,Liu S,Guttridge DC,Karin M,Hu Y

11-0431-82 was used in Flow Cytometry to demonstrate that IKKα regulates B-lineage commitment via combined canonical and noncanonical NF-κB transcriptional activities to target Pax5 expression during hematopoiesis.

Thu Jun 07 00:00:00 EDT 2012

Conditional TPM3-ALK and NPM-ALK transgenic mice develop reversible ALK-positive early B-cell lymphoma/leukemia.

Blood

Giuriato S,Foisseau M,Dejean E,Felsher DW,Al Saati T,Demur C,Ragab A,Kruczynski A,Schiff C,Delsol G,Meggetto F

11-0431 was used in Flow cytometry/Cell sorting to develop conditional mouse models for anaplastic lymphoma kinase-induced lymphomagenesis.

Thu May 20 00:00:00 EDT 2010

Homologous recombination is necessary for normal lymphocyte development.

Molecular and cellular biology

Caddle LB,Hasham MG,Schott WH,Shirley BJ,Mills KD

11-0431 was used in Flow cytometry/Cell sorting to demonstrate a key role for HR in lymphoid development and suggest that Xrcc2 defects could underlie some human primary immunodeficiencies.

Tue Apr 01 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: 3E8 antigen; B-cell differentiation antigen LP-3; CD43; Leukocyte sialoglycoprotein; Leukosialin; Ly-48; Lymphocyte antigen 48; Sialophorin; sialophorin gpL115

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Gene Aliases: A630014B01Rik; Cd43; Galgp; Ly-48; Ly48; Spn

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UniProt ID: (Mouse) P15702

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Entrez Gene ID: (Mouse) 20737

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Function(s)

protein binding protein domain specific binding

Process(es)

response to protozoan negative regulation of type IV hypersensitivity positive regulation of protein phosphorylation negative regulation of cell adhesion cell surface receptor signaling pathway T cell costimulation positive regulation of T cell proliferation negative regulation of T cell proliferation positive regulation of tumor necrosis factor biosynthetic process defense response to bacterium negative thymic T cell selection regulation of defense response to virus regulation of immune response negative regulation of T cell activation thymocyte aggregation apoptotic signaling pathway

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD43 Monoclonal Antibody (eBioR2/60), FITC, eBioscience™

CD43 Antibody (11-0431-82) in Flow

Product Details

11-0431-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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